TC1(C8orf4) regulates hematopoietic stem/progenitor cells and hematopoiesis
Hematopoiesis is a complex process requiring multiple regulators for hematopoietic stem/progenitor cells (HSPC) and differentiation to multi-lineage blood cells. TC1(C8orf4) is implicated in cancers, hematological malignancies and inflammatory activation. Here, we report that Tc1 regulates hematopoi...
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| Veröffentlicht in: | PloS one 2014-06, Vol.9 (6), p.e100311-e100311 |
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| creator | Jung, Yusun Kim, Minsung Soh, Hyunsu Lee, Soyoung Kim, Jungtae Park, Surim Song, Kyuyoung Lee, Inchul |
| description | Hematopoiesis is a complex process requiring multiple regulators for hematopoietic stem/progenitor cells (HSPC) and differentiation to multi-lineage blood cells. TC1(C8orf4) is implicated in cancers, hematological malignancies and inflammatory activation. Here, we report that Tc1 regulates hematopoiesis in mice. Myeloid and lymphoid cells are increased markedly in peripheral blood of Tc1-deleted mice compared to wild type controls. Red blood cells are small-sized but increased in number. The bone marrow of Tc1-/- mice is normocellular histologically. However, Lin-Sca-1+c-Kit+ (LSK) cells are expanded in Tc1-/- mice compared to wild type controls. The expanded population mostly consists of CD150-CD48+ cells, suggesting the expansion of lineage-restricted hematopoietic progenitor cells. Colony forming units (CFU) are increased in Tc1-/- mice bone marrow cells compared to controls. In wild type mice bone marrow, Tc1 is expressed in a limited population of HSPC but not in differentiated cells. Major myeloid transcriptional regulators such as Pu.1 and Cebpα are not up-regulated in Tc1-/- mice bone marrow. Our findings indicate that TC1 is a novel hematopoietic regulator. The mechanisms of TC1-dependent HSPC regulation and lineage determination are unknown. |
| doi_str_mv | 10.1371/journal.pone.0100311 |
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TC1(C8orf4) is implicated in cancers, hematological malignancies and inflammatory activation. Here, we report that Tc1 regulates hematopoiesis in mice. Myeloid and lymphoid cells are increased markedly in peripheral blood of Tc1-deleted mice compared to wild type controls. Red blood cells are small-sized but increased in number. The bone marrow of Tc1-/- mice is normocellular histologically. However, Lin-Sca-1+c-Kit+ (LSK) cells are expanded in Tc1-/- mice compared to wild type controls. The expanded population mostly consists of CD150-CD48+ cells, suggesting the expansion of lineage-restricted hematopoietic progenitor cells. Colony forming units (CFU) are increased in Tc1-/- mice bone marrow cells compared to controls. In wild type mice bone marrow, Tc1 is expressed in a limited population of HSPC but not in differentiated cells. Major myeloid transcriptional regulators such as Pu.1 and Cebpα are not up-regulated in Tc1-/- mice bone marrow. Our findings indicate that TC1 is a novel hematopoietic regulator. The mechanisms of TC1-dependent HSPC regulation and lineage determination are unknown.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0100311</identifier><identifier>PMID: 24937306</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biology and Life Sciences ; Blood ; Blood cells ; Blotting, Western ; Bone marrow ; Bone Marrow - metabolism ; Bone Marrow - pathology ; c-Kit protein ; CD150 antigen ; Cell Differentiation ; Cell Lineage ; Cells (biology) ; Cells, Cultured ; Consortia ; Cytokines ; Erythrocytes ; Female ; Flow Cytometry ; Genomes ; Hematology ; Hematopoiesis ; Hematopoiesis - physiology ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - physiology ; HL-60 Cells ; Humans ; Immunoenzyme Techniques ; Inflammation ; Life sciences ; Lymphoid cells ; Medicine ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microscopy, Fluorescence ; Neoplasm Proteins - physiology ; Parkinson's disease ; Peripheral blood ; Progenitor cells ; PU.1 protein ; Real-Time Polymerase Chain Reaction ; Regulators ; Research and Analysis Methods ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Stem cells ; Transcription</subject><ispartof>PloS one, 2014-06, Vol.9 (6), p.e100311-e100311</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Jung et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Jung et al 2014 Jung et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-bf600ac94e5c574f443a879cd56d848de26ea0ae515e560713f33eb1053cfa763</citedby><cites>FETCH-LOGICAL-c593t-bf600ac94e5c574f443a879cd56d848de26ea0ae515e560713f33eb1053cfa763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061086/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061086/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24937306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Bunting, Kevin D.</contributor><creatorcontrib>Jung, Yusun</creatorcontrib><creatorcontrib>Kim, Minsung</creatorcontrib><creatorcontrib>Soh, Hyunsu</creatorcontrib><creatorcontrib>Lee, Soyoung</creatorcontrib><creatorcontrib>Kim, Jungtae</creatorcontrib><creatorcontrib>Park, Surim</creatorcontrib><creatorcontrib>Song, Kyuyoung</creatorcontrib><creatorcontrib>Lee, Inchul</creatorcontrib><title>TC1(C8orf4) regulates hematopoietic stem/progenitor cells and hematopoiesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hematopoiesis is a complex process requiring multiple regulators for hematopoietic stem/progenitor cells (HSPC) and differentiation to multi-lineage blood cells. TC1(C8orf4) is implicated in cancers, hematological malignancies and inflammatory activation. Here, we report that Tc1 regulates hematopoiesis in mice. Myeloid and lymphoid cells are increased markedly in peripheral blood of Tc1-deleted mice compared to wild type controls. Red blood cells are small-sized but increased in number. The bone marrow of Tc1-/- mice is normocellular histologically. However, Lin-Sca-1+c-Kit+ (LSK) cells are expanded in Tc1-/- mice compared to wild type controls. The expanded population mostly consists of CD150-CD48+ cells, suggesting the expansion of lineage-restricted hematopoietic progenitor cells. Colony forming units (CFU) are increased in Tc1-/- mice bone marrow cells compared to controls. In wild type mice bone marrow, Tc1 is expressed in a limited population of HSPC but not in differentiated cells. Major myeloid transcriptional regulators such as Pu.1 and Cebpα are not up-regulated in Tc1-/- mice bone marrow. Our findings indicate that TC1 is a novel hematopoietic regulator. The mechanisms of TC1-dependent HSPC regulation and lineage determination are unknown.</description><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood cells</subject><subject>Blotting, Western</subject><subject>Bone marrow</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow - pathology</subject><subject>c-Kit protein</subject><subject>CD150 antigen</subject><subject>Cell Differentiation</subject><subject>Cell Lineage</subject><subject>Cells (biology)</subject><subject>Cells, Cultured</subject><subject>Consortia</subject><subject>Cytokines</subject><subject>Erythrocytes</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Genomes</subject><subject>Hematology</subject><subject>Hematopoiesis</subject><subject>Hematopoiesis - physiology</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Inflammation</subject><subject>Life sciences</subject><subject>Lymphoid cells</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Microscopy, Fluorescence</subject><subject>Neoplasm Proteins - physiology</subject><subject>Parkinson's disease</subject><subject>Peripheral blood</subject><subject>Progenitor cells</subject><subject>PU.1 protein</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Regulators</subject><subject>Research and Analysis Methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Stem cells</subject><subject>Transcription</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1r3DAQNaWhSdP-g9IackkPu5GsD8uXQlj6ERroJT2LWXnkaLEtV7IL-feVu07YDUEHidGbN-8NL8s-ULKmrKRXOz-FHtr14HtcE0oIo_RVdkYrVqxkQdjrg_dp9jbGHSGCKSnfZKcFr1jJiDzLft5t6OVG-WD55zxgM7UwYszvsYPRD97h6EweR-yuhuAb7N3oQ26wbWMOfX2Aiy6-y04stBHfL_d59vvb17vNj9Xtr-83m-vblREVG1dbKwkBU3EURpTccs5AlZWphawVVzUWEoEACipQSFJSZhnDLU3qjYVSsvPs0553aH3Uyx6ippXiFU2MKiFu9ojaw04PwXUQHrQHp_8XfGg0hOSsRW2YYgVURDJjuCJWKQpU8G1SwgpezFxflmnTtsPaYD8GaI9Ij396d68b_1dzIilRs9zLhSD4PxPGUXcuziuEHv2UdAtWpsUUpUjQi2fQl90tqAaSAddbn-aamVRfc6p4wUs5o9YvoNKpsXMmhca6VD9q4PsGE3yMAe2TR0r0HLlHMXqOnF4il9o-Hu7nqekxY-wfe3jRsg</recordid><startdate>20140617</startdate><enddate>20140617</enddate><creator>Jung, Yusun</creator><creator>Kim, Minsung</creator><creator>Soh, Hyunsu</creator><creator>Lee, Soyoung</creator><creator>Kim, Jungtae</creator><creator>Park, Surim</creator><creator>Song, Kyuyoung</creator><creator>Lee, Inchul</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140617</creationdate><title>TC1(C8orf4) regulates hematopoietic stem/progenitor cells and hematopoiesis</title><author>Jung, Yusun ; Kim, Minsung ; Soh, Hyunsu ; Lee, Soyoung ; Kim, Jungtae ; Park, Surim ; Song, Kyuyoung ; Lee, Inchul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-bf600ac94e5c574f443a879cd56d848de26ea0ae515e560713f33eb1053cfa763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood cells</topic><topic>Blotting, Western</topic><topic>Bone marrow</topic><topic>Bone Marrow - metabolism</topic><topic>Bone Marrow - pathology</topic><topic>c-Kit protein</topic><topic>CD150 antigen</topic><topic>Cell Differentiation</topic><topic>Cell Lineage</topic><topic>Cells (biology)</topic><topic>Cells, Cultured</topic><topic>Consortia</topic><topic>Cytokines</topic><topic>Erythrocytes</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Genomes</topic><topic>Hematology</topic><topic>Hematopoiesis</topic><topic>Hematopoiesis - physiology</topic><topic>Hematopoietic stem cells</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - physiology</topic><topic>HL-60 Cells</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Inflammation</topic><topic>Life sciences</topic><topic>Lymphoid cells</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Microscopy, Fluorescence</topic><topic>Neoplasm Proteins - physiology</topic><topic>Parkinson's disease</topic><topic>Peripheral blood</topic><topic>Progenitor cells</topic><topic>PU.1 protein</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Regulators</topic><topic>Research and Analysis Methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, Yusun</au><au>Kim, Minsung</au><au>Soh, Hyunsu</au><au>Lee, Soyoung</au><au>Kim, Jungtae</au><au>Park, Surim</au><au>Song, Kyuyoung</au><au>Lee, Inchul</au><au>Bunting, Kevin D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TC1(C8orf4) regulates hematopoietic stem/progenitor cells and hematopoiesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-06-17</date><risdate>2014</risdate><volume>9</volume><issue>6</issue><spage>e100311</spage><epage>e100311</epage><pages>e100311-e100311</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hematopoiesis is a complex process requiring multiple regulators for hematopoietic stem/progenitor cells (HSPC) and differentiation to multi-lineage blood cells. TC1(C8orf4) is implicated in cancers, hematological malignancies and inflammatory activation. Here, we report that Tc1 regulates hematopoiesis in mice. Myeloid and lymphoid cells are increased markedly in peripheral blood of Tc1-deleted mice compared to wild type controls. Red blood cells are small-sized but increased in number. The bone marrow of Tc1-/- mice is normocellular histologically. However, Lin-Sca-1+c-Kit+ (LSK) cells are expanded in Tc1-/- mice compared to wild type controls. The expanded population mostly consists of CD150-CD48+ cells, suggesting the expansion of lineage-restricted hematopoietic progenitor cells. Colony forming units (CFU) are increased in Tc1-/- mice bone marrow cells compared to controls. In wild type mice bone marrow, Tc1 is expressed in a limited population of HSPC but not in differentiated cells. Major myeloid transcriptional regulators such as Pu.1 and Cebpα are not up-regulated in Tc1-/- mice bone marrow. Our findings indicate that TC1 is a novel hematopoietic regulator. The mechanisms of TC1-dependent HSPC regulation and lineage determination are unknown.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24937306</pmid><doi>10.1371/journal.pone.0100311</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biology and Life Sciences Blood Blood cells Blotting, Western Bone marrow Bone Marrow - metabolism Bone Marrow - pathology c-Kit protein CD150 antigen Cell Differentiation Cell Lineage Cells (biology) Cells, Cultured Consortia Cytokines Erythrocytes Female Flow Cytometry Genomes Hematology Hematopoiesis Hematopoiesis - physiology Hematopoietic stem cells Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - physiology HL-60 Cells Humans Immunoenzyme Techniques Inflammation Life sciences Lymphoid cells Medicine Medicine and Health Sciences Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Fluorescence Neoplasm Proteins - physiology Parkinson's disease Peripheral blood Progenitor cells PU.1 protein Real-Time Polymerase Chain Reaction Regulators Research and Analysis Methods Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Stem cells Transcription |
| title | TC1(C8orf4) regulates hematopoietic stem/progenitor cells and hematopoiesis |
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