Phosphatidylcholine alteration identified using MALDI imaging MS in HBV-infected mouse livers and virus-mediated regeneration defects
In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection and liver regeneration in a mouse model. The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and i...
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| description | In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection and liver regeneration in a mouse model. The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and impairs liver regeneration. As there are very few reports available in the literature on lipid alterations by HBV infection or HBV-mediated liver injury, we have analyzed phospholipids that have important roles in liver regeneration by using matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS) in the livers of HBV model mice. As a result, we identified different phosphatidylcholines (PCs) showing significant changes in their composition as well as cationized ion adduct formation in HBV-infected mouse livers which are associated with virus-mediated regeneration defects. To find the factor of altered PCs, the expression kinetics of enzymes was also examined that regulate PC biosynthesis during liver regeneration. It is noteworthy that the expression of choline-phosphate cytidylyltransferase A (PCYT1A) was significantly delayed in wild type HBV-expressing livers. Moreover, the amount of hepatic total PC was also significantly decreased in wt HBV-expressing mice. These results suggest that infection of HBV alters the composition of PCs which may involve in HBV-mediated regeneration defects and liver disease. |
| doi_str_mv | 10.1371/journal.pone.0103955 |
| format | Article |
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The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and impairs liver regeneration. As there are very few reports available in the literature on lipid alterations by HBV infection or HBV-mediated liver injury, we have analyzed phospholipids that have important roles in liver regeneration by using matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS) in the livers of HBV model mice. As a result, we identified different phosphatidylcholines (PCs) showing significant changes in their composition as well as cationized ion adduct formation in HBV-infected mouse livers which are associated with virus-mediated regeneration defects. To find the factor of altered PCs, the expression kinetics of enzymes was also examined that regulate PC biosynthesis during liver regeneration. It is noteworthy that the expression of choline-phosphate cytidylyltransferase A (PCYT1A) was significantly delayed in wild type HBV-expressing livers. Moreover, the amount of hepatic total PC was also significantly decreased in wt HBV-expressing mice. These results suggest that infection of HBV alters the composition of PCs which may involve in HBV-mediated regeneration defects and liver disease.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0103955</identifier><identifier>PMID: 25101682</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antigens ; Autophagy ; Biology and Life Sciences ; Biosynthesis ; Cancer ; Cell cycle ; Cell division ; Choline ; Choline-phosphate cytidylyltransferase ; Defects ; Deoxyribonucleic acid ; DNA ; Enzymes ; Growth factors ; Health aspects ; Hepatectomy ; Hepatitis ; Hepatitis B ; Hepatitis B - complications ; Hepatitis B - physiopathology ; Hepatitis B virus ; Homeostasis ; Infection ; Infections ; Injury analysis ; Ionization ; Ischemia ; Kinetics ; Lecithin ; Lipid metabolism ; Lipids ; Liver ; Liver - physiopathology ; Liver - virology ; Liver diseases ; Liver Regeneration ; Male ; Mass spectrometry ; Mass spectroscopy ; Medical research ; Medicine ; Medicine and Health Sciences ; Metabolism ; Metabolites ; Mice ; Mice, Inbred BALB C ; Mouse devices ; Pharmacology ; Phosphatidylcholine ; Phosphatidylcholines - chemistry ; Phosphatidylcholines - metabolism ; Phospholipids ; Phospholipids - metabolism ; Plasma ; Principal Component Analysis ; Regeneration ; Rodents ; Scientific imaging ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tumor necrosis factor-TNF ; Viruses</subject><ispartof>PloS one, 2014-08, Vol.9 (8), p.e103955-e103955</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Park et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Park et al 2014 Park et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ea292f889f633c50b1f642b18b97ca6bc22fbc62deb0c46eeeef4c2e741c4c6d3</citedby><cites>FETCH-LOGICAL-c692t-ea292f889f633c50b1f642b18b97ca6bc22fbc62deb0c46eeeef4c2e741c4c6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125171/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4125171/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25101682$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Eun-Sook</creatorcontrib><creatorcontrib>Lee, Jeong Hwa</creatorcontrib><creatorcontrib>Hong, Ji Hye</creatorcontrib><creatorcontrib>Park, Yong Kwang</creatorcontrib><creatorcontrib>Lee, Joon Won</creatorcontrib><creatorcontrib>Lee, Won-Jae</creatorcontrib><creatorcontrib>Lee, Jae Won</creatorcontrib><creatorcontrib>Kim, Kwang Pyo</creatorcontrib><creatorcontrib>Kim, Kyun-Hwan</creatorcontrib><title>Phosphatidylcholine alteration identified using MALDI imaging MS in HBV-infected mouse livers and virus-mediated regeneration defects</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection and liver regeneration in a mouse model. The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and impairs liver regeneration. As there are very few reports available in the literature on lipid alterations by HBV infection or HBV-mediated liver injury, we have analyzed phospholipids that have important roles in liver regeneration by using matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS) in the livers of HBV model mice. As a result, we identified different phosphatidylcholines (PCs) showing significant changes in their composition as well as cationized ion adduct formation in HBV-infected mouse livers which are associated with virus-mediated regeneration defects. To find the factor of altered PCs, the expression kinetics of enzymes was also examined that regulate PC biosynthesis during liver regeneration. It is noteworthy that the expression of choline-phosphate cytidylyltransferase A (PCYT1A) was significantly delayed in wild type HBV-expressing livers. Moreover, the amount of hepatic total PC was also significantly decreased in wt HBV-expressing mice. These results suggest that infection of HBV alters the composition of PCs which may involve in HBV-mediated regeneration defects and liver disease.</description><subject>Animals</subject><subject>Antigens</subject><subject>Autophagy</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Cancer</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Choline</subject><subject>Choline-phosphate cytidylyltransferase</subject><subject>Defects</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Enzymes</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Hepatectomy</subject><subject>Hepatitis</subject><subject>Hepatitis B</subject><subject>Hepatitis B - complications</subject><subject>Hepatitis B - physiopathology</subject><subject>Hepatitis B virus</subject><subject>Homeostasis</subject><subject>Infection</subject><subject>Infections</subject><subject>Injury analysis</subject><subject>Ionization</subject><subject>Ischemia</subject><subject>Kinetics</subject><subject>Lecithin</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Liver</subject><subject>Liver - physiopathology</subject><subject>Liver - virology</subject><subject>Liver diseases</subject><subject>Liver Regeneration</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mouse devices</subject><subject>Pharmacology</subject><subject>Phosphatidylcholine</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phospholipids</subject><subject>Phospholipids - metabolism</subject><subject>Plasma</subject><subject>Principal Component Analysis</subject><subject>Regeneration</subject><subject>Rodents</subject><subject>Scientific imaging</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Tumor necrosis factor-TNF</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1tv0zAUxyMEYmPwDRBEQkLwkOJbbi9IZVxWqWiIwV4txz5JPaV2sZOKfQC-N06bTg3aA86DnePf-VvnFkXPMZphmuN3N7Z3RrSzjTUwQxjRMk0fRKe4pCTJCKIPj84n0RPvbxBKaZFlj6MTkmKEs4KcRn--razfrESn1W0rV7bVBmLRduCCyZpYKzCdrjWouPfaNPHX-fLjItZr0ez-rmJt4osP14k2NcguYGvbe4hbvQXnY2FUvNWu98kalBbDvYMGzEFeweDln0aPatF6eDbuZ9HPz59-nF8ky8svi_P5MpFZSboEBClJXRRlnVEqU1ThOmOkwkVV5lJklSSkrmRGFFRIsgzCqpkkkDMsmcwUPYte7nU3rfV8zKDnBGGGKMpJGYjFnlBW3PCNC4G6W26F5juDdQ0XrtOyBa4UQhWlFeBCMSXLohKVxBUWqBCM7rTej6_1VQhfhkw60U5EpzdGr3hjt5zhUKAcB4E3o4Czv3rwHV9rL6FthYGQZY7TlNCszFMW0Ff_oPdHN1KNCAGEktnwrhxE-ZzhIkdFSopAze6hwqdgrWVot1oH-8Th7cQhMB387hrRe88XV9__n728nrKvj9gVhL5cedv2Q-v4Kcj2oHTWewf1XZIx4sO0HLLBh2nh47QEtxfHBbpzOowH_QuG0hIu</recordid><startdate>20140807</startdate><enddate>20140807</enddate><creator>Park, 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alteration identified using MALDI imaging MS in HBV-infected mouse livers and virus-mediated regeneration defects</title><author>Park, Eun-Sook ; Lee, Jeong Hwa ; Hong, Ji Hye ; Park, Yong Kwang ; Lee, Joon Won ; Lee, Won-Jae ; Lee, Jae Won ; Kim, Kwang Pyo ; Kim, Kyun-Hwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-ea292f889f633c50b1f642b18b97ca6bc22fbc62deb0c46eeeef4c2e741c4c6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Autophagy</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Cancer</topic><topic>Cell cycle</topic><topic>Cell division</topic><topic>Choline</topic><topic>Choline-phosphate cytidylyltransferase</topic><topic>Defects</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Enzymes</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Hepatectomy</topic><topic>Hepatitis</topic><topic>Hepatitis B</topic><topic>Hepatitis B - complications</topic><topic>Hepatitis B - physiopathology</topic><topic>Hepatitis B virus</topic><topic>Homeostasis</topic><topic>Infection</topic><topic>Infections</topic><topic>Injury analysis</topic><topic>Ionization</topic><topic>Ischemia</topic><topic>Kinetics</topic><topic>Lecithin</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Liver</topic><topic>Liver - physiopathology</topic><topic>Liver - virology</topic><topic>Liver diseases</topic><topic>Liver Regeneration</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mouse devices</topic><topic>Pharmacology</topic><topic>Phosphatidylcholine</topic><topic>Phosphatidylcholines - chemistry</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phospholipids</topic><topic>Phospholipids - metabolism</topic><topic>Plasma</topic><topic>Principal Component Analysis</topic><topic>Regeneration</topic><topic>Rodents</topic><topic>Scientific imaging</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Tumor necrosis factor-TNF</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Eun-Sook</creatorcontrib><creatorcontrib>Lee, Jeong Hwa</creatorcontrib><creatorcontrib>Hong, Ji Hye</creatorcontrib><creatorcontrib>Park, Yong Kwang</creatorcontrib><creatorcontrib>Lee, Joon Won</creatorcontrib><creatorcontrib>Lee, Won-Jae</creatorcontrib><creatorcontrib>Lee, Jae Won</creatorcontrib><creatorcontrib>Kim, Kwang Pyo</creatorcontrib><creatorcontrib>Kim, 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Pyo</au><au>Kim, Kyun-Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphatidylcholine alteration identified using MALDI imaging MS in HBV-infected mouse livers and virus-mediated regeneration defects</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-08-07</date><risdate>2014</risdate><volume>9</volume><issue>8</issue><spage>e103955</spage><epage>e103955</epage><pages>e103955-e103955</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection and liver regeneration in a mouse model. The liver controls lipid biogenesis and bile acid homeostasis. Infection of HBV causes various liver diseases and impairs liver regeneration. As there are very few reports available in the literature on lipid alterations by HBV infection or HBV-mediated liver injury, we have analyzed phospholipids that have important roles in liver regeneration by using matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS) in the livers of HBV model mice. As a result, we identified different phosphatidylcholines (PCs) showing significant changes in their composition as well as cationized ion adduct formation in HBV-infected mouse livers which are associated with virus-mediated regeneration defects. To find the factor of altered PCs, the expression kinetics of enzymes was also examined that regulate PC biosynthesis during liver regeneration. It is noteworthy that the expression of choline-phosphate cytidylyltransferase A (PCYT1A) was significantly delayed in wild type HBV-expressing livers. Moreover, the amount of hepatic total PC was also significantly decreased in wt HBV-expressing mice. These results suggest that infection of HBV alters the composition of PCs which may involve in HBV-mediated regeneration defects and liver disease.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25101682</pmid><doi>10.1371/journal.pone.0103955</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-08, Vol.9 (8), p.e103955-e103955 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2014030729 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Antigens Autophagy Biology and Life Sciences Biosynthesis Cancer Cell cycle Cell division Choline Choline-phosphate cytidylyltransferase Defects Deoxyribonucleic acid DNA Enzymes Growth factors Health aspects Hepatectomy Hepatitis Hepatitis B Hepatitis B - complications Hepatitis B - physiopathology Hepatitis B virus Homeostasis Infection Infections Injury analysis Ionization Ischemia Kinetics Lecithin Lipid metabolism Lipids Liver Liver - physiopathology Liver - virology Liver diseases Liver Regeneration Male Mass spectrometry Mass spectroscopy Medical research Medicine Medicine and Health Sciences Metabolism Metabolites Mice Mice, Inbred BALB C Mouse devices Pharmacology Phosphatidylcholine Phosphatidylcholines - chemistry Phosphatidylcholines - metabolism Phospholipids Phospholipids - metabolism Plasma Principal Component Analysis Regeneration Rodents Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tumor necrosis factor-TNF Viruses |
| title | Phosphatidylcholine alteration identified using MALDI imaging MS in HBV-infected mouse livers and virus-mediated regeneration defects |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T11%3A13%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phosphatidylcholine%20alteration%20identified%20using%20MALDI%20imaging%20MS%20in%20HBV-infected%20mouse%20livers%20and%20virus-mediated%20regeneration%20defects&rft.jtitle=PloS%20one&rft.au=Park,%20Eun-Sook&rft.date=2014-08-07&rft.volume=9&rft.issue=8&rft.spage=e103955&rft.epage=e103955&rft.pages=e103955-e103955&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0103955&rft_dat=%3Cgale_plos_%3EA418708528%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2014030729&rft_id=info:pmid/25101682&rft_galeid=A418708528&rft_doaj_id=oai_doaj_org_article_dd00b33be18d4dc98babc1b1a08a4329&rfr_iscdi=true |