Droplet-based microfluidic analysis and screening of single plant cells

Droplet-based microfluidics has been used to facilitate high-throughput analysis of individual prokaryote and mammalian cells. However, there is a scarcity of similar workflows applicable to rapid phenotyping of plant systems where phenotyping analyses typically are time-consuming and low-throughput...

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Veröffentlicht in:	PloS one 2018-05, Vol.13 (5), p.e0196810-e0196810
Hauptverfasser:	Yu, Ziyi, Boehm, Christian R, Hibberd, Julian M, Abell, Chris, Haseloff, Jim, Burgess, Steven J, Reyna-Llorens, Ivan
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium Agrobacterium tumefaciens - genetics Analysis Bacterial Proteins - analysis Bacterial Proteins - genetics Biology and Life Sciences Cell Separation - instrumentation Cell Separation - methods Cells (Biology) Deoxyribonucleic acid Dielectrophoresis DNA Drug Compounding Engineering and Technology Environmental conditions Equipment Design Gene expression Gene Expression Regulation, Plant Genes, Reporter Genetic engineering Genomes Genomics - methods High-Throughput Screening Assays - instrumentation High-Throughput Screening Assays - methods Hot Temperature Lab-On-A-Chip Devices Laboratories Luminescent Proteins - analysis Luminescent Proteins - genetics Mammalian cells Marchantia - chemistry Marchantia - cytology Marchantia - genetics Marchantia polymorpha Medical screening Medicine and Health Sciences Microfluidic Analytical Techniques - methods Microfluidics Microscopy, Fluorescence Phenotypes Phenotyping Physical Sciences Physiology Plant biology Plant cells Plant sciences Plants, Genetically Modified Prokaryotes Promoter Regions, Genetic Protoplasts Protoplasts - chemistry Single-Cell Analysis - instrumentation Single-Cell Analysis - methods Stochastic Processes Stochasticity Synthetic biology Transformation, Genetic
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