Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin
Samia cynthia ricini (Lepidoptera: Saturniidae) is an important commercial silk-producing insect; however, in contrast to the silkworm, mulberry leaves are toxic to this insect because the leaves contain the component 1-deoxynojirimycin (DNJ). A transcriptomic analysis of eri-silkworm haemolymph was...
Gespeichert in:
| Veröffentlicht in: | PloS one 2018-01, Vol.13 (1), p.e0191080 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | e0191080 |
| container_title | PloS one |
| container_volume | 13 |
| creator | Zhang, Shang-Zhi Yu, Hai-Zhong Deng, Ming-Jie Ma, Yan Fei, Dong-Qiong Wang, Jie Li, Zhen Meng, Yan Xu, Jia-Ping |
| description | Samia cynthia ricini (Lepidoptera: Saturniidae) is an important commercial silk-producing insect; however, in contrast to the silkworm, mulberry leaves are toxic to this insect because the leaves contain the component 1-deoxynojirimycin (DNJ). A transcriptomic analysis of eri-silkworm haemolymph was conducted to examine the genes related to different metabolic pathways and to elucidate the molecular mechanism underlying eri-silkworm haemolymph responses to DNJ. Eight hundred sixty-five differentially expressed genes (DEGs) were identified, among which 577 DEGs were up-regulated and 288 DEGs were down-regulated in the 2% DNJ group compared to control (ddH2O) after 12h. Based on the results of the functional analysis, these DEGs were associated with ribosomes, glycolysis, N-glycan biosynthesis, and oxidative phosphorylation. In particular, according to the KEGG analysis, 138 DEGs were involved in energy metabolism, glycometabolism and lipid metabolism, and the changes in the expression of nine DEGs were confirmed by reverse transcription quantitative PCR (RT-qPCR). Thus, DNJ induced significant metabolic alterations in eri-silkworm haemolymph. These results will lay the foundation for research into the toxic effects of DNJ on eri-silkworm as a model and provide a reference for the exploitation of new drugs in humans. |
| doi_str_mv | 10.1371/journal.pone.0191080 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1986672045</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A522513271</galeid><doaj_id>oai_doaj_org_article_7f7a170edab74230aec64c2f43dc772e</doaj_id><sourcerecordid>A522513271</sourcerecordid><originalsourceid>FETCH-LOGICAL-c593t-250d2c773d09542730caad9f64e560d59c0ef46c360f7eb0b3e603a0891f84593</originalsourceid><addsrcrecordid>eNp1UsuO1DAQjBCIXRb-AIElLnDI4Ecek8tKqxGPlVbiAJytjtOZ6SGxg50ZyN_wqXh2sqsdCeSDrXZVdbldSfJS8IVQpXi_dTtvoVsMzuKCi0rwJX-UnItKybSQXD1-cD5LnoWw5TxXy6J4mpzJeJEtK3We_Fm5fgAPI-2RjR5sMJ6G0fXIIKpPgQLzuEfoAgu0ttSSATuyHkeoXUeGQTfige9sYGQZekoDdT9-Od-zt1-hJ2BmsuMm7p4MWXrHNoC966Z-2BwYHkN8Q4jtHRNpg-73ZN2WPPVThD9PnrSxOb6Y94vk-8cP31af05svn65XVzepySs1pjLnjTRlqRpe5ZksFTcATdUWGeYFb_LKcGyzwqiCtyXWvFZYcAV8WYl2mUWJi-T1UXfoXNDzcIMWVZxYKXmWR8T1EdE42Ooh-gM_aQekbwvOrzX4kUyHumxLECXHBuoyk4oDmiIzss1UEz1KjFqXc7dd3WNj0MbZdyeipzeWNnrt9joviyz-fhR4Mwt493OHYfyP5Rm1huiKbOuimOkpGH2VS5kLJW-1Fv9AxdVgTybGq6VYPyFkR4LxLgSP7b1xwfUhnHdm9CGceg5npL16-Oh70l0a1V9pC-Y5</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1986672045</pqid></control><display><type>article</type><title>Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin</title><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Zhang, Shang-Zhi ; Yu, Hai-Zhong ; Deng, Ming-Jie ; Ma, Yan ; Fei, Dong-Qiong ; Wang, Jie ; Li, Zhen ; Meng, Yan ; Xu, Jia-Ping</creator><contributor>Ling, Erjun</contributor><creatorcontrib>Zhang, Shang-Zhi ; Yu, Hai-Zhong ; Deng, Ming-Jie ; Ma, Yan ; Fei, Dong-Qiong ; Wang, Jie ; Li, Zhen ; Meng, Yan ; Xu, Jia-Ping ; Ling, Erjun</creatorcontrib><description>Samia cynthia ricini (Lepidoptera: Saturniidae) is an important commercial silk-producing insect; however, in contrast to the silkworm, mulberry leaves are toxic to this insect because the leaves contain the component 1-deoxynojirimycin (DNJ). A transcriptomic analysis of eri-silkworm haemolymph was conducted to examine the genes related to different metabolic pathways and to elucidate the molecular mechanism underlying eri-silkworm haemolymph responses to DNJ. Eight hundred sixty-five differentially expressed genes (DEGs) were identified, among which 577 DEGs were up-regulated and 288 DEGs were down-regulated in the 2% DNJ group compared to control (ddH2O) after 12h. Based on the results of the functional analysis, these DEGs were associated with ribosomes, glycolysis, N-glycan biosynthesis, and oxidative phosphorylation. In particular, according to the KEGG analysis, 138 DEGs were involved in energy metabolism, glycometabolism and lipid metabolism, and the changes in the expression of nine DEGs were confirmed by reverse transcription quantitative PCR (RT-qPCR). Thus, DNJ induced significant metabolic alterations in eri-silkworm haemolymph. These results will lay the foundation for research into the toxic effects of DNJ on eri-silkworm as a model and provide a reference for the exploitation of new drugs in humans.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0191080</identifier><identifier>PMID: 29324893</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology and Life Sciences ; Biosynthesis ; Diabetes ; Drugs ; Energy ; Energy metabolism ; Exploitation ; Food ; Functional analysis ; Gene expression ; Genes ; Genetic aspects ; Glycan ; Glycolysis ; Glycoproteins ; Hemolymph ; Insects ; Insulin ; Kinases ; Leaves ; Lepidoptera ; Life sciences ; Lipid metabolism ; Lipids ; Medicine and Health Sciences ; Metabolic pathways ; Metabolism ; Oxidative phosphorylation ; Phosphorylation ; Physiological aspects ; Reverse transcription ; Ribosomes ; Samia cynthia ricini ; Saturniidae ; Silk ; Toxicity ; Transcription (Genetics)</subject><ispartof>PloS one, 2018-01, Vol.13 (1), p.e0191080</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Zhang et al 2018 Zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-250d2c773d09542730caad9f64e560d59c0ef46c360f7eb0b3e603a0891f84593</citedby><cites>FETCH-LOGICAL-c593t-250d2c773d09542730caad9f64e560d59c0ef46c360f7eb0b3e603a0891f84593</cites><orcidid>0000-0001-9020-7248</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764371/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764371/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29324893$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ling, Erjun</contributor><creatorcontrib>Zhang, Shang-Zhi</creatorcontrib><creatorcontrib>Yu, Hai-Zhong</creatorcontrib><creatorcontrib>Deng, Ming-Jie</creatorcontrib><creatorcontrib>Ma, Yan</creatorcontrib><creatorcontrib>Fei, Dong-Qiong</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Li, Zhen</creatorcontrib><creatorcontrib>Meng, Yan</creatorcontrib><creatorcontrib>Xu, Jia-Ping</creatorcontrib><title>Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Samia cynthia ricini (Lepidoptera: Saturniidae) is an important commercial silk-producing insect; however, in contrast to the silkworm, mulberry leaves are toxic to this insect because the leaves contain the component 1-deoxynojirimycin (DNJ). A transcriptomic analysis of eri-silkworm haemolymph was conducted to examine the genes related to different metabolic pathways and to elucidate the molecular mechanism underlying eri-silkworm haemolymph responses to DNJ. Eight hundred sixty-five differentially expressed genes (DEGs) were identified, among which 577 DEGs were up-regulated and 288 DEGs were down-regulated in the 2% DNJ group compared to control (ddH2O) after 12h. Based on the results of the functional analysis, these DEGs were associated with ribosomes, glycolysis, N-glycan biosynthesis, and oxidative phosphorylation. In particular, according to the KEGG analysis, 138 DEGs were involved in energy metabolism, glycometabolism and lipid metabolism, and the changes in the expression of nine DEGs were confirmed by reverse transcription quantitative PCR (RT-qPCR). Thus, DNJ induced significant metabolic alterations in eri-silkworm haemolymph. These results will lay the foundation for research into the toxic effects of DNJ on eri-silkworm as a model and provide a reference for the exploitation of new drugs in humans.</description><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Diabetes</subject><subject>Drugs</subject><subject>Energy</subject><subject>Energy metabolism</subject><subject>Exploitation</subject><subject>Food</subject><subject>Functional analysis</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Glycan</subject><subject>Glycolysis</subject><subject>Glycoproteins</subject><subject>Hemolymph</subject><subject>Insects</subject><subject>Insulin</subject><subject>Kinases</subject><subject>Leaves</subject><subject>Lepidoptera</subject><subject>Life sciences</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Medicine and Health Sciences</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Oxidative phosphorylation</subject><subject>Phosphorylation</subject><subject>Physiological aspects</subject><subject>Reverse transcription</subject><subject>Ribosomes</subject><subject>Samia cynthia ricini</subject><subject>Saturniidae</subject><subject>Silk</subject><subject>Toxicity</subject><subject>Transcription (Genetics)</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp1UsuO1DAQjBCIXRb-AIElLnDI4Ecek8tKqxGPlVbiAJytjtOZ6SGxg50ZyN_wqXh2sqsdCeSDrXZVdbldSfJS8IVQpXi_dTtvoVsMzuKCi0rwJX-UnItKybSQXD1-cD5LnoWw5TxXy6J4mpzJeJEtK3We_Fm5fgAPI-2RjR5sMJ6G0fXIIKpPgQLzuEfoAgu0ttSSATuyHkeoXUeGQTfige9sYGQZekoDdT9-Od-zt1-hJ2BmsuMm7p4MWXrHNoC966Z-2BwYHkN8Q4jtHRNpg-73ZN2WPPVThD9PnrSxOb6Y94vk-8cP31af05svn65XVzepySs1pjLnjTRlqRpe5ZksFTcATdUWGeYFb_LKcGyzwqiCtyXWvFZYcAV8WYl2mUWJi-T1UXfoXNDzcIMWVZxYKXmWR8T1EdE42Ooh-gM_aQekbwvOrzX4kUyHumxLECXHBuoyk4oDmiIzss1UEz1KjFqXc7dd3WNj0MbZdyeipzeWNnrt9joviyz-fhR4Mwt493OHYfyP5Rm1huiKbOuimOkpGH2VS5kLJW-1Fv9AxdVgTybGq6VYPyFkR4LxLgSP7b1xwfUhnHdm9CGceg5npL16-Oh70l0a1V9pC-Y5</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Zhang, Shang-Zhi</creator><creator>Yu, Hai-Zhong</creator><creator>Deng, Ming-Jie</creator><creator>Ma, Yan</creator><creator>Fei, Dong-Qiong</creator><creator>Wang, Jie</creator><creator>Li, Zhen</creator><creator>Meng, Yan</creator><creator>Xu, Jia-Ping</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-9020-7248</orcidid></search><sort><creationdate>20180101</creationdate><title>Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin</title><author>Zhang, Shang-Zhi ; Yu, Hai-Zhong ; Deng, Ming-Jie ; Ma, Yan ; Fei, Dong-Qiong ; Wang, Jie ; Li, Zhen ; Meng, Yan ; Xu, Jia-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-250d2c773d09542730caad9f64e560d59c0ef46c360f7eb0b3e603a0891f84593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Diabetes</topic><topic>Drugs</topic><topic>Energy</topic><topic>Energy metabolism</topic><topic>Exploitation</topic><topic>Food</topic><topic>Functional analysis</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Glycan</topic><topic>Glycolysis</topic><topic>Glycoproteins</topic><topic>Hemolymph</topic><topic>Insects</topic><topic>Insulin</topic><topic>Kinases</topic><topic>Leaves</topic><topic>Lepidoptera</topic><topic>Life sciences</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Medicine and Health Sciences</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>Oxidative phosphorylation</topic><topic>Phosphorylation</topic><topic>Physiological aspects</topic><topic>Reverse transcription</topic><topic>Ribosomes</topic><topic>Samia cynthia ricini</topic><topic>Saturniidae</topic><topic>Silk</topic><topic>Toxicity</topic><topic>Transcription (Genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Shang-Zhi</creatorcontrib><creatorcontrib>Yu, Hai-Zhong</creatorcontrib><creatorcontrib>Deng, Ming-Jie</creatorcontrib><creatorcontrib>Ma, Yan</creatorcontrib><creatorcontrib>Fei, Dong-Qiong</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Li, Zhen</creatorcontrib><creatorcontrib>Meng, Yan</creatorcontrib><creatorcontrib>Xu, Jia-Ping</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Shang-Zhi</au><au>Yu, Hai-Zhong</au><au>Deng, Ming-Jie</au><au>Ma, Yan</au><au>Fei, Dong-Qiong</au><au>Wang, Jie</au><au>Li, Zhen</au><au>Meng, Yan</au><au>Xu, Jia-Ping</au><au>Ling, Erjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>13</volume><issue>1</issue><spage>e0191080</spage><pages>e0191080-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Samia cynthia ricini (Lepidoptera: Saturniidae) is an important commercial silk-producing insect; however, in contrast to the silkworm, mulberry leaves are toxic to this insect because the leaves contain the component 1-deoxynojirimycin (DNJ). A transcriptomic analysis of eri-silkworm haemolymph was conducted to examine the genes related to different metabolic pathways and to elucidate the molecular mechanism underlying eri-silkworm haemolymph responses to DNJ. Eight hundred sixty-five differentially expressed genes (DEGs) were identified, among which 577 DEGs were up-regulated and 288 DEGs were down-regulated in the 2% DNJ group compared to control (ddH2O) after 12h. Based on the results of the functional analysis, these DEGs were associated with ribosomes, glycolysis, N-glycan biosynthesis, and oxidative phosphorylation. In particular, according to the KEGG analysis, 138 DEGs were involved in energy metabolism, glycometabolism and lipid metabolism, and the changes in the expression of nine DEGs were confirmed by reverse transcription quantitative PCR (RT-qPCR). Thus, DNJ induced significant metabolic alterations in eri-silkworm haemolymph. These results will lay the foundation for research into the toxic effects of DNJ on eri-silkworm as a model and provide a reference for the exploitation of new drugs in humans.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29324893</pmid><doi>10.1371/journal.pone.0191080</doi><orcidid>https://orcid.org/0000-0001-9020-7248</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-01, Vol.13 (1), p.e0191080 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1986672045 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biology and Life Sciences Biosynthesis Diabetes Drugs Energy Energy metabolism Exploitation Food Functional analysis Gene expression Genes Genetic aspects Glycan Glycolysis Glycoproteins Hemolymph Insects Insulin Kinases Leaves Lepidoptera Life sciences Lipid metabolism Lipids Medicine and Health Sciences Metabolic pathways Metabolism Oxidative phosphorylation Phosphorylation Physiological aspects Reverse transcription Ribosomes Samia cynthia ricini Saturniidae Silk Toxicity Transcription (Genetics) |
| title | Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T08%3A48%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20transcriptome%20analysis%20reveals%20significant%20metabolic%20alterations%20in%20eri-silkworm%20(Samia%20cynthia%20ricini)%20haemolymph%20in%20response%20to%201-deoxynojirimycin&rft.jtitle=PloS%20one&rft.au=Zhang,%20Shang-Zhi&rft.date=2018-01-01&rft.volume=13&rft.issue=1&rft.spage=e0191080&rft.pages=e0191080-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0191080&rft_dat=%3Cgale_plos_%3EA522513271%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1986672045&rft_id=info:pmid/29324893&rft_galeid=A522513271&rft_doaj_id=oai_doaj_org_article_7f7a170edab74230aec64c2f43dc772e&rfr_iscdi=true |