AHL-lactonase expression in three marine emerging pathogenic Vibrio spp. reduces virulence and mortality in brine shrimp (Artemia salina) and Manila clam (Venerupis philippinarum)
Bacterial infectious diseases produced by Vibrio are the main cause of economic losses in aquaculture. During recent years it has been shown that the expression of virulence genes in some Vibrio species is controlled by a population-density dependent gene-expression mechanism known as quorum sensing...
Gespeichert in:
| Veröffentlicht in: | PloS one 2018-04, Vol.13 (4), p.e0195176-e0195176 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0195176 |
|---|---|
| container_issue | 4 |
| container_start_page | e0195176 |
| container_title | PloS one |
| container_volume | 13 |
| creator | Torres, Marta Reina, José Carlos Fuentes-Monteverde, Juan Carlos Fernández, Gerardo Rodríguez, Jaime Jiménez, Carlos Llamas, Inmaculada |
| description | Bacterial infectious diseases produced by Vibrio are the main cause of economic losses in aquaculture. During recent years it has been shown that the expression of virulence genes in some Vibrio species is controlled by a population-density dependent gene-expression mechanism known as quorum sensing (QS), which is mediated by the diffusion of signal molecules such as N-acylhomoserine lactones (AHLs). QS disruption, especially the enzymatic degradation of signalling molecules, known as quorum quenching (QQ), is one of the novel therapeutic strategies for the treatment of bacterial infections. In this study, we present the detection of AHLs in 34 marine Vibrionaceae strains. Three aquaculture-related pathogenic Vibrio strains, V. mediterranei VibC-Oc-097, V. owensii VibC-Oc-106 and V. coralliilyticus VibC-Oc-193 were selected for further studies based on their virulence and high production of AHLs. This is the first report where the signal molecules have been characterized in these emerging marine pathogens and correlated to the expression of virulence factors. Moreover, the results of AHL inactivation in the three selected strains have been confirmed in vivo against brine shrimps (Artemia salina) and Manila clams (Venerupis philippinarum). This research contributes to the development of future therapies based on AHL disruption, the most promising alternatives for fighting infectious diseases in aquaculture. |
| doi_str_mv | 10.1371/journal.pone.0195176 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_2026391777</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_88ad1b03c7eb45058bcbfa2e8c5d5f32</doaj_id><sourcerecordid>2026391777</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-bbe34579bbddad331093b097164cfdfbcb9b47ed1806496ba8fa8e649e6219773</originalsourceid><addsrcrecordid>eNptkk1v1DAQhiMEoqXwDxBY4rI97GLHiZNckFZVoZUWcYFerbEz2fXKsY2dVPR38Qeb_WjVIk4eed555kNvlr1ndMF4xT5v_Rgd2EXwDheUNSWrxIvslDU8n4uc8pdP4pPsTUpbSkteC_E6O8kbIYqGFafZ3-XVam5BD95BQoJ_QsSUjHfEODJsIiLpIRo3pXqMa-PWJMCw8Wt0RpMbo6LxJIWwIBHbUWMityaOFp1GAq4lvY8DWDPc7XhqD0qbaPpAZss4YG-ApCnv4Hwv_w7OWCDaQk9mN-gwjsEkEjbGmhAmWRz787fZqw5swnfH9yz79fXy58XVfPXj2_XFcjXXZS6GuVLIi7JqlGpbaDlntOGKNhUThe7aTmnVqKLCltV0OoZQUHdQ4xSiyFlTVfws-3jgBuuTPN47yZzmgjes2iuuD4rWw1aGaS-Id9KDkfsPH9cS4mC0RVnX0DJFua5QFSUt66l_BznWumzLjucT68ux26h6bDW6IYJ9Bn2ecWYj1_5Wlg3loqATYHYERP97xDTI3iSN1oJDP-7nrmjF6qKepJ_-kf5_u-Kg0tGnFLF7HIZRufPgQ5XceVAePTiVfXi6yGPRg-n4PQ4B3wI</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2026391777</pqid></control><display><type>article</type><title>AHL-lactonase expression in three marine emerging pathogenic Vibrio spp. reduces virulence and mortality in brine shrimp (Artemia salina) and Manila clam (Venerupis philippinarum)</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Torres, Marta ; Reina, José Carlos ; Fuentes-Monteverde, Juan Carlos ; Fernández, Gerardo ; Rodríguez, Jaime ; Jiménez, Carlos ; Llamas, Inmaculada</creator><contributor>Leoni, Livia</contributor><creatorcontrib>Torres, Marta ; Reina, José Carlos ; Fuentes-Monteverde, Juan Carlos ; Fernández, Gerardo ; Rodríguez, Jaime ; Jiménez, Carlos ; Llamas, Inmaculada ; Leoni, Livia</creatorcontrib><description>Bacterial infectious diseases produced by Vibrio are the main cause of economic losses in aquaculture. During recent years it has been shown that the expression of virulence genes in some Vibrio species is controlled by a population-density dependent gene-expression mechanism known as quorum sensing (QS), which is mediated by the diffusion of signal molecules such as N-acylhomoserine lactones (AHLs). QS disruption, especially the enzymatic degradation of signalling molecules, known as quorum quenching (QQ), is one of the novel therapeutic strategies for the treatment of bacterial infections. In this study, we present the detection of AHLs in 34 marine Vibrionaceae strains. Three aquaculture-related pathogenic Vibrio strains, V. mediterranei VibC-Oc-097, V. owensii VibC-Oc-106 and V. coralliilyticus VibC-Oc-193 were selected for further studies based on their virulence and high production of AHLs. This is the first report where the signal molecules have been characterized in these emerging marine pathogens and correlated to the expression of virulence factors. Moreover, the results of AHL inactivation in the three selected strains have been confirmed in vivo against brine shrimps (Artemia salina) and Manila clams (Venerupis philippinarum). This research contributes to the development of future therapies based on AHL disruption, the most promising alternatives for fighting infectious diseases in aquaculture.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0195176</identifier><identifier>PMID: 29664914</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acyl-Butyrolactones - metabolism ; Animals ; Aquaculture ; Artemia ; Artemia - microbiology ; Artemia salina ; Bacteria ; Biodegradation ; Biofilms ; Biology and Life Sciences ; Biomedical research ; Bivalvia - microbiology ; Brines ; Carboxylic Ester Hydrolases - genetics ; Carboxylic Ester Hydrolases - metabolism ; Communicable Diseases, Emerging - genetics ; Communicable Diseases, Emerging - metabolism ; Communicable Diseases, Emerging - microbiology ; Communicable Diseases, Emerging - veterinary ; Communication ; Deactivation ; Economic impact ; Engineering and Technology ; Enzymes ; Firmicutes ; Gene Deletion ; Gene expression ; Gene Expression Regulation, Bacterial - physiology ; Gene Expression Regulation, Enzymologic - physiology ; Genomes ; Inactivation ; Infectious diseases ; Lactones ; Medicine and Health Sciences ; Mollusca ; Mollusks ; Mortality ; Organisms, Genetically Modified ; Pathogens ; Pharmacy ; Population density ; Proteobacteria ; Quorum sensing ; Quorum Sensing - genetics ; Research and Analysis Methods ; Saline water ; Shrimps ; Signaling ; Strains (organisms) ; Venerupis philippinarum ; Vibrio ; Vibrio - genetics ; Vibrio - pathogenicity ; Vibrio Infections - genetics ; Vibrio Infections - metabolism ; Vibrio Infections - veterinary ; Virulence ; Virulence - genetics ; Virulence factors ; Waterborne diseases</subject><ispartof>PloS one, 2018-04, Vol.13 (4), p.e0195176-e0195176</ispartof><rights>2018 Torres et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Torres et al 2018 Torres et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-bbe34579bbddad331093b097164cfdfbcb9b47ed1806496ba8fa8e649e6219773</citedby><cites>FETCH-LOGICAL-c526t-bbe34579bbddad331093b097164cfdfbcb9b47ed1806496ba8fa8e649e6219773</cites><orcidid>0000-0001-6333-5033</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903640/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903640/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29664914$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Leoni, Livia</contributor><creatorcontrib>Torres, Marta</creatorcontrib><creatorcontrib>Reina, José Carlos</creatorcontrib><creatorcontrib>Fuentes-Monteverde, Juan Carlos</creatorcontrib><creatorcontrib>Fernández, Gerardo</creatorcontrib><creatorcontrib>Rodríguez, Jaime</creatorcontrib><creatorcontrib>Jiménez, Carlos</creatorcontrib><creatorcontrib>Llamas, Inmaculada</creatorcontrib><title>AHL-lactonase expression in three marine emerging pathogenic Vibrio spp. reduces virulence and mortality in brine shrimp (Artemia salina) and Manila clam (Venerupis philippinarum)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bacterial infectious diseases produced by Vibrio are the main cause of economic losses in aquaculture. During recent years it has been shown that the expression of virulence genes in some Vibrio species is controlled by a population-density dependent gene-expression mechanism known as quorum sensing (QS), which is mediated by the diffusion of signal molecules such as N-acylhomoserine lactones (AHLs). QS disruption, especially the enzymatic degradation of signalling molecules, known as quorum quenching (QQ), is one of the novel therapeutic strategies for the treatment of bacterial infections. In this study, we present the detection of AHLs in 34 marine Vibrionaceae strains. Three aquaculture-related pathogenic Vibrio strains, V. mediterranei VibC-Oc-097, V. owensii VibC-Oc-106 and V. coralliilyticus VibC-Oc-193 were selected for further studies based on their virulence and high production of AHLs. This is the first report where the signal molecules have been characterized in these emerging marine pathogens and correlated to the expression of virulence factors. Moreover, the results of AHL inactivation in the three selected strains have been confirmed in vivo against brine shrimps (Artemia salina) and Manila clams (Venerupis philippinarum). This research contributes to the development of future therapies based on AHL disruption, the most promising alternatives for fighting infectious diseases in aquaculture.</description><subject>Acyl-Butyrolactones - metabolism</subject><subject>Animals</subject><subject>Aquaculture</subject><subject>Artemia</subject><subject>Artemia - microbiology</subject><subject>Artemia salina</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Biofilms</subject><subject>Biology and Life Sciences</subject><subject>Biomedical research</subject><subject>Bivalvia - microbiology</subject><subject>Brines</subject><subject>Carboxylic Ester Hydrolases - genetics</subject><subject>Carboxylic Ester Hydrolases - metabolism</subject><subject>Communicable Diseases, Emerging - genetics</subject><subject>Communicable Diseases, Emerging - metabolism</subject><subject>Communicable Diseases, Emerging - microbiology</subject><subject>Communicable Diseases, Emerging - veterinary</subject><subject>Communication</subject><subject>Deactivation</subject><subject>Economic impact</subject><subject>Engineering and Technology</subject><subject>Enzymes</subject><subject>Firmicutes</subject><subject>Gene Deletion</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial - physiology</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Genomes</subject><subject>Inactivation</subject><subject>Infectious diseases</subject><subject>Lactones</subject><subject>Medicine and Health Sciences</subject><subject>Mollusca</subject><subject>Mollusks</subject><subject>Mortality</subject><subject>Organisms, Genetically Modified</subject><subject>Pathogens</subject><subject>Pharmacy</subject><subject>Population density</subject><subject>Proteobacteria</subject><subject>Quorum sensing</subject><subject>Quorum Sensing - genetics</subject><subject>Research and Analysis Methods</subject><subject>Saline water</subject><subject>Shrimps</subject><subject>Signaling</subject><subject>Strains (organisms)</subject><subject>Venerupis philippinarum</subject><subject>Vibrio</subject><subject>Vibrio - genetics</subject><subject>Vibrio - pathogenicity</subject><subject>Vibrio Infections - genetics</subject><subject>Vibrio Infections - metabolism</subject><subject>Vibrio Infections - veterinary</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><subject>Virulence factors</subject><subject>Waterborne diseases</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptkk1v1DAQhiMEoqXwDxBY4rI97GLHiZNckFZVoZUWcYFerbEz2fXKsY2dVPR38Qeb_WjVIk4eed555kNvlr1ndMF4xT5v_Rgd2EXwDheUNSWrxIvslDU8n4uc8pdP4pPsTUpbSkteC_E6O8kbIYqGFafZ3-XVam5BD95BQoJ_QsSUjHfEODJsIiLpIRo3pXqMa-PWJMCw8Wt0RpMbo6LxJIWwIBHbUWMityaOFp1GAq4lvY8DWDPc7XhqD0qbaPpAZss4YG-ApCnv4Hwv_w7OWCDaQk9mN-gwjsEkEjbGmhAmWRz787fZqw5swnfH9yz79fXy58XVfPXj2_XFcjXXZS6GuVLIi7JqlGpbaDlntOGKNhUThe7aTmnVqKLCltV0OoZQUHdQ4xSiyFlTVfws-3jgBuuTPN47yZzmgjes2iuuD4rWw1aGaS-Id9KDkfsPH9cS4mC0RVnX0DJFua5QFSUt66l_BznWumzLjucT68ux26h6bDW6IYJ9Bn2ecWYj1_5Wlg3loqATYHYERP97xDTI3iSN1oJDP-7nrmjF6qKepJ_-kf5_u-Kg0tGnFLF7HIZRufPgQ5XceVAePTiVfXi6yGPRg-n4PQ4B3wI</recordid><startdate>20180417</startdate><enddate>20180417</enddate><creator>Torres, Marta</creator><creator>Reina, José Carlos</creator><creator>Fuentes-Monteverde, Juan Carlos</creator><creator>Fernández, Gerardo</creator><creator>Rodríguez, Jaime</creator><creator>Jiménez, Carlos</creator><creator>Llamas, Inmaculada</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6333-5033</orcidid></search><sort><creationdate>20180417</creationdate><title>AHL-lactonase expression in three marine emerging pathogenic Vibrio spp. reduces virulence and mortality in brine shrimp (Artemia salina) and Manila clam (Venerupis philippinarum)</title><author>Torres, Marta ; Reina, José Carlos ; Fuentes-Monteverde, Juan Carlos ; Fernández, Gerardo ; Rodríguez, Jaime ; Jiménez, Carlos ; Llamas, Inmaculada</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-bbe34579bbddad331093b097164cfdfbcb9b47ed1806496ba8fa8e649e6219773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acyl-Butyrolactones - metabolism</topic><topic>Animals</topic><topic>Aquaculture</topic><topic>Artemia</topic><topic>Artemia - microbiology</topic><topic>Artemia salina</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>Biofilms</topic><topic>Biology and Life Sciences</topic><topic>Biomedical research</topic><topic>Bivalvia - microbiology</topic><topic>Brines</topic><topic>Carboxylic Ester Hydrolases - genetics</topic><topic>Carboxylic Ester Hydrolases - metabolism</topic><topic>Communicable Diseases, Emerging - genetics</topic><topic>Communicable Diseases, Emerging - metabolism</topic><topic>Communicable Diseases, Emerging - microbiology</topic><topic>Communicable Diseases, Emerging - veterinary</topic><topic>Communication</topic><topic>Deactivation</topic><topic>Economic impact</topic><topic>Engineering and Technology</topic><topic>Enzymes</topic><topic>Firmicutes</topic><topic>Gene Deletion</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial - physiology</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Genomes</topic><topic>Inactivation</topic><topic>Infectious diseases</topic><topic>Lactones</topic><topic>Medicine and Health Sciences</topic><topic>Mollusca</topic><topic>Mollusks</topic><topic>Mortality</topic><topic>Organisms, Genetically Modified</topic><topic>Pathogens</topic><topic>Pharmacy</topic><topic>Population density</topic><topic>Proteobacteria</topic><topic>Quorum sensing</topic><topic>Quorum Sensing - genetics</topic><topic>Research and Analysis Methods</topic><topic>Saline water</topic><topic>Shrimps</topic><topic>Signaling</topic><topic>Strains (organisms)</topic><topic>Venerupis philippinarum</topic><topic>Vibrio</topic><topic>Vibrio - genetics</topic><topic>Vibrio - pathogenicity</topic><topic>Vibrio Infections - genetics</topic><topic>Vibrio Infections - metabolism</topic><topic>Vibrio Infections - veterinary</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><topic>Virulence factors</topic><topic>Waterborne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torres, Marta</creatorcontrib><creatorcontrib>Reina, José Carlos</creatorcontrib><creatorcontrib>Fuentes-Monteverde, Juan Carlos</creatorcontrib><creatorcontrib>Fernández, Gerardo</creatorcontrib><creatorcontrib>Rodríguez, Jaime</creatorcontrib><creatorcontrib>Jiménez, Carlos</creatorcontrib><creatorcontrib>Llamas, Inmaculada</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torres, Marta</au><au>Reina, José Carlos</au><au>Fuentes-Monteverde, Juan Carlos</au><au>Fernández, Gerardo</au><au>Rodríguez, Jaime</au><au>Jiménez, Carlos</au><au>Llamas, Inmaculada</au><au>Leoni, Livia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AHL-lactonase expression in three marine emerging pathogenic Vibrio spp. reduces virulence and mortality in brine shrimp (Artemia salina) and Manila clam (Venerupis philippinarum)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-04-17</date><risdate>2018</risdate><volume>13</volume><issue>4</issue><spage>e0195176</spage><epage>e0195176</epage><pages>e0195176-e0195176</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bacterial infectious diseases produced by Vibrio are the main cause of economic losses in aquaculture. During recent years it has been shown that the expression of virulence genes in some Vibrio species is controlled by a population-density dependent gene-expression mechanism known as quorum sensing (QS), which is mediated by the diffusion of signal molecules such as N-acylhomoserine lactones (AHLs). QS disruption, especially the enzymatic degradation of signalling molecules, known as quorum quenching (QQ), is one of the novel therapeutic strategies for the treatment of bacterial infections. In this study, we present the detection of AHLs in 34 marine Vibrionaceae strains. Three aquaculture-related pathogenic Vibrio strains, V. mediterranei VibC-Oc-097, V. owensii VibC-Oc-106 and V. coralliilyticus VibC-Oc-193 were selected for further studies based on their virulence and high production of AHLs. This is the first report where the signal molecules have been characterized in these emerging marine pathogens and correlated to the expression of virulence factors. Moreover, the results of AHL inactivation in the three selected strains have been confirmed in vivo against brine shrimps (Artemia salina) and Manila clams (Venerupis philippinarum). This research contributes to the development of future therapies based on AHL disruption, the most promising alternatives for fighting infectious diseases in aquaculture.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29664914</pmid><doi>10.1371/journal.pone.0195176</doi><orcidid>https://orcid.org/0000-0001-6333-5033</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-04, Vol.13 (4), p.e0195176-e0195176 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2026391777 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acyl-Butyrolactones - metabolism Animals Aquaculture Artemia Artemia - microbiology Artemia salina Bacteria Biodegradation Biofilms Biology and Life Sciences Biomedical research Bivalvia - microbiology Brines Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - metabolism Communicable Diseases, Emerging - genetics Communicable Diseases, Emerging - metabolism Communicable Diseases, Emerging - microbiology Communicable Diseases, Emerging - veterinary Communication Deactivation Economic impact Engineering and Technology Enzymes Firmicutes Gene Deletion Gene expression Gene Expression Regulation, Bacterial - physiology Gene Expression Regulation, Enzymologic - physiology Genomes Inactivation Infectious diseases Lactones Medicine and Health Sciences Mollusca Mollusks Mortality Organisms, Genetically Modified Pathogens Pharmacy Population density Proteobacteria Quorum sensing Quorum Sensing - genetics Research and Analysis Methods Saline water Shrimps Signaling Strains (organisms) Venerupis philippinarum Vibrio Vibrio - genetics Vibrio - pathogenicity Vibrio Infections - genetics Vibrio Infections - metabolism Vibrio Infections - veterinary Virulence Virulence - genetics Virulence factors Waterborne diseases |
| title | AHL-lactonase expression in three marine emerging pathogenic Vibrio spp. reduces virulence and mortality in brine shrimp (Artemia salina) and Manila clam (Venerupis philippinarum) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T12%3A04%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=AHL-lactonase%20expression%20in%20three%20marine%20emerging%20pathogenic%20Vibrio%20spp.%20reduces%20virulence%20and%20mortality%20in%20brine%20shrimp%20(Artemia%20salina)%20and%20Manila%20clam%20(Venerupis%20philippinarum)&rft.jtitle=PloS%20one&rft.au=Torres,%20Marta&rft.date=2018-04-17&rft.volume=13&rft.issue=4&rft.spage=e0195176&rft.epage=e0195176&rft.pages=e0195176-e0195176&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0195176&rft_dat=%3Cproquest_plos_%3E2026391777%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2026391777&rft_id=info:pmid/29664914&rft_doaj_id=oai_doaj_org_article_88ad1b03c7eb45058bcbfa2e8c5d5f32&rfr_iscdi=true |