Genotype distribution, viral load and clinical characteristics of infants with postnatal or congenital cytomegalovirus infection
Congenital cytomegalovirus infection is a leading cause of long-term sequelae. Cytomegalovirus is also frequently transmitted to preterm infants postnatally, but these infections are mostly asymptomatic. A correlation between cytomegalovirus genotypes and clinical manifestations has been reported pr...
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| creator | Nijman, Joppe Mandemaker, Femke S Verboon-Maciolek, Malgorzata A Aitken, Susan C van Loon, Anton M de Vries, Linda S Schuurman, Rob |
| description | Congenital cytomegalovirus infection is a leading cause of long-term sequelae. Cytomegalovirus is also frequently transmitted to preterm infants postnatally, but these infections are mostly asymptomatic. A correlation between cytomegalovirus genotypes and clinical manifestations has been reported previously in infants with congenital infection, but not in preterm infants with postnatal infection.
The main objective of this study was to investigate cytomegalovirus genotype distribution in postnatal and congenital cytomegalovirus infection and its association with disease severity.
Infants admitted to the neonatal intensive care unit of the University Medical Center Utrecht, The Netherlands between 2003-2010 and diagnosed with postnatal or congenital cytomegalovirus infection were included. Classification of cytomegalovirus isolates in genotypes was performed upon amplification and sequencing of the cytomegalovirus UL55 (gB) and UL144 genes. Clinical data, cerebral abnormalities, neurodevelopmental outcome and viral load were studied in relation to genotype distribution.
Genotyping results were obtained from 58 preterm infants with postnatal cytomegalovirus infection and 13 infants with congenital cytomegalovirus infection. Postnatal disease was mild in all preterm infants and all had favourable outcome. Infants with congenital infection were significantly more severely affected than infants with postnatal infection. Seventy-seven percent of these infants were symptomatic at birth, 2/13 died and 3/13 developed long-term sequelae (median follow-up 6 (range 2-8) years). The distribution of cytomegalovirus genotypes was comparable for postnatal and congenital infection. UL55 genotype 1 and UL144 genotype 3 were predominant genotypes in both groups.
Distribution of UL55 and UL144 genotypes was similar in asymptomatic postnatal and severe congenital CMV infection suggesting that other factors rather than cytomegalovirus UL55 and UL144 genotype are responsible for the development of severe disease. |
| doi_str_mv | 10.1371/journal.pone.0108018 |
| format | Article |
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The main objective of this study was to investigate cytomegalovirus genotype distribution in postnatal and congenital cytomegalovirus infection and its association with disease severity.
Infants admitted to the neonatal intensive care unit of the University Medical Center Utrecht, The Netherlands between 2003-2010 and diagnosed with postnatal or congenital cytomegalovirus infection were included. Classification of cytomegalovirus isolates in genotypes was performed upon amplification and sequencing of the cytomegalovirus UL55 (gB) and UL144 genes. Clinical data, cerebral abnormalities, neurodevelopmental outcome and viral load were studied in relation to genotype distribution.
Genotyping results were obtained from 58 preterm infants with postnatal cytomegalovirus infection and 13 infants with congenital cytomegalovirus infection. Postnatal disease was mild in all preterm infants and all had favourable outcome. Infants with congenital infection were significantly more severely affected than infants with postnatal infection. Seventy-seven percent of these infants were symptomatic at birth, 2/13 died and 3/13 developed long-term sequelae (median follow-up 6 (range 2-8) years). The distribution of cytomegalovirus genotypes was comparable for postnatal and congenital infection. UL55 genotype 1 and UL144 genotype 3 were predominant genotypes in both groups.
Distribution of UL55 and UL144 genotypes was similar in asymptomatic postnatal and severe congenital CMV infection suggesting that other factors rather than cytomegalovirus UL55 and UL144 genotype are responsible for the development of severe disease.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0108018</identifier><identifier>PMID: 25268349</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abnormalities ; Age ; Biology and Life Sciences ; Child ; Child, Preschool ; Complications ; Congenital diseases ; Congenital infection ; Cross Infection - pathology ; Cross Infection - virology ; Cytomegalovirus ; Cytomegalovirus - classification ; Cytomegalovirus - genetics ; Cytomegalovirus - isolation & purification ; Cytomegalovirus Infections - congenital ; Cytomegalovirus Infections - mortality ; Cytomegalovirus Infections - pathology ; Cytomegalovirus Infections - virology ; Deoxyribonucleic acid ; DNA ; Female ; Gene sequencing ; Genotype ; Genotype & phenotype ; Genotypes ; Genotyping ; Health care facilities ; Health risk assessment ; Hearing loss ; Humans ; Infant ; Infant, Newborn ; Infant, Premature ; Infants ; Infections ; Intensive care ; Load distribution ; Load distribution (forces) ; Longitudinal Studies ; Male ; Medicine and Health Sciences ; Membrane Glycoproteins - genetics ; Neonates ; Netherlands ; Neurodevelopmental disorders ; Newborn babies ; NMR ; Nuclear magnetic resonance ; Pneumonia ; Polymerase chain reaction ; Postnatal infection ; Sepsis ; Severity of Illness Index ; Stress concentration ; Studies ; Survival Analysis ; Ultrasonic imaging ; Urine ; Viral Envelope Proteins ; Viral Load ; Viral Proteins - genetics ; Virology</subject><ispartof>PloS one, 2014-09, Vol.9 (9), p.e108018-e108018</ispartof><rights>2014 Nijman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Nijman et al 2014 Nijman et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-e5706024c6521a7b02088a4819ec6bbd4f8d151d2e599007594aa435e4cfba203</citedby><cites>FETCH-LOGICAL-c526t-e5706024c6521a7b02088a4819ec6bbd4f8d151d2e599007594aa435e4cfba203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182318/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182318/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25268349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gantt, Soren</contributor><creatorcontrib>Nijman, Joppe</creatorcontrib><creatorcontrib>Mandemaker, Femke S</creatorcontrib><creatorcontrib>Verboon-Maciolek, Malgorzata A</creatorcontrib><creatorcontrib>Aitken, Susan C</creatorcontrib><creatorcontrib>van Loon, Anton M</creatorcontrib><creatorcontrib>de Vries, Linda S</creatorcontrib><creatorcontrib>Schuurman, Rob</creatorcontrib><title>Genotype distribution, viral load and clinical characteristics of infants with postnatal or congenital cytomegalovirus infection</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Congenital cytomegalovirus infection is a leading cause of long-term sequelae. Cytomegalovirus is also frequently transmitted to preterm infants postnatally, but these infections are mostly asymptomatic. A correlation between cytomegalovirus genotypes and clinical manifestations has been reported previously in infants with congenital infection, but not in preterm infants with postnatal infection.
The main objective of this study was to investigate cytomegalovirus genotype distribution in postnatal and congenital cytomegalovirus infection and its association with disease severity.
Infants admitted to the neonatal intensive care unit of the University Medical Center Utrecht, The Netherlands between 2003-2010 and diagnosed with postnatal or congenital cytomegalovirus infection were included. Classification of cytomegalovirus isolates in genotypes was performed upon amplification and sequencing of the cytomegalovirus UL55 (gB) and UL144 genes. Clinical data, cerebral abnormalities, neurodevelopmental outcome and viral load were studied in relation to genotype distribution.
Genotyping results were obtained from 58 preterm infants with postnatal cytomegalovirus infection and 13 infants with congenital cytomegalovirus infection. Postnatal disease was mild in all preterm infants and all had favourable outcome. Infants with congenital infection were significantly more severely affected than infants with postnatal infection. Seventy-seven percent of these infants were symptomatic at birth, 2/13 died and 3/13 developed long-term sequelae (median follow-up 6 (range 2-8) years). The distribution of cytomegalovirus genotypes was comparable for postnatal and congenital infection. UL55 genotype 1 and UL144 genotype 3 were predominant genotypes in both groups.
Distribution of UL55 and UL144 genotypes was similar in asymptomatic postnatal and severe congenital CMV infection suggesting that other factors rather than cytomegalovirus UL55 and UL144 genotype are responsible for the development of severe disease.</description><subject>Abnormalities</subject><subject>Age</subject><subject>Biology and Life Sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Complications</subject><subject>Congenital diseases</subject><subject>Congenital infection</subject><subject>Cross Infection - pathology</subject><subject>Cross Infection - virology</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - classification</subject><subject>Cytomegalovirus - genetics</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>Cytomegalovirus Infections - congenital</subject><subject>Cytomegalovirus Infections - mortality</subject><subject>Cytomegalovirus Infections - pathology</subject><subject>Cytomegalovirus Infections - virology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Health care facilities</subject><subject>Health risk assessment</subject><subject>Hearing loss</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infant, Premature</subject><subject>Infants</subject><subject>Infections</subject><subject>Intensive care</subject><subject>Load distribution</subject><subject>Load distribution (forces)</subject><subject>Longitudinal Studies</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Neonates</subject><subject>Netherlands</subject><subject>Neurodevelopmental disorders</subject><subject>Newborn babies</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Pneumonia</subject><subject>Polymerase chain reaction</subject><subject>Postnatal infection</subject><subject>Sepsis</subject><subject>Severity of Illness Index</subject><subject>Stress concentration</subject><subject>Studies</subject><subject>Survival Analysis</subject><subject>Ultrasonic imaging</subject><subject>Urine</subject><subject>Viral Envelope Proteins</subject><subject>Viral Load</subject><subject>Viral Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nijman, Joppe</au><au>Mandemaker, Femke S</au><au>Verboon-Maciolek, Malgorzata A</au><au>Aitken, Susan C</au><au>van Loon, Anton M</au><au>de Vries, Linda S</au><au>Schuurman, Rob</au><au>Gantt, Soren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotype distribution, viral load and clinical characteristics of infants with postnatal or congenital cytomegalovirus infection</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-09-30</date><risdate>2014</risdate><volume>9</volume><issue>9</issue><spage>e108018</spage><epage>e108018</epage><pages>e108018-e108018</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Congenital cytomegalovirus infection is a leading cause of long-term sequelae. Cytomegalovirus is also frequently transmitted to preterm infants postnatally, but these infections are mostly asymptomatic. A correlation between cytomegalovirus genotypes and clinical manifestations has been reported previously in infants with congenital infection, but not in preterm infants with postnatal infection.
The main objective of this study was to investigate cytomegalovirus genotype distribution in postnatal and congenital cytomegalovirus infection and its association with disease severity.
Infants admitted to the neonatal intensive care unit of the University Medical Center Utrecht, The Netherlands between 2003-2010 and diagnosed with postnatal or congenital cytomegalovirus infection were included. Classification of cytomegalovirus isolates in genotypes was performed upon amplification and sequencing of the cytomegalovirus UL55 (gB) and UL144 genes. Clinical data, cerebral abnormalities, neurodevelopmental outcome and viral load were studied in relation to genotype distribution.
Genotyping results were obtained from 58 preterm infants with postnatal cytomegalovirus infection and 13 infants with congenital cytomegalovirus infection. Postnatal disease was mild in all preterm infants and all had favourable outcome. Infants with congenital infection were significantly more severely affected than infants with postnatal infection. Seventy-seven percent of these infants were symptomatic at birth, 2/13 died and 3/13 developed long-term sequelae (median follow-up 6 (range 2-8) years). The distribution of cytomegalovirus genotypes was comparable for postnatal and congenital infection. UL55 genotype 1 and UL144 genotype 3 were predominant genotypes in both groups.
Distribution of UL55 and UL144 genotypes was similar in asymptomatic postnatal and severe congenital CMV infection suggesting that other factors rather than cytomegalovirus UL55 and UL144 genotype are responsible for the development of severe disease.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25268349</pmid><doi>10.1371/journal.pone.0108018</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1979819942 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abnormalities Age Biology and Life Sciences Child Child, Preschool Complications Congenital diseases Congenital infection Cross Infection - pathology Cross Infection - virology Cytomegalovirus Cytomegalovirus - classification Cytomegalovirus - genetics Cytomegalovirus - isolation & purification Cytomegalovirus Infections - congenital Cytomegalovirus Infections - mortality Cytomegalovirus Infections - pathology Cytomegalovirus Infections - virology Deoxyribonucleic acid DNA Female Gene sequencing Genotype Genotype & phenotype Genotypes Genotyping Health care facilities Health risk assessment Hearing loss Humans Infant Infant, Newborn Infant, Premature Infants Infections Intensive care Load distribution Load distribution (forces) Longitudinal Studies Male Medicine and Health Sciences Membrane Glycoproteins - genetics Neonates Netherlands Neurodevelopmental disorders Newborn babies NMR Nuclear magnetic resonance Pneumonia Polymerase chain reaction Postnatal infection Sepsis Severity of Illness Index Stress concentration Studies Survival Analysis Ultrasonic imaging Urine Viral Envelope Proteins Viral Load Viral Proteins - genetics Virology |
| title | Genotype distribution, viral load and clinical characteristics of infants with postnatal or congenital cytomegalovirus infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T20%3A56%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genotype%20distribution,%20viral%20load%20and%20clinical%20characteristics%20of%20infants%20with%20postnatal%20or%20congenital%20cytomegalovirus%20infection&rft.jtitle=PloS%20one&rft.au=Nijman,%20Joppe&rft.date=2014-09-30&rft.volume=9&rft.issue=9&rft.spage=e108018&rft.epage=e108018&rft.pages=e108018-e108018&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0108018&rft_dat=%3Cproquest_plos_%3E1979819942%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1979819942&rft_id=info:pmid/25268349&rft_doaj_id=oai_doaj_org_article_24567823c4e24fb8a0d1fdb605be8d34&rfr_iscdi=true |