Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish)
The extraction and purification of nucleic acids is the first step in most molecular biology analysis techniques. The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cann...
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| Veröffentlicht in: | PloS one 2013-10, Vol.8 (10), p.e74714-e74714 |
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| creator | El Alaoui, Moulay Abdelaziz Melloul, Marouane Alaoui Amine, Sanaâ Stambouli, Hamid El Bouri, Aziz Soulaymani, Abdelmajid El Fahime, Elmostafa |
| description | The extraction and purification of nucleic acids is the first step in most molecular biology analysis techniques. The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cannabis resin samples. To obtain highly purified nucleic acids from cannabis resin (Hashish) free from contaminants that cause inhibition of PCR reaction, we have tested two protocols: the CTAB protocol of Wagner and a CTAB protocol described by Somma (2004) adapted for difficult matrix. We obtained high quality genomic DNA from 8 cannabis resin seizures using the adapted protocol. DNA extracted by the Wagner CTAB protocol failed to give polymerase chain reaction (PCR) amplification of tetrahydrocannabinolic acid (THCA) synthase coding gene. However, the extracted DNA by the second protocol permits amplification of THCA synthase coding gene using different sets of primers as assessed by PCR. We describe here for the first time the possibility of DNA extraction from (Hashish) resin derived from Cannabis sativa. This allows the use of DNA molecular tests under special forensic circumstances. |
| doi_str_mv | 10.1371/journal.pone.0074714 |
| format | Article |
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The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cannabis resin samples. To obtain highly purified nucleic acids from cannabis resin (Hashish) free from contaminants that cause inhibition of PCR reaction, we have tested two protocols: the CTAB protocol of Wagner and a CTAB protocol described by Somma (2004) adapted for difficult matrix. We obtained high quality genomic DNA from 8 cannabis resin seizures using the adapted protocol. DNA extracted by the Wagner CTAB protocol failed to give polymerase chain reaction (PCR) amplification of tetrahydrocannabinolic acid (THCA) synthase coding gene. However, the extracted DNA by the second protocol permits amplification of THCA synthase coding gene using different sets of primers as assessed by PCR. We describe here for the first time the possibility of DNA extraction from (Hashish) resin derived from Cannabis sativa. This allows the use of DNA molecular tests under special forensic circumstances.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0074714</identifier><identifier>PMID: 24124454</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alcohol ; Amplification ; Analysis ; Cannabis ; Cannabis - genetics ; Cannabis sativa ; Contaminants ; Deoxyribonucleic acid ; DNA ; DNA, Plant - genetics ; Forensic engineering ; Forensic science ; Forensic sciences ; Genes ; Genetic research ; Genetically modified organisms ; International organizations ; Laboratories ; Leaves ; Marijuana ; Metabolites ; Methods ; Molecular biology ; Molecular chains ; Nucleic acids ; Polymerase Chain Reaction ; Polyphenols ; Primers ; Purification ; Researchers ; Seizing ; Seizures ; Typing</subject><ispartof>PloS one, 2013-10, Vol.8 (10), p.e74714-e74714</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 El Alaoui et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 El Alaoui et al 2013 El Alaoui et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-78774f64669c118ac3fe2723d867a0384f0c96dcc4f288d924e1f47d60770e633</citedby><cites>FETCH-LOGICAL-c758t-78774f64669c118ac3fe2723d867a0384f0c96dcc4f288d924e1f47d60770e633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790795/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790795/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24124454$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gilbert, Tom</contributor><creatorcontrib>El Alaoui, Moulay Abdelaziz</creatorcontrib><creatorcontrib>Melloul, Marouane</creatorcontrib><creatorcontrib>Alaoui Amine, Sanaâ</creatorcontrib><creatorcontrib>Stambouli, Hamid</creatorcontrib><creatorcontrib>El Bouri, Aziz</creatorcontrib><creatorcontrib>Soulaymani, Abdelmajid</creatorcontrib><creatorcontrib>El Fahime, Elmostafa</creatorcontrib><title>Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The extraction and purification of nucleic acids is the first step in most molecular biology analysis techniques. The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cannabis resin samples. To obtain highly purified nucleic acids from cannabis resin (Hashish) free from contaminants that cause inhibition of PCR reaction, we have tested two protocols: the CTAB protocol of Wagner and a CTAB protocol described by Somma (2004) adapted for difficult matrix. We obtained high quality genomic DNA from 8 cannabis resin seizures using the adapted protocol. DNA extracted by the Wagner CTAB protocol failed to give polymerase chain reaction (PCR) amplification of tetrahydrocannabinolic acid (THCA) synthase coding gene. However, the extracted DNA by the second protocol permits amplification of THCA synthase coding gene using different sets of primers as assessed by PCR. We describe here for the first time the possibility of DNA extraction from (Hashish) resin derived from Cannabis sativa. This allows the use of DNA molecular tests under special forensic circumstances.</description><subject>Alcohol</subject><subject>Amplification</subject><subject>Analysis</subject><subject>Cannabis</subject><subject>Cannabis - genetics</subject><subject>Cannabis sativa</subject><subject>Contaminants</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Plant - genetics</subject><subject>Forensic engineering</subject><subject>Forensic science</subject><subject>Forensic sciences</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genetically modified organisms</subject><subject>International organizations</subject><subject>Laboratories</subject><subject>Leaves</subject><subject>Marijuana</subject><subject>Metabolites</subject><subject>Methods</subject><subject>Molecular biology</subject><subject>Molecular chains</subject><subject>Nucleic acids</subject><subject>Polymerase Chain Reaction</subject><subject>Polyphenols</subject><subject>Primers</subject><subject>Purification</subject><subject>Researchers</subject><subject>Seizing</subject><subject>Seizures</subject><subject>Typing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl9r2zAUxc3YWLtu32BshsFoH5JJ1rUkvxRC262BbIX9exWyLMUKjpVK9mj76ac0bolHH4YwEte_e-x7dJLkLUZTTBj-tHK9b2Uz3bhWTxFiwDA8Sw5xQbIJzRB5vnc-SF6FsEIoJ5zSl8lBBjgDyOEwWVzcdF6qzro2dSat7bJOr3vZ2O42Pf82S4136zRoe6er9KvzTinZpvFpZWlD6nWwbXp8KUNtQ33yOnlhZBP0m2E_Sn59vvh5djlZXH2Zn80WE8Vy3k0YZwwMBUoLhTGXihidsYxUnDKJCAeDVEErpcBknFdFBhobYBVFjCFNCTlK3u90N40LYjAiiDhoVAFa5JGY74jKyZXYeLuW_lY4acV9wfmlkL6zqtGCGswqnhMos-iJJGUhaclKVTBTgcEqap0OX-vLta6UbqNjzUh0_Ka1tVi6P4KwArH7nzkeBLy77nXoxNoGpZtGttr1QWAAAhnlHCL64R_06ekGainjALY1bnuHW1ExA8YhyxljkZo-QcVV6bVVMTXGxvqo4WTUEJlO33RL2Ycg5j--_z979XvMftxjay2brg6u6behC2MQdqDyLgSvzaPJGIlt6B_cENvQiyH0se3d_gU9Nj2knPwF6VL6Hw</recordid><startdate>20131004</startdate><enddate>20131004</enddate><creator>El Alaoui, Moulay Abdelaziz</creator><creator>Melloul, Marouane</creator><creator>Alaoui Amine, Sanaâ</creator><creator>Stambouli, Hamid</creator><creator>El Bouri, Aziz</creator><creator>Soulaymani, Abdelmajid</creator><creator>El Fahime, Elmostafa</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131004</creationdate><title>Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish)</title><author>El Alaoui, Moulay Abdelaziz ; Melloul, Marouane ; Alaoui Amine, Sanaâ ; Stambouli, Hamid ; El Bouri, Aziz ; Soulaymani, Abdelmajid ; El Fahime, Elmostafa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-78774f64669c118ac3fe2723d867a0384f0c96dcc4f288d924e1f47d60770e633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alcohol</topic><topic>Amplification</topic><topic>Analysis</topic><topic>Cannabis</topic><topic>Cannabis - 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The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cannabis resin samples. To obtain highly purified nucleic acids from cannabis resin (Hashish) free from contaminants that cause inhibition of PCR reaction, we have tested two protocols: the CTAB protocol of Wagner and a CTAB protocol described by Somma (2004) adapted for difficult matrix. We obtained high quality genomic DNA from 8 cannabis resin seizures using the adapted protocol. DNA extracted by the Wagner CTAB protocol failed to give polymerase chain reaction (PCR) amplification of tetrahydrocannabinolic acid (THCA) synthase coding gene. However, the extracted DNA by the second protocol permits amplification of THCA synthase coding gene using different sets of primers as assessed by PCR. We describe here for the first time the possibility of DNA extraction from (Hashish) resin derived from Cannabis sativa. This allows the use of DNA molecular tests under special forensic circumstances.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24124454</pmid><doi>10.1371/journal.pone.0074714</doi><tpages>e74714</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alcohol Amplification Analysis Cannabis Cannabis - genetics Cannabis sativa Contaminants Deoxyribonucleic acid DNA DNA, Plant - genetics Forensic engineering Forensic science Forensic sciences Genes Genetic research Genetically modified organisms International organizations Laboratories Leaves Marijuana Metabolites Methods Molecular biology Molecular chains Nucleic acids Polymerase Chain Reaction Polyphenols Primers Purification Researchers Seizing Seizures Typing |
| title | Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish) |
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