Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis
Analyzing extracellular vesicles (EVs) is an attractive approach to diagnosis of prostate diagnosis. However, existing methods of EVs isolation have low efficiency, purity, and long process time, and therefore have low diagnostic ability. To solve these the problems, a two-phase system is adapted to...
Gespeichert in:
| Veröffentlicht in: | PloS one 2018-03, Vol.13 (3), p.e0194818-e0194818 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0194818 |
|---|---|
| container_issue | 3 |
| container_start_page | e0194818 |
| container_title | PloS one |
| container_volume | 13 |
| creator | Shin, Hyunwoo Park, Yong Hyun Kim, Yong-Goo Lee, Ji Youl Park, Jaesung |
| description | Analyzing extracellular vesicles (EVs) is an attractive approach to diagnosis of prostate diagnosis. However, existing methods of EVs isolation have low efficiency, purity, and long process time, and therefore have low diagnostic ability. To solve these the problems, a two-phase system is adapted to isolate EVs from a patient's urine. Urine from 20 prostate cancer (PCA) patients and 10 benign prostate hyperplasia patients was used to quantify the EVs-isolation ability of an aqueous two-phase system (ATPS) and to compare the diagnostic ability of ATPS with that of the conventional diagnosis method. An optimized ATPS isolates EVs with ~100% efficiency within ~30 min, with 14 times as high as achieved by ultracentrifugation. Afterward, PCR and ELISA are used to detect EVs derived from PCA cells in urine. The results demonstrate that diagnostic ability based on ATPS is better than other conventional diagnostic methods. ATPS can obtain a high quality and quantity of EVs from patients' urine. EVs contain cancer-related protein and genes, so these abundant sources enable diagnosis with high specificity and sensitivity. Therefore, ATPS is a useful tool to increase the specificity and sensitivity of diagnosis. |
| doi_str_mv | 10.1371/journal.pone.0194818 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2019025842</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A532485901</galeid><doaj_id>oai_doaj_org_article_3604d9ee7d5c44ae878c0e910c79eda1</doaj_id><sourcerecordid>A532485901</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-98b06eeb60a58c58d0a8c34492d0c8e121c8a998d8ec902b939aeabdf5dd02953</originalsourceid><addsrcrecordid>eNqNk9tq3DAQhk1padK0b1BaQ6G0F95KPkm6KSyhh4VAoKdbMSuNd7XY1kaS0-btK2edsC65KL6wkb_5NfPPTJK8pGRBC0Y_7OzgemgXe9vjglBRcsofJadUFHlW56R4fPR9kjzzfkdIVfC6fpqc5KLiJWPsNFHLqwHt4NPw22b7LXhM_Y0P2KXBpsbbFgKm-Cc4UNi2QwsuvUZvVIs-bZzt0sGZHtPGunTvrA8jrqBX6FJtYNNbb_zz5EkDrccX0_ss-fn504_zr9nF5ZfV-fIiU6ziIRN8TWrEdU2g4qrimgBXRVmKXBPFkeZUcRCCa45KkHwtCgEIa91UWpNYUXGWvD7o7lvr5eSPl3k0h-Sx4DwSqwOhLezk3pkO3I20YOTtgXUbCS6M1cmiJqUWiExXqiwBOeOKoKBEMYEaaNT6ON02rDvUCvtoUjsTnf_pzVZu7LWsOCOCjcm8mwScjU3wQXbGjy5DP3bkNu-yZrxmEX3zD_pwdRO1gViA6Rs7tm0UlcuqyEteCTLmvXiAio_Gzqg4TI2J57OA97OAyIQ4ERsYvJer79_-n738NWffHrFbhDZs48ANwdjez8HyAKo4Yd5hc28yJXLchTs35LgLctqFGPbquEH3QXfDX_wF-cwF4g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2019025842</pqid></control><display><type>article</type><title>Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis</title><source>PLoS</source><source>PubMed Central</source><source>Directory of Open Access Journals</source><source>Free Full-Text Journals in Chemistry</source><source>EZB Electronic Journals Library</source><creator>Shin, Hyunwoo ; Park, Yong Hyun ; Kim, Yong-Goo ; Lee, Ji Youl ; Park, Jaesung</creator><contributor>Ahmad, Aamir</contributor><creatorcontrib>Shin, Hyunwoo ; Park, Yong Hyun ; Kim, Yong-Goo ; Lee, Ji Youl ; Park, Jaesung ; Ahmad, Aamir</creatorcontrib><description>Analyzing extracellular vesicles (EVs) is an attractive approach to diagnosis of prostate diagnosis. However, existing methods of EVs isolation have low efficiency, purity, and long process time, and therefore have low diagnostic ability. To solve these the problems, a two-phase system is adapted to isolate EVs from a patient's urine. Urine from 20 prostate cancer (PCA) patients and 10 benign prostate hyperplasia patients was used to quantify the EVs-isolation ability of an aqueous two-phase system (ATPS) and to compare the diagnostic ability of ATPS with that of the conventional diagnosis method. An optimized ATPS isolates EVs with ~100% efficiency within ~30 min, with 14 times as high as achieved by ultracentrifugation. Afterward, PCR and ELISA are used to detect EVs derived from PCA cells in urine. The results demonstrate that diagnostic ability based on ATPS is better than other conventional diagnostic methods. ATPS can obtain a high quality and quantity of EVs from patients' urine. EVs contain cancer-related protein and genes, so these abundant sources enable diagnosis with high specificity and sensitivity. Therefore, ATPS is a useful tool to increase the specificity and sensitivity of diagnosis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0194818</identifier><identifier>PMID: 29584777</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Binary systems ; Binary systems (materials) ; Biochemistry ; Biology and Life Sciences ; Cancer ; Chromatography ; Diagnosis ; Diagnostic systems ; Earth Sciences ; Enzyme-linked immunosorbent assay ; Extracellular vesicles ; Hyperplasia ; Identification and classification ; Innovations ; Mechanical engineering ; Medical diagnosis ; Medicine and Health Sciences ; Methods ; Microorganisms ; Molecular diagnostic techniques ; Organelles ; Patients ; Physical Sciences ; Polyethylene glycol ; Polymers ; Prostate cancer ; Proteins ; Research and analysis methods ; Sediments ; Sensitivity ; Ultracentrifugation ; Urine ; Urology ; Vesicles</subject><ispartof>PloS one, 2018-03, Vol.13 (3), p.e0194818-e0194818</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Shin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Shin et al 2018 Shin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-98b06eeb60a58c58d0a8c34492d0c8e121c8a998d8ec902b939aeabdf5dd02953</citedby><cites>FETCH-LOGICAL-c758t-98b06eeb60a58c58d0a8c34492d0c8e121c8a998d8ec902b939aeabdf5dd02953</cites><orcidid>0000-0002-5082-3318</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870972/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870972/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23868,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29584777$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ahmad, Aamir</contributor><creatorcontrib>Shin, Hyunwoo</creatorcontrib><creatorcontrib>Park, Yong Hyun</creatorcontrib><creatorcontrib>Kim, Yong-Goo</creatorcontrib><creatorcontrib>Lee, Ji Youl</creatorcontrib><creatorcontrib>Park, Jaesung</creatorcontrib><title>Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Analyzing extracellular vesicles (EVs) is an attractive approach to diagnosis of prostate diagnosis. However, existing methods of EVs isolation have low efficiency, purity, and long process time, and therefore have low diagnostic ability. To solve these the problems, a two-phase system is adapted to isolate EVs from a patient's urine. Urine from 20 prostate cancer (PCA) patients and 10 benign prostate hyperplasia patients was used to quantify the EVs-isolation ability of an aqueous two-phase system (ATPS) and to compare the diagnostic ability of ATPS with that of the conventional diagnosis method. An optimized ATPS isolates EVs with ~100% efficiency within ~30 min, with 14 times as high as achieved by ultracentrifugation. Afterward, PCR and ELISA are used to detect EVs derived from PCA cells in urine. The results demonstrate that diagnostic ability based on ATPS is better than other conventional diagnostic methods. ATPS can obtain a high quality and quantity of EVs from patients' urine. EVs contain cancer-related protein and genes, so these abundant sources enable diagnosis with high specificity and sensitivity. Therefore, ATPS is a useful tool to increase the specificity and sensitivity of diagnosis.</description><subject>Binary systems</subject><subject>Binary systems (materials)</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Cancer</subject><subject>Chromatography</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Earth Sciences</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Extracellular vesicles</subject><subject>Hyperplasia</subject><subject>Identification and classification</subject><subject>Innovations</subject><subject>Mechanical engineering</subject><subject>Medical diagnosis</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Microorganisms</subject><subject>Molecular diagnostic techniques</subject><subject>Organelles</subject><subject>Patients</subject><subject>Physical Sciences</subject><subject>Polyethylene glycol</subject><subject>Polymers</subject><subject>Prostate cancer</subject><subject>Proteins</subject><subject>Research and analysis methods</subject><subject>Sediments</subject><subject>Sensitivity</subject><subject>Ultracentrifugation</subject><subject>Urine</subject><subject>Urology</subject><subject>Vesicles</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQ6G0F95KPkm6KSyhh4VAoKdbMSuNd7XY1kaS0-btK2edsC65KL6wkb_5NfPPTJK8pGRBC0Y_7OzgemgXe9vjglBRcsofJadUFHlW56R4fPR9kjzzfkdIVfC6fpqc5KLiJWPsNFHLqwHt4NPw22b7LXhM_Y0P2KXBpsbbFgKm-Cc4UNi2QwsuvUZvVIs-bZzt0sGZHtPGunTvrA8jrqBX6FJtYNNbb_zz5EkDrccX0_ss-fn504_zr9nF5ZfV-fIiU6ziIRN8TWrEdU2g4qrimgBXRVmKXBPFkeZUcRCCa45KkHwtCgEIa91UWpNYUXGWvD7o7lvr5eSPl3k0h-Sx4DwSqwOhLezk3pkO3I20YOTtgXUbCS6M1cmiJqUWiExXqiwBOeOKoKBEMYEaaNT6ON02rDvUCvtoUjsTnf_pzVZu7LWsOCOCjcm8mwScjU3wQXbGjy5DP3bkNu-yZrxmEX3zD_pwdRO1gViA6Rs7tm0UlcuqyEteCTLmvXiAio_Gzqg4TI2J57OA97OAyIQ4ERsYvJer79_-n738NWffHrFbhDZs48ANwdjez8HyAKo4Yd5hc28yJXLchTs35LgLctqFGPbquEH3QXfDX_wF-cwF4g</recordid><startdate>20180327</startdate><enddate>20180327</enddate><creator>Shin, Hyunwoo</creator><creator>Park, Yong Hyun</creator><creator>Kim, Yong-Goo</creator><creator>Lee, Ji Youl</creator><creator>Park, Jaesung</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5082-3318</orcidid></search><sort><creationdate>20180327</creationdate><title>Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis</title><author>Shin, Hyunwoo ; Park, Yong Hyun ; Kim, Yong-Goo ; Lee, Ji Youl ; Park, Jaesung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-98b06eeb60a58c58d0a8c34492d0c8e121c8a998d8ec902b939aeabdf5dd02953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Binary systems</topic><topic>Binary systems (materials)</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Cancer</topic><topic>Chromatography</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Earth Sciences</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Extracellular vesicles</topic><topic>Hyperplasia</topic><topic>Identification and classification</topic><topic>Innovations</topic><topic>Mechanical engineering</topic><topic>Medical diagnosis</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Microorganisms</topic><topic>Molecular diagnostic techniques</topic><topic>Organelles</topic><topic>Patients</topic><topic>Physical Sciences</topic><topic>Polyethylene glycol</topic><topic>Polymers</topic><topic>Prostate cancer</topic><topic>Proteins</topic><topic>Research and analysis methods</topic><topic>Sediments</topic><topic>Sensitivity</topic><topic>Ultracentrifugation</topic><topic>Urine</topic><topic>Urology</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shin, Hyunwoo</creatorcontrib><creatorcontrib>Park, Yong Hyun</creatorcontrib><creatorcontrib>Kim, Yong-Goo</creatorcontrib><creatorcontrib>Lee, Ji Youl</creatorcontrib><creatorcontrib>Park, Jaesung</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database (ProQuest)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shin, Hyunwoo</au><au>Park, Yong Hyun</au><au>Kim, Yong-Goo</au><au>Lee, Ji Youl</au><au>Park, Jaesung</au><au>Ahmad, Aamir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-03-27</date><risdate>2018</risdate><volume>13</volume><issue>3</issue><spage>e0194818</spage><epage>e0194818</epage><pages>e0194818-e0194818</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Analyzing extracellular vesicles (EVs) is an attractive approach to diagnosis of prostate diagnosis. However, existing methods of EVs isolation have low efficiency, purity, and long process time, and therefore have low diagnostic ability. To solve these the problems, a two-phase system is adapted to isolate EVs from a patient's urine. Urine from 20 prostate cancer (PCA) patients and 10 benign prostate hyperplasia patients was used to quantify the EVs-isolation ability of an aqueous two-phase system (ATPS) and to compare the diagnostic ability of ATPS with that of the conventional diagnosis method. An optimized ATPS isolates EVs with ~100% efficiency within ~30 min, with 14 times as high as achieved by ultracentrifugation. Afterward, PCR and ELISA are used to detect EVs derived from PCA cells in urine. The results demonstrate that diagnostic ability based on ATPS is better than other conventional diagnostic methods. ATPS can obtain a high quality and quantity of EVs from patients' urine. EVs contain cancer-related protein and genes, so these abundant sources enable diagnosis with high specificity and sensitivity. Therefore, ATPS is a useful tool to increase the specificity and sensitivity of diagnosis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29584777</pmid><doi>10.1371/journal.pone.0194818</doi><tpages>e0194818</tpages><orcidid>https://orcid.org/0000-0002-5082-3318</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-03, Vol.13 (3), p.e0194818-e0194818 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2019025842 |
| source | PLoS; PubMed Central; Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Binary systems Binary systems (materials) Biochemistry Biology and Life Sciences Cancer Chromatography Diagnosis Diagnostic systems Earth Sciences Enzyme-linked immunosorbent assay Extracellular vesicles Hyperplasia Identification and classification Innovations Mechanical engineering Medical diagnosis Medicine and Health Sciences Methods Microorganisms Molecular diagnostic techniques Organelles Patients Physical Sciences Polyethylene glycol Polymers Prostate cancer Proteins Research and analysis methods Sediments Sensitivity Ultracentrifugation Urine Urology Vesicles |
| title | Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T20%3A39%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Aqueous%20two-phase%20system%20to%20isolate%20extracellular%20vesicles%20from%20urine%20for%20prostate%20cancer%20diagnosis&rft.jtitle=PloS%20one&rft.au=Shin,%20Hyunwoo&rft.date=2018-03-27&rft.volume=13&rft.issue=3&rft.spage=e0194818&rft.epage=e0194818&rft.pages=e0194818-e0194818&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0194818&rft_dat=%3Cgale_plos_%3EA532485901%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2019025842&rft_id=info:pmid/29584777&rft_galeid=A532485901&rft_doaj_id=oai_doaj_org_article_3604d9ee7d5c44ae878c0e910c79eda1&rfr_iscdi=true |