L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets
L-Glutamate is a major oxidative fuel for the small intestine. However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementat...
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| creator | Lin, Meng Zhang, Bolin Yu, Changning Li, Jiaolong Zhang, Lin Sun, Hui Gao, Feng Zhou, Guanghong |
| description | L-Glutamate is a major oxidative fuel for the small intestine. However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. A total of 120 weaning piglets aged 35 ± 1 days with an average body weight at 8.91 ± 0.45 kg were randomly allocated to two treatments with six replicates of ten piglets each, fed with diets containing 1.21% alanine, or 2% L-glutamate. L-Glutamate supplementation increased the activity of glutamate oxaloacetate transaminase (GOT) in the jejunal mucosa. Also, the mRNA expression level of jejunal mucosa glutamine synthetase (GS) was increased by L-glutamate supplementation. The height of villi in duodenal and jejunal segments, and the relative mRNA expression of occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa were increased by dietary L-glutamate supplementation. L-Glutamate supplementation increased plasma concentrations of glutamate, arginine, histidine, isoleucine, leucine, methionine, phenylalanine and threonine. L-Glutamate supplementation also increased the relative mRNA expression of the jejunal mucosa Ca(2+)-sensing receptor (CaR), metabotropic glutamate receptor 1 (mGluR1) and metabotropic glutamate receptor 4 (mGluR4), and neutral amino acid transporter B(0)-like (SLC1A5) in the jejunal mucosa. These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption. |
| doi_str_mv | 10.1371/journal.pone.0111950 |
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However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. A total of 120 weaning piglets aged 35 ± 1 days with an average body weight at 8.91 ± 0.45 kg were randomly allocated to two treatments with six replicates of ten piglets each, fed with diets containing 1.21% alanine, or 2% L-glutamate. L-Glutamate supplementation increased the activity of glutamate oxaloacetate transaminase (GOT) in the jejunal mucosa. Also, the mRNA expression level of jejunal mucosa glutamine synthetase (GS) was increased by L-glutamate supplementation. The height of villi in duodenal and jejunal segments, and the relative mRNA expression of occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa were increased by dietary L-glutamate supplementation. L-Glutamate supplementation increased plasma concentrations of glutamate, arginine, histidine, isoleucine, leucine, methionine, phenylalanine and threonine. L-Glutamate supplementation also increased the relative mRNA expression of the jejunal mucosa Ca(2+)-sensing receptor (CaR), metabotropic glutamate receptor 1 (mGluR1) and metabotropic glutamate receptor 4 (mGluR4), and neutral amino acid transporter B(0)-like (SLC1A5) in the jejunal mucosa. These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0111950</identifier><identifier>PMID: 25368996</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alanine ; Amino Acid Transport Systems - metabolism ; Amino acids ; Amino Acids - blood ; Animal sciences ; Animals ; Architecture ; Arginine ; Aspartate Aminotransferases - metabolism ; Biology and Life Sciences ; Body weight ; Calcium ; Claudin-1 - genetics ; Claudin-1 - metabolism ; Diet ; Dietary Supplements ; Food safety ; Gene Expression ; Glutamate-ammonia ligase ; Glutamic acid ; Glutamic Acid - administration & dosage ; Glutamic acid receptors (metabotropic) ; Glutamine ; Histidine ; Hogs ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; Isoleucine ; Jejunum ; Jejunum - cytology ; Jejunum - metabolism ; Laboratories ; Leucine ; Male ; Metabolism ; Methionine ; Mucosa ; Nutrients ; Nutrition ; Occludin - genetics ; Occludin - metabolism ; Parenteral nutrition ; Phenylalanine ; Physiology ; Proteins ; Receptors ; Rodents ; Signaling ; Small intestine ; Studies ; Sus scrofa ; Threonine ; Transaminase ; Weaning ; Zonula Occludens-1 Protein - genetics ; Zonula Occludens-1 Protein - metabolism ; Zoology</subject><ispartof>PloS one, 2014-11, Vol.9 (11), p.e111950-e111950</ispartof><rights>2014 Lin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Lin et al 2014 Lin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-104eb02abe704d7b12ae882d3d45d57c3918a6047da6a2e7119437c1aa7fd7063</citedby><cites>FETCH-LOGICAL-c592t-104eb02abe704d7b12ae882d3d45d57c3918a6047da6a2e7119437c1aa7fd7063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219819/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219819/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25368996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Meng</creatorcontrib><creatorcontrib>Zhang, Bolin</creatorcontrib><creatorcontrib>Yu, Changning</creatorcontrib><creatorcontrib>Li, Jiaolong</creatorcontrib><creatorcontrib>Zhang, Lin</creatorcontrib><creatorcontrib>Sun, Hui</creatorcontrib><creatorcontrib>Gao, Feng</creatorcontrib><creatorcontrib>Zhou, Guanghong</creatorcontrib><title>L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>L-Glutamate is a major oxidative fuel for the small intestine. However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. A total of 120 weaning piglets aged 35 ± 1 days with an average body weight at 8.91 ± 0.45 kg were randomly allocated to two treatments with six replicates of ten piglets each, fed with diets containing 1.21% alanine, or 2% L-glutamate. L-Glutamate supplementation increased the activity of glutamate oxaloacetate transaminase (GOT) in the jejunal mucosa. Also, the mRNA expression level of jejunal mucosa glutamine synthetase (GS) was increased by L-glutamate supplementation. The height of villi in duodenal and jejunal segments, and the relative mRNA expression of occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa were increased by dietary L-glutamate supplementation. L-Glutamate supplementation increased plasma concentrations of glutamate, arginine, histidine, isoleucine, leucine, methionine, phenylalanine and threonine. L-Glutamate supplementation also increased the relative mRNA expression of the jejunal mucosa Ca(2+)-sensing receptor (CaR), metabotropic glutamate receptor 1 (mGluR1) and metabotropic glutamate receptor 4 (mGluR4), and neutral amino acid transporter B(0)-like (SLC1A5) in the jejunal mucosa. These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.</description><subject>Alanine</subject><subject>Amino Acid Transport Systems - metabolism</subject><subject>Amino acids</subject><subject>Amino Acids - blood</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Architecture</subject><subject>Arginine</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Body weight</subject><subject>Calcium</subject><subject>Claudin-1 - genetics</subject><subject>Claudin-1 - metabolism</subject><subject>Diet</subject><subject>Dietary Supplements</subject><subject>Food safety</subject><subject>Gene Expression</subject><subject>Glutamate-ammonia ligase</subject><subject>Glutamic acid</subject><subject>Glutamic Acid - administration & dosage</subject><subject>Glutamic acid receptors (metabotropic)</subject><subject>Glutamine</subject><subject>Histidine</subject><subject>Hogs</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Isoleucine</subject><subject>Jejunum</subject><subject>Jejunum - cytology</subject><subject>Jejunum - metabolism</subject><subject>Laboratories</subject><subject>Leucine</subject><subject>Male</subject><subject>Metabolism</subject><subject>Methionine</subject><subject>Mucosa</subject><subject>Nutrients</subject><subject>Nutrition</subject><subject>Occludin - genetics</subject><subject>Occludin - metabolism</subject><subject>Parenteral nutrition</subject><subject>Phenylalanine</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Rodents</subject><subject>Signaling</subject><subject>Small intestine</subject><subject>Studies</subject><subject>Sus scrofa</subject><subject>Threonine</subject><subject>Transaminase</subject><subject>Weaning</subject><subject>Zonula Occludens-1 Protein - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Meng</au><au>Zhang, Bolin</au><au>Yu, Changning</au><au>Li, Jiaolong</au><au>Zhang, Lin</au><au>Sun, Hui</au><au>Gao, Feng</au><au>Zhou, Guanghong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-11-04</date><risdate>2014</risdate><volume>9</volume><issue>11</issue><spage>e111950</spage><epage>e111950</epage><pages>e111950-e111950</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>L-Glutamate is a major oxidative fuel for the small intestine. However, few studies have demonstrated the effect of L-glutamate on the intestinal architecture and signaling of amino acids in the small intestine. The aim of this study was to investigate the effects of dietary L-glutamate supplementation on the intestinal architecture and expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. A total of 120 weaning piglets aged 35 ± 1 days with an average body weight at 8.91 ± 0.45 kg were randomly allocated to two treatments with six replicates of ten piglets each, fed with diets containing 1.21% alanine, or 2% L-glutamate. L-Glutamate supplementation increased the activity of glutamate oxaloacetate transaminase (GOT) in the jejunal mucosa. Also, the mRNA expression level of jejunal mucosa glutamine synthetase (GS) was increased by L-glutamate supplementation. The height of villi in duodenal and jejunal segments, and the relative mRNA expression of occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa were increased by dietary L-glutamate supplementation. L-Glutamate supplementation increased plasma concentrations of glutamate, arginine, histidine, isoleucine, leucine, methionine, phenylalanine and threonine. L-Glutamate supplementation also increased the relative mRNA expression of the jejunal mucosa Ca(2+)-sensing receptor (CaR), metabotropic glutamate receptor 1 (mGluR1) and metabotropic glutamate receptor 4 (mGluR4), and neutral amino acid transporter B(0)-like (SLC1A5) in the jejunal mucosa. These findings suggest that dietary addition of 2% L-glutamate improves the intestinal integrity and influences the expression of amino acid receptors and transporters in the jejunum of weaning, which is beneficial for the improvement of jejunal nutrients for digestion and absorption.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25368996</pmid><doi>10.1371/journal.pone.0111950</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1979937147 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Alanine Amino Acid Transport Systems - metabolism Amino acids Amino Acids - blood Animal sciences Animals Architecture Arginine Aspartate Aminotransferases - metabolism Biology and Life Sciences Body weight Calcium Claudin-1 - genetics Claudin-1 - metabolism Diet Dietary Supplements Food safety Gene Expression Glutamate-ammonia ligase Glutamic acid Glutamic Acid - administration & dosage Glutamic acid receptors (metabotropic) Glutamine Histidine Hogs Intestinal Mucosa - cytology Intestinal Mucosa - metabolism Isoleucine Jejunum Jejunum - cytology Jejunum - metabolism Laboratories Leucine Male Metabolism Methionine Mucosa Nutrients Nutrition Occludin - genetics Occludin - metabolism Parenteral nutrition Phenylalanine Physiology Proteins Receptors Rodents Signaling Small intestine Studies Sus scrofa Threonine Transaminase Weaning Zonula Occludens-1 Protein - genetics Zonula Occludens-1 Protein - metabolism Zoology |
| title | L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T01%3A32%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=L-Glutamate%20supplementation%20improves%20small%20intestinal%20architecture%20and%20enhances%20the%20expressions%20of%20jejunal%20mucosa%20amino%20acid%20receptors%20and%20transporters%20in%20weaning%20piglets&rft.jtitle=PloS%20one&rft.au=Lin,%20Meng&rft.date=2014-11-04&rft.volume=9&rft.issue=11&rft.spage=e111950&rft.epage=e111950&rft.pages=e111950-e111950&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0111950&rft_dat=%3Cproquest_plos_%3E1979937147%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1979937147&rft_id=info:pmid/25368996&rft_doaj_id=oai_doaj_org_article_d718f3dee95a4729890741c1d0d6bf1c&rfr_iscdi=true |