Detection of rotavirus using padlock probes and rolling circle amplification
Rotavirus infections are one of the most common reasons for hospitalizations due to gastrointestinal diseases. Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain rea...
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| Veröffentlicht in: | PloS one 2014, Vol.9 (11), p.e111874-e111874 |
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| creator | Mezger, Anja Öhrmalm, Christina Herthnek, David Blomberg, Jonas Nilsson, Mats |
| description | Rotavirus infections are one of the most common reasons for hospitalizations due to gastrointestinal diseases. Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. In summary, we present a new assay for sensitive and variation tolerant detection of rotavirus. |
| doi_str_mv | 10.1371/journal.pone.0111874 |
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Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. In summary, we present a new assay for sensitive and variation tolerant detection of rotavirus.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0111874</identifier><identifier>PMID: 25369034</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Agglutination ; Base Sequence ; Biochemistry ; Biology and life sciences ; Biophysics ; Chromatography ; Design ; Disease ; DNA probes ; DNA Probes - genetics ; Electron microscopy ; Enzymes ; Feces ; Gastrointestinal diseases ; Humans ; Immunoassay ; Laboratories ; Latex ; Latex agglutination ; Limit of Detection ; Microscopy ; Molecular Diagnostic Techniques ; Molecular Sequence Data ; National libraries ; Nucleic Acid Amplification Techniques ; Polymerase chain reaction ; Probes ; Research and Analysis Methods ; Reverse transcription ; Rotavirus ; Rotavirus - genetics ; Rotavirus Infections - diagnosis ; Rotavirus Infections - virology ; Science ; Target recognition ; Vaccines ; Virology ; Viruses</subject><ispartof>PloS one, 2014, Vol.9 (11), p.e111874-e111874</ispartof><rights>2014 Mezger et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. 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Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. In summary, we present a new assay for sensitive and variation tolerant detection of rotavirus.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25369034</pmid><doi>10.1371/journal.pone.0111874</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Agglutination Base Sequence Biochemistry Biology and life sciences Biophysics Chromatography Design Disease DNA probes DNA Probes - genetics Electron microscopy Enzymes Feces Gastrointestinal diseases Humans Immunoassay Laboratories Latex Latex agglutination Limit of Detection Microscopy Molecular Diagnostic Techniques Molecular Sequence Data National libraries Nucleic Acid Amplification Techniques Polymerase chain reaction Probes Research and Analysis Methods Reverse transcription Rotavirus Rotavirus - genetics Rotavirus Infections - diagnosis Rotavirus Infections - virology Science Target recognition Vaccines Virology Viruses |
| title | Detection of rotavirus using padlock probes and rolling circle amplification |
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