Development of a novel real-time polymerase chain reaction assay for the quantitative detection of Nipah virus replicative viral RNA

Nipah virus (NiV) is a highly pathogenic zoonotic paramyxovirus that can result in severe pulmonary disease and fatal encephalitis in humans and is responsible for outbreaks in Bangladesh, Malaysia, Singapore, India and possibly the Philippines. NiV has a negative-sense RNA genome that contains six...

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Veröffentlicht in:	PloS one 2018-06, Vol.13 (6), p.e0199534
Hauptverfasser:	Jensen, Kenneth S, Adams, Ricky, Bennett, Richard S, Bernbaum, John, Jahrling, Peter B, Holbrook, Michael R
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Animals Antibiotics Assaying Biology and Life Sciences Chiroptera Chlorocebus aethiops Diagnosis Encephalitis Fatalities Genes Genetic aspects Genome, Viral Genomes Genomics Henipavirus Infections Lung diseases Microscopy, Electron Nipah virus Nipah Virus - genetics Nucleocapsids Outbreaks Polymerase chain reaction Proteins Real time Real-Time Polymerase Chain Reaction - methods Research and analysis methods Ribonucleic acid RNA RNA viruses RNA, Messenger - genetics RNA, Viral - analysis RNA-directed DNA polymerase Vero Cells Viral Proteins - genetics Viruses Zoonoses
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