Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide
Trichinella spiralis mainly dwells in the muscle tissue of its host and is the main causative agent of trichinellosis in humans. Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, incl...
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| Veröffentlicht in: | PloS one 2018-06, Vol.13 (6), p.e0198205-e0198205 |
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| creator | Wang, Xiaoli Li, Liang Wei, Xing Wang, Yuanyuan Zhang, Hui Shi, Ao Liu, Tao Yang, Xiaodi Fang, Qiang |
| description | Trichinella spiralis mainly dwells in the muscle tissue of its host and is the main causative agent of trichinellosis in humans. Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, including resistance to T. spiralis. Herein, we applied label-free analysis to investigate the effect of sodium nitroprusside (SNP, a NO donor compound) on the proteome of T. spiralis muscle larvae (ML), followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analyses. Of the 1,476 proteins detected in the ML, 121 proteins showed differential expression, including 50 significantly upregulated and 71 downregulated proteins. The functions of the 108 annotated proteins were primarily related to signal transduction, transcription/translation, material metabolism, protein synthesis/assembly/degradation, and stress/defense/antioxidation. Quantitative real-time polymerase chain reaction (qRT-PCR) assay verified that FRMD5 and CUT-1 gene expression levels were significantly increased, while COX2 gene expression level was significantly decreased. GO annotation and KEGG pathway analyses showed that the majority of differentially expressed proteins were mainly involved in the molecular function of the catalytic activity, biological process of the immune system process, metabolic process, cellular component organization, biological adhesion, and cellular component of the macromolecular complex. Our results demonstrate the first comprehensive protein expression profile of the ML in response to NO stress and provide novel references for understanding the potential mechanism underlying the effects of NO on trichinellosis. |
| doi_str_mv | 10.1371/journal.pone.0198205 |
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Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, including resistance to T. spiralis. Herein, we applied label-free analysis to investigate the effect of sodium nitroprusside (SNP, a NO donor compound) on the proteome of T. spiralis muscle larvae (ML), followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analyses. Of the 1,476 proteins detected in the ML, 121 proteins showed differential expression, including 50 significantly upregulated and 71 downregulated proteins. The functions of the 108 annotated proteins were primarily related to signal transduction, transcription/translation, material metabolism, protein synthesis/assembly/degradation, and stress/defense/antioxidation. Quantitative real-time polymerase chain reaction (qRT-PCR) assay verified that FRMD5 and CUT-1 gene expression levels were significantly increased, while COX2 gene expression level was significantly decreased. GO annotation and KEGG pathway analyses showed that the majority of differentially expressed proteins were mainly involved in the molecular function of the catalytic activity, biological process of the immune system process, metabolic process, cellular component organization, biological adhesion, and cellular component of the macromolecular complex. Our results demonstrate the first comprehensive protein expression profile of the ML in response to NO stress and provide novel references for understanding the potential mechanism underlying the effects of NO on trichinellosis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0198205</identifier><identifier>PMID: 29870543</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Annotations ; Biological activity ; Biology ; Biology and Life Sciences ; Biosynthesis ; Caenorhabditis elegans ; Catalysis ; Catalytic activity ; Chemical synthesis ; Cyclooxygenase-2 ; Cytochrome ; Encyclopedias ; Gene expression ; Genomes ; Health aspects ; Helminth Proteins - biosynthesis ; Immune system ; Infections ; Intracellular signalling ; Laboratory animals ; Larva - metabolism ; Larvae ; Macromolecules ; Mass spectrometry ; Medicine and Health Sciences ; Metabolism ; Molecular chains ; Muscle Proteins - biosynthesis ; Muscles ; Muscles - metabolism ; Nitric oxide ; Nitric Oxide - pharmacology ; Parasites ; Parasitology ; Polymerase chain reaction ; Protein biosynthesis ; Protein synthesis ; Protein turnover ; Proteins ; Proteomes ; Proteomics ; Research and Analysis Methods ; Scientific imaging ; Signal transduction ; Single-nucleotide polymorphism ; Sodium ; Sodium nitroprusside ; Transcription ; Trichina worm ; Trichinella ; Trichinella spiralis ; Trichinella spiralis - metabolism ; Trichinellosis ; Trichinosis ; Wang, Liang</subject><ispartof>PloS one, 2018-06, Vol.13 (6), p.e0198205-e0198205</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Wang et al 2018 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bf5b3afa184978f90f68783d0d8cc79c6a9afbd906de4c6236612680139708773</citedby><cites>FETCH-LOGICAL-c692t-bf5b3afa184978f90f68783d0d8cc79c6a9afbd906de4c6236612680139708773</cites><orcidid>0000-0002-6321-6891</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988324/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988324/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29870543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Song, Linsheng</contributor><creatorcontrib>Wang, Xiaoli</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Wei, Xing</creatorcontrib><creatorcontrib>Wang, Yuanyuan</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Shi, Ao</creatorcontrib><creatorcontrib>Liu, Tao</creatorcontrib><creatorcontrib>Yang, Xiaodi</creatorcontrib><creatorcontrib>Fang, Qiang</creatorcontrib><title>Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Trichinella spiralis mainly dwells in the muscle tissue of its host and is the main causative agent of trichinellosis in humans. Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, including resistance to T. spiralis. Herein, we applied label-free analysis to investigate the effect of sodium nitroprusside (SNP, a NO donor compound) on the proteome of T. spiralis muscle larvae (ML), followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analyses. Of the 1,476 proteins detected in the ML, 121 proteins showed differential expression, including 50 significantly upregulated and 71 downregulated proteins. The functions of the 108 annotated proteins were primarily related to signal transduction, transcription/translation, material metabolism, protein synthesis/assembly/degradation, and stress/defense/antioxidation. Quantitative real-time polymerase chain reaction (qRT-PCR) assay verified that FRMD5 and CUT-1 gene expression levels were significantly increased, while COX2 gene expression level was significantly decreased. GO annotation and KEGG pathway analyses showed that the majority of differentially expressed proteins were mainly involved in the molecular function of the catalytic activity, biological process of the immune system process, metabolic process, cellular component organization, biological adhesion, and cellular component of the macromolecular complex. Our results demonstrate the first comprehensive protein expression profile of the ML in response to NO stress and provide novel references for understanding the potential mechanism underlying the effects of NO on trichinellosis.</description><subject>Analysis</subject><subject>Animals</subject><subject>Annotations</subject><subject>Biological activity</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Caenorhabditis elegans</subject><subject>Catalysis</subject><subject>Catalytic activity</subject><subject>Chemical synthesis</subject><subject>Cyclooxygenase-2</subject><subject>Cytochrome</subject><subject>Encyclopedias</subject><subject>Gene expression</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Helminth Proteins - biosynthesis</subject><subject>Immune system</subject><subject>Infections</subject><subject>Intracellular signalling</subject><subject>Laboratory animals</subject><subject>Larva - 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metabolism</subject><subject>Trichinellosis</subject><subject>Trichinosis</subject><subject>Wang, Liang</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjEnTfN0Iy-LHwMKKrt6GNE07GTrNmKTL7L_31OkuU9kL6UWb9Hnf9Lw9J8teYrTEhOMPGz-EXnfLne_tEmEpCkQfZadYkmLBCkQeHz2fZM9i3CBEiWDsaXZSSMERLclpVn0LPlm_dSbX4HYbXcx9k6e1zYON4B3tuL4Ozqxdb7tO53Hngu6A2w7RdDbvdLjRNk8-t3vf2t4PMe9dAkXu9662z7Mnje6ifTHdz7Kfnz9dX3xdXF59WV2cXy4Mk0VaVA2tiG40FqXkopGoYYILUqNaGMOlYVrqpqolYrUtDSsIY7hgAmEiORKck7Ps9cF31_mopniiglhQSTHDAojVgai93qhdcFsdbpXXTv3d8KFVOiQHRSlccloZXVDKZGl0LQiVUoiClNwwXFPw-jidNlRbWxvbJ0hlZjp_07u1av2NomBDihIM3k0Gwf8ebExq66IZE-4tRDh-N0ZMYowAffMP-nB1E9VqKMD1jYdzzWiqzmkJBTHKR2r5AAVXbaEJoJcaB_szwfuZAJhk96nVQ4xq9eP7_7NXv-bs2yN2bXWX1tF3Q3LQdHOwPIAm-BiDbe5DxkiNo3CXhhpHQU2jALJXxz_oXnTX--QPF0kDWA</recordid><startdate>20180605</startdate><enddate>20180605</enddate><creator>Wang, Xiaoli</creator><creator>Li, Liang</creator><creator>Wei, Xing</creator><creator>Wang, Yuanyuan</creator><creator>Zhang, Hui</creator><creator>Shi, Ao</creator><creator>Liu, Tao</creator><creator>Yang, Xiaodi</creator><creator>Fang, Qiang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6321-6891</orcidid></search><sort><creationdate>20180605</creationdate><title>Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide</title><author>Wang, Xiaoli ; Li, Liang ; Wei, Xing ; Wang, Yuanyuan ; Zhang, Hui ; Shi, Ao ; Liu, Tao ; Yang, Xiaodi ; Fang, Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bf5b3afa184978f90f68783d0d8cc79c6a9afbd906de4c6236612680139708773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Annotations</topic><topic>Biological activity</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Caenorhabditis elegans</topic><topic>Catalysis</topic><topic>Catalytic activity</topic><topic>Chemical synthesis</topic><topic>Cyclooxygenase-2</topic><topic>Cytochrome</topic><topic>Encyclopedias</topic><topic>Gene expression</topic><topic>Genomes</topic><topic>Health aspects</topic><topic>Helminth Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiaoli</au><au>Li, Liang</au><au>Wei, Xing</au><au>Wang, Yuanyuan</au><au>Zhang, Hui</au><au>Shi, Ao</au><au>Liu, Tao</au><au>Yang, Xiaodi</au><au>Fang, Qiang</au><au>Song, Linsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-06-05</date><risdate>2018</risdate><volume>13</volume><issue>6</issue><spage>e0198205</spage><epage>e0198205</epage><pages>e0198205-e0198205</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Trichinella spiralis mainly dwells in the muscle tissue of its host and is the main causative agent of trichinellosis in humans. Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, including resistance to T. spiralis. Herein, we applied label-free analysis to investigate the effect of sodium nitroprusside (SNP, a NO donor compound) on the proteome of T. spiralis muscle larvae (ML), followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analyses. Of the 1,476 proteins detected in the ML, 121 proteins showed differential expression, including 50 significantly upregulated and 71 downregulated proteins. The functions of the 108 annotated proteins were primarily related to signal transduction, transcription/translation, material metabolism, protein synthesis/assembly/degradation, and stress/defense/antioxidation. Quantitative real-time polymerase chain reaction (qRT-PCR) assay verified that FRMD5 and CUT-1 gene expression levels were significantly increased, while COX2 gene expression level was significantly decreased. GO annotation and KEGG pathway analyses showed that the majority of differentially expressed proteins were mainly involved in the molecular function of the catalytic activity, biological process of the immune system process, metabolic process, cellular component organization, biological adhesion, and cellular component of the macromolecular complex. Our results demonstrate the first comprehensive protein expression profile of the ML in response to NO stress and provide novel references for understanding the potential mechanism underlying the effects of NO on trichinellosis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29870543</pmid><doi>10.1371/journal.pone.0198205</doi><tpages>e0198205</tpages><orcidid>https://orcid.org/0000-0002-6321-6891</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-06, Vol.13 (6), p.e0198205-e0198205 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2050451618 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Annotations Biological activity Biology Biology and Life Sciences Biosynthesis Caenorhabditis elegans Catalysis Catalytic activity Chemical synthesis Cyclooxygenase-2 Cytochrome Encyclopedias Gene expression Genomes Health aspects Helminth Proteins - biosynthesis Immune system Infections Intracellular signalling Laboratory animals Larva - metabolism Larvae Macromolecules Mass spectrometry Medicine and Health Sciences Metabolism Molecular chains Muscle Proteins - biosynthesis Muscles Muscles - metabolism Nitric oxide Nitric Oxide - pharmacology Parasites Parasitology Polymerase chain reaction Protein biosynthesis Protein synthesis Protein turnover Proteins Proteomes Proteomics Research and Analysis Methods Scientific imaging Signal transduction Single-nucleotide polymorphism Sodium Sodium nitroprusside Transcription Trichina worm Trichinella Trichinella spiralis Trichinella spiralis - metabolism Trichinellosis Trichinosis Wang, Liang |
| title | Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T05%3A39%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Proteomic%20analysis%20of%20the%20response%20of%20Trichinella%20spiralis%20muscle%20larvae%20to%20exogenous%20nitric%20oxide&rft.jtitle=PloS%20one&rft.au=Wang,%20Xiaoli&rft.date=2018-06-05&rft.volume=13&rft.issue=6&rft.spage=e0198205&rft.epage=e0198205&rft.pages=e0198205-e0198205&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0198205&rft_dat=%3Cgale_plos_%3EA541476578%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2050451618&rft_id=info:pmid/29870543&rft_galeid=A541476578&rft_doaj_id=oai_doaj_org_article_1475bca255694cad83599882347c61d5&rfr_iscdi=true |