DNA barcoding reveals seasonal shifts in diet and consumption of deep-sea fishes in wedge-tailed shearwaters
The foraging ecology of pelagic seabirds is difficult to characterize because of their large foraging areas. In the face of this difficulty, DNA metabarcoding may be a useful approach to analyze diet compositions and foraging behaviors. Using this approach, we investigated the diet composition and i...
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| creator | Komura, Taketo Ando, Haruko Horikoshi, Kazuo Suzuki, Hajime Isagi, Yuji |
| description | The foraging ecology of pelagic seabirds is difficult to characterize because of their large foraging areas. In the face of this difficulty, DNA metabarcoding may be a useful approach to analyze diet compositions and foraging behaviors. Using this approach, we investigated the diet composition and its seasonal variation of a common seabird species on the Ogasawara Islands, Japan: the wedge-tailed shearwater Ardenna pacifica. We collected fecal samples during the prebreeding (N = 73) and rearing (N = 96) periods. The diet composition of wedge-tailed shearwater was analyzed by Ion Torrent sequencing using two universal polymerase chain reaction primers for the 12S and 16S mitochondrial DNA regions that targeted vertebrates and mollusks, respectively. The results of a BLAST search of obtained sequences detected 31 and 1 vertebrate and mollusk taxa, respectively. The results of the diet composition analysis showed that wedge-tailed shearwaters frequently consumed deep-sea fishes throughout the sampling season, indicating the importance of these fishes as a stable food resource. However, there was a marked seasonal shift in diet, which may reflect seasonal changes in food resource availability and wedge-tailed shearwater foraging behavior. The collected data regarding the shearwater diet may be useful for in situ conservation efforts. Future research that combines DNA metabarcoding with other tools, such as data logging, may provide further insight into the foraging ecology of pelagic seabirds. |
| doi_str_mv | 10.1371/journal.pone.0195385 |
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In the face of this difficulty, DNA metabarcoding may be a useful approach to analyze diet compositions and foraging behaviors. Using this approach, we investigated the diet composition and its seasonal variation of a common seabird species on the Ogasawara Islands, Japan: the wedge-tailed shearwater Ardenna pacifica. We collected fecal samples during the prebreeding (N = 73) and rearing (N = 96) periods. The diet composition of wedge-tailed shearwater was analyzed by Ion Torrent sequencing using two universal polymerase chain reaction primers for the 12S and 16S mitochondrial DNA regions that targeted vertebrates and mollusks, respectively. The results of a BLAST search of obtained sequences detected 31 and 1 vertebrate and mollusk taxa, respectively. The results of the diet composition analysis showed that wedge-tailed shearwaters frequently consumed deep-sea fishes throughout the sampling season, indicating the importance of these fishes as a stable food resource. However, there was a marked seasonal shift in diet, which may reflect seasonal changes in food resource availability and wedge-tailed shearwater foraging behavior. The collected data regarding the shearwater diet may be useful for in situ conservation efforts. Future research that combines DNA metabarcoding with other tools, such as data logging, may provide further insight into the foraging ecology of pelagic seabirds.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0195385</identifier><identifier>PMID: 29630670</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal behavior ; Animals ; Aquatic birds ; Aquatic Organisms - genetics ; Behavior ; Biological research ; Biology and Life Sciences ; Birds - physiology ; Breeding ; Conservation ; Data logging ; Deep sea ; Deoxyribonucleic acid ; Depth indicators ; Diet ; DNA ; DNA barcoding ; DNA Barcoding, Taxonomic ; Earth Sciences ; Ecology ; Ecology and Environmental Sciences ; Ecosystem ; Ecosystems ; Exocoetidae ; Feces ; Feeding Behavior ; Female ; Fisheries ; Fishes - genetics ; Fishing ; Food ; Food and nutrition ; Food availability ; Food Chain ; Foraging behavior ; Gene sequencing ; Hoarding (Animal feeding behavior) ; Islands ; Japan ; Male ; Medicine and Health Sciences ; Methods ; Mitochondrial DNA ; Mollusca - genetics ; Mollusks ; Oceans ; Polymerase chain reaction ; Population ; Predatory Behavior ; Primers ; Procellariidae ; Puffinus yelkouan ; Research and Analysis Methods ; Resource availability ; Seabirds ; Seasonal variations ; Seasons ; Shearwaters ; Studies ; Vertebrates ; Wedges</subject><ispartof>PloS one, 2018-04, Vol.13 (4), p.e0195385-e0195385</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Komura et al. 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In the face of this difficulty, DNA metabarcoding may be a useful approach to analyze diet compositions and foraging behaviors. Using this approach, we investigated the diet composition and its seasonal variation of a common seabird species on the Ogasawara Islands, Japan: the wedge-tailed shearwater Ardenna pacifica. We collected fecal samples during the prebreeding (N = 73) and rearing (N = 96) periods. The diet composition of wedge-tailed shearwater was analyzed by Ion Torrent sequencing using two universal polymerase chain reaction primers for the 12S and 16S mitochondrial DNA regions that targeted vertebrates and mollusks, respectively. The results of a BLAST search of obtained sequences detected 31 and 1 vertebrate and mollusk taxa, respectively. The results of the diet composition analysis showed that wedge-tailed shearwaters frequently consumed deep-sea fishes throughout the sampling season, indicating the importance of these fishes as a stable food resource. 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Future research that combines DNA metabarcoding with other tools, such as data logging, may provide further insight into the foraging ecology of pelagic seabirds.</description><subject>Animal behavior</subject><subject>Animals</subject><subject>Aquatic birds</subject><subject>Aquatic Organisms - genetics</subject><subject>Behavior</subject><subject>Biological research</subject><subject>Biology and Life Sciences</subject><subject>Birds - physiology</subject><subject>Breeding</subject><subject>Conservation</subject><subject>Data logging</subject><subject>Deep sea</subject><subject>Deoxyribonucleic acid</subject><subject>Depth indicators</subject><subject>Diet</subject><subject>DNA</subject><subject>DNA barcoding</subject><subject>DNA Barcoding, Taxonomic</subject><subject>Earth Sciences</subject><subject>Ecology</subject><subject>Ecology and Environmental Sciences</subject><subject>Ecosystem</subject><subject>Ecosystems</subject><subject>Exocoetidae</subject><subject>Feces</subject><subject>Feeding Behavior</subject><subject>Female</subject><subject>Fisheries</subject><subject>Fishes - 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In the face of this difficulty, DNA metabarcoding may be a useful approach to analyze diet compositions and foraging behaviors. Using this approach, we investigated the diet composition and its seasonal variation of a common seabird species on the Ogasawara Islands, Japan: the wedge-tailed shearwater Ardenna pacifica. We collected fecal samples during the prebreeding (N = 73) and rearing (N = 96) periods. The diet composition of wedge-tailed shearwater was analyzed by Ion Torrent sequencing using two universal polymerase chain reaction primers for the 12S and 16S mitochondrial DNA regions that targeted vertebrates and mollusks, respectively. The results of a BLAST search of obtained sequences detected 31 and 1 vertebrate and mollusk taxa, respectively. The results of the diet composition analysis showed that wedge-tailed shearwaters frequently consumed deep-sea fishes throughout the sampling season, indicating the importance of these fishes as a stable food resource. However, there was a marked seasonal shift in diet, which may reflect seasonal changes in food resource availability and wedge-tailed shearwater foraging behavior. The collected data regarding the shearwater diet may be useful for in situ conservation efforts. Future research that combines DNA metabarcoding with other tools, such as data logging, may provide further insight into the foraging ecology of pelagic seabirds.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29630670</pmid><doi>10.1371/journal.pone.0195385</doi><tpages>e0195385</tpages><orcidid>https://orcid.org/0000-0001-7491-5594</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Public Library of Science; Full-Text Journals in Chemistry (Open access); PubMed Central; Directory of Open Access Journals; EZB Electronic Journals Library |
| subjects | Animal behavior Animals Aquatic birds Aquatic Organisms - genetics Behavior Biological research Biology and Life Sciences Birds - physiology Breeding Conservation Data logging Deep sea Deoxyribonucleic acid Depth indicators Diet DNA DNA barcoding DNA Barcoding, Taxonomic Earth Sciences Ecology Ecology and Environmental Sciences Ecosystem Ecosystems Exocoetidae Feces Feeding Behavior Female Fisheries Fishes - genetics Fishing Food Food and nutrition Food availability Food Chain Foraging behavior Gene sequencing Hoarding (Animal feeding behavior) Islands Japan Male Medicine and Health Sciences Methods Mitochondrial DNA Mollusca - genetics Mollusks Oceans Polymerase chain reaction Population Predatory Behavior Primers Procellariidae Puffinus yelkouan Research and Analysis Methods Resource availability Seabirds Seasonal variations Seasons Shearwaters Studies Vertebrates Wedges |
| title | DNA barcoding reveals seasonal shifts in diet and consumption of deep-sea fishes in wedge-tailed shearwaters |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T16%3A26%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20barcoding%20reveals%20seasonal%20shifts%20in%20diet%20and%20consumption%20of%20deep-sea%20fishes%20in%20wedge-tailed%20shearwaters&rft.jtitle=PloS%20one&rft.au=Komura,%20Taketo&rft.date=2018-04-09&rft.volume=13&rft.issue=4&rft.spage=e0195385&rft.epage=e0195385&rft.pages=e0195385-e0195385&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0195385&rft_dat=%3Cgale_plos_%3EA533968990%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2023413929&rft_id=info:pmid/29630670&rft_galeid=A533968990&rft_doaj_id=oai_doaj_org_article_f430a089418f40fdb28bf0efba0e7843&rfr_iscdi=true |