Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics

Rabies is a fatal zoonotic disease that requires fast, accurate diagnosis to prevent disease in an exposed individual. The current gold standard for post-mortem diagnosis of human and animal rabies is the direct fluorescent antibody (DFA) test. While the DFA test has proven sensitive and reliable, i...

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Veröffentlicht in:	PloS one 2018-05, Vol.13 (5), p.e0197074-e0197074
Hauptverfasser:	Gigante, Crystal M, Dettinger, Lisa, Powell, James W, Seiders, Melanie, Condori, Rene Edgar Condori, Griesser, Richard, Okogi, Kenneth, Carlos, Maria, Pesko, Kendra, Breckenridge, Mike, Simon, Edson Michael M, Chu, Maria Yna Joyce V, Davis, April D, Brunt, Scott J, Orciari, Lillian, Yager, Pamela, Carson, William C, Hartloge, Claire, Saliki, Jeremiah T, Sanchez, Susan, Deldari, Mojgan, Hsieh, Kristina, Wadhwa, Ashutosh, Wilkins, Kimberly, Peredo, Veronica Yung, Rabideau, Patricia, Gruhn, Nina, Cadet, Rolain, Isloor, Shrikrishna, Nath, Sujith S, Joseph, Tomy, Gao, Jinxin, Wallace, Ryan, Reynolds, Mary, Olson, Victoria A, Li, Yu
Format:	Artikel
Sprache:	eng
Schlagworte:	60 APPLIED LIFE SCIENCES Animals Assaying Biology and life sciences Brain Brain research Decomposition Diagnosis Diagnostic systems Disease control Disease prevention Evaluation Fluorescence Genetic aspects Humans Infectious diseases lyssavirus Lyssavirus - genetics Medical laboratories Medicine and Health Sciences Methods Pathology People and places pilot studies Polymerase chain reaction Prevention Public health Rabies Rabies - diagnosis Rabies - genetics rabies virus Real time Real-Time Polymerase Chain Reaction - methods Reproducibility Research and analysis methods Reverse Transcriptase Polymerase Chain Reaction - methods reverse transcriptase-polymerase chain reaction Ribonucleic acid RNA RNA extraction RNA, Viral - genetics Sensitivity United States Viruses Zoonoses
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