Airborne transmission of invasive fusariosis in patients with hematologic malignancies
From 2006 to 2013, an increasing incidence of fusariosis was observed in the hematologic patients of our University Hospital. We suspected of an environmental source, and the indoor hospital air was investigated as a potential source of the fungemia. Air samplings were performed in the hematology an...
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| Veröffentlicht in: | PloS one 2018-04, Vol.13 (4), p.e0196426 |
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| creator | Moretti, Maria Luiza Busso-Lopes, Ariane Fidelis Tararam, Cibele Aparecida Moraes, Renato Muraosa, Yasunori Mikami, Yuzuru Gonoi, Tohru Taguchi, Hideaki Lyra, Luzia Reichert-Lima, Franqueline Trabasso, Plínio de Hoog, Gerrit Sybren Al-Hatmi, Abdullah Mohammed Said Schreiber, Angelica Zaninelli Kamei, Katsuhiko |
| description | From 2006 to 2013, an increasing incidence of fusariosis was observed in the hematologic patients of our University Hospital. We suspected of an environmental source, and the indoor hospital air was investigated as a potential source of the fungemia. Air samplings were performed in the hematology and bone marrow transplant (BMT) wards using an air sampler with pre-defined air volumes. To study the molecular relationship among environmental and clinical isolates, 18 Fusarium spp. recovered from blood cultures were included in the study. DNA sequencing of a partial portion of TEF1α gene was performed for molecular identification. Molecular typing was carried out by multi-locus sequence typing (MLST) using a four-gene scheme: TEF1α, rDNA, RPB1 and RPB2. One hundred four isolates were recovered from the air of the hematology (n = 76) and the BMT (n = 28) wards. Fusarium isolates from the air were from five species complexes: Fusarium fujikuroi (FFSC, n = 56), Fusarium incarnatum-equiseti (FIESC, n = 24), Fusarium solani (FSSC, n = 13), Fusarium chlamydosporum (FCSC, n = 10), and Fusarium oxysporum (FOSC, n = 1). Fifteen Fusarium isolates recovered from blood belonged to FSSC, and three to FFSC. MLST identified the same sequence type (ST) in clinical and environmental isolates. ST1 was found in 5 isolates from blood and in 7 from the air, both identified as FSSC (Fusarium petroliphilum). STn1 was found in one isolate from blood and in one from the air, both identified as FFSC (Fusarium napiforme). F. napiforme was isolated from the air of the hospital room of the patient with fungemia due to F. napiforme. These findings suggested a possible clonal origin of the Fusarium spp. recovered from air and bloodcultures. In conclusion, our study found a diversity of Fusarium species in the air of our hospital, and a possible role of the air as source of systemic fusariosis in our immunocompromised patients. |
| doi_str_mv | 10.1371/journal.pone.0196426 |
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We suspected of an environmental source, and the indoor hospital air was investigated as a potential source of the fungemia. Air samplings were performed in the hematology and bone marrow transplant (BMT) wards using an air sampler with pre-defined air volumes. To study the molecular relationship among environmental and clinical isolates, 18 Fusarium spp. recovered from blood cultures were included in the study. DNA sequencing of a partial portion of TEF1α gene was performed for molecular identification. Molecular typing was carried out by multi-locus sequence typing (MLST) using a four-gene scheme: TEF1α, rDNA, RPB1 and RPB2. One hundred four isolates were recovered from the air of the hematology (n = 76) and the BMT (n = 28) wards. Fusarium isolates from the air were from five species complexes: Fusarium fujikuroi (FFSC, n = 56), Fusarium incarnatum-equiseti (FIESC, n = 24), Fusarium solani (FSSC, n = 13), Fusarium chlamydosporum (FCSC, n = 10), and Fusarium oxysporum (FOSC, n = 1). Fifteen Fusarium isolates recovered from blood belonged to FSSC, and three to FFSC. MLST identified the same sequence type (ST) in clinical and environmental isolates. ST1 was found in 5 isolates from blood and in 7 from the air, both identified as FSSC (Fusarium petroliphilum). STn1 was found in one isolate from blood and in one from the air, both identified as FFSC (Fusarium napiforme). F. napiforme was isolated from the air of the hospital room of the patient with fungemia due to F. napiforme. These findings suggested a possible clonal origin of the Fusarium spp. recovered from air and bloodcultures. In conclusion, our study found a diversity of Fusarium species in the air of our hospital, and a possible role of the air as source of systemic fusariosis in our immunocompromised patients.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0196426</identifier><identifier>PMID: 29698435</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Air sampling ; Aspergillus ; Biodiversity ; Biology and Life Sciences ; Blood ; Blood cancer ; Blood diseases ; Bone marrow ; Bone Marrow Transplantation ; Cancer ; Care and treatment ; Clinical isolates ; Computer and Information Sciences ; Deoxyribonucleic acid ; Disease control ; Disease transmission ; DNA ; DNA sequencing ; DNA, Fungal - chemistry ; DNA, Fungal - genetics ; DNA, Fungal - metabolism ; Ecosystems ; Epidemics ; Epidemiology ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungemia ; Fungi ; Fusariosis ; Fusariosis - complications ; Fusariosis - diagnosis ; Fusariosis - microbiology ; Fusarium ; Fusarium - classification ; Fusarium - genetics ; Fusarium - isolation & purification ; Fusarium fujikuroi ; Fusarium oxysporum ; Fusarium solani ; Gene sequencing ; Hematologic Neoplasms - complications ; Hematologic Neoplasms - pathology ; Hematologic Neoplasms - therapy ; Hematology ; Hospitals ; Humans ; Immunocompromised Host ; Immunocompromised hosts ; Indoor environments ; Infections ; Internal medicine ; Laboratories ; Medicine ; Medicine and Health Sciences ; Multilocus Sequence Typing ; Mycoses ; Pathology ; Patients ; Peptide Elongation Factor 1 - chemistry ; Peptide Elongation Factor 1 - genetics ; Peptide Elongation Factor 1 - metabolism ; Phylogeny ; Research and analysis methods ; Species diversity ; Transplants & implants</subject><ispartof>PloS one, 2018-04, Vol.13 (4), p.e0196426</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Moretti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Moretti et al 2018 Moretti et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-1b3a587dfb7ab13a951e19145b4d6fcc05cec71265d43debfabc0e92a463b4f13</citedby><cites>FETCH-LOGICAL-c692t-1b3a587dfb7ab13a951e19145b4d6fcc05cec71265d43debfabc0e92a463b4f13</cites><orcidid>0000-0002-6067-9049 ; 0000-0002-2280-5649 ; 0000-0002-0588-4859</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919535/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919535/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29698435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yun, Sung-Hwan</contributor><creatorcontrib>Moretti, Maria Luiza</creatorcontrib><creatorcontrib>Busso-Lopes, Ariane Fidelis</creatorcontrib><creatorcontrib>Tararam, Cibele Aparecida</creatorcontrib><creatorcontrib>Moraes, Renato</creatorcontrib><creatorcontrib>Muraosa, Yasunori</creatorcontrib><creatorcontrib>Mikami, Yuzuru</creatorcontrib><creatorcontrib>Gonoi, Tohru</creatorcontrib><creatorcontrib>Taguchi, Hideaki</creatorcontrib><creatorcontrib>Lyra, Luzia</creatorcontrib><creatorcontrib>Reichert-Lima, Franqueline</creatorcontrib><creatorcontrib>Trabasso, Plínio</creatorcontrib><creatorcontrib>de Hoog, Gerrit Sybren</creatorcontrib><creatorcontrib>Al-Hatmi, Abdullah Mohammed Said</creatorcontrib><creatorcontrib>Schreiber, Angelica Zaninelli</creatorcontrib><creatorcontrib>Kamei, Katsuhiko</creatorcontrib><title>Airborne transmission of invasive fusariosis in patients with hematologic malignancies</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>From 2006 to 2013, an increasing incidence of fusariosis was observed in the hematologic patients of our University Hospital. We suspected of an environmental source, and the indoor hospital air was investigated as a potential source of the fungemia. Air samplings were performed in the hematology and bone marrow transplant (BMT) wards using an air sampler with pre-defined air volumes. To study the molecular relationship among environmental and clinical isolates, 18 Fusarium spp. recovered from blood cultures were included in the study. DNA sequencing of a partial portion of TEF1α gene was performed for molecular identification. Molecular typing was carried out by multi-locus sequence typing (MLST) using a four-gene scheme: TEF1α, rDNA, RPB1 and RPB2. One hundred four isolates were recovered from the air of the hematology (n = 76) and the BMT (n = 28) wards. Fusarium isolates from the air were from five species complexes: Fusarium fujikuroi (FFSC, n = 56), Fusarium incarnatum-equiseti (FIESC, n = 24), Fusarium solani (FSSC, n = 13), Fusarium chlamydosporum (FCSC, n = 10), and Fusarium oxysporum (FOSC, n = 1). Fifteen Fusarium isolates recovered from blood belonged to FSSC, and three to FFSC. MLST identified the same sequence type (ST) in clinical and environmental isolates. ST1 was found in 5 isolates from blood and in 7 from the air, both identified as FSSC (Fusarium petroliphilum). STn1 was found in one isolate from blood and in one from the air, both identified as FFSC (Fusarium napiforme). F. napiforme was isolated from the air of the hospital room of the patient with fungemia due to F. napiforme. These findings suggested a possible clonal origin of the Fusarium spp. recovered from air and bloodcultures. In conclusion, our study found a diversity of Fusarium species in the air of our hospital, and a possible role of the air as source of systemic fusariosis in our immunocompromised patients.</description><subject>Air sampling</subject><subject>Aspergillus</subject><subject>Biodiversity</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood cancer</subject><subject>Blood diseases</subject><subject>Bone marrow</subject><subject>Bone Marrow Transplantation</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Clinical isolates</subject><subject>Computer and Information Sciences</subject><subject>Deoxyribonucleic acid</subject><subject>Disease control</subject><subject>Disease transmission</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Fungal - metabolism</subject><subject>Ecosystems</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungemia</subject><subject>Fungi</subject><subject>Fusariosis</subject><subject>Fusariosis - complications</subject><subject>Fusariosis - diagnosis</subject><subject>Fusariosis - microbiology</subject><subject>Fusarium</subject><subject>Fusarium - classification</subject><subject>Fusarium - genetics</subject><subject>Fusarium - isolation & purification</subject><subject>Fusarium fujikuroi</subject><subject>Fusarium oxysporum</subject><subject>Fusarium solani</subject><subject>Gene sequencing</subject><subject>Hematologic Neoplasms - complications</subject><subject>Hematologic Neoplasms - pathology</subject><subject>Hematologic Neoplasms - therapy</subject><subject>Hematology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunocompromised Host</subject><subject>Immunocompromised hosts</subject><subject>Indoor environments</subject><subject>Infections</subject><subject>Internal medicine</subject><subject>Laboratories</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Multilocus Sequence Typing</subject><subject>Mycoses</subject><subject>Pathology</subject><subject>Patients</subject><subject>Peptide Elongation Factor 1 - chemistry</subject><subject>Peptide Elongation Factor 1 - genetics</subject><subject>Peptide Elongation Factor 1 - metabolism</subject><subject>Phylogeny</subject><subject>Research and analysis methods</subject><subject>Species diversity</subject><subject>Transplants & implants</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl-L1DAUxYso7rr6DUQLguDDjE2Tps2LMCy6Diws-Gdfw02adDK0yZiko357MzvdZQoK0oeUm989uTk5WfYSFUuEa_R-60ZvoV_unFXLAjFKSvooO0cMlwtaFvjxyf9Z9iyEbVFUuKH0aXZWMsoagqvz7HZlvHDeqjx6sGEwIRhnc6dzY_cQzF7legzgjQsmpFq-g2iUjSH_aeIm36gBoutdZ2Q-QG86C1YaFZ5nTzT0Qb2Y1ovs-6eP3y4_L65vrtaXq-uFpKyMCyQwVE3dalGDQBhYhRRiiFSCtFRLWVRSyRqVtGoJbpXQIGShWAmEYkE0whfZ66PurneBT5YEnq6MCCJNUSRifSRaB1u-82YA_5s7MPyu4HzHwUcje8UVEbgtAZeS1UQBS0PVtKklxkI1Neik9WE6bRSDamXywUM_E53vWLPhndvziiFW4SoJvJkEvPsxqhD_MfJEdZCmMla7JCbT00i-qjDFuKTN4erLv1Dpa9VgZAqFNqk-a3g3a0hMVL9iB2MIfP31y_-zN7dz9u0Ju1HQx01w_RhTkMIcJEdQeheCV_rBOVTwQ6bv3eCHTPMp06nt1anrD033IcZ_AMst8-A</recordid><startdate>20180426</startdate><enddate>20180426</enddate><creator>Moretti, 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transmission of invasive fusariosis in patients with hematologic malignancies</title><author>Moretti, Maria Luiza ; Busso-Lopes, Ariane Fidelis ; Tararam, Cibele Aparecida ; Moraes, Renato ; Muraosa, Yasunori ; Mikami, Yuzuru ; Gonoi, Tohru ; Taguchi, Hideaki ; Lyra, Luzia ; Reichert-Lima, Franqueline ; Trabasso, Plínio ; de Hoog, Gerrit Sybren ; Al-Hatmi, Abdullah Mohammed Said ; Schreiber, Angelica Zaninelli ; Kamei, Katsuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-1b3a587dfb7ab13a951e19145b4d6fcc05cec71265d43debfabc0e92a463b4f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Air sampling</topic><topic>Aspergillus</topic><topic>Biodiversity</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood cancer</topic><topic>Blood diseases</topic><topic>Bone marrow</topic><topic>Bone Marrow Transplantation</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Clinical isolates</topic><topic>Computer and Information Sciences</topic><topic>Deoxyribonucleic acid</topic><topic>Disease control</topic><topic>Disease transmission</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Fungal - metabolism</topic><topic>Ecosystems</topic><topic>Epidemics</topic><topic>Epidemiology</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungemia</topic><topic>Fungi</topic><topic>Fusariosis</topic><topic>Fusariosis - complications</topic><topic>Fusariosis - diagnosis</topic><topic>Fusariosis - microbiology</topic><topic>Fusarium</topic><topic>Fusarium - classification</topic><topic>Fusarium - genetics</topic><topic>Fusarium - isolation & purification</topic><topic>Fusarium 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Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moretti, Maria Luiza</au><au>Busso-Lopes, Ariane Fidelis</au><au>Tararam, Cibele Aparecida</au><au>Moraes, Renato</au><au>Muraosa, Yasunori</au><au>Mikami, Yuzuru</au><au>Gonoi, Tohru</au><au>Taguchi, Hideaki</au><au>Lyra, Luzia</au><au>Reichert-Lima, Franqueline</au><au>Trabasso, Plínio</au><au>de Hoog, Gerrit Sybren</au><au>Al-Hatmi, Abdullah Mohammed Said</au><au>Schreiber, Angelica Zaninelli</au><au>Kamei, Katsuhiko</au><au>Yun, Sung-Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Airborne transmission of invasive fusariosis in patients with hematologic malignancies</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-04-26</date><risdate>2018</risdate><volume>13</volume><issue>4</issue><spage>e0196426</spage><pages>e0196426-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>From 2006 to 2013, an increasing incidence of fusariosis was observed in the hematologic patients of our University Hospital. We suspected of an environmental source, and the indoor hospital air was investigated as a potential source of the fungemia. Air samplings were performed in the hematology and bone marrow transplant (BMT) wards using an air sampler with pre-defined air volumes. To study the molecular relationship among environmental and clinical isolates, 18 Fusarium spp. recovered from blood cultures were included in the study. DNA sequencing of a partial portion of TEF1α gene was performed for molecular identification. Molecular typing was carried out by multi-locus sequence typing (MLST) using a four-gene scheme: TEF1α, rDNA, RPB1 and RPB2. One hundred four isolates were recovered from the air of the hematology (n = 76) and the BMT (n = 28) wards. Fusarium isolates from the air were from five species complexes: Fusarium fujikuroi (FFSC, n = 56), Fusarium incarnatum-equiseti (FIESC, n = 24), Fusarium solani (FSSC, n = 13), Fusarium chlamydosporum (FCSC, n = 10), and Fusarium oxysporum (FOSC, n = 1). Fifteen Fusarium isolates recovered from blood belonged to FSSC, and three to FFSC. MLST identified the same sequence type (ST) in clinical and environmental isolates. ST1 was found in 5 isolates from blood and in 7 from the air, both identified as FSSC (Fusarium petroliphilum). STn1 was found in one isolate from blood and in one from the air, both identified as FFSC (Fusarium napiforme). F. napiforme was isolated from the air of the hospital room of the patient with fungemia due to F. napiforme. These findings suggested a possible clonal origin of the Fusarium spp. recovered from air and bloodcultures. In conclusion, our study found a diversity of Fusarium species in the air of our hospital, and a possible role of the air as source of systemic fusariosis in our immunocompromised patients.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29698435</pmid><doi>10.1371/journal.pone.0196426</doi><tpages>e0196426</tpages><orcidid>https://orcid.org/0000-0002-6067-9049</orcidid><orcidid>https://orcid.org/0000-0002-2280-5649</orcidid><orcidid>https://orcid.org/0000-0002-0588-4859</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-04, Vol.13 (4), p.e0196426 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2031414800 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Air sampling Aspergillus Biodiversity Biology and Life Sciences Blood Blood cancer Blood diseases Bone marrow Bone Marrow Transplantation Cancer Care and treatment Clinical isolates Computer and Information Sciences Deoxyribonucleic acid Disease control Disease transmission DNA DNA sequencing DNA, Fungal - chemistry DNA, Fungal - genetics DNA, Fungal - metabolism Ecosystems Epidemics Epidemiology Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - metabolism Fungemia Fungi Fusariosis Fusariosis - complications Fusariosis - diagnosis Fusariosis - microbiology Fusarium Fusarium - classification Fusarium - genetics Fusarium - isolation & purification Fusarium fujikuroi Fusarium oxysporum Fusarium solani Gene sequencing Hematologic Neoplasms - complications Hematologic Neoplasms - pathology Hematologic Neoplasms - therapy Hematology Hospitals Humans Immunocompromised Host Immunocompromised hosts Indoor environments Infections Internal medicine Laboratories Medicine Medicine and Health Sciences Multilocus Sequence Typing Mycoses Pathology Patients Peptide Elongation Factor 1 - chemistry Peptide Elongation Factor 1 - genetics Peptide Elongation Factor 1 - metabolism Phylogeny Research and analysis methods Species diversity Transplants & implants |
| title | Airborne transmission of invasive fusariosis in patients with hematologic malignancies |
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