Distinct domains of Escherichia coli IgaA connect envelope stress sensing and down-regulation of the Rcs phosphorelay across subcellular compartments
In enterobacteria, the Rcs system (Regulator of capsule synthesis) monitors envelope integrity and induces a stress response when damages occur in the outer membrane or in the peptidoglycan layer. Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer...
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description | In enterobacteria, the Rcs system (Regulator of capsule synthesis) monitors envelope integrity and induces a stress response when damages occur in the outer membrane or in the peptidoglycan layer. Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer reactions. Being particularly complex, Rcs also involves the outer membrane lipoprotein RcsF and the inner membrane essential protein IgaA (Intracellular growth attenuator). RcsF and IgaA, which are located upstream of the phosphorelay, are required for normal Rcs functioning. Here, we establish the stress-dependent formation of a complex between RcsF and the periplasmic domain of IgaA as the molecular signal triggering Rcs. Moreover, molecular dissection of IgaA reveals that its negative regulatory role on Rcs is mostly carried by its first N-terminal cytoplasmic domain. Altogether, our results support a model in which IgaA regulates Rcs activation by playing a direct role in the transfer of signals from the cell envelope to the cytoplasm. This remarkable feature further distinguishes Rcs from other envelope stress response systems. |
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Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer reactions. Being particularly complex, Rcs also involves the outer membrane lipoprotein RcsF and the inner membrane essential protein IgaA (Intracellular growth attenuator). RcsF and IgaA, which are located upstream of the phosphorelay, are required for normal Rcs functioning. Here, we establish the stress-dependent formation of a complex between RcsF and the periplasmic domain of IgaA as the molecular signal triggering Rcs. Moreover, molecular dissection of IgaA reveals that its negative regulatory role on Rcs is mostly carried by its first N-terminal cytoplasmic domain. Altogether, our results support a model in which IgaA regulates Rcs activation by playing a direct role in the transfer of signals from the cell envelope to the cytoplasm. This remarkable feature further distinguishes Rcs from other envelope stress response systems.</description><identifier>ISSN: 1553-7404</identifier><identifier>ISSN: 1553-7390</identifier><identifier>EISSN: 1553-7404</identifier><identifier>DOI: 10.1371/journal.pgen.1007398</identifier><identifier>PMID: 29852010</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibiotics ; Antimicrobial agents ; Bacteria ; Biology and Life Sciences ; Cellular stress response ; Cytoplasm ; E coli ; Escherichia coli ; Gene expression ; Genes ; Genetic aspects ; Immunoglobulins ; Kinases ; Lipoproteins ; Medicine and Health Sciences ; Membrane proteins ; Observations ; Peptidoglycans ; Phosphorylation ; Physiological aspects ; Proteins ; Research and Analysis Methods ; Salmonella ; Sensors ; Signal transduction ; Supervision</subject><ispartof>PLoS genetics, 2018-05, Vol.14 (5), p.e1007398-e1007398</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Hussein et al. 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This remarkable feature further distinguishes Rcs from other envelope stress response systems.</description><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Cellular stress response</subject><subject>Cytoplasm</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Immunoglobulins</subject><subject>Kinases</subject><subject>Lipoproteins</subject><subject>Medicine and Health Sciences</subject><subject>Membrane proteins</subject><subject>Observations</subject><subject>Peptidoglycans</subject><subject>Phosphorylation</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Salmonella</subject><subject>Sensors</subject><subject>Signal 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domains of Escherichia coli IgaA connect envelope stress sensing and down-regulation of the Rcs phosphorelay across subcellular compartments</title><author>Hussein, Nahla A ; Cho, Seung-Hyun ; Laloux, Géraldine ; Siam, Rania ; Collet, Jean-François</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c726t-3955fe596b3b9afe57fbcb7a634d0103a9a2ae9d0faa7dcea9a09645b753cc7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Biology and Life Sciences</topic><topic>Cellular stress response</topic><topic>Cytoplasm</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Immunoglobulins</topic><topic>Kinases</topic><topic>Lipoproteins</topic><topic>Medicine and Health Sciences</topic><topic>Membrane proteins</topic><topic>Observations</topic><topic>Peptidoglycans</topic><topic>Phosphorylation</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Salmonella</topic><topic>Sensors</topic><topic>Signal transduction</topic><topic>Supervision</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hussein, Nahla A</creatorcontrib><creatorcontrib>Cho, Seung-Hyun</creatorcontrib><creatorcontrib>Laloux, Géraldine</creatorcontrib><creatorcontrib>Siam, Rania</creatorcontrib><creatorcontrib>Collet, Jean-François</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Canada</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception 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Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer reactions. Being particularly complex, Rcs also involves the outer membrane lipoprotein RcsF and the inner membrane essential protein IgaA (Intracellular growth attenuator). RcsF and IgaA, which are located upstream of the phosphorelay, are required for normal Rcs functioning. Here, we establish the stress-dependent formation of a complex between RcsF and the periplasmic domain of IgaA as the molecular signal triggering Rcs. Moreover, molecular dissection of IgaA reveals that its negative regulatory role on Rcs is mostly carried by its first N-terminal cytoplasmic domain. Altogether, our results support a model in which IgaA regulates Rcs activation by playing a direct role in the transfer of signals from the cell envelope to the cytoplasm. 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subjects | Antibiotics Antimicrobial agents Bacteria Biology and Life Sciences Cellular stress response Cytoplasm E coli Escherichia coli Gene expression Genes Genetic aspects Immunoglobulins Kinases Lipoproteins Medicine and Health Sciences Membrane proteins Observations Peptidoglycans Phosphorylation Physiological aspects Proteins Research and Analysis Methods Salmonella Sensors Signal transduction Supervision |
title | Distinct domains of Escherichia coli IgaA connect envelope stress sensing and down-regulation of the Rcs phosphorelay across subcellular compartments |
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