Comparison of two commercial carbapenemase gene confirmatory assays in multiresistant Enterobacteriaceae and Acinetobacter baumannii-complex
Multidrug-resistant Gram-negative bacilli (MDR-GNB) producing carbapenemases are increasing at an alarming speed. Rapid confirmation of carbapenemase type will be an important diagnostic step in clinical microbiology laboratories not only to reduce the risk of transmissions but also for optimising a...
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| creator | Rösner, Stephan Gehlweiler, Kevin Küsters, Uta Kolbert, Mathias Hübner, Kirsten Pfennigwerth, Niels Mack, Dietrich |
| description | Multidrug-resistant Gram-negative bacilli (MDR-GNB) producing carbapenemases are increasing at an alarming speed. Rapid confirmation of carbapenemase type will be an important diagnostic step in clinical microbiology laboratories not only to reduce the risk of transmissions but also for optimising antibiotic therapy in the future. We compared diagnostic reliability of two commercially available molecular assays (Check-Direct CPE vs. AID line probe assay) for detection and typing of carbapenemase genes in 80 well-characterized isolates of MDR-GNB. Respective strains were isolated in various clinical specimens at our clinical microbiology laboratory. The reference standard included confirmation of carbapenemase-production at the molecular level at the German National Reference Laboratory for Multidrug-resistant Gram-negative bacteria (Ruhr-University Bochum, Germany). 53 Enterobacteriaceae and 27 members of the A. baumannii-complex were used in this study. The tested assays appeared highly reliable to confirm carbapenemase-producing Enterobacteriaceae (CPE) with respective sensitivities of 97.7%, but are currently unsuitable for analysis of members of the A. baumannii-complex. Both assays are easy to perform and rapid tools for confirmation and typing of the most common carbapenemase genes in Enterobacteriaceae. Implementation should be possible for any clinical microbiology laboratory with Check-Direct CPE being easier to handle and having less technological requirements. |
| doi_str_mv | 10.1371/journal.pone.0197839 |
| format | Article |
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Rapid confirmation of carbapenemase type will be an important diagnostic step in clinical microbiology laboratories not only to reduce the risk of transmissions but also for optimising antibiotic therapy in the future. We compared diagnostic reliability of two commercially available molecular assays (Check-Direct CPE vs. AID line probe assay) for detection and typing of carbapenemase genes in 80 well-characterized isolates of MDR-GNB. Respective strains were isolated in various clinical specimens at our clinical microbiology laboratory. The reference standard included confirmation of carbapenemase-production at the molecular level at the German National Reference Laboratory for Multidrug-resistant Gram-negative bacteria (Ruhr-University Bochum, Germany). 53 Enterobacteriaceae and 27 members of the A. baumannii-complex were used in this study. The tested assays appeared highly reliable to confirm carbapenemase-producing Enterobacteriaceae (CPE) with respective sensitivities of 97.7%, but are currently unsuitable for analysis of members of the A. baumannii-complex. Both assays are easy to perform and rapid tools for confirmation and typing of the most common carbapenemase genes in Enterobacteriaceae. Implementation should be possible for any clinical microbiology laboratory with Check-Direct CPE being easier to handle and having less technological requirements.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0197839</identifier><identifier>PMID: 29782539</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acinetobacter baumannii ; Antibiotics ; Assaying ; Bacilli ; Beta lactamases ; Biology and Life Sciences ; Carbapenemase ; Clinical microbiology ; Comparative analysis ; Deoxyribonucleic acid ; Diagnostic systems ; DNA ; E coli ; Enterobacteriaceae ; Enzymes ; Escherichia coli ; Genes ; Genetic aspects ; Genetic testing ; Gram-negative bacilli ; Gram-negative bacteria ; Health aspects ; Infections ; Infectious diseases ; Laboratories ; Medicine and Health Sciences ; Methods ; Microbial drug resistance ; Microbiology ; Molecular chains ; Multidrug resistance ; People and places ; Research and Analysis Methods ; Risk reduction ; Sensitivity analysis ; Thermal cycling ; Typing</subject><ispartof>PloS one, 2018-05, Vol.13 (5), p.e0197839-e0197839</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Rösner et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Rapid confirmation of carbapenemase type will be an important diagnostic step in clinical microbiology laboratories not only to reduce the risk of transmissions but also for optimising antibiotic therapy in the future. We compared diagnostic reliability of two commercially available molecular assays (Check-Direct CPE vs. AID line probe assay) for detection and typing of carbapenemase genes in 80 well-characterized isolates of MDR-GNB. Respective strains were isolated in various clinical specimens at our clinical microbiology laboratory. The reference standard included confirmation of carbapenemase-production at the molecular level at the German National Reference Laboratory for Multidrug-resistant Gram-negative bacteria (Ruhr-University Bochum, Germany). 53 Enterobacteriaceae and 27 members of the A. baumannii-complex were used in this study. 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Implementation should be possible for any clinical microbiology laboratory with Check-Direct CPE being easier to handle and having less technological requirements.</description><subject>Acinetobacter baumannii</subject><subject>Antibiotics</subject><subject>Assaying</subject><subject>Bacilli</subject><subject>Beta lactamases</subject><subject>Biology and Life Sciences</subject><subject>Carbapenemase</subject><subject>Clinical microbiology</subject><subject>Comparative analysis</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic systems</subject><subject>DNA</subject><subject>E coli</subject><subject>Enterobacteriaceae</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic testing</subject><subject>Gram-negative bacilli</subject><subject>Gram-negative bacteria</subject><subject>Health aspects</subject><subject>Infections</subject><subject>Infectious 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of two commercial carbapenemase gene confirmatory assays in multiresistant Enterobacteriaceae and Acinetobacter baumannii-complex</title><author>Rösner, Stephan ; Gehlweiler, Kevin ; Küsters, Uta ; Kolbert, Mathias ; Hübner, Kirsten ; Pfennigwerth, Niels ; Mack, Dietrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-e1ba83bca6e60fef86671d2635d1cb6da7eb91ac8bbbeabd08184083fe38cfec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acinetobacter baumannii</topic><topic>Antibiotics</topic><topic>Assaying</topic><topic>Bacilli</topic><topic>Beta lactamases</topic><topic>Biology and Life Sciences</topic><topic>Carbapenemase</topic><topic>Clinical microbiology</topic><topic>Comparative analysis</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnostic systems</topic><topic>DNA</topic><topic>E 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Tomás</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of two commercial carbapenemase gene confirmatory assays in multiresistant Enterobacteriaceae and Acinetobacter baumannii-complex</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-05-21</date><risdate>2018</risdate><volume>13</volume><issue>5</issue><spage>e0197839</spage><epage>e0197839</epage><pages>e0197839-e0197839</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Multidrug-resistant Gram-negative bacilli (MDR-GNB) producing carbapenemases are increasing at an alarming speed. Rapid confirmation of carbapenemase type will be an important diagnostic step in clinical microbiology laboratories not only to reduce the risk of transmissions but also for optimising antibiotic therapy in the future. We compared diagnostic reliability of two commercially available molecular assays (Check-Direct CPE vs. AID line probe assay) for detection and typing of carbapenemase genes in 80 well-characterized isolates of MDR-GNB. Respective strains were isolated in various clinical specimens at our clinical microbiology laboratory. The reference standard included confirmation of carbapenemase-production at the molecular level at the German National Reference Laboratory for Multidrug-resistant Gram-negative bacteria (Ruhr-University Bochum, Germany). 53 Enterobacteriaceae and 27 members of the A. baumannii-complex were used in this study. The tested assays appeared highly reliable to confirm carbapenemase-producing Enterobacteriaceae (CPE) with respective sensitivities of 97.7%, but are currently unsuitable for analysis of members of the A. baumannii-complex. Both assays are easy to perform and rapid tools for confirmation and typing of the most common carbapenemase genes in Enterobacteriaceae. Implementation should be possible for any clinical microbiology laboratory with Check-Direct CPE being easier to handle and having less technological requirements.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29782539</pmid><doi>10.1371/journal.pone.0197839</doi><tpages>e0197839</tpages><orcidid>https://orcid.org/0000-0002-2879-2463</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acinetobacter baumannii Antibiotics Assaying Bacilli Beta lactamases Biology and Life Sciences Carbapenemase Clinical microbiology Comparative analysis Deoxyribonucleic acid Diagnostic systems DNA E coli Enterobacteriaceae Enzymes Escherichia coli Genes Genetic aspects Genetic testing Gram-negative bacilli Gram-negative bacteria Health aspects Infections Infectious diseases Laboratories Medicine and Health Sciences Methods Microbial drug resistance Microbiology Molecular chains Multidrug resistance People and places Research and Analysis Methods Risk reduction Sensitivity analysis Thermal cycling Typing |
| title | Comparison of two commercial carbapenemase gene confirmatory assays in multiresistant Enterobacteriaceae and Acinetobacter baumannii-complex |
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