A novel bifunctional hybrid with marine bacterium alkaline phosphatase and Far Eastern holothurian mannan-binding lectin activities

A fusion between the genes encoding the marine bacterium Cobetia marina alkaline phosphatase (CmAP) and Far Eastern holothurian Apostichopus japonicus mannan-binding C-type lectin (MBL-AJ) was performed. Expression of the fusion gene in E. coli cells resulted in yield of soluble recombinant chimeric...

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Veröffentlicht in:	PloS one 2014-11, Vol.9 (11), p.e112729
Hauptverfasser:	Balabanova, Larissa, Golotin, Vasily, Kovalchuk, Svetlana, Bulgakov, Alexander, Likhatskaya, Galina, Son, Oksana, Rasskazov, Valery
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline phosphatase Alkaline Phosphatase - chemistry Alkaline Phosphatase - genetics Amino Acid Substitution - genetics Analysis Animals Antigens Bacteria Binding Biochemistry Biology and Life Sciences Biomarkers Carbohydrates Cervical cancer Chemical properties Chromatography, Gel Cloning Computer and Information Sciences Deoxyribonucleic acid Dimerization DNA DNA Primers - genetics E coli Engineering and Technology Escherichia coli Fusion protein Gene expression Halomonadaceae - enzymology Laboratories Lectins Ligands Mannan Mannose-binding lectin Mannose-Binding Lectin - chemistry Mannose-Binding Lectin - genetics Medical screening Models, Molecular Mutagenesis, Site-Directed Phosphatase Phosphatases Plasmids Plasmids - genetics Protein Conformation Proteins Quantum dots Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sea Cucumbers - genetics
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