Congenital cytomegalovirus, parvovirus and enterovirus infection in Mozambican newborns at birth: A cross-sectional survey
Congenital cytomegalovirus (cCMV) infection is the most prevalent congenital infection acquired worldwide, with higher incidence in developing countries and among HIV-exposed children. Less is known regarding vertical transmission of parvovirus B19 (B19V) and enterovirus (EV). We aimed to assess the...
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| Veröffentlicht in: | PloS one 2018-03, Vol.13 (3), p.e0194186-e0194186 |
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| creator | Madrid, Lola Varo, Rosauro Maculuve, Sonia Nhampossa, Tacilta Muñoz-Almagro, Carmen Calderón, Enrique J Esteva, Cristina Carrilho, Carla Ismail, Mamudo Vieites, Begoña Friaza, Vicente Lozano-Dominguez, María Del Carmen Menéndez, Clara Bassat, Quique |
| description | Congenital cytomegalovirus (cCMV) infection is the most prevalent congenital infection acquired worldwide, with higher incidence in developing countries and among HIV-exposed children. Less is known regarding vertical transmission of parvovirus B19 (B19V) and enterovirus (EV). We aimed to assess the prevalence of CMV, B19V and EV vertical transmission and compare results of screening of congenital CMV obtained from two different specimens in a semirural Mozambican maternity.
A cross sectional study was conducted among pregnant mothers attending Manhiça District Hospital upon delivery. Information on maternal risk factors was ascertained. Dried umbilical cord (DUC) samples were collected in filter paper for CMV, B19V and EV detection by real-time polymerase chain reaction (RT-PCR), and nasopharyngeal aspirates (NPA) to test for CMV by RT-PCR. Maternal blood samples and placental biopsy samples were also obtained to investigate CMV maternal serology, HIV status and immunopathology.
From September 2014 to January 2015, 118 mothers/newborn pairs were recruited. Prevalence of maternal HIV infection was 31.4% (37/118). CMV RT-PCR was positive in 3/115 (2.6%) of DUC samples and in 3/96 (6.3%) of NPA samples obtained from neonates. The concordance of the RT-PCR assay through DUC with their correspondent NPA sample was moderate (Kappa = 0.42 and p |
| doi_str_mv | 10.1371/journal.pone.0194186 |
| format | Article |
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A cross sectional study was conducted among pregnant mothers attending Manhiça District Hospital upon delivery. Information on maternal risk factors was ascertained. Dried umbilical cord (DUC) samples were collected in filter paper for CMV, B19V and EV detection by real-time polymerase chain reaction (RT-PCR), and nasopharyngeal aspirates (NPA) to test for CMV by RT-PCR. Maternal blood samples and placental biopsy samples were also obtained to investigate CMV maternal serology, HIV status and immunopathology.
From September 2014 to January 2015, 118 mothers/newborn pairs were recruited. Prevalence of maternal HIV infection was 31.4% (37/118). CMV RT-PCR was positive in 3/115 (2.6%) of DUC samples and in 3/96 (6.3%) of NPA samples obtained from neonates. The concordance of the RT-PCR assay through DUC with their correspondent NPA sample was moderate (Kappa = 0.42 and p<0.001. No differences on cCMV prevalence were found among HIV-exposed and unexposed. All (100%) mothers were seropositive for CMV IgG. RT-PCR of EV and B19V in DUC were both negative in all screened cases. No histological specific findings were found in placental tissues. No risk factors associated to vertical transmission of these viral infections were found.
This study indicates the significant occurrence of vertical transmission of CMV in southern Mozambique. Larger studies are needed to evaluate the true burden, clinical relevance and consequences of congenital infections with such pathogens in resource-constrained settings.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0194186</identifier><identifier>PMID: 29538464</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology and Life Sciences ; Biopsy ; Children ; Citomegalovirus ; Congenital diseases ; Congenital infection ; Cross-sectional studies ; Cytomegalovirus ; Cytomegaloviruses ; Developing countries ; Disease transmission ; Enterovirus infections ; Enteroviruses ; Filter paper ; Health aspects ; HIV ; Hospitals ; Human immunodeficiency virus ; Immunoglobulin G ; Infants nadons ; Infections ; Infectious diseases ; LDCs ; Medicine and Health Sciences ; Neonates ; Newborn babies ; Newborn infants ; Parvovirus infections ; Parvoviruses ; Pathology ; Placenta ; Polymerase chain reaction ; Pregnancy ; Research and Analysis Methods ; Risk analysis ; Risk factors ; Serology ; Tissues ; Umbilical cord ; Viral infections ; Womens health</subject><ispartof>PloS one, 2018-03, Vol.13 (3), p.e0194186-e0194186</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Madrid et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>cc by (c) Madrid et al., 2018 info:eu-repo/semantics/openAccess <a href="http://creativecommons.org/licenses/by/3.0/es/">http://creativecommons.org/licenses/by/3.0/es/</a></rights><rights>2018 Madrid et al 2018 Madrid et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c734t-f56864e8a1d11d8cc157086d665be7657d73d35adbe15eb2b529845e6c603ca93</citedby><cites>FETCH-LOGICAL-c734t-f56864e8a1d11d8cc157086d665be7657d73d35adbe15eb2b529845e6c603ca93</cites><orcidid>0000-0003-4034-6033</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851632/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851632/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,26951,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29538464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gantt, Soren</contributor><creatorcontrib>Madrid, Lola</creatorcontrib><creatorcontrib>Varo, Rosauro</creatorcontrib><creatorcontrib>Maculuve, Sonia</creatorcontrib><creatorcontrib>Nhampossa, Tacilta</creatorcontrib><creatorcontrib>Muñoz-Almagro, Carmen</creatorcontrib><creatorcontrib>Calderón, Enrique J</creatorcontrib><creatorcontrib>Esteva, Cristina</creatorcontrib><creatorcontrib>Carrilho, Carla</creatorcontrib><creatorcontrib>Ismail, Mamudo</creatorcontrib><creatorcontrib>Vieites, Begoña</creatorcontrib><creatorcontrib>Friaza, Vicente</creatorcontrib><creatorcontrib>Lozano-Dominguez, María Del Carmen</creatorcontrib><creatorcontrib>Menéndez, Clara</creatorcontrib><creatorcontrib>Bassat, Quique</creatorcontrib><title>Congenital cytomegalovirus, parvovirus and enterovirus infection in Mozambican newborns at birth: A cross-sectional survey</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Congenital cytomegalovirus (cCMV) infection is the most prevalent congenital infection acquired worldwide, with higher incidence in developing countries and among HIV-exposed children. Less is known regarding vertical transmission of parvovirus B19 (B19V) and enterovirus (EV). We aimed to assess the prevalence of CMV, B19V and EV vertical transmission and compare results of screening of congenital CMV obtained from two different specimens in a semirural Mozambican maternity.
A cross sectional study was conducted among pregnant mothers attending Manhiça District Hospital upon delivery. Information on maternal risk factors was ascertained. Dried umbilical cord (DUC) samples were collected in filter paper for CMV, B19V and EV detection by real-time polymerase chain reaction (RT-PCR), and nasopharyngeal aspirates (NPA) to test for CMV by RT-PCR. Maternal blood samples and placental biopsy samples were also obtained to investigate CMV maternal serology, HIV status and immunopathology.
From September 2014 to January 2015, 118 mothers/newborn pairs were recruited. Prevalence of maternal HIV infection was 31.4% (37/118). CMV RT-PCR was positive in 3/115 (2.6%) of DUC samples and in 3/96 (6.3%) of NPA samples obtained from neonates. The concordance of the RT-PCR assay through DUC with their correspondent NPA sample was moderate (Kappa = 0.42 and p<0.001. No differences on cCMV prevalence were found among HIV-exposed and unexposed. All (100%) mothers were seropositive for CMV IgG. RT-PCR of EV and B19V in DUC were both negative in all screened cases. No histological specific findings were found in placental tissues. No risk factors associated to vertical transmission of these viral infections were found.
This study indicates the significant occurrence of vertical transmission of CMV in southern Mozambique. Larger studies are needed to evaluate the true burden, clinical relevance and consequences of congenital infections with such pathogens in resource-constrained settings.</description><subject>Biology and Life Sciences</subject><subject>Biopsy</subject><subject>Children</subject><subject>Citomegalovirus</subject><subject>Congenital diseases</subject><subject>Congenital infection</subject><subject>Cross-sectional studies</subject><subject>Cytomegalovirus</subject><subject>Cytomegaloviruses</subject><subject>Developing countries</subject><subject>Disease transmission</subject><subject>Enterovirus infections</subject><subject>Enteroviruses</subject><subject>Filter paper</subject><subject>Health aspects</subject><subject>HIV</subject><subject>Hospitals</subject><subject>Human immunodeficiency virus</subject><subject>Immunoglobulin G</subject><subject>Infants nadons</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>LDCs</subject><subject>Medicine and Health Sciences</subject><subject>Neonates</subject><subject>Newborn babies</subject><subject>Newborn infants</subject><subject>Parvovirus infections</subject><subject>Parvoviruses</subject><subject>Pathology</subject><subject>Placenta</subject><subject>Polymerase chain reaction</subject><subject>Pregnancy</subject><subject>Research and Analysis Methods</subject><subject>Risk analysis</subject><subject>Risk factors</subject><subject>Serology</subject><subject>Tissues</subject><subject>Umbilical cord</subject><subject>Viral infections</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><sourceid>XX2</sourceid><sourceid>DOA</sourceid><recordid>eNqNU2uL1DAULaK46-o_EC0IouCMSfNo6wdhGHwMrCz4-hrS9M5MhjYZk3R09teb7nSHqewHKaE36Tnn3p7cmyRPMZpikuO3G9s5I5vp1hqYIlxSXPB7yTkuSTbhGSL3T-Kz5JH3G4QYKTh_mJxlZYwop-fJ9dyaFRgdZJOqfbAtrGRjd9p1_k26lW53iFNp6hRMADfstVmCCtqaGKVf7LVsK62kSQ38rqwzkRDSSruwfpfOUuWs9xN_IMREvnM72D9OHixl4-HJ8L5Ifnz88H3-eXJ59Wkxn11OVE5omCwZLziFQuIa47pQCrMcFbzmnFWQc5bXOakJk3UFmEGVVSwrC8qAK46IkiW5SJ4fdLeN9WKwzYsMYYozwmkREYsDorZyI7ZOt9LthZVa3BxYtxLSBa0aELTEhSSqwjynNFZWZpjGpEyVwHOk6qj1fsjWVS3UKprmZDMSHX8xei1WdidYwTAnWRTABwHlOyUcKHBKhhvicdOvDOWZICh6gSPn1ZDU2V8d-CBa7RU0jTRgu-FfY72sl3_xD_RuRwZU7AYQ8bJtrFX1omLGCEaxlQoWUdM7UPGpodUq9uVSx_MR4fWIEDEB_oSV7LwXi29f_x979XOMfXmCXYNswtrbpuv7zY-BdDC3b0kHy-PFYCT6sbp1Q_RjJYaxirRnp5d6JN3OEfkLtvUd8Q</recordid><startdate>20180314</startdate><enddate>20180314</enddate><creator>Madrid, 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cytomegalovirus, parvovirus and enterovirus infection in Mozambican newborns at birth: A cross-sectional survey</title><author>Madrid, Lola ; Varo, Rosauro ; Maculuve, Sonia ; Nhampossa, Tacilta ; Muñoz-Almagro, Carmen ; Calderón, Enrique J ; Esteva, Cristina ; Carrilho, Carla ; Ismail, Mamudo ; Vieites, Begoña ; Friaza, Vicente ; Lozano-Dominguez, María Del Carmen ; Menéndez, Clara ; Bassat, Quique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c734t-f56864e8a1d11d8cc157086d665be7657d73d35adbe15eb2b529845e6c603ca93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biology and Life Sciences</topic><topic>Biopsy</topic><topic>Children</topic><topic>Citomegalovirus</topic><topic>Congenital diseases</topic><topic>Congenital infection</topic><topic>Cross-sectional studies</topic><topic>Cytomegalovirus</topic><topic>Cytomegaloviruses</topic><topic>Developing 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Mozambican newborns at birth: A cross-sectional survey</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-03-14</date><risdate>2018</risdate><volume>13</volume><issue>3</issue><spage>e0194186</spage><epage>e0194186</epage><pages>e0194186-e0194186</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Congenital cytomegalovirus (cCMV) infection is the most prevalent congenital infection acquired worldwide, with higher incidence in developing countries and among HIV-exposed children. Less is known regarding vertical transmission of parvovirus B19 (B19V) and enterovirus (EV). We aimed to assess the prevalence of CMV, B19V and EV vertical transmission and compare results of screening of congenital CMV obtained from two different specimens in a semirural Mozambican maternity.
A cross sectional study was conducted among pregnant mothers attending Manhiça District Hospital upon delivery. Information on maternal risk factors was ascertained. Dried umbilical cord (DUC) samples were collected in filter paper for CMV, B19V and EV detection by real-time polymerase chain reaction (RT-PCR), and nasopharyngeal aspirates (NPA) to test for CMV by RT-PCR. Maternal blood samples and placental biopsy samples were also obtained to investigate CMV maternal serology, HIV status and immunopathology.
From September 2014 to January 2015, 118 mothers/newborn pairs were recruited. Prevalence of maternal HIV infection was 31.4% (37/118). CMV RT-PCR was positive in 3/115 (2.6%) of DUC samples and in 3/96 (6.3%) of NPA samples obtained from neonates. The concordance of the RT-PCR assay through DUC with their correspondent NPA sample was moderate (Kappa = 0.42 and p<0.001. No differences on cCMV prevalence were found among HIV-exposed and unexposed. All (100%) mothers were seropositive for CMV IgG. RT-PCR of EV and B19V in DUC were both negative in all screened cases. No histological specific findings were found in placental tissues. No risk factors associated to vertical transmission of these viral infections were found.
This study indicates the significant occurrence of vertical transmission of CMV in southern Mozambique. Larger studies are needed to evaluate the true burden, clinical relevance and consequences of congenital infections with such pathogens in resource-constrained settings.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29538464</pmid><doi>10.1371/journal.pone.0194186</doi><tpages>e0194186</tpages><orcidid>https://orcid.org/0000-0003-4034-6033</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-03, Vol.13 (3), p.e0194186-e0194186 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2014123648 |
| source | Recercat; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) Journals Open Acc |
| subjects | Biology and Life Sciences Biopsy Children Citomegalovirus Congenital diseases Congenital infection Cross-sectional studies Cytomegalovirus Cytomegaloviruses Developing countries Disease transmission Enterovirus infections Enteroviruses Filter paper Health aspects HIV Hospitals Human immunodeficiency virus Immunoglobulin G Infants nadons Infections Infectious diseases LDCs Medicine and Health Sciences Neonates Newborn babies Newborn infants Parvovirus infections Parvoviruses Pathology Placenta Polymerase chain reaction Pregnancy Research and Analysis Methods Risk analysis Risk factors Serology Tissues Umbilical cord Viral infections Womens health |
| title | Congenital cytomegalovirus, parvovirus and enterovirus infection in Mozambican newborns at birth: A cross-sectional survey |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T04%3A05%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Congenital%20cytomegalovirus,%20parvovirus%20and%20enterovirus%20infection%20in%20Mozambican%20newborns%20at%20birth:%20A%20cross-sectional%20survey&rft.jtitle=PloS%20one&rft.au=Madrid,%20Lola&rft.date=2018-03-14&rft.volume=13&rft.issue=3&rft.spage=e0194186&rft.epage=e0194186&rft.pages=e0194186-e0194186&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0194186&rft_dat=%3Cgale_plos_%3EA531000585%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2014123648&rft_id=info:pmid/29538464&rft_galeid=A531000585&rft_doaj_id=oai_doaj_org_article_4918a3cb167445689214eb25c9e670cd&rfr_iscdi=true |