Dual function of EDTA with silver nanoparticles for root canal treatment-A novel modification
The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron m...
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| creator | Martinez-Andrade, Juan M Avalos-Borja, Miguel Vilchis-Nestor, Alfredo R Sanchez-Vargas, Luis O Castro-Longoria, Ernestina |
| description | The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 μg/ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT. |
| doi_str_mv | 10.1371/journal.pone.0190866 |
| format | Article |
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The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 μg/ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0190866</identifier><identifier>PMID: 29346398</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Absorption spectroscopy ; Acids ; Antiinfectives and antibacterials ; Antimicrobial agents ; Atomic absorption analysis ; Atomic absorption spectroscopy ; Atomic beam spectroscopy ; Atomic force microscopy ; Bacteria ; Biofilms ; Biology and Life Sciences ; Candida albicans ; Chelation ; Cultures ; Demineralizing ; Dentin ; Edetic acid ; EDTA ; Electron microscopy ; Endodontics ; Ethylenediaminetetraacetic acids ; Health aspects ; Ligands ; Medicine and Health Sciences ; Methods ; Microhardness ; Microorganisms ; Nanoparticles ; Physical Sciences ; Production capacity ; Research and Analysis Methods ; Root canal therapy ; Root canals ; Scanning electron microscopy ; Silver ; Silver compounds ; Smear ; Spectral analysis ; Spectroscopy ; Staphylococcus aureus ; Transmission electron microscopy</subject><ispartof>PloS one, 2018-01, Vol.13 (1), p.e0190866-e0190866</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Martinez-Andrade et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Martinez-Andrade et al 2018 Martinez-Andrade et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-288795c082b20b8cbabd69796de881cfe32d5d52e1f6cf96fd4c0c8c5354ec33</citedby><cites>FETCH-LOGICAL-c692t-288795c082b20b8cbabd69796de881cfe32d5d52e1f6cf96fd4c0c8c5354ec33</cites><orcidid>0000-0002-7464-8964</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773103/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773103/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29346398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mukherjee, Amitava</contributor><creatorcontrib>Martinez-Andrade, Juan M</creatorcontrib><creatorcontrib>Avalos-Borja, Miguel</creatorcontrib><creatorcontrib>Vilchis-Nestor, Alfredo R</creatorcontrib><creatorcontrib>Sanchez-Vargas, Luis O</creatorcontrib><creatorcontrib>Castro-Longoria, Ernestina</creatorcontrib><title>Dual function of EDTA with silver nanoparticles for root canal treatment-A novel modification</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 μg/ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT.</description><subject>Absorption spectroscopy</subject><subject>Acids</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Atomic absorption analysis</subject><subject>Atomic absorption spectroscopy</subject><subject>Atomic beam spectroscopy</subject><subject>Atomic force microscopy</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Biology and Life Sciences</subject><subject>Candida albicans</subject><subject>Chelation</subject><subject>Cultures</subject><subject>Demineralizing</subject><subject>Dentin</subject><subject>Edetic acid</subject><subject>EDTA</subject><subject>Electron microscopy</subject><subject>Endodontics</subject><subject>Ethylenediaminetetraacetic acids</subject><subject>Health 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function of EDTA with silver nanoparticles for root canal treatment-A novel modification</title><author>Martinez-Andrade, Juan M ; Avalos-Borja, Miguel ; Vilchis-Nestor, Alfredo R ; Sanchez-Vargas, Luis O ; Castro-Longoria, Ernestina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-288795c082b20b8cbabd69796de881cfe32d5d52e1f6cf96fd4c0c8c5354ec33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Absorption spectroscopy</topic><topic>Acids</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Atomic absorption analysis</topic><topic>Atomic absorption spectroscopy</topic><topic>Atomic beam spectroscopy</topic><topic>Atomic force microscopy</topic><topic>Bacteria</topic><topic>Biofilms</topic><topic>Biology and Life Sciences</topic><topic>Candida albicans</topic><topic>Chelation</topic><topic>Cultures</topic><topic>Demineralizing</topic><topic>Dentin</topic><topic>Edetic acid</topic><topic>EDTA</topic><topic>Electron microscopy</topic><topic>Endodontics</topic><topic>Ethylenediaminetetraacetic acids</topic><topic>Health aspects</topic><topic>Ligands</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Microhardness</topic><topic>Microorganisms</topic><topic>Nanoparticles</topic><topic>Physical Sciences</topic><topic>Production capacity</topic><topic>Research and Analysis Methods</topic><topic>Root canal therapy</topic><topic>Root canals</topic><topic>Scanning electron microscopy</topic><topic>Silver</topic><topic>Silver compounds</topic><topic>Smear</topic><topic>Spectral analysis</topic><topic>Spectroscopy</topic><topic>Staphylococcus aureus</topic><topic>Transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez-Andrade, 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Amitava</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual function of EDTA with silver nanoparticles for root canal treatment-A novel modification</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-01-18</date><risdate>2018</risdate><volume>13</volume><issue>1</issue><spage>e0190866</spage><epage>e0190866</epage><pages>e0190866-e0190866</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 μg/ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29346398</pmid><doi>10.1371/journal.pone.0190866</doi><tpages>e0190866</tpages><orcidid>https://orcid.org/0000-0002-7464-8964</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-01, Vol.13 (1), p.e0190866-e0190866 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1989015048 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Absorption spectroscopy Acids Antiinfectives and antibacterials Antimicrobial agents Atomic absorption analysis Atomic absorption spectroscopy Atomic beam spectroscopy Atomic force microscopy Bacteria Biofilms Biology and Life Sciences Candida albicans Chelation Cultures Demineralizing Dentin Edetic acid EDTA Electron microscopy Endodontics Ethylenediaminetetraacetic acids Health aspects Ligands Medicine and Health Sciences Methods Microhardness Microorganisms Nanoparticles Physical Sciences Production capacity Research and Analysis Methods Root canal therapy Root canals Scanning electron microscopy Silver Silver compounds Smear Spectral analysis Spectroscopy Staphylococcus aureus Transmission electron microscopy |
| title | Dual function of EDTA with silver nanoparticles for root canal treatment-A novel modification |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T03%3A17%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dual%20function%20of%20EDTA%20with%20silver%20nanoparticles%20for%20root%20canal%20treatment-A%20novel%20modification&rft.jtitle=PloS%20one&rft.au=Martinez-Andrade,%20Juan%20M&rft.date=2018-01-18&rft.volume=13&rft.issue=1&rft.spage=e0190866&rft.epage=e0190866&rft.pages=e0190866-e0190866&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0190866&rft_dat=%3Cgale_plos_%3EA523860338%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1989015048&rft_id=info:pmid/29346398&rft_galeid=A523860338&rft_doaj_id=oai_doaj_org_article_8c2d1382af4d46e2be4818d98dcef4a4&rfr_iscdi=true |