Selection and evaluation of housekeeping genes as endogenous controls for quantification of mRNA transcripts in Theileria parva using quantitative real-time polymerase chain reaction (qPCR)

The reliability of any quantitative real-time polymerase chain reaction (qPCR) experiment can be seriously compromised by variations between samples as well as between PCR runs. This usually result from errors in sample quantification, especially with samples that are obtained from different individ...

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Veröffentlicht in:	PloS one 2018-05, Vol.13 (5), p.e0196715
Hauptverfasser:	Tsotetsi, Teboho N, Collins, Nicola E, Oosthuizen, Marinda C, Sibeko-Matjila, Kgomotso P
Format:	Artikel
Sprache:	eng
Schlagworte:	Actin Aldolase Analysis Animals Biology and Life Sciences Bovidae Buffaloes - genetics Cancer Cattle Cytochrome Cytochrome b Data processing Dehydrogenases Disease Evaluation Fructose Gene expression Gene Expression Regulation - genetics Genes Genes, Essential - genetics Glyceraldehyde-3-phosphate dehydrogenase Growth hormones Infections Medical research Medicine and Health Sciences Messenger RNA Methods Muscle proteins Parasites Polymerase chain reaction Proteins Protozoa Real time Real-Time Polymerase Chain Reaction - methods Reproducibility of Results Research and analysis methods RNA, Messenger - genetics rRNA 28S Theileria annulata Theileria parva Theileria parva - genetics Theileriasis - parasitology Ticks - genetics Transcription Transcription, Genetic - genetics Transfer RNA Tropical diseases Vectors (Biology)
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