Phytochemical characterization of the Vochysia rufa (Vochysiaceae) extract and its effects on oxidative stress in the pancreata of streptozotocin-induced diabetic rats
Aqueous extract of macerated Vochysia rufa stem bark has been commonly used in the treatment of diabetes. Therefore, we evaluated the antihyperglycemic and antioxidant effects of an extract of V. rufa on the pancreata of streptozotocin (STZ)-induced diabetic rats. Animals received one of the followi...
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| Veröffentlicht in: | PloS one 2017-10, Vol.12 (10), p.e0184807-e0184807 |
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| creator | de Gouveia, Neire M Rodrigues, Wellington F de Sousa, Raquel M F Calábria, Luciana K Mundim, Antonio V Miguel, Camila B Oliveira, Carlo J F Lazo-Chica, Javier E de Oliveira, Alberto Lago, João H G Dos Santos, Vagner B do Lago, Claudimir L Espindola, Foued S |
| description | Aqueous extract of macerated Vochysia rufa stem bark has been commonly used in the treatment of diabetes. Therefore, we evaluated the antihyperglycemic and antioxidant effects of an extract of V. rufa on the pancreata of streptozotocin (STZ)-induced diabetic rats. Animals received one of the following treatments daily by oral gavage: water (diabetic-control), V. rufa extract (diabetic-V. rufa), or glibenclamide (diabetic-GBD). Total antioxidant capacity; levels of thiobarbituric acid reactive substances, reduced glutathione, and sulfhydryls; and superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities were measured in the pancreas. Biochemical analysis of serum total cholesterol and fractions, triglycerides, creatinine, urea, acid uric, ALP, γ-GT, AST, and ALT was performed, and pancreatic β-cells positive for insulin were evaluated by immunohistochemistry. Rats treated with extract exhibited a decrease in fasting blood glucose compared with levels in diabetic control rats. GPx activity and sulfhydryl levels were significantly lower in diabetic-V. rufa rats compared with those of diabetic-control rats. V. rufa extract acted to normalize the biochemical alterations found in diabetic rats (diabetic-controls), as demonstrated by increases in urea, HDL, ALP, AST, and ALT. Reduction in blood glucose was independent of an increase in insulin. The V. rufa extract was found to be composed of free sugars (inositol, galactose, glucose, mannose, sucrose, arabinose, and ribose) as the main metabolites. Thus, aqueous extract of the stem bark of V. rufa is capable of reducing blood glucose, resulting in an antioxidant effect on the pancreatic tissue of STZ-diabetic rats. |
| doi_str_mv | 10.1371/journal.pone.0184807 |
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Faadiel</contributor><creatorcontrib>de Gouveia, Neire M ; Rodrigues, Wellington F ; de Sousa, Raquel M F ; Calábria, Luciana K ; Mundim, Antonio V ; Miguel, Camila B ; Oliveira, Carlo J F ; Lazo-Chica, Javier E ; de Oliveira, Alberto ; Lago, João H G ; Dos Santos, Vagner B ; do Lago, Claudimir L ; Espindola, Foued S ; Essop, M. Faadiel</creatorcontrib><description>Aqueous extract of macerated Vochysia rufa stem bark has been commonly used in the treatment of diabetes. Therefore, we evaluated the antihyperglycemic and antioxidant effects of an extract of V. rufa on the pancreata of streptozotocin (STZ)-induced diabetic rats. Animals received one of the following treatments daily by oral gavage: water (diabetic-control), V. rufa extract (diabetic-V. rufa), or glibenclamide (diabetic-GBD). Total antioxidant capacity; levels of thiobarbituric acid reactive substances, reduced glutathione, and sulfhydryls; and superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities were measured in the pancreas. Biochemical analysis of serum total cholesterol and fractions, triglycerides, creatinine, urea, acid uric, ALP, γ-GT, AST, and ALT was performed, and pancreatic β-cells positive for insulin were evaluated by immunohistochemistry. Rats treated with extract exhibited a decrease in fasting blood glucose compared with levels in diabetic control rats. GPx activity and sulfhydryl levels were significantly lower in diabetic-V. rufa rats compared with those of diabetic-control rats. V. rufa extract acted to normalize the biochemical alterations found in diabetic rats (diabetic-controls), as demonstrated by increases in urea, HDL, ALP, AST, and ALT. Reduction in blood glucose was independent of an increase in insulin. The V. rufa extract was found to be composed of free sugars (inositol, galactose, glucose, mannose, sucrose, arabinose, and ribose) as the main metabolites. Thus, aqueous extract of the stem bark of V. rufa is capable of reducing blood glucose, resulting in an antioxidant effect on the pancreatic tissue of STZ-diabetic rats.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0184807</identifier><identifier>PMID: 28981518</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Adenosine triphosphatase ; Animals ; Antioxidants ; Antioxidants - pharmacology ; Arabinose ; Bark ; Biochemical analysis ; Biology and Life Sciences ; Blood ; Blood Glucose ; Body Weight - drug effects ; Care and treatment ; Catalase ; Catalase - metabolism ; Chemical properties ; Cholesterol ; Creatinine ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - metabolism ; Enzymes ; Galactose ; Glibenclamide ; Glucose ; Glutathione ; Glutathione - metabolism ; Glutathione peroxidase ; High density lipoprotein ; Immunohistochemistry ; Inositol ; Insulin ; Magnoliopsida ; Male ; Mannose ; Medicine and Health Sciences ; Metabolites ; Oxidative stress ; Oxidative Stress - drug effects ; Pancreas ; Pancreas - drug effects ; Pancreas - metabolism ; Peroxidase ; Physical Sciences ; Phytochemicals ; Phytotherapy ; Plant Extracts - pharmacology ; Rats ; Rats, Wistar ; Ribose ; Rodents ; Sucrose ; Sugar ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Thiobarbituric acid ; Thiobarbituric Acid Reactive Substances - metabolism ; Triglycerides ; Urea ; Vochysiaceae ; Water treatment</subject><ispartof>PloS one, 2017-10, Vol.12 (10), p.e0184807-e0184807</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 de Gouveia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Faadiel</contributor><creatorcontrib>de Gouveia, Neire M</creatorcontrib><creatorcontrib>Rodrigues, Wellington F</creatorcontrib><creatorcontrib>de Sousa, Raquel M F</creatorcontrib><creatorcontrib>Calábria, Luciana K</creatorcontrib><creatorcontrib>Mundim, Antonio V</creatorcontrib><creatorcontrib>Miguel, Camila B</creatorcontrib><creatorcontrib>Oliveira, Carlo J F</creatorcontrib><creatorcontrib>Lazo-Chica, Javier E</creatorcontrib><creatorcontrib>de Oliveira, Alberto</creatorcontrib><creatorcontrib>Lago, João H G</creatorcontrib><creatorcontrib>Dos Santos, Vagner B</creatorcontrib><creatorcontrib>do Lago, Claudimir L</creatorcontrib><creatorcontrib>Espindola, Foued S</creatorcontrib><title>Phytochemical characterization of the Vochysia rufa (Vochysiaceae) extract and its effects on oxidative stress in the pancreata of streptozotocin-induced diabetic rats</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Aqueous extract of macerated Vochysia rufa stem bark has been commonly used in the treatment of diabetes. Therefore, we evaluated the antihyperglycemic and antioxidant effects of an extract of V. rufa on the pancreata of streptozotocin (STZ)-induced diabetic rats. Animals received one of the following treatments daily by oral gavage: water (diabetic-control), V. rufa extract (diabetic-V. rufa), or glibenclamide (diabetic-GBD). Total antioxidant capacity; levels of thiobarbituric acid reactive substances, reduced glutathione, and sulfhydryls; and superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities were measured in the pancreas. Biochemical analysis of serum total cholesterol and fractions, triglycerides, creatinine, urea, acid uric, ALP, γ-GT, AST, and ALT was performed, and pancreatic β-cells positive for insulin were evaluated by immunohistochemistry. Rats treated with extract exhibited a decrease in fasting blood glucose compared with levels in diabetic control rats. GPx activity and sulfhydryl levels were significantly lower in diabetic-V. rufa rats compared with those of diabetic-control rats. V. rufa extract acted to normalize the biochemical alterations found in diabetic rats (diabetic-controls), as demonstrated by increases in urea, HDL, ALP, AST, and ALT. Reduction in blood glucose was independent of an increase in insulin. The V. rufa extract was found to be composed of free sugars (inositol, galactose, glucose, mannose, sucrose, arabinose, and ribose) as the main metabolites. Thus, aqueous extract of the stem bark of V. rufa is capable of reducing blood glucose, resulting in an antioxidant effect on the pancreatic tissue of STZ-diabetic rats.</description><subject>Acids</subject><subject>Adenosine triphosphatase</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Arabinose</subject><subject>Bark</subject><subject>Biochemical analysis</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood Glucose</subject><subject>Body Weight - drug effects</subject><subject>Care and treatment</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Chemical properties</subject><subject>Cholesterol</subject><subject>Creatinine</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Enzymes</subject><subject>Galactose</subject><subject>Glibenclamide</subject><subject>Glucose</subject><subject>Glutathione</subject><subject>Glutathione - metabolism</subject><subject>Glutathione peroxidase</subject><subject>High density lipoprotein</subject><subject>Immunohistochemistry</subject><subject>Inositol</subject><subject>Insulin</subject><subject>Magnoliopsida</subject><subject>Male</subject><subject>Mannose</subject><subject>Medicine and Health Sciences</subject><subject>Metabolites</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Pancreas</subject><subject>Pancreas - drug effects</subject><subject>Pancreas - metabolism</subject><subject>Peroxidase</subject><subject>Physical Sciences</subject><subject>Phytochemicals</subject><subject>Phytotherapy</subject><subject>Plant Extracts - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Ribose</subject><subject>Rodents</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Thiobarbituric acid</subject><subject>Thiobarbituric Acid Reactive Substances - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Gouveia, Neire M</au><au>Rodrigues, Wellington F</au><au>de Sousa, Raquel M F</au><au>Calábria, Luciana K</au><au>Mundim, Antonio V</au><au>Miguel, Camila B</au><au>Oliveira, Carlo J F</au><au>Lazo-Chica, Javier E</au><au>de Oliveira, Alberto</au><au>Lago, João H G</au><au>Dos Santos, Vagner B</au><au>do Lago, Claudimir L</au><au>Espindola, Foued S</au><au>Essop, M. Faadiel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical characterization of the Vochysia rufa (Vochysiaceae) extract and its effects on oxidative stress in the pancreata of streptozotocin-induced diabetic rats</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-10-05</date><risdate>2017</risdate><volume>12</volume><issue>10</issue><spage>e0184807</spage><epage>e0184807</epage><pages>e0184807-e0184807</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Aqueous extract of macerated Vochysia rufa stem bark has been commonly used in the treatment of diabetes. Therefore, we evaluated the antihyperglycemic and antioxidant effects of an extract of V. rufa on the pancreata of streptozotocin (STZ)-induced diabetic rats. Animals received one of the following treatments daily by oral gavage: water (diabetic-control), V. rufa extract (diabetic-V. rufa), or glibenclamide (diabetic-GBD). Total antioxidant capacity; levels of thiobarbituric acid reactive substances, reduced glutathione, and sulfhydryls; and superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities were measured in the pancreas. Biochemical analysis of serum total cholesterol and fractions, triglycerides, creatinine, urea, acid uric, ALP, γ-GT, AST, and ALT was performed, and pancreatic β-cells positive for insulin were evaluated by immunohistochemistry. Rats treated with extract exhibited a decrease in fasting blood glucose compared with levels in diabetic control rats. GPx activity and sulfhydryl levels were significantly lower in diabetic-V. rufa rats compared with those of diabetic-control rats. V. rufa extract acted to normalize the biochemical alterations found in diabetic rats (diabetic-controls), as demonstrated by increases in urea, HDL, ALP, AST, and ALT. Reduction in blood glucose was independent of an increase in insulin. The V. rufa extract was found to be composed of free sugars (inositol, galactose, glucose, mannose, sucrose, arabinose, and ribose) as the main metabolites. Thus, aqueous extract of the stem bark of V. rufa is capable of reducing blood glucose, resulting in an antioxidant effect on the pancreatic tissue of STZ-diabetic rats.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28981518</pmid><doi>10.1371/journal.pone.0184807</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-10, Vol.12 (10), p.e0184807-e0184807 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1991851084 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Adenosine triphosphatase Animals Antioxidants Antioxidants - pharmacology Arabinose Bark Biochemical analysis Biology and Life Sciences Blood Blood Glucose Body Weight - drug effects Care and treatment Catalase Catalase - metabolism Chemical properties Cholesterol Creatinine Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - metabolism Enzymes Galactose Glibenclamide Glucose Glutathione Glutathione - metabolism Glutathione peroxidase High density lipoprotein Immunohistochemistry Inositol Insulin Magnoliopsida Male Mannose Medicine and Health Sciences Metabolites Oxidative stress Oxidative Stress - drug effects Pancreas Pancreas - drug effects Pancreas - metabolism Peroxidase Physical Sciences Phytochemicals Phytotherapy Plant Extracts - pharmacology Rats Rats, Wistar Ribose Rodents Sucrose Sugar Superoxide dismutase Superoxide Dismutase - metabolism Thiobarbituric acid Thiobarbituric Acid Reactive Substances - metabolism Triglycerides Urea Vochysiaceae Water treatment |
| title | Phytochemical characterization of the Vochysia rufa (Vochysiaceae) extract and its effects on oxidative stress in the pancreata of streptozotocin-induced diabetic rats |
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