A FRET-based biosensor for measuring Gα13 activation in single cells

Förster Resonance Energy Transfer (FRET) provides a way to directly observe the activation of heterotrimeric G-proteins by G-protein coupled receptors (GPCRs). To this end, FRET based biosensors are made, employing heterotrimeric G-protein subunits tagged with fluorescent proteins. These FRET based...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2018-03, Vol.13 (3), p.e0193705-e0193705
Hauptverfasser:	Mastop, Marieke, Reinhard, Nathalie R, Zuconelli, Cristiane R, Terwey, Fenna, Gadella, Jr, Theodorus W J, van Unen, Jakobus, Adjobo-Hermans, Merel J W, Goedhart, Joachim
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biology and Life Sciences Biosensors Cellular biology Complement activation Construction standards Endothelial cells Energy measurement Energy transfer Engineering and Technology Fluorescence Fluorescence resonance energy transfer G protein-coupled receptors Gadella Life sciences Localization Microscopy Proteins Receptors Research and Analysis Methods Thrombin
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!