Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing
Adipose-derived stem cells (ADSCs) transplant has been reported to be a potential treatment for burn wounds. However, the effects of autogenicity and allogenicity of ADSCs on burn wound healing have not been investigated and the method for using ADSCs still needs to be established. This study compar...
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| creator | Chang, Yu-Wei Wu, Yi-Chia Huang, Shu-Hung Wang, Hui-Min David Kuo, Yur-Ren Lee, Su-Shin |
| description | Adipose-derived stem cells (ADSCs) transplant has been reported to be a potential treatment for burn wounds. However, the effects of autogenicity and allogenicity of ADSCs on burn wound healing have not been investigated and the method for using ADSCs still needs to be established. This study compared the healing effects of autologous and allogenic ADSCs and determined an optimal method of using ADSCs to treat acute burn wounds. Experiments were performed in 20 male Wistar rats (weight, 176-250 g; age, 6-7 weeks). Two identical full-thickness burn wounds (radius, 4 mm) were created in each rat. ADSCs harvested from inguinal area and characterized by their high multipotency were injected into burn wounds in the original donor rats (autologous ADSCs group) or in other rats (allogenic ADSCs group). The injection site was either the wound center or the four corners 0.5 cm from the wound edge. The reduction of burn surface areas in the two experimental groups and in control group were evaluated with Image J software for 15 days post-wounding to determine the wound healing rates. Wound healing was significantly faster in the autologous ADSCs group compared to both the allogenic ADSCs group (p |
| doi_str_mv | 10.1371/journal.pone.0197744 |
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However, the effects of autogenicity and allogenicity of ADSCs on burn wound healing have not been investigated and the method for using ADSCs still needs to be established. This study compared the healing effects of autologous and allogenic ADSCs and determined an optimal method of using ADSCs to treat acute burn wounds. Experiments were performed in 20 male Wistar rats (weight, 176-250 g; age, 6-7 weeks). Two identical full-thickness burn wounds (radius, 4 mm) were created in each rat. ADSCs harvested from inguinal area and characterized by their high multipotency were injected into burn wounds in the original donor rats (autologous ADSCs group) or in other rats (allogenic ADSCs group). The injection site was either the wound center or the four corners 0.5 cm from the wound edge. The reduction of burn surface areas in the two experimental groups and in control group were evaluated with Image J software for 15 days post-wounding to determine the wound healing rates. Wound healing was significantly faster in the autologous ADSCs group compared to both the allogenic ADSCs group (p<0.05) and control group (p<0.05). Wound healing in the allogenic ADSC group did not significantly differ from that in control group. Notably, ADSC injections 0.5cm from the wound edge showed significantly improved healing compared to ADSCs injections in the wound center (p<0.05). This study demonstrated the therapeutic efficacy of ADSCs in treating acute burn wounds in rats. However, only autologous ADSCs improved healing in acute burn wounds; allogenic ADSCs did not. This study further determined a superior location of using ADSCs injections to treat burn wounds including the injection site. Future studies will replicate the experiment in a larger and long-term scale burn wounds in higher mammalian models to facilitate ADSCs therapy in burn wound clinical practice.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0197744</identifier><identifier>PMID: 29787581</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipocytes - cytology ; Animal models ; Animals ; Autografts ; Biology and Life Sciences ; Bone marrow ; Burns ; Burns - therapy ; Cancer therapies ; Care and treatment ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Diabetes ; Hospitals ; Injection ; Male ; Medical prognosis ; Medical research ; Medicine ; Medicine and Health Sciences ; Methods ; Plastic surgery ; Rats ; Rats, Wistar ; Skin ; Software ; Stem cell transplantation ; Stem Cell Transplantation - methods ; Stem cells ; Stem Cells - cytology ; Therapeutic applications ; Transplantation, Autologous ; Transplantation, Homologous ; Transplants & implants ; Wound Healing</subject><ispartof>PloS one, 2018-05, Vol.13 (5), p.e0197744-e0197744</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Chang et al 2018 Chang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-312550b4f0edd588168588cbd585f5a29aadb7159c8a31a64e44245f3214ec7b3</citedby><cites>FETCH-LOGICAL-c692t-312550b4f0edd588168588cbd585f5a29aadb7159c8a31a64e44245f3214ec7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963767/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963767/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29787581$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hamblin, Michael R</contributor><creatorcontrib>Chang, Yu-Wei</creatorcontrib><creatorcontrib>Wu, Yi-Chia</creatorcontrib><creatorcontrib>Huang, Shu-Hung</creatorcontrib><creatorcontrib>Wang, Hui-Min David</creatorcontrib><creatorcontrib>Kuo, Yur-Ren</creatorcontrib><creatorcontrib>Lee, Su-Shin</creatorcontrib><title>Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Adipose-derived stem cells (ADSCs) transplant has been reported to be a potential treatment for burn wounds. However, the effects of autogenicity and allogenicity of ADSCs on burn wound healing have not been investigated and the method for using ADSCs still needs to be established. This study compared the healing effects of autologous and allogenic ADSCs and determined an optimal method of using ADSCs to treat acute burn wounds. Experiments were performed in 20 male Wistar rats (weight, 176-250 g; age, 6-7 weeks). Two identical full-thickness burn wounds (radius, 4 mm) were created in each rat. ADSCs harvested from inguinal area and characterized by their high multipotency were injected into burn wounds in the original donor rats (autologous ADSCs group) or in other rats (allogenic ADSCs group). The injection site was either the wound center or the four corners 0.5 cm from the wound edge. The reduction of burn surface areas in the two experimental groups and in control group were evaluated with Image J software for 15 days post-wounding to determine the wound healing rates. Wound healing was significantly faster in the autologous ADSCs group compared to both the allogenic ADSCs group (p<0.05) and control group (p<0.05). Wound healing in the allogenic ADSC group did not significantly differ from that in control group. Notably, ADSC injections 0.5cm from the wound edge showed significantly improved healing compared to ADSCs injections in the wound center (p<0.05). This study demonstrated the therapeutic efficacy of ADSCs in treating acute burn wounds in rats. However, only autologous ADSCs improved healing in acute burn wounds; allogenic ADSCs did not. This study further determined a superior location of using ADSCs injections to treat burn wounds including the injection site. Future studies will replicate the experiment in a larger and long-term scale burn wounds in higher mammalian models to facilitate ADSCs therapy in burn wound clinical practice.</description><subject>Adipocytes - cytology</subject><subject>Animal models</subject><subject>Animals</subject><subject>Autografts</subject><subject>Biology and Life Sciences</subject><subject>Bone marrow</subject><subject>Burns</subject><subject>Burns - therapy</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Diabetes</subject><subject>Hospitals</subject><subject>Injection</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Plastic surgery</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Skin</subject><subject>Software</subject><subject>Stem cell transplantation</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Therapeutic applications</subject><subject>Transplantation, Autologous</subject><subject>Transplantation, Homologous</subject><subject>Transplants & implants</subject><subject>Wound Healing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1DAUx4so7jr6DUQLgujDjM09fRGGxcvAwoI38Cmk6WknQ9uMTTrufntTp7tMZR8kkIST3_knOZckeY6yFSICvdu5oe90s9q7DlYZyoWg9EFyjnKClxxn5OHJ_ix54v0uyxiRnD9OznAupGASnSc_10Nwjavd4FPdlWnnQqqbaIAOrEl1affOw7KE3h6gTH2ANjXQND617b53B0i1GQKkRXxM-tsNUWILurFd_TR5VOnGw7NpXSTfP374dvF5eXn1aXOxvlwanuOwJAgzlhW0yqAsmZSIyzibIu5ZxTTOtS4LgVhupCZIcwqUYsoqghEFIwqySF4edfeN82qKilc4o1hgKUUWic2RKJ3eqX1vW93fKKet-mtwfa10H6xpQImCaakRlJwYShDXJkcAUafisioqiFrvp9uGooXSQBd63cxE5yed3araHRTLORFcRIE3k0Dvfg3gg2qtHyOqO4hJGN9NkCQ45mqRvPoHvf93E1Xr-AHbVS7ea0ZRtWYUEY4Jl5Fa3UPFUUJrTSyhykb7zOHtzCEyAa5DrQfv1ebrl_9nr37M2dcn7FgrYetdMwTrOj8H6RE0vfO-h-ouyChTYwfcRkONHaCmDohuL04TdOd0W_LkD15_ARs</recordid><startdate>20180522</startdate><enddate>20180522</enddate><creator>Chang, Yu-Wei</creator><creator>Wu, Yi-Chia</creator><creator>Huang, Shu-Hung</creator><creator>Wang, Hui-Min David</creator><creator>Kuo, Yur-Ren</creator><creator>Lee, Su-Shin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20180522</creationdate><title>Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing</title><author>Chang, Yu-Wei ; Wu, Yi-Chia ; Huang, Shu-Hung ; Wang, Hui-Min David ; Kuo, Yur-Ren ; Lee, Su-Shin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-312550b4f0edd588168588cbd585f5a29aadb7159c8a31a64e44245f3214ec7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adipocytes - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Yu-Wei</au><au>Wu, Yi-Chia</au><au>Huang, Shu-Hung</au><au>Wang, Hui-Min David</au><au>Kuo, Yur-Ren</au><au>Lee, Su-Shin</au><au>Hamblin, Michael R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-05-22</date><risdate>2018</risdate><volume>13</volume><issue>5</issue><spage>e0197744</spage><epage>e0197744</epage><pages>e0197744-e0197744</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Adipose-derived stem cells (ADSCs) transplant has been reported to be a potential treatment for burn wounds. However, the effects of autogenicity and allogenicity of ADSCs on burn wound healing have not been investigated and the method for using ADSCs still needs to be established. This study compared the healing effects of autologous and allogenic ADSCs and determined an optimal method of using ADSCs to treat acute burn wounds. Experiments were performed in 20 male Wistar rats (weight, 176-250 g; age, 6-7 weeks). Two identical full-thickness burn wounds (radius, 4 mm) were created in each rat. ADSCs harvested from inguinal area and characterized by their high multipotency were injected into burn wounds in the original donor rats (autologous ADSCs group) or in other rats (allogenic ADSCs group). The injection site was either the wound center or the four corners 0.5 cm from the wound edge. The reduction of burn surface areas in the two experimental groups and in control group were evaluated with Image J software for 15 days post-wounding to determine the wound healing rates. Wound healing was significantly faster in the autologous ADSCs group compared to both the allogenic ADSCs group (p<0.05) and control group (p<0.05). Wound healing in the allogenic ADSC group did not significantly differ from that in control group. Notably, ADSC injections 0.5cm from the wound edge showed significantly improved healing compared to ADSCs injections in the wound center (p<0.05). This study demonstrated the therapeutic efficacy of ADSCs in treating acute burn wounds in rats. However, only autologous ADSCs improved healing in acute burn wounds; allogenic ADSCs did not. This study further determined a superior location of using ADSCs injections to treat burn wounds including the injection site. Future studies will replicate the experiment in a larger and long-term scale burn wounds in higher mammalian models to facilitate ADSCs therapy in burn wound clinical practice.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29787581</pmid><doi>10.1371/journal.pone.0197744</doi><tpages>e0197744</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipocytes - cytology Animal models Animals Autografts Biology and Life Sciences Bone marrow Burns Burns - therapy Cancer therapies Care and treatment Cell Differentiation Cell Proliferation Cells, Cultured Diabetes Hospitals Injection Male Medical prognosis Medical research Medicine Medicine and Health Sciences Methods Plastic surgery Rats Rats, Wistar Skin Software Stem cell transplantation Stem Cell Transplantation - methods Stem cells Stem Cells - cytology Therapeutic applications Transplantation, Autologous Transplantation, Homologous Transplants & implants Wound Healing |
| title | Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing |
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