Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Acinetobacter baumannii among horses entering a veterinary teaching hospital: The contemporary "Trojan Horse"
Pathogens frequently associated with multi-drug resistant (MDR) phenotypes, including extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and Acinetobacter baumannii isolated from horses admitted to horse clinics, pose a risk for animal patients and personnel in horse clini...
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| Veröffentlicht in: | PloS one 2018-01, Vol.13 (1), p.e0191873-e0191873 |
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| creator | Walther, Birgit Klein, Katja-Sophia Barton, Ann-Kristin Semmler, Torsten Huber, Charlotte Wolf, Silver Anthony Tedin, Karsten Merle, Roswitha Mitrach, Franziska Guenther, Sebastian Lübke-Becker, Antina Gehlen, Heidrun |
| description | Pathogens frequently associated with multi-drug resistant (MDR) phenotypes, including extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and Acinetobacter baumannii isolated from horses admitted to horse clinics, pose a risk for animal patients and personnel in horse clinics. To estimate current rates of colonization, a total of 341 equine patients were screened for carriage of zoonotic indicator pathogens at hospital admission. Horses showing clinical signs associated with colic (n = 233) or open wounds (n = 108) were selected for microbiological examination of nostril swabs, faecal samples and wound swabs taken from the open wound group. The results showed alarming carriage rates of Gram-negative MDR pathogens in equine patients: 10.7% (34 of 318) of validated faecal specimens were positive for ESBL-E (94%: ESBL-producing Escherichia coli), with recorded rates of 10.5% for the colic and 11% for the open wound group. 92.7% of the ESBL-producing E. coli were phenotypically resistant to three or more classes of antimicrobials. A. baumannii was rarely detected (0.9%), and all faecal samples investigated were negative for Salmonella, both directly and after two enrichment steps. Screening results for the equine nostril swabs showed detection rates for ESBL-E of 3.4% among colic patients and 0.9% in the open wound group, with an average rate of 2.6% (9/340) for both indications. For all 41 ESBL-producing E. coli isolated, a broad heterogeneity was revealed using pulsed-field gel electrophoresis (PFGE) patterns and whole genome sequencing (WGS) -analysis. However, a predominance of sequence type complex (STC)10 and STC1250 was observed, including several novel STs. The most common genes associated with ESBL-production were identified as blaCTX-M-1 (31/41; 75.6%) and blaSHV-12 (24.4%). The results of this study reveal a disturbingly large fraction of multi-drug resistant and ESBL-producing E. coli among equine patients, posing a clear threat to established hygiene management systems and work-place safety of veterinary staff in horse clinics. |
| doi_str_mv | 10.1371/journal.pone.0191873 |
| format | Article |
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To estimate current rates of colonization, a total of 341 equine patients were screened for carriage of zoonotic indicator pathogens at hospital admission. Horses showing clinical signs associated with colic (n = 233) or open wounds (n = 108) were selected for microbiological examination of nostril swabs, faecal samples and wound swabs taken from the open wound group. The results showed alarming carriage rates of Gram-negative MDR pathogens in equine patients: 10.7% (34 of 318) of validated faecal specimens were positive for ESBL-E (94%: ESBL-producing Escherichia coli), with recorded rates of 10.5% for the colic and 11% for the open wound group. 92.7% of the ESBL-producing E. coli were phenotypically resistant to three or more classes of antimicrobials. A. baumannii was rarely detected (0.9%), and all faecal samples investigated were negative for Salmonella, both directly and after two enrichment steps. Screening results for the equine nostril swabs showed detection rates for ESBL-E of 3.4% among colic patients and 0.9% in the open wound group, with an average rate of 2.6% (9/340) for both indications. For all 41 ESBL-producing E. coli isolated, a broad heterogeneity was revealed using pulsed-field gel electrophoresis (PFGE) patterns and whole genome sequencing (WGS) -analysis. However, a predominance of sequence type complex (STC)10 and STC1250 was observed, including several novel STs. The most common genes associated with ESBL-production were identified as blaCTX-M-1 (31/41; 75.6%) and blaSHV-12 (24.4%). The results of this study reveal a disturbingly large fraction of multi-drug resistant and ESBL-producing E. coli among equine patients, posing a clear threat to established hygiene management systems and work-place safety of veterinary staff in horse clinics.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0191873</identifier><identifier>PMID: 29381714</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acinetobacter baumannii ; Acinetobacter baumannii - genetics ; Acinetobacter baumannii - metabolism ; Animals ; Antimicrobial agents ; Bacteria ; Beta lactamases ; beta-Lactamases - biosynthesis ; Biology and Life Sciences ; Colic ; Colonization ; Disease control ; Drug resistance ; E coli ; Electrophoresis, Gel, Pulsed-Field ; Enterobacteriaceae ; Epidemiology ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Gel electrophoresis ; Gene sequencing ; Genes, Bacterial ; Genetic aspects ; Genomes ; Genomics ; Horses ; Horses - microbiology ; Hospitals, Animal ; Hospitals, Teaching ; Hygiene ; Management systems ; Medicine and Health Sciences ; Microbiology ; Microscopy ; Multidrug resistance ; Nucleotide sequence ; Pathogens ; Patients ; Physiological aspects ; Pulsed-field gel electrophoresis ; Risk factors ; Salmonella ; Staphylococcus aureus ; Staphylococcus infections ; Studies ; Surgery ; Veterinary medicine ; Wounds ; Zoonoses ; β Lactamase</subject><ispartof>PloS one, 2018-01, Vol.13 (1), p.e0191873-e0191873</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Walther et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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To estimate current rates of colonization, a total of 341 equine patients were screened for carriage of zoonotic indicator pathogens at hospital admission. Horses showing clinical signs associated with colic (n = 233) or open wounds (n = 108) were selected for microbiological examination of nostril swabs, faecal samples and wound swabs taken from the open wound group. The results showed alarming carriage rates of Gram-negative MDR pathogens in equine patients: 10.7% (34 of 318) of validated faecal specimens were positive for ESBL-E (94%: ESBL-producing Escherichia coli), with recorded rates of 10.5% for the colic and 11% for the open wound group. 92.7% of the ESBL-producing E. coli were phenotypically resistant to three or more classes of antimicrobials. A. baumannii was rarely detected (0.9%), and all faecal samples investigated were negative for Salmonella, both directly and after two enrichment steps. Screening results for the equine nostril swabs showed detection rates for ESBL-E of 3.4% among colic patients and 0.9% in the open wound group, with an average rate of 2.6% (9/340) for both indications. For all 41 ESBL-producing E. coli isolated, a broad heterogeneity was revealed using pulsed-field gel electrophoresis (PFGE) patterns and whole genome sequencing (WGS) -analysis. However, a predominance of sequence type complex (STC)10 and STC1250 was observed, including several novel STs. The most common genes associated with ESBL-production were identified as blaCTX-M-1 (31/41; 75.6%) and blaSHV-12 (24.4%). The results of this study reveal a disturbingly large fraction of multi-drug resistant and ESBL-producing E. coli among equine patients, posing a clear threat to established hygiene management systems and work-place safety of veterinary staff in horse clinics.</description><subject>Acinetobacter baumannii</subject><subject>Acinetobacter baumannii - genetics</subject><subject>Acinetobacter baumannii - metabolism</subject><subject>Animals</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Beta lactamases</subject><subject>beta-Lactamases - biosynthesis</subject><subject>Biology and Life Sciences</subject><subject>Colic</subject><subject>Colonization</subject><subject>Disease control</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Enterobacteriaceae</subject><subject>Epidemiology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Gel electrophoresis</subject><subject>Gene sequencing</subject><subject>Genes, Bacterial</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Horses</subject><subject>Horses - microbiology</subject><subject>Hospitals, Animal</subject><subject>Hospitals, Teaching</subject><subject>Hygiene</subject><subject>Management systems</subject><subject>Medicine and Health Sciences</subject><subject>Microbiology</subject><subject>Microscopy</subject><subject>Multidrug resistance</subject><subject>Nucleotide sequence</subject><subject>Pathogens</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Pulsed-field gel electrophoresis</subject><subject>Risk factors</subject><subject>Salmonella</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Studies</subject><subject>Surgery</subject><subject>Veterinary medicine</subject><subject>Wounds</subject><subject>Zoonoses</subject><subject>β 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Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walther, Birgit</au><au>Klein, Katja-Sophia</au><au>Barton, Ann-Kristin</au><au>Semmler, Torsten</au><au>Huber, Charlotte</au><au>Wolf, Silver Anthony</au><au>Tedin, Karsten</au><au>Merle, Roswitha</au><au>Mitrach, Franziska</au><au>Guenther, Sebastian</au><au>Lübke-Becker, Antina</au><au>Gehlen, Heidrun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Acinetobacter baumannii among horses entering a veterinary teaching hospital: The contemporary "Trojan Horse"</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>13</volume><issue>1</issue><spage>e0191873</spage><epage>e0191873</epage><pages>e0191873-e0191873</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pathogens frequently associated with multi-drug resistant (MDR) phenotypes, including extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and Acinetobacter baumannii isolated from horses admitted to horse clinics, pose a risk for animal patients and personnel in horse clinics. To estimate current rates of colonization, a total of 341 equine patients were screened for carriage of zoonotic indicator pathogens at hospital admission. Horses showing clinical signs associated with colic (n = 233) or open wounds (n = 108) were selected for microbiological examination of nostril swabs, faecal samples and wound swabs taken from the open wound group. The results showed alarming carriage rates of Gram-negative MDR pathogens in equine patients: 10.7% (34 of 318) of validated faecal specimens were positive for ESBL-E (94%: ESBL-producing Escherichia coli), with recorded rates of 10.5% for the colic and 11% for the open wound group. 92.7% of the ESBL-producing E. coli were phenotypically resistant to three or more classes of antimicrobials. A. baumannii was rarely detected (0.9%), and all faecal samples investigated were negative for Salmonella, both directly and after two enrichment steps. Screening results for the equine nostril swabs showed detection rates for ESBL-E of 3.4% among colic patients and 0.9% in the open wound group, with an average rate of 2.6% (9/340) for both indications. For all 41 ESBL-producing E. coli isolated, a broad heterogeneity was revealed using pulsed-field gel electrophoresis (PFGE) patterns and whole genome sequencing (WGS) -analysis. However, a predominance of sequence type complex (STC)10 and STC1250 was observed, including several novel STs. The most common genes associated with ESBL-production were identified as blaCTX-M-1 (31/41; 75.6%) and blaSHV-12 (24.4%). The results of this study reveal a disturbingly large fraction of multi-drug resistant and ESBL-producing E. coli among equine patients, posing a clear threat to established hygiene management systems and work-place safety of veterinary staff in horse clinics.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29381714</pmid><doi>10.1371/journal.pone.0191873</doi><orcidid>https://orcid.org/0000-0003-3112-5722</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-01, Vol.13 (1), p.e0191873-e0191873 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1992660435 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acinetobacter baumannii Acinetobacter baumannii - genetics Acinetobacter baumannii - metabolism Animals Antimicrobial agents Bacteria Beta lactamases beta-Lactamases - biosynthesis Biology and Life Sciences Colic Colonization Disease control Drug resistance E coli Electrophoresis, Gel, Pulsed-Field Enterobacteriaceae Epidemiology Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gel electrophoresis Gene sequencing Genes, Bacterial Genetic aspects Genomes Genomics Horses Horses - microbiology Hospitals, Animal Hospitals, Teaching Hygiene Management systems Medicine and Health Sciences Microbiology Microscopy Multidrug resistance Nucleotide sequence Pathogens Patients Physiological aspects Pulsed-field gel electrophoresis Risk factors Salmonella Staphylococcus aureus Staphylococcus infections Studies Surgery Veterinary medicine Wounds Zoonoses β Lactamase |
| title | Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Acinetobacter baumannii among horses entering a veterinary teaching hospital: The contemporary "Trojan Horse" |
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