Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes
This work presents an alternative device for cancer screening in liquid biopsies. It combines a biomimetic film (i) with electrochemical detection (ii). The biomimetic film (i) was obtained by electro-polymerizing amine-substituted benzene rings around a CA 15-3 target. This protein target was previ...
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| description | This work presents an alternative device for cancer screening in liquid biopsies. It combines a biomimetic film (i) with electrochemical detection (ii). The biomimetic film (i) was obtained by electro-polymerizing amine-substituted benzene rings around a CA 15-3 target. This protein target was previously adsorbed on a gold (Au) support and incubated in charged monomers (4-Styrenesulfonate sodium and 3-Hydroxytyraminium chloride). The protein was further eliminated by enzymatic activity, leaving behind vacant sites for subsequent rebinding. Electrochemical detection (ii) was achieved on an Au working electrode, designed on commercial screen-printed electrodes. Raman spectroscopy, atomic force microscopy and ellipsometric readings were used to follow the chemical modification of the Au surface. The ability of the material to rebind CA15-3 was monitored by electrochemical techniques. The device displayed linear responses to CA15-3 ranging from 0.25 to 10.00 U/mL, with detection limits of 0.05 U/mL. Accurate results were obtained by applying the sensor to the analysis of CA15-3 in PBS buffer and in serum samples. This biosensing device displayed successful features for the detection of CA 15-3 and constitutes a promising tool for breast cancer screening procedures in point-of-care applications. Moreover, its scale-up seems feasible as it contains a plastic antibody assembled in situ, in less than 1 minute, and the analysis of serum takes less than 30 minutes. |
| doi_str_mv | 10.1371/journal.pone.0196656 |
| format | Article |
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It combines a biomimetic film (i) with electrochemical detection (ii). The biomimetic film (i) was obtained by electro-polymerizing amine-substituted benzene rings around a CA 15-3 target. This protein target was previously adsorbed on a gold (Au) support and incubated in charged monomers (4-Styrenesulfonate sodium and 3-Hydroxytyraminium chloride). The protein was further eliminated by enzymatic activity, leaving behind vacant sites for subsequent rebinding. Electrochemical detection (ii) was achieved on an Au working electrode, designed on commercial screen-printed electrodes. Raman spectroscopy, atomic force microscopy and ellipsometric readings were used to follow the chemical modification of the Au surface. The ability of the material to rebind CA15-3 was monitored by electrochemical techniques. The device displayed linear responses to CA15-3 ranging from 0.25 to 10.00 U/mL, with detection limits of 0.05 U/mL. Accurate results were obtained by applying the sensor to the analysis of CA15-3 in PBS buffer and in serum samples. This biosensing device displayed successful features for the detection of CA 15-3 and constitutes a promising tool for breast cancer screening procedures in point-of-care applications. Moreover, its scale-up seems feasible as it contains a plastic antibody assembled in situ, in less than 1 minute, and the analysis of serum takes less than 30 minutes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0196656</identifier><identifier>PMID: 29715330</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analytical chemistry ; Analytical methods ; Antibodies - chemistry ; Atomic beam spectroscopy ; Atomic force microscopy ; Benzene ; Bioengineering ; Biology and Life Sciences ; Biomarkers ; Biomimetics ; Biosensing Techniques - methods ; Biosensors ; Breast cancer ; Breast Neoplasms - diagnosis ; Cancer ; Cancer screening ; Chemical modification ; Detection limits ; Dopamine ; Early Detection of Cancer - methods ; Electrochemical analysis ; Electrochemical Techniques - methods ; Electrochemistry ; Electrodes ; Ellipsometry ; Engineering and Technology ; Engineering schools ; Enzymatic activity ; Female ; Films (Materials) ; Gold ; Gold - chemistry ; Humans ; Hydrocarbons ; Immunoassay ; Innovations ; Limit of Detection ; Liquid Biopsy - methods ; Medical equipment ; Microscopy ; Monomers ; Mucin-1 - chemistry ; o-Phenylenediamine ; Phenylenediamine ; Phenylenediamines - chemistry ; Physical chemistry ; Physical Sciences ; Plastics ; Point-of-Care Systems ; Polymers ; Polymers - chemistry ; Proteins ; Raman spectroscopy ; Screening ; Sensors ; Sodium ; Spectroscopy ; Technology application</subject><ispartof>PloS one, 2018-05, Vol.13 (5), p.e0196656-e0196656</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Gomes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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It combines a biomimetic film (i) with electrochemical detection (ii). The biomimetic film (i) was obtained by electro-polymerizing amine-substituted benzene rings around a CA 15-3 target. This protein target was previously adsorbed on a gold (Au) support and incubated in charged monomers (4-Styrenesulfonate sodium and 3-Hydroxytyraminium chloride). The protein was further eliminated by enzymatic activity, leaving behind vacant sites for subsequent rebinding. Electrochemical detection (ii) was achieved on an Au working electrode, designed on commercial screen-printed electrodes. Raman spectroscopy, atomic force microscopy and ellipsometric readings were used to follow the chemical modification of the Au surface. The ability of the material to rebind CA15-3 was monitored by electrochemical techniques. The device displayed linear responses to CA15-3 ranging from 0.25 to 10.00 U/mL, with detection limits of 0.05 U/mL. Accurate results were obtained by applying the sensor to the analysis of CA15-3 in PBS buffer and in serum samples. This biosensing device displayed successful features for the detection of CA 15-3 and constitutes a promising tool for breast cancer screening procedures in point-of-care applications. Moreover, its scale-up seems feasible as it contains a plastic antibody assembled in situ, in less than 1 minute, and the analysis of serum takes less than 30 minutes.</description><subject>Analytical chemistry</subject><subject>Analytical methods</subject><subject>Antibodies - chemistry</subject><subject>Atomic beam spectroscopy</subject><subject>Atomic force microscopy</subject><subject>Benzene</subject><subject>Bioengineering</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomimetics</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - diagnosis</subject><subject>Cancer</subject><subject>Cancer screening</subject><subject>Chemical modification</subject><subject>Detection limits</subject><subject>Dopamine</subject><subject>Early Detection of Cancer - methods</subject><subject>Electrochemical analysis</subject><subject>Electrochemical Techniques - 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chemistry</topic><topic>Atomic beam spectroscopy</topic><topic>Atomic force microscopy</topic><topic>Benzene</topic><topic>Bioengineering</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Biomimetics</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - diagnosis</topic><topic>Cancer</topic><topic>Cancer screening</topic><topic>Chemical modification</topic><topic>Detection limits</topic><topic>Dopamine</topic><topic>Early Detection of Cancer - methods</topic><topic>Electrochemical analysis</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Ellipsometry</topic><topic>Engineering and Technology</topic><topic>Engineering schools</topic><topic>Enzymatic activity</topic><topic>Female</topic><topic>Films (Materials)</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Humans</topic><topic>Hydrocarbons</topic><topic>Immunoassay</topic><topic>Innovations</topic><topic>Limit of Detection</topic><topic>Liquid Biopsy - 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It combines a biomimetic film (i) with electrochemical detection (ii). The biomimetic film (i) was obtained by electro-polymerizing amine-substituted benzene rings around a CA 15-3 target. This protein target was previously adsorbed on a gold (Au) support and incubated in charged monomers (4-Styrenesulfonate sodium and 3-Hydroxytyraminium chloride). The protein was further eliminated by enzymatic activity, leaving behind vacant sites for subsequent rebinding. Electrochemical detection (ii) was achieved on an Au working electrode, designed on commercial screen-printed electrodes. Raman spectroscopy, atomic force microscopy and ellipsometric readings were used to follow the chemical modification of the Au surface. The ability of the material to rebind CA15-3 was monitored by electrochemical techniques. The device displayed linear responses to CA15-3 ranging from 0.25 to 10.00 U/mL, with detection limits of 0.05 U/mL. Accurate results were obtained by applying the sensor to the analysis of CA15-3 in PBS buffer and in serum samples. This biosensing device displayed successful features for the detection of CA 15-3 and constitutes a promising tool for breast cancer screening procedures in point-of-care applications. Moreover, its scale-up seems feasible as it contains a plastic antibody assembled in situ, in less than 1 minute, and the analysis of serum takes less than 30 minutes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29715330</pmid><doi>10.1371/journal.pone.0196656</doi><orcidid>https://orcid.org/0000-0001-9936-7336</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analytical chemistry Analytical methods Antibodies - chemistry Atomic beam spectroscopy Atomic force microscopy Benzene Bioengineering Biology and Life Sciences Biomarkers Biomimetics Biosensing Techniques - methods Biosensors Breast cancer Breast Neoplasms - diagnosis Cancer Cancer screening Chemical modification Detection limits Dopamine Early Detection of Cancer - methods Electrochemical analysis Electrochemical Techniques - methods Electrochemistry Electrodes Ellipsometry Engineering and Technology Engineering schools Enzymatic activity Female Films (Materials) Gold Gold - chemistry Humans Hydrocarbons Immunoassay Innovations Limit of Detection Liquid Biopsy - methods Medical equipment Microscopy Monomers Mucin-1 - chemistry o-Phenylenediamine Phenylenediamine Phenylenediamines - chemistry Physical chemistry Physical Sciences Plastics Point-of-Care Systems Polymers Polymers - chemistry Proteins Raman spectroscopy Screening Sensors Sodium Spectroscopy Technology application |
| title | Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T14%3A46%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensing%20CA%2015-3%20in%20point-of-care%20by%20electropolymerizing%20O-phenylenediamine%20(oPDA)%20on%20Au-screen%20printed%20electrodes&rft.jtitle=PloS%20one&rft.au=Gomes,%20Rui%20S&rft.date=2018-05-01&rft.volume=13&rft.issue=5&rft.spage=e0196656&rft.epage=e0196656&rft.pages=e0196656-e0196656&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0196656&rft_dat=%3Cgale_plos_%3EA536938480%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2033293484&rft_id=info:pmid/29715330&rft_galeid=A536938480&rft_doaj_id=oai_doaj_org_article_cf07b0163f3e4566b3bbd98a761ed5e7&rfr_iscdi=true |