Distribution, Numbers, and Diversity of ESBL-Producing E. coli in the Poultry Farm Environment
This study aimed to discern the contribution of poultry farms to the contamination of the environment with ESBL-producing Escherichia coli and therewith, potentially to the spread of these bacteria to humans and other animals. ESBL-producing E. coli were detected at all investigated laying hen farms...
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| Veröffentlicht in: | PloS one 2015-08, Vol.10 (8), p.e0135402-e0135402 |
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| creator | Blaak, Hetty van Hoek, Angela H A M Hamidjaja, Raditijo A van der Plaats, Rozemarijn Q J Kerkhof-de Heer, Lianne de Roda Husman, Ana Maria Schets, Franciska M |
| description | This study aimed to discern the contribution of poultry farms to the contamination of the environment with ESBL-producing Escherichia coli and therewith, potentially to the spread of these bacteria to humans and other animals. ESBL-producing E. coli were detected at all investigated laying hen farms (n = 5) and broiler farms (n = 3) in 65% (46/71) and 81% (57/70) of poultry faeces samples, respectively. They were detected in rinse water and run-off water (21/26; 81%), other farm animals (11/14; 79%), dust (21/35; 60%), surface water adjacent to farms (20/35; 57%), soil (48/87; 55%), on flies (11/73; 15%), and in barn air (2/33; 6%). The highest prevalence and concentrations in the outdoor environment were observed in soil of free-range areas at laying hen farms (100% of samples positive, geometric mean concentration 2.4×10(4) cfu/kg), and surface waters adjacent to broiler farms during, or shortly after, cleaning between production rounds (91% of samples positive, geometric mean concentration 1.9×10(2) cfu/l). The diversity of ESBL-producing E. coli variants with respect to sequence type, phylogenetic group, ESBL-genotype and antibiotic resistance profile was high, especially on broiler farms where on average 16 different variants were detected, and the average Simpson's Indices of diversity (SID; 1-D) were 0.93 and 0.94 among flock and environmental isolates respectively. At laying hen farms on average nine variants were detected, with SIDs of 0.63 (flock isolates) and 0.77 (environmental isolates). Sixty percent of environmental isolates were identical to flock isolates at the same farm. The highest proportions of 'flock variants' were observed in dust (94%), run-off gullies (82%), and barn air (67%), followed by surface water (57%), soil (56%), flies (50%) and other farm animals (35%).The introduction of ESBL-producing E. coli from poultry farms to the environment may pose a health risk if these bacteria reach places where people may become exposed. |
| doi_str_mv | 10.1371/journal.pone.0135402 |
| format | Article |
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ESBL-producing E. coli were detected at all investigated laying hen farms (n = 5) and broiler farms (n = 3) in 65% (46/71) and 81% (57/70) of poultry faeces samples, respectively. They were detected in rinse water and run-off water (21/26; 81%), other farm animals (11/14; 79%), dust (21/35; 60%), surface water adjacent to farms (20/35; 57%), soil (48/87; 55%), on flies (11/73; 15%), and in barn air (2/33; 6%). The highest prevalence and concentrations in the outdoor environment were observed in soil of free-range areas at laying hen farms (100% of samples positive, geometric mean concentration 2.4×10(4) cfu/kg), and surface waters adjacent to broiler farms during, or shortly after, cleaning between production rounds (91% of samples positive, geometric mean concentration 1.9×10(2) cfu/l). The diversity of ESBL-producing E. coli variants with respect to sequence type, phylogenetic group, ESBL-genotype and antibiotic resistance profile was high, especially on broiler farms where on average 16 different variants were detected, and the average Simpson's Indices of diversity (SID; 1-D) were 0.93 and 0.94 among flock and environmental isolates respectively. At laying hen farms on average nine variants were detected, with SIDs of 0.63 (flock isolates) and 0.77 (environmental isolates). Sixty percent of environmental isolates were identical to flock isolates at the same farm. The highest proportions of 'flock variants' were observed in dust (94%), run-off gullies (82%), and barn air (67%), followed by surface water (57%), soil (56%), flies (50%) and other farm animals (35%).The introduction of ESBL-producing E. coli from poultry farms to the environment may pose a health risk if these bacteria reach places where people may become exposed.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0135402</identifier><identifier>PMID: 26270644</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agricultural pollution ; Agriculture ; Animals ; Antibiotic resistance ; Antibiotics ; Bacteria ; Beta lactamases ; beta-Lactam Resistance - genetics ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; Cephalosporins ; Chickens ; Chickens - microbiology ; Contamination ; Drug resistance ; Dust ; E coli ; Environments ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; Farm buildings ; Farms ; Food contamination & poisoning ; Genotypes ; Gullies ; Health care ; Health risks ; Livestock ; Livestock farms ; Muscidae ; Phylogeny ; Plasmids ; Poultry ; Poultry - microbiology ; Poultry farming ; Poultry industry ; Public health ; Runoff ; Soil contamination ; Soil water ; Soils ; Statistics ; Surface water ; Trends ; Urinary tract diseases ; Urinary tract infections ; Urogenital system ; Wash water ; Water resources ; Zoonoses</subject><ispartof>PloS one, 2015-08, Vol.10 (8), p.e0135402-e0135402</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Blaak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Blaak et al 2015 Blaak et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c809t-36fe8f8c768ba79bc613b4cbb6a39246a0b23ca367e15f427f12c6915ecd33093</citedby><cites>FETCH-LOGICAL-c809t-36fe8f8c768ba79bc613b4cbb6a39246a0b23ca367e15f427f12c6915ecd33093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536194/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536194/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26270644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blaak, Hetty</creatorcontrib><creatorcontrib>van Hoek, Angela H A M</creatorcontrib><creatorcontrib>Hamidjaja, Raditijo A</creatorcontrib><creatorcontrib>van der Plaats, Rozemarijn Q J</creatorcontrib><creatorcontrib>Kerkhof-de Heer, Lianne</creatorcontrib><creatorcontrib>de Roda Husman, Ana Maria</creatorcontrib><creatorcontrib>Schets, Franciska M</creatorcontrib><title>Distribution, Numbers, and Diversity of ESBL-Producing E. coli in the Poultry Farm Environment</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study aimed to discern the contribution of poultry farms to the contamination of the environment with ESBL-producing Escherichia coli and therewith, potentially to the spread of these bacteria to humans and other animals. ESBL-producing E. coli were detected at all investigated laying hen farms (n = 5) and broiler farms (n = 3) in 65% (46/71) and 81% (57/70) of poultry faeces samples, respectively. They were detected in rinse water and run-off water (21/26; 81%), other farm animals (11/14; 79%), dust (21/35; 60%), surface water adjacent to farms (20/35; 57%), soil (48/87; 55%), on flies (11/73; 15%), and in barn air (2/33; 6%). The highest prevalence and concentrations in the outdoor environment were observed in soil of free-range areas at laying hen farms (100% of samples positive, geometric mean concentration 2.4×10(4) cfu/kg), and surface waters adjacent to broiler farms during, or shortly after, cleaning between production rounds (91% of samples positive, geometric mean concentration 1.9×10(2) cfu/l). 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blaak, Hetty</au><au>van Hoek, Angela H A M</au><au>Hamidjaja, Raditijo A</au><au>van der Plaats, Rozemarijn Q J</au><au>Kerkhof-de Heer, Lianne</au><au>de Roda Husman, Ana Maria</au><au>Schets, Franciska M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution, Numbers, and Diversity of ESBL-Producing E. coli in the Poultry Farm Environment</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-08-13</date><risdate>2015</risdate><volume>10</volume><issue>8</issue><spage>e0135402</spage><epage>e0135402</epage><pages>e0135402-e0135402</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study aimed to discern the contribution of poultry farms to the contamination of the environment with ESBL-producing Escherichia coli and therewith, potentially to the spread of these bacteria to humans and other animals. ESBL-producing E. coli were detected at all investigated laying hen farms (n = 5) and broiler farms (n = 3) in 65% (46/71) and 81% (57/70) of poultry faeces samples, respectively. They were detected in rinse water and run-off water (21/26; 81%), other farm animals (11/14; 79%), dust (21/35; 60%), surface water adjacent to farms (20/35; 57%), soil (48/87; 55%), on flies (11/73; 15%), and in barn air (2/33; 6%). The highest prevalence and concentrations in the outdoor environment were observed in soil of free-range areas at laying hen farms (100% of samples positive, geometric mean concentration 2.4×10(4) cfu/kg), and surface waters adjacent to broiler farms during, or shortly after, cleaning between production rounds (91% of samples positive, geometric mean concentration 1.9×10(2) cfu/l). The diversity of ESBL-producing E. coli variants with respect to sequence type, phylogenetic group, ESBL-genotype and antibiotic resistance profile was high, especially on broiler farms where on average 16 different variants were detected, and the average Simpson's Indices of diversity (SID; 1-D) were 0.93 and 0.94 among flock and environmental isolates respectively. At laying hen farms on average nine variants were detected, with SIDs of 0.63 (flock isolates) and 0.77 (environmental isolates). Sixty percent of environmental isolates were identical to flock isolates at the same farm. The highest proportions of 'flock variants' were observed in dust (94%), run-off gullies (82%), and barn air (67%), followed by surface water (57%), soil (56%), flies (50%) and other farm animals (35%).The introduction of ESBL-producing E. coli from poultry farms to the environment may pose a health risk if these bacteria reach places where people may become exposed.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26270644</pmid><doi>10.1371/journal.pone.0135402</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-08, Vol.10 (8), p.e0135402-e0135402 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2043769764 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Agricultural pollution Agriculture Animals Antibiotic resistance Antibiotics Bacteria Beta lactamases beta-Lactam Resistance - genetics beta-Lactamases - genetics beta-Lactamases - metabolism Cephalosporins Chickens Chickens - microbiology Contamination Drug resistance Dust E coli Environments Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Farm buildings Farms Food contamination & poisoning Genotypes Gullies Health care Health risks Livestock Livestock farms Muscidae Phylogeny Plasmids Poultry Poultry - microbiology Poultry farming Poultry industry Public health Runoff Soil contamination Soil water Soils Statistics Surface water Trends Urinary tract diseases Urinary tract infections Urogenital system Wash water Water resources Zoonoses |
| title | Distribution, Numbers, and Diversity of ESBL-Producing E. coli in the Poultry Farm Environment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T14%3A49%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distribution,%20Numbers,%20and%20Diversity%20of%20ESBL-Producing%20E.%20coli%20in%20the%20Poultry%20Farm%20Environment&rft.jtitle=PloS%20one&rft.au=Blaak,%20Hetty&rft.date=2015-08-13&rft.volume=10&rft.issue=8&rft.spage=e0135402&rft.epage=e0135402&rft.pages=e0135402-e0135402&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0135402&rft_dat=%3Cgale_plos_%3EA425311286%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2043769764&rft_id=info:pmid/26270644&rft_galeid=A425311286&rft_doaj_id=oai_doaj_org_article_c1555940a31e4cac8f5859ab9e27d6ce&rfr_iscdi=true |