Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis
This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum pr...
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| Veröffentlicht in: | PloS one 2014-02, Vol.9 (2), p.e88335 |
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| creator | Ren, Wenkai Yin, Jie Wu, Miaomiao Liu, Gang Yang, Guan Xion, Yan Su, Dingding Wu, Li Li, Tiejun Chen, Shuai Duan, Jielin Yin, Yulong Wu, Guoyao |
| description | This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)-myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P |
| doi_str_mv | 10.1371/journal.pone.0088335 |
| format | Article |
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Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)-myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P<0.05). Dietary arginine or glutamine supplementation had significant (P<0.05) influence on the clinical and biochemical parameters (T-SOD, IL-17 and TNF-α) in colitis model. These results were associated with colonic NF-κB, PI3K-Akt and MLCK signaling pathways. In conclusion, arginine or glutamine could be a potential therapy for intestinal inflammatory diseases.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0088335</identifier><identifier>PMID: 24505477</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>1-Phosphatidylinositol 3-kinase ; Agriculture ; AKT protein ; Amino acids ; Amino Acids - blood ; Analysis ; Animals ; Arginine ; Arginine - therapeutic use ; Biology ; Catalase ; Catalase - metabolism ; Cellular signal transduction ; Chains ; Chemistry ; Colitis - blood ; Colitis - chemically induced ; Colitis - diet therapy ; Colitis - pathology ; Colon ; Colon - pathology ; Cytokines ; Cytokines - analysis ; Dextran ; Dextran Sulfate ; Dextrans ; Diet ; Dietary Supplements ; Female ; Glutamine ; Glutamine - therapeutic use ; Glutathione ; Glutathione peroxidase ; Humans ; Immunoblotting ; Inflammatory bowel disease ; Inflammatory bowel diseases ; Inflammatory diseases ; Interleukin 1 ; Interleukin 17 ; Interleukin 6 ; Interleukins ; Intestine ; Kinases ; MAP kinase ; Mathematical models ; Medicine ; Mice ; Muscle proteins ; Myosin ; Myosin-light-chain kinase ; NF-κB protein ; Nutrition ; Peroxidase ; Phosphatidylinositol 3-Kinases ; Protein kinases ; Proteins ; Rodents ; Signal transduction ; Signaling ; Sodium ; Sulfates ; Superoxide dismutase ; Superoxides ; Ulcerative colitis</subject><ispartof>PloS one, 2014-02, Vol.9 (2), p.e88335</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Ren et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Ren et al 2014 Ren et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-938b3baedc287261cf7886ab8d28393158f2be48665487ea84c20a4ef6cb4bc23</citedby><cites>FETCH-LOGICAL-c758t-938b3baedc287261cf7886ab8d28393158f2be48665487ea84c20a4ef6cb4bc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914992/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914992/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23870,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24505477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Thi Thu Nguyen, Hang</contributor><creatorcontrib>Ren, Wenkai</creatorcontrib><creatorcontrib>Yin, Jie</creatorcontrib><creatorcontrib>Wu, Miaomiao</creatorcontrib><creatorcontrib>Liu, Gang</creatorcontrib><creatorcontrib>Yang, Guan</creatorcontrib><creatorcontrib>Xion, Yan</creatorcontrib><creatorcontrib>Su, Dingding</creatorcontrib><creatorcontrib>Wu, Li</creatorcontrib><creatorcontrib>Li, Tiejun</creatorcontrib><creatorcontrib>Chen, Shuai</creatorcontrib><creatorcontrib>Duan, Jielin</creatorcontrib><creatorcontrib>Yin, Yulong</creatorcontrib><creatorcontrib>Wu, Guoyao</creatorcontrib><title>Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)-myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P<0.05). Dietary arginine or glutamine supplementation had significant (P<0.05) influence on the clinical and biochemical parameters (T-SOD, IL-17 and TNF-α) in colitis model. These results were associated with colonic NF-κB, PI3K-Akt and MLCK signaling pathways. In conclusion, arginine or glutamine could be a potential therapy for intestinal inflammatory diseases.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>Agriculture</subject><subject>AKT protein</subject><subject>Amino acids</subject><subject>Amino Acids - blood</subject><subject>Analysis</subject><subject>Animals</subject><subject>Arginine</subject><subject>Arginine - therapeutic use</subject><subject>Biology</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Cellular signal transduction</subject><subject>Chains</subject><subject>Chemistry</subject><subject>Colitis - blood</subject><subject>Colitis - chemically induced</subject><subject>Colitis - diet therapy</subject><subject>Colitis - pathology</subject><subject>Colon</subject><subject>Colon - pathology</subject><subject>Cytokines</subject><subject>Cytokines - analysis</subject><subject>Dextran</subject><subject>Dextran Sulfate</subject><subject>Dextrans</subject><subject>Diet</subject><subject>Dietary Supplements</subject><subject>Female</subject><subject>Glutamine</subject><subject>Glutamine - therapeutic use</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Inflammatory bowel disease</subject><subject>Inflammatory bowel diseases</subject><subject>Inflammatory diseases</subject><subject>Interleukin 1</subject><subject>Interleukin 17</subject><subject>Interleukin 6</subject><subject>Interleukins</subject><subject>Intestine</subject><subject>Kinases</subject><subject>MAP kinase</subject><subject>Mathematical models</subject><subject>Medicine</subject><subject>Mice</subject><subject>Muscle proteins</subject><subject>Myosin</subject><subject>Myosin-light-chain kinase</subject><subject>NF-κB protein</subject><subject>Nutrition</subject><subject>Peroxidase</subject><subject>Phosphatidylinositol 3-Kinases</subject><subject>Protein kinases</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Sodium</subject><subject>Sulfates</subject><subject>Superoxide dismutase</subject><subject>Superoxides</subject><subject>Ulcerative 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amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis</title><author>Ren, Wenkai ; Yin, Jie ; Wu, Miaomiao ; Liu, Gang ; Yang, Guan ; Xion, Yan ; Su, Dingding ; Wu, Li ; Li, Tiejun ; Chen, Shuai ; Duan, Jielin ; Yin, Yulong ; Wu, Guoyao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-938b3baedc287261cf7886ab8d28393158f2be48665487ea84c20a4ef6cb4bc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>Agriculture</topic><topic>AKT protein</topic><topic>Amino acids</topic><topic>Amino Acids - blood</topic><topic>Analysis</topic><topic>Animals</topic><topic>Arginine</topic><topic>Arginine - therapeutic use</topic><topic>Biology</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Cellular signal 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Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Wenkai</au><au>Yin, Jie</au><au>Wu, Miaomiao</au><au>Liu, Gang</au><au>Yang, Guan</au><au>Xion, Yan</au><au>Su, Dingding</au><au>Wu, Li</au><au>Li, Tiejun</au><au>Chen, Shuai</au><au>Duan, Jielin</au><au>Yin, Yulong</au><au>Wu, Guoyao</au><au>Thi Thu Nguyen, Hang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-02-05</date><risdate>2014</risdate><volume>9</volume><issue>2</issue><spage>e88335</spage><pages>e88335-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)-myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P<0.05). Dietary arginine or glutamine supplementation had significant (P<0.05) influence on the clinical and biochemical parameters (T-SOD, IL-17 and TNF-α) in colitis model. These results were associated with colonic NF-κB, PI3K-Akt and MLCK signaling pathways. In conclusion, arginine or glutamine could be a potential therapy for intestinal inflammatory diseases.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24505477</pmid><doi>10.1371/journal.pone.0088335</doi><tpages>e88335</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-02, Vol.9 (2), p.e88335 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1983418390 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 1-Phosphatidylinositol 3-kinase Agriculture AKT protein Amino acids Amino Acids - blood Analysis Animals Arginine Arginine - therapeutic use Biology Catalase Catalase - metabolism Cellular signal transduction Chains Chemistry Colitis - blood Colitis - chemically induced Colitis - diet therapy Colitis - pathology Colon Colon - pathology Cytokines Cytokines - analysis Dextran Dextran Sulfate Dextrans Diet Dietary Supplements Female Glutamine Glutamine - therapeutic use Glutathione Glutathione peroxidase Humans Immunoblotting Inflammatory bowel disease Inflammatory bowel diseases Inflammatory diseases Interleukin 1 Interleukin 17 Interleukin 6 Interleukins Intestine Kinases MAP kinase Mathematical models Medicine Mice Muscle proteins Myosin Myosin-light-chain kinase NF-κB protein Nutrition Peroxidase Phosphatidylinositol 3-Kinases Protein kinases Proteins Rodents Signal transduction Signaling Sodium Sulfates Superoxide dismutase Superoxides Ulcerative colitis |
| title | Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis |
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