Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis
Bronchiectasis (BE) in children is common in some communities including Indigenous children in Australia. Relatively little is known about the nature of systemic inflammation in these children, especially the contribution of specific pro-inflammatory and cytotoxic lymphocyte subsets: T-cells, natura...
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| creator | Hodge, G Upham, J W Chang, A B Baines, K J Yerkovich, S T Pizzutto, S J Hodge, S |
| description | Bronchiectasis (BE) in children is common in some communities including Indigenous children in Australia. Relatively little is known about the nature of systemic inflammation in these children, especially the contribution of specific pro-inflammatory and cytotoxic lymphocyte subsets: T-cells, natural killer (NK) cells and NKT-like cells. We have shown that these cells produce increased cytotoxic (granzyme b and perforin) and inflammatory (IFNγ and TNFα) mediators in several adult chronic lung diseases and hypothesised that similar changes would be evident in children with BE.
Intracellular cytotoxic mediators perforin and granzyme b and pro-inflammatory cytokines were measured in T cell subsets, NKT-like and NK cells from blood and bronchoalveolar samples from 12 children with BE and 10 aged-matched control children using flow cytometry.
There was a significant increase in the percentage of CD8+ T cells and T and NKT-like subsets expressing perforin/granzyme and IFNγ and TNFα in blood in BE compared with controls. There was a further increase in the percentage of pro-inflammatory cytotoxic T cells in Indigenous compared with non-Indigenous children. There was no change in any of these mediators in BAL.
Childhood bronchiectasis is associated with increased systemic pro-inflammatory/cytotoxic lymphocytes in the peripheral blood. Future studies need to examine the extent to which elevated levels of pro-inflammatory cytotoxic cells predict future co-morbidities. |
| doi_str_mv | 10.1371/journal.pone.0133695 |
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Intracellular cytotoxic mediators perforin and granzyme b and pro-inflammatory cytokines were measured in T cell subsets, NKT-like and NK cells from blood and bronchoalveolar samples from 12 children with BE and 10 aged-matched control children using flow cytometry.
There was a significant increase in the percentage of CD8+ T cells and T and NKT-like subsets expressing perforin/granzyme and IFNγ and TNFα in blood in BE compared with controls. There was a further increase in the percentage of pro-inflammatory cytotoxic T cells in Indigenous compared with non-Indigenous children. There was no change in any of these mediators in BAL.
Childhood bronchiectasis is associated with increased systemic pro-inflammatory/cytotoxic lymphocytes in the peripheral blood. Future studies need to examine the extent to which elevated levels of pro-inflammatory cytotoxic cells predict future co-morbidities.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0133695</identifier><identifier>PMID: 26258716</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aboriginal Australians ; Alveoli ; Asthma ; Atherosclerosis ; Australia ; Blood ; Bronchiectasis ; Bronchiectasis - blood ; Bronchoalveolar Lavage Fluid ; Bronchus ; Cardiovascular disease ; Case-Control Studies ; CD8 antigen ; Child ; Child, Preschool ; Children ; Childrens health ; Chronic obstructive pulmonary disease ; Cytokines ; Cytotoxicity ; Family medical history ; Female ; Flow Cytometry ; Granzyme B ; Granzymes - blood ; Haemophilus influenzae ; Hospitals ; Humans ; Infant ; Inflammation ; Inflammation - blood ; Interferon ; Interferon-gamma - blood ; Interferon-gamma - metabolism ; Killer Cells, Natural - cytology ; Lung diseases ; Lymphocytes ; Lymphocytes T ; Male ; Medical research ; Medicine ; Natural killer cells ; Osteoporosis ; Perforin ; Perforin - blood ; Peripheral blood ; Population Groups ; Sociodemographics ; Streptococcus infections ; T cells ; T-Lymphocytes - cytology ; T-Lymphocytes, Cytotoxic - cytology ; Transplants & implants ; Tumor necrosis factor ; Tumor Necrosis Factor-alpha - blood ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; γ-Interferon</subject><ispartof>PloS one, 2015-08, Vol.10 (8), p.e0133695-e0133695</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Hodge et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Hodge et al 2015 Hodge et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-7ab489525a8fb300a31b9c38650ca368f6c3336b36296f484683586e6f69a1553</citedby><cites>FETCH-LOGICAL-c692t-7ab489525a8fb300a31b9c38650ca368f6c3336b36296f484683586e6f69a1553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4530946/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4530946/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26258716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Fabbri, Leonardo M.</contributor><creatorcontrib>Hodge, G</creatorcontrib><creatorcontrib>Upham, J W</creatorcontrib><creatorcontrib>Chang, A B</creatorcontrib><creatorcontrib>Baines, K J</creatorcontrib><creatorcontrib>Yerkovich, S T</creatorcontrib><creatorcontrib>Pizzutto, S J</creatorcontrib><creatorcontrib>Hodge, S</creatorcontrib><title>Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bronchiectasis (BE) in children is common in some communities including Indigenous children in Australia. Relatively little is known about the nature of systemic inflammation in these children, especially the contribution of specific pro-inflammatory and cytotoxic lymphocyte subsets: T-cells, natural killer (NK) cells and NKT-like cells. We have shown that these cells produce increased cytotoxic (granzyme b and perforin) and inflammatory (IFNγ and TNFα) mediators in several adult chronic lung diseases and hypothesised that similar changes would be evident in children with BE.
Intracellular cytotoxic mediators perforin and granzyme b and pro-inflammatory cytokines were measured in T cell subsets, NKT-like and NK cells from blood and bronchoalveolar samples from 12 children with BE and 10 aged-matched control children using flow cytometry.
There was a significant increase in the percentage of CD8+ T cells and T and NKT-like subsets expressing perforin/granzyme and IFNγ and TNFα in blood in BE compared with controls. There was a further increase in the percentage of pro-inflammatory cytotoxic T cells in Indigenous compared with non-Indigenous children. There was no change in any of these mediators in BAL.
Childhood bronchiectasis is associated with increased systemic pro-inflammatory/cytotoxic lymphocytes in the peripheral blood. Future studies need to examine the extent to which elevated levels of pro-inflammatory cytotoxic cells predict future co-morbidities.</description><subject>Aboriginal Australians</subject><subject>Alveoli</subject><subject>Asthma</subject><subject>Atherosclerosis</subject><subject>Australia</subject><subject>Blood</subject><subject>Bronchiectasis</subject><subject>Bronchiectasis - blood</subject><subject>Bronchoalveolar Lavage Fluid</subject><subject>Bronchus</subject><subject>Cardiovascular disease</subject><subject>Case-Control Studies</subject><subject>CD8 antigen</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Childrens health</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Family medical history</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Granzyme B</subject><subject>Granzymes - blood</subject><subject>Haemophilus influenzae</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infant</subject><subject>Inflammation</subject><subject>Inflammation - blood</subject><subject>Interferon</subject><subject>Interferon-gamma - blood</subject><subject>Interferon-gamma - metabolism</subject><subject>Killer Cells, Natural - cytology</subject><subject>Lung diseases</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Natural killer cells</subject><subject>Osteoporosis</subject><subject>Perforin</subject><subject>Perforin - blood</subject><subject>Peripheral blood</subject><subject>Population Groups</subject><subject>Sociodemographics</subject><subject>Streptococcus infections</subject><subject>T cells</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes, Cytotoxic - cytology</subject><subject>Transplants & implants</subject><subject>Tumor necrosis factor</subject><subject>Tumor Necrosis Factor-alpha - blood</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>γ-Interferon</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uP1CAUxxujcdfVb2C0iYnRh5mlUCi8mOxOvEyyyRpvr4RSOmVCYQSqO99e6nQ3U7MPhgfI4Xf-5wIny54XYFmgqjjfusFbYZY7Z9USFAgRhh9kpwVDcEEgQA-PzifZkxC2AGBECXmcnUACMa0KcpqJtZVeiaCa_LPyetcpL0x-aZxLBu8Wa9sa0fciOr8_X-2ji-5Gy_xq3-86J_dRhVzbfNVp03hl8986dvmld1Z2Wskogg5Ps0etMEE9m_az7PuH999WnxZX1x_Xq4urhSQMxkUl6pIyDLGgbY0AEKiomUz5YiAFIrQlEqUaa0QgI21JS0IRpkSRljBRYIzOspcH3Z1xgU_dCRyCErGSMEYTsT4QjRNbvvO6F37PndD8r8H5DRc-amkUhzUFNajqMZOSkZKhBmLUCEFFBUs1ar2bog11rxqpbEyNm4nOb6zu-Mb94iVGIOWTBN5MAt79HFSIvNdBKmOEVW4IvKhAeuWKYpjQV_-g91c3URuRCtC2dSmuHEX5RQkxKChCo9byHiqtRvVapq_U6mSfObydOSQmqpu4EUMIfP31y_-z1z_m7OsjtlPCxC44M0TtbJiD5QGU3oXgVXvX5ALwcRJuu8HHSeDTJCS3F8cPdOd0-_XRH0jDAjU</recordid><startdate>20150810</startdate><enddate>20150810</enddate><creator>Hodge, G</creator><creator>Upham, J W</creator><creator>Chang, A B</creator><creator>Baines, K J</creator><creator>Yerkovich, S T</creator><creator>Pizzutto, S J</creator><creator>Hodge, S</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150810</creationdate><title>Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis</title><author>Hodge, G ; Upham, J W ; Chang, A B ; Baines, K J ; Yerkovich, S T ; Pizzutto, S J ; Hodge, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-7ab489525a8fb300a31b9c38650ca368f6c3336b36296f484683586e6f69a1553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aboriginal Australians</topic><topic>Alveoli</topic><topic>Asthma</topic><topic>Atherosclerosis</topic><topic>Australia</topic><topic>Blood</topic><topic>Bronchiectasis</topic><topic>Bronchiectasis - blood</topic><topic>Bronchoalveolar Lavage Fluid</topic><topic>Bronchus</topic><topic>Cardiovascular disease</topic><topic>Case-Control Studies</topic><topic>CD8 antigen</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Childrens health</topic><topic>Chronic obstructive pulmonary disease</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Family medical history</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Granzyme B</topic><topic>Granzymes - blood</topic><topic>Haemophilus influenzae</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infant</topic><topic>Inflammation</topic><topic>Inflammation - blood</topic><topic>Interferon</topic><topic>Interferon-gamma - blood</topic><topic>Interferon-gamma - metabolism</topic><topic>Killer Cells, Natural - cytology</topic><topic>Lung diseases</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Natural killer cells</topic><topic>Osteoporosis</topic><topic>Perforin</topic><topic>Perforin - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hodge, G</au><au>Upham, J W</au><au>Chang, A B</au><au>Baines, K J</au><au>Yerkovich, S T</au><au>Pizzutto, S J</au><au>Hodge, S</au><au>Fabbri, Leonardo M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-08-10</date><risdate>2015</risdate><volume>10</volume><issue>8</issue><spage>e0133695</spage><epage>e0133695</epage><pages>e0133695-e0133695</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bronchiectasis (BE) in children is common in some communities including Indigenous children in Australia. Relatively little is known about the nature of systemic inflammation in these children, especially the contribution of specific pro-inflammatory and cytotoxic lymphocyte subsets: T-cells, natural killer (NK) cells and NKT-like cells. We have shown that these cells produce increased cytotoxic (granzyme b and perforin) and inflammatory (IFNγ and TNFα) mediators in several adult chronic lung diseases and hypothesised that similar changes would be evident in children with BE.
Intracellular cytotoxic mediators perforin and granzyme b and pro-inflammatory cytokines were measured in T cell subsets, NKT-like and NK cells from blood and bronchoalveolar samples from 12 children with BE and 10 aged-matched control children using flow cytometry.
There was a significant increase in the percentage of CD8+ T cells and T and NKT-like subsets expressing perforin/granzyme and IFNγ and TNFα in blood in BE compared with controls. There was a further increase in the percentage of pro-inflammatory cytotoxic T cells in Indigenous compared with non-Indigenous children. There was no change in any of these mediators in BAL.
Childhood bronchiectasis is associated with increased systemic pro-inflammatory/cytotoxic lymphocytes in the peripheral blood. Future studies need to examine the extent to which elevated levels of pro-inflammatory cytotoxic cells predict future co-morbidities.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26258716</pmid><doi>10.1371/journal.pone.0133695</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-08, Vol.10 (8), p.e0133695-e0133695 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2043946998 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aboriginal Australians Alveoli Asthma Atherosclerosis Australia Blood Bronchiectasis Bronchiectasis - blood Bronchoalveolar Lavage Fluid Bronchus Cardiovascular disease Case-Control Studies CD8 antigen Child Child, Preschool Children Childrens health Chronic obstructive pulmonary disease Cytokines Cytotoxicity Family medical history Female Flow Cytometry Granzyme B Granzymes - blood Haemophilus influenzae Hospitals Humans Infant Inflammation Inflammation - blood Interferon Interferon-gamma - blood Interferon-gamma - metabolism Killer Cells, Natural - cytology Lung diseases Lymphocytes Lymphocytes T Male Medical research Medicine Natural killer cells Osteoporosis Perforin Perforin - blood Peripheral blood Population Groups Sociodemographics Streptococcus infections T cells T-Lymphocytes - cytology T-Lymphocytes, Cytotoxic - cytology Transplants & implants Tumor necrosis factor Tumor Necrosis Factor-alpha - blood Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-TNF Tumor necrosis factor-α γ-Interferon |
| title | Increased Peripheral Blood Pro-Inflammatory/Cytotoxic Lymphocytes in Children with Bronchiectasis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-29T13%3A32%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20Peripheral%20Blood%20Pro-Inflammatory/Cytotoxic%20Lymphocytes%20in%20Children%20with%20Bronchiectasis&rft.jtitle=PloS%20one&rft.au=Hodge,%20G&rft.date=2015-08-10&rft.volume=10&rft.issue=8&rft.spage=e0133695&rft.epage=e0133695&rft.pages=e0133695-e0133695&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0133695&rft_dat=%3Cgale_plos_%3EA425018332%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2043946998&rft_id=info:pmid/26258716&rft_galeid=A425018332&rft_doaj_id=oai_doaj_org_article_2b80b07bb300496493d253daa8a724e8&rfr_iscdi=true |