Role of Streptococcus pneumoniae OM001 operon in capsular polysaccharide production, virulence and survival in human saliva
Streptococcus pneumoniae is the leading cause of community-acquired pneumonia in all ages worldwide, and with ever-increasing antibiotic resistance, the understanding of its pathogenesis and spread is as important as ever. Recently, we reported the presence of a Low Molecular Weight Tyrosine Phospha...
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| description | Streptococcus pneumoniae is the leading cause of community-acquired pneumonia in all ages worldwide, and with ever-increasing antibiotic resistance, the understanding of its pathogenesis and spread is as important as ever. Recently, we reported the presence of a Low Molecular Weight Tyrosine Phosphatase (LMWPTP) Spd1837 in the pneumococcus. This protein is encoded in an operon, OM001 with two other genes, with previous work implicating this operon as important for pneumococcal virulence. Thus, we set out to investigate the role of the individual genes in the operon during pneumococcal pathogenesis. As LMWPTPs play a major role in capsular polysaccharide (CPS) biosynthesis in many bacteria, we tested the effect of mutating spd1837 and its adjacent genes, spd1836 and spd1838 on CPS levels. Our results suggest that individual deletion of the genes, including the LMWPTP, did not modulate CPS levels, in multiple conditions, and in different strain backgrounds. Following in vivo studies, Spd1836 was identified as a novel virulence factor during pneumococcal invasive disease, in both the lungs and blood, with this protein alone responsible for the effects of operon's role in virulence. We also showed that a deletion in spd1836, spd1838 or the overall OM001 operon reduced survival in human saliva during the conditions that mimic transmission compared to the wildtype strain. With studies suggesting that survival in human saliva may be important for transmission, this study identifies Spd1836 and Spd1838 as transmission factors, potentially facilitating the spread of the pneumococcus from person to person. Overall, this study hopes to further our understanding of the bacterial transmission that precedes disease and outbreaks. |
| doi_str_mv | 10.1371/journal.pone.0190402 |
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Recently, we reported the presence of a Low Molecular Weight Tyrosine Phosphatase (LMWPTP) Spd1837 in the pneumococcus. This protein is encoded in an operon, OM001 with two other genes, with previous work implicating this operon as important for pneumococcal virulence. Thus, we set out to investigate the role of the individual genes in the operon during pneumococcal pathogenesis. As LMWPTPs play a major role in capsular polysaccharide (CPS) biosynthesis in many bacteria, we tested the effect of mutating spd1837 and its adjacent genes, spd1836 and spd1838 on CPS levels. Our results suggest that individual deletion of the genes, including the LMWPTP, did not modulate CPS levels, in multiple conditions, and in different strain backgrounds. Following in vivo studies, Spd1836 was identified as a novel virulence factor during pneumococcal invasive disease, in both the lungs and blood, with this protein alone responsible for the effects of operon's role in virulence. We also showed that a deletion in spd1836, spd1838 or the overall OM001 operon reduced survival in human saliva during the conditions that mimic transmission compared to the wildtype strain. With studies suggesting that survival in human saliva may be important for transmission, this study identifies Spd1836 and Spd1838 as transmission factors, potentially facilitating the spread of the pneumococcus from person to person. Overall, this study hopes to further our understanding of the bacterial transmission that precedes disease and outbreaks.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0190402</identifier><identifier>PMID: 29293606</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibiotic resistance ; Antibiotics ; Bacteria ; Biology and Life Sciences ; Biosynthesis ; Cellular biology ; Coding ; Disease transmission ; Genes ; Genomes ; Health aspects ; In vivo methods and tests ; Infectious diseases ; Kinases ; Low molecular weights ; Lungs ; Medicine and Health Sciences ; Microbial drug resistance ; Molecular weight ; Outbreaks ; Pathogenesis ; Phosphatase ; Phosphorylation ; Physical Sciences ; Physiological aspects ; Pneumonia ; Protein-tyrosine-phosphatase ; Saliva ; Streptococcus infections ; Streptococcus pneumoniae ; Studies ; Survival ; Tyrosine ; Vaccines ; Virulence ; Virulence (Microbiology) ; Virulence factors</subject><ispartof>PloS one, 2018-01, Vol.13 (1), p.e0190402-e0190402</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Recently, we reported the presence of a Low Molecular Weight Tyrosine Phosphatase (LMWPTP) Spd1837 in the pneumococcus. This protein is encoded in an operon, OM001 with two other genes, with previous work implicating this operon as important for pneumococcal virulence. Thus, we set out to investigate the role of the individual genes in the operon during pneumococcal pathogenesis. As LMWPTPs play a major role in capsular polysaccharide (CPS) biosynthesis in many bacteria, we tested the effect of mutating spd1837 and its adjacent genes, spd1836 and spd1838 on CPS levels. Our results suggest that individual deletion of the genes, including the LMWPTP, did not modulate CPS levels, in multiple conditions, and in different strain backgrounds. Following in vivo studies, Spd1836 was identified as a novel virulence factor during pneumococcal invasive disease, in both the lungs and blood, with this protein alone responsible for the effects of operon's role in virulence. We also showed that a deletion in spd1836, spd1838 or the overall OM001 operon reduced survival in human saliva during the conditions that mimic transmission compared to the wildtype strain. With studies suggesting that survival in human saliva may be important for transmission, this study identifies Spd1836 and Spd1838 as transmission factors, potentially facilitating the spread of the pneumococcus from person to person. 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One</addtitle><date>2018-01-02</date><risdate>2018</risdate><volume>13</volume><issue>1</issue><spage>e0190402</spage><epage>e0190402</epage><pages>e0190402-e0190402</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Streptococcus pneumoniae is the leading cause of community-acquired pneumonia in all ages worldwide, and with ever-increasing antibiotic resistance, the understanding of its pathogenesis and spread is as important as ever. 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We also showed that a deletion in spd1836, spd1838 or the overall OM001 operon reduced survival in human saliva during the conditions that mimic transmission compared to the wildtype strain. With studies suggesting that survival in human saliva may be important for transmission, this study identifies Spd1836 and Spd1838 as transmission factors, potentially facilitating the spread of the pneumococcus from person to person. Overall, this study hopes to further our understanding of the bacterial transmission that precedes disease and outbreaks.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29293606</pmid><doi>10.1371/journal.pone.0190402</doi><tpages>e0190402</tpages><orcidid>https://orcid.org/0000-0002-6346-2793</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Free; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Antibiotic resistance Antibiotics Bacteria Biology and Life Sciences Biosynthesis Cellular biology Coding Disease transmission Genes Genomes Health aspects In vivo methods and tests Infectious diseases Kinases Low molecular weights Lungs Medicine and Health Sciences Microbial drug resistance Molecular weight Outbreaks Pathogenesis Phosphatase Phosphorylation Physical Sciences Physiological aspects Pneumonia Protein-tyrosine-phosphatase Saliva Streptococcus infections Streptococcus pneumoniae Studies Survival Tyrosine Vaccines Virulence Virulence (Microbiology) Virulence factors |
| title | Role of Streptococcus pneumoniae OM001 operon in capsular polysaccharide production, virulence and survival in human saliva |
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