Clonality, virulence determinants, and profiles of resistance of clinical Acinetobacter baumannii isolates obtained from a Spanish hospital
Acinetobacter baumannii is a nosocomial pathogen that is showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this s...
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| description | Acinetobacter baumannii is a nosocomial pathogen that is showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this study, we aim at characterizing A. baumannii isolated from a Spanish hospital in terms of antimicrobial susceptibility, clonality, carbapenemase genes harbored, and virulence determinants expressed.
Fifty nine clinical bloodstream isolates were obtained from 2009 until 2013. Antimicrobial Susceptibility Testing was performed according to the CLSI guidelines. PFGE and tri-locus PCR typing were then performed in order to determine local and international clonality. PCRs for the detection of common carbapenemases were also performed. Production of hemolysis, biofilms, siderophores, surface motility, and proteolysis were determined phenotypically. Doubling times for selected strains were also calculated. Finally, statistical analysis for detecting associations between these factors was conducted.
Carbapenem non-susceptibility was 84.75%, suggesting the immediate need for intervention. PFGE showed the distribution of the majority of the isolates among 7 clusters. Although all three ICs were detected, IC II was predominant at 71.19%. blaOXA-24-like was the most prevalent carbapenemase (62.71%), followed by blaOXA-58-like (13.56%), and blaOXA-23-like (11.86%). Strains pertaining to IC II, and those harboring blaOXA-24-like, were positively associated with α-hemolysis, production of strong biofilms, and siderophore production. Harboring blaOXA-23-like and blaOXA-58-like was associated with attenuated virulence. These associations suggest that an interplay exists between these factors that could be locally exploited.
An alarmingly high rate of carbapenem non-susceptibility has been detected in this study. There was a predominance of IC II and blaOXA-24-like, and those factors were associated with heightened expression of virulence determinants. This association could be exploited for modifying treatment regimens and for improving on infection control protocols in this hospital. |
| doi_str_mv | 10.1371/journal.pone.0176824 |
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Fifty nine clinical bloodstream isolates were obtained from 2009 until 2013. Antimicrobial Susceptibility Testing was performed according to the CLSI guidelines. PFGE and tri-locus PCR typing were then performed in order to determine local and international clonality. PCRs for the detection of common carbapenemases were also performed. Production of hemolysis, biofilms, siderophores, surface motility, and proteolysis were determined phenotypically. Doubling times for selected strains were also calculated. Finally, statistical analysis for detecting associations between these factors was conducted.
Carbapenem non-susceptibility was 84.75%, suggesting the immediate need for intervention. PFGE showed the distribution of the majority of the isolates among 7 clusters. Although all three ICs were detected, IC II was predominant at 71.19%. blaOXA-24-like was the most prevalent carbapenemase (62.71%), followed by blaOXA-58-like (13.56%), and blaOXA-23-like (11.86%). Strains pertaining to IC II, and those harboring blaOXA-24-like, were positively associated with α-hemolysis, production of strong biofilms, and siderophore production. Harboring blaOXA-23-like and blaOXA-58-like was associated with attenuated virulence. These associations suggest that an interplay exists between these factors that could be locally exploited.
An alarmingly high rate of carbapenem non-susceptibility has been detected in this study. There was a predominance of IC II and blaOXA-24-like, and those factors were associated with heightened expression of virulence determinants. This association could be exploited for modifying treatment regimens and for improving on infection control protocols in this hospital.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0176824</identifier><identifier>PMID: 28448572</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acinetobacter baumannii ; Acinetobacter baumannii - drug effects ; Acinetobacter baumannii - genetics ; Acinetobacter baumannii - isolation & purification ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; Bacterial Proteins - genetics ; beta-Lactamases - genetics ; Biofilms ; Biology and Life Sciences ; Carbapenemase ; Carbapenems - pharmacology ; Clone Cells ; Cloning ; Determinants ; Drug resistance ; Drug Resistance, Bacterial - genetics ; Epidemics ; Hemolysis ; Hospitals ; Infections ; Medicine and Health Sciences ; Microbial Sensitivity Tests ; Mortality ; Multidrug resistance ; Nosocomial infection ; Proteolysis ; Research and Analysis Methods ; Siderophores ; Spain ; Statistical analysis ; Strains (organisms) ; Studies ; Typing ; Virulence ; Virulence - genetics</subject><ispartof>PloS one, 2017-04, Vol.12 (4), p.e0176824-e0176824</ispartof><rights>2017 Dahdouh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Dahdouh et al 2017 Dahdouh et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-be84d5e2592689984b7b97acd8813ae8c3a9b05bd4565d55932b2538073c82353</citedby><cites>FETCH-LOGICAL-c526t-be84d5e2592689984b7b97acd8813ae8c3a9b05bd4565d55932b2538073c82353</cites><orcidid>0000-0003-2309-4129 ; 0000-0001-6173-5711</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407824/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407824/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28448572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nübel, Ulrich</contributor><creatorcontrib>Dahdouh, Elias</creatorcontrib><creatorcontrib>Gómez-Gil, Rosa</creatorcontrib><creatorcontrib>Pacho, Sonsoles</creatorcontrib><creatorcontrib>Mingorance, Jesús</creatorcontrib><creatorcontrib>Daoud, Ziad</creatorcontrib><creatorcontrib>Suárez, Monica</creatorcontrib><title>Clonality, virulence determinants, and profiles of resistance of clinical Acinetobacter baumannii isolates obtained from a Spanish hospital</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Acinetobacter baumannii is a nosocomial pathogen that is showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this study, we aim at characterizing A. baumannii isolated from a Spanish hospital in terms of antimicrobial susceptibility, clonality, carbapenemase genes harbored, and virulence determinants expressed.
Fifty nine clinical bloodstream isolates were obtained from 2009 until 2013. Antimicrobial Susceptibility Testing was performed according to the CLSI guidelines. PFGE and tri-locus PCR typing were then performed in order to determine local and international clonality. PCRs for the detection of common carbapenemases were also performed. Production of hemolysis, biofilms, siderophores, surface motility, and proteolysis were determined phenotypically. Doubling times for selected strains were also calculated. Finally, statistical analysis for detecting associations between these factors was conducted.
Carbapenem non-susceptibility was 84.75%, suggesting the immediate need for intervention. PFGE showed the distribution of the majority of the isolates among 7 clusters. Although all three ICs were detected, IC II was predominant at 71.19%. blaOXA-24-like was the most prevalent carbapenemase (62.71%), followed by blaOXA-58-like (13.56%), and blaOXA-23-like (11.86%). Strains pertaining to IC II, and those harboring blaOXA-24-like, were positively associated with α-hemolysis, production of strong biofilms, and siderophore production. Harboring blaOXA-23-like and blaOXA-58-like was associated with attenuated virulence. These associations suggest that an interplay exists between these factors that could be locally exploited.
An alarmingly high rate of carbapenem non-susceptibility has been detected in this study. There was a predominance of IC II and blaOXA-24-like, and those factors were associated with heightened expression of virulence determinants. This association could be exploited for modifying treatment regimens and for improving on infection control protocols in this hospital.</description><subject>Acinetobacter baumannii</subject><subject>Acinetobacter baumannii - drug effects</subject><subject>Acinetobacter baumannii - genetics</subject><subject>Acinetobacter baumannii - isolation & purification</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Bacterial Proteins - genetics</subject><subject>beta-Lactamases - genetics</subject><subject>Biofilms</subject><subject>Biology and Life Sciences</subject><subject>Carbapenemase</subject><subject>Carbapenems - pharmacology</subject><subject>Clone Cells</subject><subject>Cloning</subject><subject>Determinants</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Epidemics</subject><subject>Hemolysis</subject><subject>Hospitals</subject><subject>Infections</subject><subject>Medicine and Health Sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Mortality</subject><subject>Multidrug resistance</subject><subject>Nosocomial infection</subject><subject>Proteolysis</subject><subject>Research and Analysis Methods</subject><subject>Siderophores</subject><subject>Spain</subject><subject>Statistical analysis</subject><subject>Strains (organisms)</subject><subject>Studies</subject><subject>Typing</subject><subject>Virulence</subject><subject>Virulence - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dahdouh, Elias</au><au>Gómez-Gil, Rosa</au><au>Pacho, Sonsoles</au><au>Mingorance, Jesús</au><au>Daoud, Ziad</au><au>Suárez, Monica</au><au>Nübel, Ulrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonality, virulence determinants, and profiles of resistance of clinical Acinetobacter baumannii isolates obtained from a Spanish hospital</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-04-27</date><risdate>2017</risdate><volume>12</volume><issue>4</issue><spage>e0176824</spage><epage>e0176824</epage><pages>e0176824-e0176824</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Acinetobacter baumannii is a nosocomial pathogen that is showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this study, we aim at characterizing A. baumannii isolated from a Spanish hospital in terms of antimicrobial susceptibility, clonality, carbapenemase genes harbored, and virulence determinants expressed.
Fifty nine clinical bloodstream isolates were obtained from 2009 until 2013. Antimicrobial Susceptibility Testing was performed according to the CLSI guidelines. PFGE and tri-locus PCR typing were then performed in order to determine local and international clonality. PCRs for the detection of common carbapenemases were also performed. Production of hemolysis, biofilms, siderophores, surface motility, and proteolysis were determined phenotypically. Doubling times for selected strains were also calculated. Finally, statistical analysis for detecting associations between these factors was conducted.
Carbapenem non-susceptibility was 84.75%, suggesting the immediate need for intervention. PFGE showed the distribution of the majority of the isolates among 7 clusters. Although all three ICs were detected, IC II was predominant at 71.19%. blaOXA-24-like was the most prevalent carbapenemase (62.71%), followed by blaOXA-58-like (13.56%), and blaOXA-23-like (11.86%). Strains pertaining to IC II, and those harboring blaOXA-24-like, were positively associated with α-hemolysis, production of strong biofilms, and siderophore production. Harboring blaOXA-23-like and blaOXA-58-like was associated with attenuated virulence. These associations suggest that an interplay exists between these factors that could be locally exploited.
An alarmingly high rate of carbapenem non-susceptibility has been detected in this study. There was a predominance of IC II and blaOXA-24-like, and those factors were associated with heightened expression of virulence determinants. This association could be exploited for modifying treatment regimens and for improving on infection control protocols in this hospital.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28448572</pmid><doi>10.1371/journal.pone.0176824</doi><orcidid>https://orcid.org/0000-0003-2309-4129</orcidid><orcidid>https://orcid.org/0000-0001-6173-5711</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2017-04, Vol.12 (4), p.e0176824-e0176824 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1990009101 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acinetobacter baumannii Acinetobacter baumannii - drug effects Acinetobacter baumannii - genetics Acinetobacter baumannii - isolation & purification Antibiotics Antiinfectives and antibacterials Antimicrobial agents Bacterial Proteins - genetics beta-Lactamases - genetics Biofilms Biology and Life Sciences Carbapenemase Carbapenems - pharmacology Clone Cells Cloning Determinants Drug resistance Drug Resistance, Bacterial - genetics Epidemics Hemolysis Hospitals Infections Medicine and Health Sciences Microbial Sensitivity Tests Mortality Multidrug resistance Nosocomial infection Proteolysis Research and Analysis Methods Siderophores Spain Statistical analysis Strains (organisms) Studies Typing Virulence Virulence - genetics |
| title | Clonality, virulence determinants, and profiles of resistance of clinical Acinetobacter baumannii isolates obtained from a Spanish hospital |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A50%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clonality,%20virulence%20determinants,%20and%20profiles%20of%20resistance%20of%20clinical%20Acinetobacter%20baumannii%20isolates%20obtained%20from%20a%20Spanish%20hospital&rft.jtitle=PloS%20one&rft.au=Dahdouh,%20Elias&rft.date=2017-04-27&rft.volume=12&rft.issue=4&rft.spage=e0176824&rft.epage=e0176824&rft.pages=e0176824-e0176824&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0176824&rft_dat=%3Cproquest_plos_%3E1990009101%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1990009101&rft_id=info:pmid/28448572&rft_doaj_id=oai_doaj_org_article_225a9a02dff942159c271f10d6656363&rfr_iscdi=true |