Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma
Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the...
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| creator | Choi, Jin Choi, Bo Kyong Kim, Jin Seok Lee, Jae-Won Park, Hyun Ah Ryu, Hyung Won Lee, Su Ui Hwang, Kwang Woo Yun, Won-Kee Kim, Hyoung-Chin Ahn, Kyung-Seop Oh, Sei-Ryang Lee, Hyun-Jun |
| description | Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), the levels of total immunoglobulin (Ig) E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13) and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias. |
| doi_str_mv | 10.1371/journal.pone.0167098 |
| format | Article |
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In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), the levels of total immunoglobulin (Ig) E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13) and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0167098</identifier><identifier>PMID: 27870920</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Airway management ; Alveoli ; Analysis ; Animal tissues ; Animals ; Asthma ; Asthma - chemically induced ; Asthma - immunology ; Asthma - pathology ; Asthma - prevention & control ; Biology and Life Sciences ; Biotechnology ; Bronchus ; Chronic obstructive pulmonary disease ; Cinnamates - chemistry ; Cinnamates - isolation & purification ; Cinnamates - pharmacology ; Cytokines ; Cytokines - immunology ; Dermatophagoides pteronyssinus ; Disease ; Disease Models, Animal ; Enzyme-linked immunosorbent assay ; Female ; GATA-3 protein ; GATA3 Transcription Factor - immunology ; Gene expression ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - immunology ; Helper cells ; House dust ; Immunoglobulin E ; Immunoglobulin G ; Inflammation ; Inflammatory diseases ; Interleukin 13 ; Interleukin 4 ; Interleukin 5 ; Iridoid Glucosides - chemistry ; Iridoid Glucosides - isolation & purification ; Iridoid Glucosides - pharmacology ; Laboratory animals ; Lung - immunology ; Lung - pathology ; Lungs ; Lymphocytes ; Lymphocytes T ; Medicine ; Medicine and Health Sciences ; Mice ; Mice, Inbred BALB C ; Mucus ; Pharmacy ; Pyroglyphidae ; Respiratory tract ; Respiratory tract diseases ; Th1 Cells - immunology ; Th1 Cells - pathology ; Th2 Cells - immunology ; Th2 Cells - pathology ; Tissue analysis ; Transcription factors ; γ-Interferon</subject><ispartof>PloS one, 2016-11, Vol.11 (11), p.e0167098-e0167098</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Choi et al 2016 Choi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-b6125be1b940c5fd83f1928e94dcbaca3377b7fe13ad343117d920dba423e91d3</citedby><cites>FETCH-LOGICAL-c725t-b6125be1b940c5fd83f1928e94dcbaca3377b7fe13ad343117d920dba423e91d3</cites><orcidid>0000-0003-1293-6159</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117775/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117775/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27870920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Jin</creatorcontrib><creatorcontrib>Choi, Bo Kyong</creatorcontrib><creatorcontrib>Kim, Jin Seok</creatorcontrib><creatorcontrib>Lee, Jae-Won</creatorcontrib><creatorcontrib>Park, Hyun Ah</creatorcontrib><creatorcontrib>Ryu, Hyung Won</creatorcontrib><creatorcontrib>Lee, Su Ui</creatorcontrib><creatorcontrib>Hwang, Kwang Woo</creatorcontrib><creatorcontrib>Yun, Won-Kee</creatorcontrib><creatorcontrib>Kim, Hyoung-Chin</creatorcontrib><creatorcontrib>Ahn, Kyung-Seop</creatorcontrib><creatorcontrib>Oh, Sei-Ryang</creatorcontrib><creatorcontrib>Lee, Hyun-Jun</creatorcontrib><title>Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), the levels of total immunoglobulin (Ig) E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13) and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias.</description><subject>Airway management</subject><subject>Alveoli</subject><subject>Analysis</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Asthma</subject><subject>Asthma - chemically induced</subject><subject>Asthma - immunology</subject><subject>Asthma - pathology</subject><subject>Asthma - prevention & control</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Bronchus</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Cinnamates - chemistry</subject><subject>Cinnamates - isolation & purification</subject><subject>Cinnamates - pharmacology</subject><subject>Cytokines</subject><subject>Cytokines - immunology</subject><subject>Dermatophagoides pteronyssinus</subject><subject>Disease</subject><subject>Disease Models, Animal</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>GATA-3 protein</subject><subject>GATA3 Transcription Factor - immunology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - immunology</subject><subject>Helper cells</subject><subject>House dust</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin G</subject><subject>Inflammation</subject><subject>Inflammatory diseases</subject><subject>Interleukin 13</subject><subject>Interleukin 4</subject><subject>Interleukin 5</subject><subject>Iridoid Glucosides - chemistry</subject><subject>Iridoid Glucosides - isolation & purification</subject><subject>Iridoid Glucosides - pharmacology</subject><subject>Laboratory animals</subject><subject>Lung - immunology</subject><subject>Lung - pathology</subject><subject>Lungs</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mucus</subject><subject>Pharmacy</subject><subject>Pyroglyphidae</subject><subject>Respiratory tract</subject><subject>Respiratory tract diseases</subject><subject>Th1 Cells - immunology</subject><subject>Th1 Cells - pathology</subject><subject>Th2 Cells - immunology</subject><subject>Th2 Cells - pathology</subject><subject>Tissue analysis</subject><subject>Transcription factors</subject><subject>γ-Interferon</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYmPwBggsISG4aPGfJE5ukKKObZE2DY3CreXYTuvh2MV2GH0hnhN366YV7WKKlEQnv_Pl-LO_LHuN4BQRij5dutFbbqYrZ9UUopLCunqS7aOa4EmJIXl6730vexHCJYQFqcryebaHaZVwDPezv1-18C5oqUDbgiZGZUceVQCN9ld8DVrbGz4MPGpnQbcGh-7KerUYTarYBYhLBeae2yC8Xl0zR1xE58FxM28I4FaC-RJPLlTilQSzdXQ_tU3y2gIOztwYVLpLZYDrwcnh2aS1chSJbIxRfqEFaEJcDvxl9qznJqhX2-dB9v3oy3x2Mjk9P25nzelEUFzESVciXHQKdXUORdHLivSoxpWqcyk6LjghlHa0V4hwSXKCEJXJBdnxHBNVI0kOsrc3uivjAttaHBiGGCGYY4oS0d4Q0vFLtvJ64H7NHNfsuuD8gnEftTCKiYqTjteyqvo6J1XfEcgLUuKuVFB0iiatz9u_jd2gpFA2em52RHe_WL1kC_ebFWlySosk8GEr4N2vUYXIBh2EMoZblbxlqCogrXKa149Ac1ymZZZlQt_9hz5sxJZa8LRWbXuXRhQbUdbkFFV1QfPNhNMHqHRJNWiRjm6vU32n4eNOQ2Ki-hMXfAyBtd8uHs-e_9hl399jl4qbuAzOjJtDG3bB_Abc5CJ41d_tB4Jsk7xbN9gmeWybvNT25v5e3jXdRo38A7mEKTg</recordid><startdate>20161121</startdate><enddate>20161121</enddate><creator>Choi, Jin</creator><creator>Choi, Bo Kyong</creator><creator>Kim, Jin Seok</creator><creator>Lee, Jae-Won</creator><creator>Park, Hyun Ah</creator><creator>Ryu, Hyung Won</creator><creator>Lee, Su Ui</creator><creator>Hwang, Kwang Woo</creator><creator>Yun, Won-Kee</creator><creator>Kim, Hyoung-Chin</creator><creator>Ahn, Kyung-Seop</creator><creator>Oh, Sei-Ryang</creator><creator>Lee, Hyun-Jun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1293-6159</orcidid></search><sort><creationdate>20161121</creationdate><title>Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma</title><author>Choi, Jin ; Choi, Bo Kyong ; Kim, Jin Seok ; Lee, Jae-Won ; Park, Hyun Ah ; Ryu, Hyung Won ; Lee, Su Ui ; Hwang, Kwang Woo ; Yun, Won-Kee ; Kim, Hyoung-Chin ; Ahn, Kyung-Seop ; Oh, Sei-Ryang ; Lee, Hyun-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-b6125be1b940c5fd83f1928e94dcbaca3377b7fe13ad343117d920dba423e91d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Airway management</topic><topic>Alveoli</topic><topic>Analysis</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Asthma</topic><topic>Asthma - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Jin</au><au>Choi, Bo Kyong</au><au>Kim, Jin Seok</au><au>Lee, Jae-Won</au><au>Park, Hyun Ah</au><au>Ryu, Hyung Won</au><au>Lee, Su Ui</au><au>Hwang, Kwang Woo</au><au>Yun, Won-Kee</au><au>Kim, Hyoung-Chin</au><au>Ahn, Kyung-Seop</au><au>Oh, Sei-Ryang</au><au>Lee, Hyun-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-11-21</date><risdate>2016</risdate><volume>11</volume><issue>11</issue><spage>e0167098</spage><epage>e0167098</epage><pages>e0167098-e0167098</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), the levels of total immunoglobulin (Ig) E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13) and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27870920</pmid><doi>10.1371/journal.pone.0167098</doi><tpages>e0167098</tpages><orcidid>https://orcid.org/0000-0003-1293-6159</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-11, Vol.11 (11), p.e0167098-e0167098 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2021104271 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Airway management Alveoli Analysis Animal tissues Animals Asthma Asthma - chemically induced Asthma - immunology Asthma - pathology Asthma - prevention & control Biology and Life Sciences Biotechnology Bronchus Chronic obstructive pulmonary disease Cinnamates - chemistry Cinnamates - isolation & purification Cinnamates - pharmacology Cytokines Cytokines - immunology Dermatophagoides pteronyssinus Disease Disease Models, Animal Enzyme-linked immunosorbent assay Female GATA-3 protein GATA3 Transcription Factor - immunology Gene expression Gene Expression Regulation - drug effects Gene Expression Regulation - immunology Helper cells House dust Immunoglobulin E Immunoglobulin G Inflammation Inflammatory diseases Interleukin 13 Interleukin 4 Interleukin 5 Iridoid Glucosides - chemistry Iridoid Glucosides - isolation & purification Iridoid Glucosides - pharmacology Laboratory animals Lung - immunology Lung - pathology Lungs Lymphocytes Lymphocytes T Medicine Medicine and Health Sciences Mice Mice, Inbred BALB C Mucus Pharmacy Pyroglyphidae Respiratory tract Respiratory tract diseases Th1 Cells - immunology Th1 Cells - pathology Th2 Cells - immunology Th2 Cells - pathology Tissue analysis Transcription factors γ-Interferon |
| title | Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma |
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