Applying chlorogenic acid in an alginate scaffold of chondrocytes can improve the repair of damaged articular cartilage
Damaged cartilage has very low regenerative potential which has led to the search for novel tissue-engineering approaches to help treat cartilage defects. While various approaches have been reported, there is no perfect treatment currently. In this study we evaluated the effects of a plant extract,...
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| creator | Cheng, Xin Li, Ke Xu, Shengsong Li, Peizhi Yan, Yu Wang, Guang Berman, Zachary Guo, Rui Liang, Jianxin Traore, Sira Yang, Xuesong |
| description | Damaged cartilage has very low regenerative potential which has led to the search for novel tissue-engineering approaches to help treat cartilage defects. While various approaches have been reported, there is no perfect treatment currently. In this study we evaluated the effects of a plant extract, chlorogenic acid (CGA), as part of chondrocyte transplantation on a model of knee joint injury in chicks. First, primary cultured chondrocytes used to evaluate the effects of CGA on chondrogenesis. Then using an articular cartilage injury model of chick knee we assessed the functional recovery after transplantation of the complexes containing chondrocytes and CGA in an alginate scaffold. Histological analysis, PCR, and western blot were further used to understand the underlying mechanisms. We showed that 60 μM CGA in alginate exhibited notable effects on stimulating chondrogenesis in vitro. Secondly, it was shown that the application of these complexes accelerated the recovery of injury-induced dysfunction by gait analysis when followed for 21 days. Histochemical analysis demonstrated that there was less abnormal vasculature formation, more chondrocyte proliferation and cartilage matrix synthesis in the presence of the complexes containing CGA. We discovered CGA treated transplantation up-regulated the expressions of Sox9 and Col2a1 which were responsible for the stimulation of chondrogenesis. Furthermore, the application of these complexes could suppress the abnormal angiogenesis and fibrosis at the injury site. Lastly, the elevated levels of inflammatory cytokines IL-1β, TNF-α, p-p65, and MMPs expression were decreased in the presence of CGA. This may be caused through adjusting cellular redox homeostasis associated with Nrf2. This study suggests that combining chondrocytes and CGA on an alginate scaffold can improve the recovery of damaged articular cartilage. |
| doi_str_mv | 10.1371/journal.pone.0195326 |
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While various approaches have been reported, there is no perfect treatment currently. In this study we evaluated the effects of a plant extract, chlorogenic acid (CGA), as part of chondrocyte transplantation on a model of knee joint injury in chicks. First, primary cultured chondrocytes used to evaluate the effects of CGA on chondrogenesis. Then using an articular cartilage injury model of chick knee we assessed the functional recovery after transplantation of the complexes containing chondrocytes and CGA in an alginate scaffold. Histological analysis, PCR, and western blot were further used to understand the underlying mechanisms. We showed that 60 μM CGA in alginate exhibited notable effects on stimulating chondrogenesis in vitro. Secondly, it was shown that the application of these complexes accelerated the recovery of injury-induced dysfunction by gait analysis when followed for 21 days. Histochemical analysis demonstrated that there was less abnormal vasculature formation, more chondrocyte proliferation and cartilage matrix synthesis in the presence of the complexes containing CGA. We discovered CGA treated transplantation up-regulated the expressions of Sox9 and Col2a1 which were responsible for the stimulation of chondrogenesis. Furthermore, the application of these complexes could suppress the abnormal angiogenesis and fibrosis at the injury site. Lastly, the elevated levels of inflammatory cytokines IL-1β, TNF-α, p-p65, and MMPs expression were decreased in the presence of CGA. This may be caused through adjusting cellular redox homeostasis associated with Nrf2. This study suggests that combining chondrocytes and CGA on an alginate scaffold can improve the recovery of damaged articular cartilage.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0195326</identifier><identifier>PMID: 29621359</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Alginic acid ; Angiogenesis ; Arthritis ; Articular cartilage ; Biology and Life Sciences ; Care and treatment ; Cartilage ; Cartilage (articular) ; Cartilage cells ; Cell transplantation ; Chicks ; Chicks (Infant chickens) ; Chloroacetic acids ; Chlorogenic acid ; Chondrocytes ; Chondrogenesis ; Cytokines ; Damage ; Defects ; Embryology ; Fibrosis ; Gait ; Growth factors ; Health aspects ; Histochemical analysis ; Histology ; Homeostasis ; IL-1β ; Inflammation ; Injuries ; Injury analysis ; Juveniles ; Knee ; Laboratories ; Medicine ; Medicine and Health Sciences ; Oxidative stress ; Physiological aspects ; Plant extracts ; Recovery ; Recovery of function ; Research and Analysis Methods ; Rheumatism ; Rodents ; Scaffolds ; Sox9 protein ; Tissue engineering ; Transplantation ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α</subject><ispartof>PloS one, 2018-04, Vol.13 (4), p.e0195326-e0195326</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication: https://creativecommons.org/publicdomain/zero/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-4e0ed61a365430ccdaa5cfe15e12bf87c634f7dfda867c957f15d0b7a81cf7a3</citedby><cites>FETCH-LOGICAL-c692t-4e0ed61a365430ccdaa5cfe15e12bf87c634f7dfda867c957f15d0b7a81cf7a3</cites><orcidid>0000-0002-2537-5752</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5886530/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5886530/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29621359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lammi, Mikko Juhani</contributor><creatorcontrib>Cheng, Xin</creatorcontrib><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Xu, Shengsong</creatorcontrib><creatorcontrib>Li, Peizhi</creatorcontrib><creatorcontrib>Yan, Yu</creatorcontrib><creatorcontrib>Wang, Guang</creatorcontrib><creatorcontrib>Berman, Zachary</creatorcontrib><creatorcontrib>Guo, Rui</creatorcontrib><creatorcontrib>Liang, Jianxin</creatorcontrib><creatorcontrib>Traore, Sira</creatorcontrib><creatorcontrib>Yang, Xuesong</creatorcontrib><title>Applying chlorogenic acid in an alginate scaffold of chondrocytes can improve the repair of damaged articular cartilage</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Damaged cartilage has very low regenerative potential which has led to the search for novel tissue-engineering approaches to help treat cartilage defects. While various approaches have been reported, there is no perfect treatment currently. In this study we evaluated the effects of a plant extract, chlorogenic acid (CGA), as part of chondrocyte transplantation on a model of knee joint injury in chicks. First, primary cultured chondrocytes used to evaluate the effects of CGA on chondrogenesis. Then using an articular cartilage injury model of chick knee we assessed the functional recovery after transplantation of the complexes containing chondrocytes and CGA in an alginate scaffold. Histological analysis, PCR, and western blot were further used to understand the underlying mechanisms. We showed that 60 μM CGA in alginate exhibited notable effects on stimulating chondrogenesis in vitro. Secondly, it was shown that the application of these complexes accelerated the recovery of injury-induced dysfunction by gait analysis when followed for 21 days. Histochemical analysis demonstrated that there was less abnormal vasculature formation, more chondrocyte proliferation and cartilage matrix synthesis in the presence of the complexes containing CGA. We discovered CGA treated transplantation up-regulated the expressions of Sox9 and Col2a1 which were responsible for the stimulation of chondrogenesis. Furthermore, the application of these complexes could suppress the abnormal angiogenesis and fibrosis at the injury site. Lastly, the elevated levels of inflammatory cytokines IL-1β, TNF-α, p-p65, and MMPs expression were decreased in the presence of CGA. This may be caused through adjusting cellular redox homeostasis associated with Nrf2. This study suggests that combining chondrocytes and CGA on an alginate scaffold can improve the recovery of damaged articular cartilage.</description><subject>Acids</subject><subject>Alginic acid</subject><subject>Angiogenesis</subject><subject>Arthritis</subject><subject>Articular cartilage</subject><subject>Biology and Life Sciences</subject><subject>Care and treatment</subject><subject>Cartilage</subject><subject>Cartilage (articular)</subject><subject>Cartilage cells</subject><subject>Cell transplantation</subject><subject>Chicks</subject><subject>Chicks (Infant chickens)</subject><subject>Chloroacetic acids</subject><subject>Chlorogenic acid</subject><subject>Chondrocytes</subject><subject>Chondrogenesis</subject><subject>Cytokines</subject><subject>Damage</subject><subject>Defects</subject><subject>Embryology</subject><subject>Fibrosis</subject><subject>Gait</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Histochemical analysis</subject><subject>Histology</subject><subject>Homeostasis</subject><subject>IL-1β</subject><subject>Inflammation</subject><subject>Injuries</subject><subject>Injury analysis</subject><subject>Juveniles</subject><subject>Knee</subject><subject>Laboratories</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Oxidative stress</subject><subject>Physiological aspects</subject><subject>Plant extracts</subject><subject>Recovery</subject><subject>Recovery of function</subject><subject>Research and Analysis Methods</subject><subject>Rheumatism</subject><subject>Rodents</subject><subject>Scaffolds</subject><subject>Sox9 protein</subject><subject>Tissue engineering</subject><subject>Transplantation</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis 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chlorogenic acid in an alginate scaffold of chondrocytes can improve the repair of damaged articular cartilage</title><author>Cheng, Xin ; Li, Ke ; Xu, Shengsong ; Li, Peizhi ; Yan, Yu ; Wang, Guang ; Berman, Zachary ; Guo, Rui ; Liang, Jianxin ; Traore, Sira ; Yang, Xuesong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-4e0ed61a365430ccdaa5cfe15e12bf87c634f7dfda867c957f15d0b7a81cf7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acids</topic><topic>Alginic acid</topic><topic>Angiogenesis</topic><topic>Arthritis</topic><topic>Articular cartilage</topic><topic>Biology and Life Sciences</topic><topic>Care and treatment</topic><topic>Cartilage</topic><topic>Cartilage (articular)</topic><topic>Cartilage cells</topic><topic>Cell transplantation</topic><topic>Chicks</topic><topic>Chicks (Infant chickens)</topic><topic>Chloroacetic acids</topic><topic>Chlorogenic 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Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Xin</au><au>Li, Ke</au><au>Xu, Shengsong</au><au>Li, Peizhi</au><au>Yan, Yu</au><au>Wang, Guang</au><au>Berman, Zachary</au><au>Guo, Rui</au><au>Liang, Jianxin</au><au>Traore, Sira</au><au>Yang, Xuesong</au><au>Lammi, Mikko Juhani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Applying chlorogenic acid in an alginate scaffold of chondrocytes can improve the repair of damaged articular cartilage</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-04-05</date><risdate>2018</risdate><volume>13</volume><issue>4</issue><spage>e0195326</spage><epage>e0195326</epage><pages>e0195326-e0195326</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Damaged cartilage has very low regenerative potential which has led to the search for novel tissue-engineering approaches to help treat cartilage defects. While various approaches have been reported, there is no perfect treatment currently. In this study we evaluated the effects of a plant extract, chlorogenic acid (CGA), as part of chondrocyte transplantation on a model of knee joint injury in chicks. First, primary cultured chondrocytes used to evaluate the effects of CGA on chondrogenesis. Then using an articular cartilage injury model of chick knee we assessed the functional recovery after transplantation of the complexes containing chondrocytes and CGA in an alginate scaffold. Histological analysis, PCR, and western blot were further used to understand the underlying mechanisms. We showed that 60 μM CGA in alginate exhibited notable effects on stimulating chondrogenesis in vitro. Secondly, it was shown that the application of these complexes accelerated the recovery of injury-induced dysfunction by gait analysis when followed for 21 days. Histochemical analysis demonstrated that there was less abnormal vasculature formation, more chondrocyte proliferation and cartilage matrix synthesis in the presence of the complexes containing CGA. We discovered CGA treated transplantation up-regulated the expressions of Sox9 and Col2a1 which were responsible for the stimulation of chondrogenesis. Furthermore, the application of these complexes could suppress the abnormal angiogenesis and fibrosis at the injury site. Lastly, the elevated levels of inflammatory cytokines IL-1β, TNF-α, p-p65, and MMPs expression were decreased in the presence of CGA. This may be caused through adjusting cellular redox homeostasis associated with Nrf2. This study suggests that combining chondrocytes and CGA on an alginate scaffold can improve the recovery of damaged articular cartilage.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29621359</pmid><doi>10.1371/journal.pone.0195326</doi><tpages>e0195326</tpages><orcidid>https://orcid.org/0000-0002-2537-5752</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2018-04, Vol.13 (4), p.e0195326-e0195326 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2022123173 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Alginic acid Angiogenesis Arthritis Articular cartilage Biology and Life Sciences Care and treatment Cartilage Cartilage (articular) Cartilage cells Cell transplantation Chicks Chicks (Infant chickens) Chloroacetic acids Chlorogenic acid Chondrocytes Chondrogenesis Cytokines Damage Defects Embryology Fibrosis Gait Growth factors Health aspects Histochemical analysis Histology Homeostasis IL-1β Inflammation Injuries Injury analysis Juveniles Knee Laboratories Medicine Medicine and Health Sciences Oxidative stress Physiological aspects Plant extracts Recovery Recovery of function Research and Analysis Methods Rheumatism Rodents Scaffolds Sox9 protein Tissue engineering Transplantation Tumor necrosis factor-TNF Tumor necrosis factor-α |
| title | Applying chlorogenic acid in an alginate scaffold of chondrocytes can improve the repair of damaged articular cartilage |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T20%3A35%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Applying%20chlorogenic%20acid%20in%20an%20alginate%20scaffold%20of%20chondrocytes%20can%20improve%20the%20repair%20of%20damaged%20articular%20cartilage&rft.jtitle=PloS%20one&rft.au=Cheng,%20Xin&rft.date=2018-04-05&rft.volume=13&rft.issue=4&rft.spage=e0195326&rft.epage=e0195326&rft.pages=e0195326-e0195326&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0195326&rft_dat=%3Cgale_plos_%3EA533497543%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2022123173&rft_id=info:pmid/29621359&rft_galeid=A533497543&rft_doaj_id=oai_doaj_org_article_755f3b90a3b74cf8a0150a880b5c6de4&rfr_iscdi=true |