Internal quality assurance of HIL indices on Roche Cobas c702
Automatic assessment of hemoglobin (H), lipaemia (L) and icterus (I) in serum or plasma (HIL indices) is the mainstay for evaluating sample quality. We planned this study to verify whether in-house prepared internal quality control (IQC) materials may be suitable for quality assurance of HIL indices...
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| creator | Lippi, Giuseppe Cadamuro, Janne Danese, Elisa Gelati, Matteo Montagnana, Martina von Meyer, Alexander Salvagno, Gian Luca Simundic, Ana-Maria |
| description | Automatic assessment of hemoglobin (H), lipaemia (L) and icterus (I) in serum or plasma (HIL indices) is the mainstay for evaluating sample quality. We planned this study to verify whether in-house prepared internal quality control (IQC) materials may be suitable for quality assurance of HIL indices. Pools containing different values of each of the three HIL indices were prepared from routine plasma samples, divided in aliquots and frozen at -20°C. Stability of frozen materials was assessed by thawing one aliquot of each pool after different days of freezing (1, 4, 8, 15, 22 and 29), and by measuring HIL indices on baseline fresh samples and frozen-thawed aliquots with Roche Cobas c702. Five fresh liquid IQCs materials were also measured at the same time points. Intra-assay and inter-assay imprecision of HIL indices calculated with commercial IQC materials ranged between 1.1-2.0% and 1.6-3.3%, respectively. When target values of HIL indices were calculated using frozen-thawed aliquots, the inter-assay imprecision of in-house prepared materials was optimal, even better than that of commercial liquid IQCs (H-index, 0.8% versus 1.6%; L-index, 2.2% versus 2.5%; I-index, 0.8% versus 3.3%). In conclusion, in-house prepared IQC materials are cost-effective alternatives to commercial liquid IQCs for HIL quality assurance. |
| doi_str_mv | 10.1371/journal.pone.0200088 |
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We planned this study to verify whether in-house prepared internal quality control (IQC) materials may be suitable for quality assurance of HIL indices. Pools containing different values of each of the three HIL indices were prepared from routine plasma samples, divided in aliquots and frozen at -20°C. Stability of frozen materials was assessed by thawing one aliquot of each pool after different days of freezing (1, 4, 8, 15, 22 and 29), and by measuring HIL indices on baseline fresh samples and frozen-thawed aliquots with Roche Cobas c702. Five fresh liquid IQCs materials were also measured at the same time points. Intra-assay and inter-assay imprecision of HIL indices calculated with commercial IQC materials ranged between 1.1-2.0% and 1.6-3.3%, respectively. When target values of HIL indices were calculated using frozen-thawed aliquots, the inter-assay imprecision of in-house prepared materials was optimal, even better than that of commercial liquid IQCs (H-index, 0.8% versus 1.6%; L-index, 2.2% versus 2.5%; I-index, 0.8% versus 3.3%). In conclusion, in-house prepared IQC materials are cost-effective alternatives to commercial liquid IQCs for HIL quality assurance.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0200088</identifier><identifier>PMID: 29979722</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Assaying ; Automation ; Baltimore oriole ; Bilirubin - blood ; Bilirubin - standards ; Biochemistry ; Biology and Life Sciences ; Blood Chemical Analysis - instrumentation ; Blood Chemical Analysis - standards ; Blood Chemical Analysis - statistics & numerical data ; Blood serum ; Engineering and Technology ; Freezing ; Hemoglobin ; Hemoglobins ; Hemoglobins - analysis ; Hemoglobins - standards ; Hemolysis ; Humans ; Hyperlipidemias - blood ; Hyperlipidemias - diagnosis ; Jaundice - blood ; Jaundice - diagnosis ; Laboratories ; Lipids - blood ; Lipids - standards ; Mathematical analysis ; Medical tests ; Medicine ; Medicine and Health Sciences ; People and places ; Quality assurance ; Quality Control ; Reference Standards ; Research and Analysis Methods ; Stability analysis ; Thawing ; Working groups</subject><ispartof>PloS one, 2018-07, Vol.13 (7), p.e0200088-e0200088</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Lippi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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We planned this study to verify whether in-house prepared internal quality control (IQC) materials may be suitable for quality assurance of HIL indices. Pools containing different values of each of the three HIL indices were prepared from routine plasma samples, divided in aliquots and frozen at -20°C. Stability of frozen materials was assessed by thawing one aliquot of each pool after different days of freezing (1, 4, 8, 15, 22 and 29), and by measuring HIL indices on baseline fresh samples and frozen-thawed aliquots with Roche Cobas c702. Five fresh liquid IQCs materials were also measured at the same time points. Intra-assay and inter-assay imprecision of HIL indices calculated with commercial IQC materials ranged between 1.1-2.0% and 1.6-3.3%, respectively. When target values of HIL indices were calculated using frozen-thawed aliquots, the inter-assay imprecision of in-house prepared materials was optimal, even better than that of commercial liquid IQCs (H-index, 0.8% versus 1.6%; L-index, 2.2% versus 2.5%; I-index, 0.8% versus 3.3%). 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lippi, Giuseppe</au><au>Cadamuro, Janne</au><au>Danese, Elisa</au><au>Gelati, Matteo</au><au>Montagnana, Martina</au><au>von Meyer, Alexander</au><au>Salvagno, Gian Luca</au><au>Simundic, Ana-Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Internal quality assurance of HIL indices on Roche Cobas c702</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-07-06</date><risdate>2018</risdate><volume>13</volume><issue>7</issue><spage>e0200088</spage><epage>e0200088</epage><pages>e0200088-e0200088</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Automatic assessment of hemoglobin (H), lipaemia (L) and icterus (I) in serum or plasma (HIL indices) is the mainstay for evaluating sample quality. We planned this study to verify whether in-house prepared internal quality control (IQC) materials may be suitable for quality assurance of HIL indices. Pools containing different values of each of the three HIL indices were prepared from routine plasma samples, divided in aliquots and frozen at -20°C. Stability of frozen materials was assessed by thawing one aliquot of each pool after different days of freezing (1, 4, 8, 15, 22 and 29), and by measuring HIL indices on baseline fresh samples and frozen-thawed aliquots with Roche Cobas c702. Five fresh liquid IQCs materials were also measured at the same time points. Intra-assay and inter-assay imprecision of HIL indices calculated with commercial IQC materials ranged between 1.1-2.0% and 1.6-3.3%, respectively. When target values of HIL indices were calculated using frozen-thawed aliquots, the inter-assay imprecision of in-house prepared materials was optimal, even better than that of commercial liquid IQCs (H-index, 0.8% versus 1.6%; L-index, 2.2% versus 2.5%; I-index, 0.8% versus 3.3%). In conclusion, in-house prepared IQC materials are cost-effective alternatives to commercial liquid IQCs for HIL quality assurance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29979722</pmid><doi>10.1371/journal.pone.0200088</doi><orcidid>https://orcid.org/0000-0002-2454-0410</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Assaying Automation Baltimore oriole Bilirubin - blood Bilirubin - standards Biochemistry Biology and Life Sciences Blood Chemical Analysis - instrumentation Blood Chemical Analysis - standards Blood Chemical Analysis - statistics & numerical data Blood serum Engineering and Technology Freezing Hemoglobin Hemoglobins Hemoglobins - analysis Hemoglobins - standards Hemolysis Humans Hyperlipidemias - blood Hyperlipidemias - diagnosis Jaundice - blood Jaundice - diagnosis Laboratories Lipids - blood Lipids - standards Mathematical analysis Medical tests Medicine Medicine and Health Sciences People and places Quality assurance Quality Control Reference Standards Research and Analysis Methods Stability analysis Thawing Working groups |
| title | Internal quality assurance of HIL indices on Roche Cobas c702 |
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