The non-canonical Notch signaling is essential for the control of fertility in Aedes aegypti

The Notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. It plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embry...

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Veröffentlicht in:PLoS neglected tropical diseases 2018-03, Vol.12 (3), p.e0006307-e0006307
Hauptverfasser: Chang, Chia-Hao, Liu, Yu-Ting, Weng, Shih-Che, Chen, I-Yi, Tsao, Po-Nien, Shiao, Shin-Hong
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container_title PLoS neglected tropical diseases
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creator Chang, Chia-Hao
Liu, Yu-Ting
Weng, Shih-Che
Chen, I-Yi
Tsao, Po-Nien
Shiao, Shin-Hong
description The Notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. It plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. However, functions of Notch signaling in Aedes aegypti, the major mosquito vector for dengue, are largely unknown. In this study, we identified a unique feature of A. aegypti Notch (AaNotch) in the control of the sterile-like phenotype in female mosquitoes. Silencing AaNotch with a reverse genetic approach significantly reduced the fecundity and fertility of the mosquito. Silencing AaNotch also resulted in the prevention of micropyle formation, which led to impaired fertilization. In addition, JNK phosphorylation (a signaling molecule in the non-canonical Notch signaling pathway) was inhibited in the absence of AaNotch. Furthermore, treatment with a JNK inhibitor in the mosquito resulted in impaired fecundity and fertility. Taken together, our results demonstrate that non-canonical Notch signaling is essential for controlling fertility in the A. aegypti mosquito.
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It plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. However, functions of Notch signaling in Aedes aegypti, the major mosquito vector for dengue, are largely unknown. In this study, we identified a unique feature of A. aegypti Notch (AaNotch) in the control of the sterile-like phenotype in female mosquitoes. Silencing AaNotch with a reverse genetic approach significantly reduced the fecundity and fertility of the mosquito. Silencing AaNotch also resulted in the prevention of micropyle formation, which led to impaired fertilization. In addition, JNK phosphorylation (a signaling molecule in the non-canonical Notch signaling pathway) was inhibited in the absence of AaNotch. Furthermore, treatment with a JNK inhibitor in the mosquito resulted in impaired fecundity and fertility. 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subjects Adenosine triphosphatase
Aedes aegypti
Amino acids
Aquatic insects
Biological fertilization
Biology and Life Sciences
Cell proliferation
Cellular signal transduction
Control
Culicidae
Dengue
Dengue fever
Drosophila
Embryogenesis
Fecundity
Fertility
Fertilization
Genetic aspects
Human diseases
Insecticides
Insects
JNK protein
Medicine and Health Sciences
Mosquitoes
Notch protein
Parasitology
Phenotypes
Phosphorylation
Physiological aspects
Product development
Proliferation
Research and Analysis Methods
Signal transduction
Signaling
Stem cells
Tropical diseases
Vaccines
Vector-borne diseases
Wolbachia
Yellow fever mosquito
title The non-canonical Notch signaling is essential for the control of fertility in Aedes aegypti
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