Persistent effectivity of gas plasma-treated, long time-stored liquid on epithelial cell adhesion capacity and membrane morphology
Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liq...
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| creator | Hoentsch, Maxi Bussiahn, René Rebl, Henrike Bergemann, Claudia Eggert, Martin Frank, Marcus von Woedtke, Thomas Nebe, Barbara |
| description | Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up to seven days; i. e. the liquid preserves the characteristics once induced by the argon plasma. Therefore, the complete Dulbecco's Modified Eagle cell culture medium was argon plasma-treated (atmospheric pressure, kINPen09) for 60 s, stored for several days (1, 4 and 7 d) at 37°C and added to a confluent mouse hepatocyte epithelial cell (mHepR1) monolayer. Impaired tight junction architecture as well as shortened microvilli on the cell membrane could be observed, which was accompanied by the loss of cell adhesion capacity. Online-monitoring of vital cells revealed a reduced cell respiration. Our first time-dependent analysis of plasma-treated medium revealed that temperature, hydrogen peroxide production, pH and oxygen content can be excluded as initiators of cell physiological and morphological changes. The here observed persisting biological effects in plasma-treated liquids could open new medical applications in dentistry and orthopaedics. |
| doi_str_mv | 10.1371/journal.pone.0104559 |
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The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up to seven days; i. e. the liquid preserves the characteristics once induced by the argon plasma. Therefore, the complete Dulbecco's Modified Eagle cell culture medium was argon plasma-treated (atmospheric pressure, kINPen09) for 60 s, stored for several days (1, 4 and 7 d) at 37°C and added to a confluent mouse hepatocyte epithelial cell (mHepR1) monolayer. Impaired tight junction architecture as well as shortened microvilli on the cell membrane could be observed, which was accompanied by the loss of cell adhesion capacity. Online-monitoring of vital cells revealed a reduced cell respiration. Our first time-dependent analysis of plasma-treated medium revealed that temperature, hydrogen peroxide production, pH and oxygen content can be excluded as initiators of cell physiological and morphological changes. The here observed persisting biological effects in plasma-treated liquids could open new medical applications in dentistry and orthopaedics.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0104559</identifier><identifier>PMID: 25170906</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adhesion ; Animals ; Antimicrobial agents ; Argon ; Argon - metabolism ; Argon plasma ; Atmospheric pressure ; Bacteria ; Biological effects ; Biology ; Biology and Life Sciences ; Cell Adhesion ; Cell adhesion & migration ; Cell culture ; Cell interactions ; Cell Line ; Cell Membrane - metabolism ; Cell Membrane - ultrastructure ; Cell Survival ; Culture Media - metabolism ; Cytology ; Dentistry ; E coli ; Epithelial cells ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Epithelial Cells - ultrastructure ; Escherichia coli ; Gases ; Gases - metabolism ; Gram-positive bacteria ; Hepatocytes - cytology ; Hepatocytes - metabolism ; Hepatocytes - ultrastructure ; Hydrogen ; Hydrogen peroxide ; Hydrogen storage ; Initiators ; Medicine ; Medicine and Health Sciences ; Mice ; Microvilli - metabolism ; Microvilli - ultrastructure ; Morphology ; Oxygen ; Oxygen content ; Physical Sciences ; Physiological effects ; Physiology ; Plasma ; Preservation, Biological - methods ; Research and Analysis Methods ; Tight Junctions - metabolism ; Tight Junctions - ultrastructure ; Time dependent analysis</subject><ispartof>PloS one, 2014-08, Vol.9 (8), p.e104559-e104559</ispartof><rights>2014 Hoentsch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Hoentsch et al 2014 Hoentsch et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-227c6d4df3e9052117255ffebd1113b503c774d0df7d3056a9baa9118eb6bb473</citedby><cites>FETCH-LOGICAL-c592t-227c6d4df3e9052117255ffebd1113b503c774d0df7d3056a9baa9118eb6bb473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149358/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149358/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25170906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yousfi, Mohammed</contributor><creatorcontrib>Hoentsch, Maxi</creatorcontrib><creatorcontrib>Bussiahn, René</creatorcontrib><creatorcontrib>Rebl, Henrike</creatorcontrib><creatorcontrib>Bergemann, Claudia</creatorcontrib><creatorcontrib>Eggert, Martin</creatorcontrib><creatorcontrib>Frank, Marcus</creatorcontrib><creatorcontrib>von Woedtke, Thomas</creatorcontrib><creatorcontrib>Nebe, Barbara</creatorcontrib><title>Persistent effectivity of gas plasma-treated, long time-stored liquid on epithelial cell adhesion capacity and membrane morphology</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up to seven days; i. e. the liquid preserves the characteristics once induced by the argon plasma. Therefore, the complete Dulbecco's Modified Eagle cell culture medium was argon plasma-treated (atmospheric pressure, kINPen09) for 60 s, stored for several days (1, 4 and 7 d) at 37°C and added to a confluent mouse hepatocyte epithelial cell (mHepR1) monolayer. Impaired tight junction architecture as well as shortened microvilli on the cell membrane could be observed, which was accompanied by the loss of cell adhesion capacity. Online-monitoring of vital cells revealed a reduced cell respiration. 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The here observed persisting biological effects in plasma-treated liquids could open new medical applications in dentistry and orthopaedics.</description><subject>Adhesion</subject><subject>Animals</subject><subject>Antimicrobial agents</subject><subject>Argon</subject><subject>Argon - metabolism</subject><subject>Argon plasma</subject><subject>Atmospheric pressure</subject><subject>Bacteria</subject><subject>Biological effects</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell interactions</subject><subject>Cell Line</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Survival</subject><subject>Culture Media - metabolism</subject><subject>Cytology</subject><subject>Dentistry</subject><subject>E coli</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - 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The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up to seven days; i. e. the liquid preserves the characteristics once induced by the argon plasma. Therefore, the complete Dulbecco's Modified Eagle cell culture medium was argon plasma-treated (atmospheric pressure, kINPen09) for 60 s, stored for several days (1, 4 and 7 d) at 37°C and added to a confluent mouse hepatocyte epithelial cell (mHepR1) monolayer. Impaired tight junction architecture as well as shortened microvilli on the cell membrane could be observed, which was accompanied by the loss of cell adhesion capacity. Online-monitoring of vital cells revealed a reduced cell respiration. Our first time-dependent analysis of plasma-treated medium revealed that temperature, hydrogen peroxide production, pH and oxygen content can be excluded as initiators of cell physiological and morphological changes. The here observed persisting biological effects in plasma-treated liquids could open new medical applications in dentistry and orthopaedics.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25170906</pmid><doi>10.1371/journal.pone.0104559</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesion Animals Antimicrobial agents Argon Argon - metabolism Argon plasma Atmospheric pressure Bacteria Biological effects Biology Biology and Life Sciences Cell Adhesion Cell adhesion & migration Cell culture Cell interactions Cell Line Cell Membrane - metabolism Cell Membrane - ultrastructure Cell Survival Culture Media - metabolism Cytology Dentistry E coli Epithelial cells Epithelial Cells - cytology Epithelial Cells - metabolism Epithelial Cells - ultrastructure Escherichia coli Gases Gases - metabolism Gram-positive bacteria Hepatocytes - cytology Hepatocytes - metabolism Hepatocytes - ultrastructure Hydrogen Hydrogen peroxide Hydrogen storage Initiators Medicine Medicine and Health Sciences Mice Microvilli - metabolism Microvilli - ultrastructure Morphology Oxygen Oxygen content Physical Sciences Physiological effects Physiology Plasma Preservation, Biological - methods Research and Analysis Methods Tight Junctions - metabolism Tight Junctions - ultrastructure Time dependent analysis |
| title | Persistent effectivity of gas plasma-treated, long time-stored liquid on epithelial cell adhesion capacity and membrane morphology |
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