Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells

Chinese hamster ovary (CHO) cells are the most frequently used host for commercial production of therapeutic proteins. However, their low protein productivity in culture is the main hurdle to overcome. Mild hypothermia has been established as an effective strategy to enhance protein specific product...

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Veröffentlicht in:	PloS one 2018-03, Vol.13 (3), p.e0194510-e0194510
Hauptverfasser:	Torres, Mauro, Zúñiga, Roberto, Gutierrez, Matias, Vergara, Mauricio, Collazo, Norberto, Reyes, Juan, Berrios, Julio, Aguillon, Juan Carlos, Molina, Maria Carmen, Altamirano, Claudia
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - metabolism Batch processes Biology and Life Sciences Biotechnology - methods Carbon Cell culture Cell Culture Techniques - methods Cell cycle Cell density Cell growth Cell lines Cell Proliferation - physiology Cell Survival - physiology CHO Cells Cold Temperature Cricetinae Cricetulus Endoplasmic reticulum Gene expression Gene regulation Genetic engineering Glutamine Growth rate Humans Hypothermia Lactic acid Low temperature Medicine and Health Sciences Metabolism Metabolites Myc protein Physical Sciences Productivity Proteins Proto-Oncogene Proteins c-myc - metabolism Recombinant Proteins - metabolism Research and analysis methods Temperature Temperature effects Toxicity Transcription factors Tumor Necrosis Factor-alpha - antagonists & inhibitors Tumor Necrosis Factor-alpha - immunology Tumor necrosis factor-α Up-Regulation X-Box Binding Protein 1 - metabolism
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