Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing

Antibody specificity and diversity are generated through the enzymatic splicing of genomic gene segments within each B cell. Antibodies are heterodimers of heavy- and light-chains encoded on separate loci. We studied the antibody repertoire from pooled, splenic tissue of unimmunized, adult female C5...

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Veröffentlicht in:	PloS one 2018-01, Vol.13 (1), p.e0190982-e0190982
Hauptverfasser:	Rettig, Trisha A, Ward, Claire, Bye, Bailey A, Pecaut, Michael J, Chapes, Stephen K
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Animals Antibodies Antibody Diversity Antibody Specificity Antigens B cells B-Lymphocytes - immunology B-Lymphocytes - metabolism Binding sites Biology Biology and Life Sciences Chains Chromosomes Combinatorial analysis Complementarity-determining region 3 Enzymes Experiments Female Gene sequencing High-Throughput Nucleotide Sequencing - methods Immunoglobulin Heavy Chains - genetics Immunoglobulin Light Chains - genetics Immunoglobulin Variable Region - genetics Immunoglobulins Laboratory animals Light Lymphocytes B Medicine and Health Sciences Mice Mice, Inbred C57BL Next-generation sequencing Pools Research and Analysis Methods Rodents Science & Technology - Other Topics Segments Sequence Analysis, DNA - methods Spleen Splicing
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