Novel animal model for Achilles tendinopathy: Controlled experimental study of serial injections of collagenase in rabbits
Our goal was to develop a novel technique for inducing Achilles tendinopathy in animal models which more accurately represents the progressive histological and biomechanical characteristic of chronic Achilles tendinopathy in humans. In this animal research study, forty-five rabbits were randomly ass...
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| creator | de Cesar Netto, Cesar Godoy-Santos, Alexandre Leme Augusto Pontin, Pedro Natalino, Renato Jose Mendonça Pereira, Cesar Augusto Martins Lima, Francisco Diego de Oliveira da Fonseca, Lucas Furtado Staggers, Jackson Rucker Cavinatto, Leonardo Muntada Schon, Lew Charles de Camargo, Olavo Pires Fernandes, Túlio Diniz |
| description | Our goal was to develop a novel technique for inducing Achilles tendinopathy in animal models which more accurately represents the progressive histological and biomechanical characteristic of chronic Achilles tendinopathy in humans. In this animal research study, forty-five rabbits were randomly assigned to three groups and given bilateral Achilles injections. Low dose (LD group) (n = 18) underwent a novel technique with three low-dose (0.1mg) injections of collagenase that were separated by two weeks, the high dose group (HD) (n = 18) underwent traditional single high-dose (0.3mg) injections, and the third group were controls (n = 9). Six rabbits were sacrificed from each experimental group (LD and HD) at 10, 12 and 16 weeks. Control animals were sacrificed after 16 weeks. Histological and biomechanical properties were then compared in all three groups. At 10 weeks, Bonar score and tendon cross sectional area was highest in HD group, with impaired biomechanical properties compared to LD group. At 12 weeks, Bonar score was higher in LD group, with similar biomechanical findings when compared to HD group. After 16 weeks, Bonar score was significantly increased for both LD group (11,8±2,28) and HD group (5,6±2,51), when compared to controls (2±0,76). LD group showed more pronounced histological and biomechanical findings, including cross sectional area of the tendon, Young's modulus, yield stress and ultimate tensile strength. In conclusion, Achilles tendinopathy in animal models that were induced by serial injections of low-dose collagenase showed more pronounced histological and biomechanical findings after 16 weeks than traditional techniques, mimicking better the progressive and chronic characteristic of the tendinopathy in humans. |
| doi_str_mv | 10.1371/journal.pone.0192769 |
| format | Article |
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In this animal research study, forty-five rabbits were randomly assigned to three groups and given bilateral Achilles injections. Low dose (LD group) (n = 18) underwent a novel technique with three low-dose (0.1mg) injections of collagenase that were separated by two weeks, the high dose group (HD) (n = 18) underwent traditional single high-dose (0.3mg) injections, and the third group were controls (n = 9). Six rabbits were sacrificed from each experimental group (LD and HD) at 10, 12 and 16 weeks. Control animals were sacrificed after 16 weeks. Histological and biomechanical properties were then compared in all three groups. At 10 weeks, Bonar score and tendon cross sectional area was highest in HD group, with impaired biomechanical properties compared to LD group. At 12 weeks, Bonar score was higher in LD group, with similar biomechanical findings when compared to HD group. After 16 weeks, Bonar score was significantly increased for both LD group (11,8±2,28) and HD group (5,6±2,51), when compared to controls (2±0,76). LD group showed more pronounced histological and biomechanical findings, including cross sectional area of the tendon, Young's modulus, yield stress and ultimate tensile strength. In conclusion, Achilles tendinopathy in animal models that were induced by serial injections of low-dose collagenase showed more pronounced histological and biomechanical findings after 16 weeks than traditional techniques, mimicking better the progressive and chronic characteristic of the tendinopathy in humans.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0192769</identifier><identifier>PMID: 29438431</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Achilles tendon ; Achilles Tendon - pathology ; Animal models ; Animal research ; Animals ; Ankle ; Biology and Life Sciences ; Biomechanical Phenomena ; Biomechanics ; Collagen ; Collagenase ; Collagenases - administration & dosage ; Development and progression ; Disease Models, Animal ; Female ; Growth factors ; Health aspects ; Injuries ; Mechanical properties ; Medicine ; Medicine and Health Sciences ; Mimicry ; Modulus of elasticity ; Physical Sciences ; Rabbits ; Research and Analysis Methods ; Surgery ; Tendinitis ; Tendinopathy - chemically induced ; Tendinopathy - pathology ; Tendons ; Ultimate tensile strength ; Yield stress</subject><ispartof>PloS one, 2018-02, Vol.13 (2), p.e0192769-e0192769</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 de Cesar Netto et al. 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In this animal research study, forty-five rabbits were randomly assigned to three groups and given bilateral Achilles injections. Low dose (LD group) (n = 18) underwent a novel technique with three low-dose (0.1mg) injections of collagenase that were separated by two weeks, the high dose group (HD) (n = 18) underwent traditional single high-dose (0.3mg) injections, and the third group were controls (n = 9). Six rabbits were sacrificed from each experimental group (LD and HD) at 10, 12 and 16 weeks. Control animals were sacrificed after 16 weeks. Histological and biomechanical properties were then compared in all three groups. At 10 weeks, Bonar score and tendon cross sectional area was highest in HD group, with impaired biomechanical properties compared to LD group. At 12 weeks, Bonar score was higher in LD group, with similar biomechanical findings when compared to HD group. After 16 weeks, Bonar score was significantly increased for both LD group (11,8±2,28) and HD group (5,6±2,51), when compared to controls (2±0,76). LD group showed more pronounced histological and biomechanical findings, including cross sectional area of the tendon, Young's modulus, yield stress and ultimate tensile strength. In conclusion, Achilles tendinopathy in animal models that were induced by serial injections of low-dose collagenase showed more pronounced histological and biomechanical findings after 16 weeks than traditional techniques, mimicking better the progressive and chronic characteristic of the tendinopathy in humans.</description><subject>Achilles tendon</subject><subject>Achilles Tendon - pathology</subject><subject>Animal models</subject><subject>Animal research</subject><subject>Animals</subject><subject>Ankle</subject><subject>Biology and Life Sciences</subject><subject>Biomechanical Phenomena</subject><subject>Biomechanics</subject><subject>Collagen</subject><subject>Collagenase</subject><subject>Collagenases - administration & dosage</subject><subject>Development and progression</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Injuries</subject><subject>Mechanical properties</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mimicry</subject><subject>Modulus of elasticity</subject><subject>Physical Sciences</subject><subject>Rabbits</subject><subject>Research and Analysis Methods</subject><subject>Surgery</subject><subject>Tendinitis</subject><subject>Tendinopathy - 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In this animal research study, forty-five rabbits were randomly assigned to three groups and given bilateral Achilles injections. Low dose (LD group) (n = 18) underwent a novel technique with three low-dose (0.1mg) injections of collagenase that were separated by two weeks, the high dose group (HD) (n = 18) underwent traditional single high-dose (0.3mg) injections, and the third group were controls (n = 9). Six rabbits were sacrificed from each experimental group (LD and HD) at 10, 12 and 16 weeks. Control animals were sacrificed after 16 weeks. Histological and biomechanical properties were then compared in all three groups. At 10 weeks, Bonar score and tendon cross sectional area was highest in HD group, with impaired biomechanical properties compared to LD group. At 12 weeks, Bonar score was higher in LD group, with similar biomechanical findings when compared to HD group. After 16 weeks, Bonar score was significantly increased for both LD group (11,8±2,28) and HD group (5,6±2,51), when compared to controls (2±0,76). LD group showed more pronounced histological and biomechanical findings, including cross sectional area of the tendon, Young's modulus, yield stress and ultimate tensile strength. In conclusion, Achilles tendinopathy in animal models that were induced by serial injections of low-dose collagenase showed more pronounced histological and biomechanical findings after 16 weeks than traditional techniques, mimicking better the progressive and chronic characteristic of the tendinopathy in humans.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29438431</pmid><doi>10.1371/journal.pone.0192769</doi><tpages>e0192769</tpages><orcidid>https://orcid.org/0000-0001-6037-0685</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2001895430 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Achilles tendon Achilles Tendon - pathology Animal models Animal research Animals Ankle Biology and Life Sciences Biomechanical Phenomena Biomechanics Collagen Collagenase Collagenases - administration & dosage Development and progression Disease Models, Animal Female Growth factors Health aspects Injuries Mechanical properties Medicine Medicine and Health Sciences Mimicry Modulus of elasticity Physical Sciences Rabbits Research and Analysis Methods Surgery Tendinitis Tendinopathy - chemically induced Tendinopathy - pathology Tendons Ultimate tensile strength Yield stress |
| title | Novel animal model for Achilles tendinopathy: Controlled experimental study of serial injections of collagenase in rabbits |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T19%3A35%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20animal%20model%20for%20Achilles%20tendinopathy:%20Controlled%20experimental%20study%20of%20serial%20injections%20of%20collagenase%20in%20rabbits&rft.jtitle=PloS%20one&rft.au=de%20Cesar%20Netto,%20Cesar&rft.date=2018-02-13&rft.volume=13&rft.issue=2&rft.spage=e0192769&rft.epage=e0192769&rft.pages=e0192769-e0192769&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0192769&rft_dat=%3Cgale_plos_%3EA527419830%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2001895430&rft_id=info:pmid/29438431&rft_galeid=A527419830&rft_doaj_id=oai_doaj_org_article_a1b1ffc2a73841c4a984b25c947437fc&rfr_iscdi=true |