Selection and evaluation of reference genes for expression analysis using quantitative real-time PCR in the Asian Ladybird Harmonia axyridis (Coleoptera: Coccinellidae)

Harmonia axyridis (Coleoptera: Coccinellidae) is a polyphagous insect that is an important biological agent used to control agricultural and forestry pests. The role of functional genes in H. axyridis based on quantitative real-time PCR (qRT-PCR) is increasingly well understood to investigate biolog...

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Veröffentlicht in:	PloS one 2018-06, Vol.13 (6), p.e0192521-e0192521
Hauptverfasser:	Qu, Cheng, Wang, Ran, Che, Wunan, Zhu, Xun, Li, Fengqi, Luo, Chen
Format:	Artikel
Sprache:	eng
Schlagworte:	Abiotic factors Actin Agriculture Analytical methods Animals Aphidoidea Biochemistry Biology and Life Sciences Biotic factors Coccinellidae Coleoptera Coleoptera - genetics Coleoptera - metabolism Developmental stages Elongation Evaluation Feeding behavior Forestry Gene expression Gene Expression Regulation Genes Genetic aspects Genomics Glyceraldehyde-3-phosphate dehydrogenase Harmonia axyridis Heat Heat shock proteins Hemiptera High temperature Hsp90 protein Insect Proteins - biosynthesis Insect Proteins - genetics Insects Ladybugs Lepeophtheirus salmonis Lepidoptera Metabolism Molecular biology Noctuidae Pests Photoperiods Physical Sciences Physiological aspects Physiology Polymerase chain reaction Proteins Real time Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reference Standards Research and Analysis Methods Ribosomal proteins Stability analysis Superoxide dismutase
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