Complete chloroplast genome sequence and comparative analysis of loblolly pine (Pinus taeda L.) with related species
Pinaceae, the largest family of conifers, has a diversified organization of chloroplast (cp) genomes with two typical highly reduced inverted repeats (IRs). In the current study, we determined the complete sequence of the cp genome of an economically and ecologically important conifer tree, the lobl...
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| description | Pinaceae, the largest family of conifers, has a diversified organization of chloroplast (cp) genomes with two typical highly reduced inverted repeats (IRs). In the current study, we determined the complete sequence of the cp genome of an economically and ecologically important conifer tree, the loblolly pine (Pinus taeda L.), using Illumina paired-end sequencing and compared the sequence with those of other pine species. The results revealed a genome size of 121,531 base pairs (bp) containing a pair of 830-bp IR regions, distinguished by a small single copy (42,258 bp) and large single copy (77,614 bp) region. The chloroplast genome of P. taeda encodes 120 genes, comprising 81 protein-coding genes, four ribosomal RNA genes, and 35 tRNA genes, with 151 randomly distributed microsatellites. Approximately 6 palindromic, 34 forward, and 22 tandem repeats were found in the P. taeda cp genome. Whole cp genome comparison with those of other Pinus species exhibited an overall high degree of sequence similarity, with some divergence in intergenic spacers. Higher and lower numbers of indels and single-nucleotide polymorphism substitutions were observed relative to P. contorta and P. monophylla, respectively. Phylogenomic analyses based on the complete genome sequence revealed that 60 shared genes generated trees with the same topologies, and P. taeda was closely related to P. contorta in the subgenus Pinus. Thus, the complete P. taeda genome provided valuable resources for population and evolutionary studies of gymnosperms and can be used to identify related species. |
| doi_str_mv | 10.1371/journal.pone.0192966 |
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In the current study, we determined the complete sequence of the cp genome of an economically and ecologically important conifer tree, the loblolly pine (Pinus taeda L.), using Illumina paired-end sequencing and compared the sequence with those of other pine species. The results revealed a genome size of 121,531 base pairs (bp) containing a pair of 830-bp IR regions, distinguished by a small single copy (42,258 bp) and large single copy (77,614 bp) region. The chloroplast genome of P. taeda encodes 120 genes, comprising 81 protein-coding genes, four ribosomal RNA genes, and 35 tRNA genes, with 151 randomly distributed microsatellites. Approximately 6 palindromic, 34 forward, and 22 tandem repeats were found in the P. taeda cp genome. Whole cp genome comparison with those of other Pinus species exhibited an overall high degree of sequence similarity, with some divergence in intergenic spacers. Higher and lower numbers of indels and single-nucleotide polymorphism substitutions were observed relative to P. contorta and P. monophylla, respectively. Phylogenomic analyses based on the complete genome sequence revealed that 60 shared genes generated trees with the same topologies, and P. taeda was closely related to P. contorta in the subgenus Pinus. Thus, the complete P. taeda genome provided valuable resources for population and evolutionary studies of gymnosperms and can be used to identify related species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0192966</identifier><identifier>PMID: 29596414</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Base pairs ; Biogeography ; Biological evolution ; Biology and Life Sciences ; Chloroplasts ; Comparative analysis ; Computer and Information Sciences ; Coniferophyta ; Coniferous trees ; Conifers ; Deoxyribonucleic acid ; Divergence ; DNA ; Ethanol ; Evolution ; Genes ; Genetic aspects ; Genetic testing ; Genetics ; Genomes ; Gymnosperms ; Herbal medicine ; Microsatellites ; Natural products ; Nucleotide sequence ; Phylogenetics ; Pinaceae ; Pine ; Pine trees ; Pines ; Pinus ; Pinus contorta ; Pinus monophylla ; Pinus taeda ; Plant sciences ; Plant taxonomy ; Polymorphism ; Population studies ; Properties ; Ribonucleic acid ; RNA ; rRNA ; Single-nucleotide polymorphism ; Soybeans ; Species ; tRNA</subject><ispartof>PloS one, 2018-03, Vol.13 (3), p.e0192966-e0192966</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Asaf et al. 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Higher and lower numbers of indels and single-nucleotide polymorphism substitutions were observed relative to P. contorta and P. monophylla, respectively. Phylogenomic analyses based on the complete genome sequence revealed that 60 shared genes generated trees with the same topologies, and P. taeda was closely related to P. contorta in the subgenus Pinus. 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asaf, Sajjad</au><au>Khan, Abdul Latif</au><au>Khan, Muhammad Aaqil</au><au>Shahzad, Raheem</au><au>Lubna</au><au>Kang, Sang Mo</au><au>Al-Harrasi, Ahmed</au><au>Al-Rawahi, Ahmed</au><au>Lee, In-Jung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete chloroplast genome sequence and comparative analysis of loblolly pine (Pinus taeda L.) with related species</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-03-29</date><risdate>2018</risdate><volume>13</volume><issue>3</issue><spage>e0192966</spage><epage>e0192966</epage><pages>e0192966-e0192966</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pinaceae, the largest family of conifers, has a diversified organization of chloroplast (cp) genomes with two typical highly reduced inverted repeats (IRs). In the current study, we determined the complete sequence of the cp genome of an economically and ecologically important conifer tree, the loblolly pine (Pinus taeda L.), using Illumina paired-end sequencing and compared the sequence with those of other pine species. The results revealed a genome size of 121,531 base pairs (bp) containing a pair of 830-bp IR regions, distinguished by a small single copy (42,258 bp) and large single copy (77,614 bp) region. The chloroplast genome of P. taeda encodes 120 genes, comprising 81 protein-coding genes, four ribosomal RNA genes, and 35 tRNA genes, with 151 randomly distributed microsatellites. Approximately 6 palindromic, 34 forward, and 22 tandem repeats were found in the P. taeda cp genome. Whole cp genome comparison with those of other Pinus species exhibited an overall high degree of sequence similarity, with some divergence in intergenic spacers. Higher and lower numbers of indels and single-nucleotide polymorphism substitutions were observed relative to P. contorta and P. monophylla, respectively. Phylogenomic analyses based on the complete genome sequence revealed that 60 shared genes generated trees with the same topologies, and P. taeda was closely related to P. contorta in the subgenus Pinus. Thus, the complete P. taeda genome provided valuable resources for population and evolutionary studies of gymnosperms and can be used to identify related species.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29596414</pmid><doi>10.1371/journal.pone.0192966</doi><orcidid>https://orcid.org/0000-0001-8216-7549</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Base pairs Biogeography Biological evolution Biology and Life Sciences Chloroplasts Comparative analysis Computer and Information Sciences Coniferophyta Coniferous trees Conifers Deoxyribonucleic acid Divergence DNA Ethanol Evolution Genes Genetic aspects Genetic testing Genetics Genomes Gymnosperms Herbal medicine Microsatellites Natural products Nucleotide sequence Phylogenetics Pinaceae Pine Pine trees Pines Pinus Pinus contorta Pinus monophylla Pinus taeda Plant sciences Plant taxonomy Polymorphism Population studies Properties Ribonucleic acid RNA rRNA Single-nucleotide polymorphism Soybeans Species tRNA |
| title | Complete chloroplast genome sequence and comparative analysis of loblolly pine (Pinus taeda L.) with related species |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T08%3A57%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Complete%20chloroplast%20genome%20sequence%20and%20comparative%20analysis%20of%20loblolly%20pine%20(Pinus%20taeda%20L.)%20with%20related%20species&rft.jtitle=PloS%20one&rft.au=Asaf,%20Sajjad&rft.date=2018-03-29&rft.volume=13&rft.issue=3&rft.spage=e0192966&rft.epage=e0192966&rft.pages=e0192966-e0192966&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0192966&rft_dat=%3Cgale_plos_%3EA532722892%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2019803128&rft_id=info:pmid/29596414&rft_galeid=A532722892&rft_doaj_id=oai_doaj_org_article_53bd00df7dcd4da8bd29491aedbddde8&rfr_iscdi=true |