Evaluation of an automated rapid diagnostic assay for detection of Gram-negative bacteria and their drug-resistance genes in positive blood cultures
We evaluated the performance of the Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN; Nanosphere, Northbrook, IL, USA), an automated multiplex assay for rapid identification of positive blood cultures caused by 9 Gram-negative bacteria (GNB) and for detection of 9 genes associated with...
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| Veröffentlicht in: | PloS one 2014-04, Vol.9 (4), p.e94064-e94064 |
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| creator | Tojo, Masayoshi Fujita, Takahiro Ainoda, Yusuke Nagamatsu, Maki Hayakawa, Kayoko Mezaki, Kazuhisa Sakurai, Aki Masui, Yoshinori Yazaki, Hirohisa Takahashi, Hiroshi Miyoshi-Akiyama, Tohru Totsuka, Kyoichi Kirikae, Teruo Ohmagari, Norio |
| description | We evaluated the performance of the Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN; Nanosphere, Northbrook, IL, USA), an automated multiplex assay for rapid identification of positive blood cultures caused by 9 Gram-negative bacteria (GNB) and for detection of 9 genes associated with β-lactam resistance. The BC-GN assay can be performed directly from positive blood cultures with 5 minutes of hands-on and 2 hours of run time per sample. A total of 397 GNB positive blood cultures were analyzed using the BC-GN assay. Of the 397 samples, 295 were simulated samples prepared by inoculating GNB into blood culture bottles, and the remaining were clinical samples from 102 patients with positive blood cultures. Aliquots of the positive blood cultures were tested by the BC-GN assay. The results of bacterial identification between the BC-GN assay and standard laboratory methods were as follows: Acinetobacter spp. (39 isolates for the BC-GN assay/39 for the standard methods), Citrobacter spp. (7/7), Escherichia coli (87/87), Klebsiella oxytoca (13/13), and Proteus spp. (11/11); Enterobacter spp. (29/30); Klebsiella pneumoniae (62/72); Pseudomonas aeruginosa (124/125); and Serratia marcescens (18/21); respectively. From the 102 clinical samples, 104 bacterial species were identified with the BC-GN assay, whereas 110 were identified with the standard methods. The BC-GN assay also detected all β-lactam resistance genes tested (233 genes), including 54 bla(CTX-M), 119 bla(IMP), 8 bla(KPC), 16 bla(NDM), 24 bla(OXA-23), 1 bla(OXA-24/40), 1 bla(OXA-48), 4 bla(OXA-58), and 6 blaVIM. The data shows that the BC-GN assay provides rapid detection of GNB and β-lactam resistance genes in positive blood cultures and has the potential to contributing to optimal patient management by earlier detection of major antimicrobial resistance genes. |
| doi_str_mv | 10.1371/journal.pone.0094064 |
| format | Article |
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The BC-GN assay can be performed directly from positive blood cultures with 5 minutes of hands-on and 2 hours of run time per sample. A total of 397 GNB positive blood cultures were analyzed using the BC-GN assay. Of the 397 samples, 295 were simulated samples prepared by inoculating GNB into blood culture bottles, and the remaining were clinical samples from 102 patients with positive blood cultures. Aliquots of the positive blood cultures were tested by the BC-GN assay. The results of bacterial identification between the BC-GN assay and standard laboratory methods were as follows: Acinetobacter spp. (39 isolates for the BC-GN assay/39 for the standard methods), Citrobacter spp. (7/7), Escherichia coli (87/87), Klebsiella oxytoca (13/13), and Proteus spp. (11/11); Enterobacter spp. (29/30); Klebsiella pneumoniae (62/72); Pseudomonas aeruginosa (124/125); and Serratia marcescens (18/21); respectively. From the 102 clinical samples, 104 bacterial species were identified with the BC-GN assay, whereas 110 were identified with the standard methods. The BC-GN assay also detected all β-lactam resistance genes tested (233 genes), including 54 bla(CTX-M), 119 bla(IMP), 8 bla(KPC), 16 bla(NDM), 24 bla(OXA-23), 1 bla(OXA-24/40), 1 bla(OXA-48), 4 bla(OXA-58), and 6 blaVIM. The data shows that the BC-GN assay provides rapid detection of GNB and β-lactam resistance genes in positive blood cultures and has the potential to contributing to optimal patient management by earlier detection of major antimicrobial resistance genes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0094064</identifier><identifier>PMID: 24705449</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acinetobacter ; Acinetobacter baumannii ; Amides ; Analysis ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial resistance ; Assaying ; Automation ; Bacteria ; Bacterial genetics ; Bacterial pneumonia ; Biology and Life Sciences ; Blood ; Blood culture ; Care and treatment ; Citrobacter ; Diagnosis ; Diagnostic systems ; Disease control ; Disease prevention ; Drug resistance ; Drug resistance in microorganisms ; Drug Resistance, Bacterial - genetics ; E coli ; Enterobacter cloacae ; Epidemiology ; Escherichia coli ; FDA approval ; Genes ; Genetic aspects ; Gram-negative bacteria ; Gram-Negative Bacteria - classification ; Gram-Negative Bacteria - drug effects ; Gram-Negative Bacteria - genetics ; Gram-Negative Bacteria - isolation & purification ; Gram-Negative Bacterial Infections - diagnosis ; Gram-Negative Bacterial Infections - microbiology ; Hospitals ; Humans ; Identification ; Identification methods ; Infectious diseases ; Klebsiella ; Klebsiella pneumoniae ; Laboratories ; Laboratory methods ; Mass spectrometry ; Medical diagnosis ; Medicine ; Medicine and Health Sciences ; Microbial Sensitivity Tests ; Molecular Diagnostic Techniques - methods ; Multiplexing ; Nanospheres ; Physiological aspects ; Pneumonia ; Pseudomonas aeruginosa ; Research and Analysis Methods ; Risk factors ; Scientific imaging ; Sepsis ; Staphylococcus aureus ; Staphylococcus infections ; Surveillance ; Womens health</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e94064-e94064</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Tojo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Tojo et al 2014 Tojo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-e2c78341bd9b7525d3285e11164573dc39ef0c41cd910fddd1bff28a81f99b7e3</citedby><cites>FETCH-LOGICAL-c692t-e2c78341bd9b7525d3285e11164573dc39ef0c41cd910fddd1bff28a81f99b7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976387/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976387/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23847,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24705449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Heimesaat, Markus M.</contributor><creatorcontrib>Tojo, Masayoshi</creatorcontrib><creatorcontrib>Fujita, Takahiro</creatorcontrib><creatorcontrib>Ainoda, Yusuke</creatorcontrib><creatorcontrib>Nagamatsu, Maki</creatorcontrib><creatorcontrib>Hayakawa, Kayoko</creatorcontrib><creatorcontrib>Mezaki, Kazuhisa</creatorcontrib><creatorcontrib>Sakurai, Aki</creatorcontrib><creatorcontrib>Masui, Yoshinori</creatorcontrib><creatorcontrib>Yazaki, Hirohisa</creatorcontrib><creatorcontrib>Takahashi, Hiroshi</creatorcontrib><creatorcontrib>Miyoshi-Akiyama, Tohru</creatorcontrib><creatorcontrib>Totsuka, Kyoichi</creatorcontrib><creatorcontrib>Kirikae, Teruo</creatorcontrib><creatorcontrib>Ohmagari, Norio</creatorcontrib><title>Evaluation of an automated rapid diagnostic assay for detection of Gram-negative bacteria and their drug-resistance genes in positive blood cultures</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We evaluated the performance of the Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN; Nanosphere, Northbrook, IL, USA), an automated multiplex assay for rapid identification of positive blood cultures caused by 9 Gram-negative bacteria (GNB) and for detection of 9 genes associated with β-lactam resistance. The BC-GN assay can be performed directly from positive blood cultures with 5 minutes of hands-on and 2 hours of run time per sample. A total of 397 GNB positive blood cultures were analyzed using the BC-GN assay. Of the 397 samples, 295 were simulated samples prepared by inoculating GNB into blood culture bottles, and the remaining were clinical samples from 102 patients with positive blood cultures. Aliquots of the positive blood cultures were tested by the BC-GN assay. The results of bacterial identification between the BC-GN assay and standard laboratory methods were as follows: Acinetobacter spp. (39 isolates for the BC-GN assay/39 for the standard methods), Citrobacter spp. (7/7), Escherichia coli (87/87), Klebsiella oxytoca (13/13), and Proteus spp. (11/11); Enterobacter spp. (29/30); Klebsiella pneumoniae (62/72); Pseudomonas aeruginosa (124/125); and Serratia marcescens (18/21); respectively. From the 102 clinical samples, 104 bacterial species were identified with the BC-GN assay, whereas 110 were identified with the standard methods. The BC-GN assay also detected all β-lactam resistance genes tested (233 genes), including 54 bla(CTX-M), 119 bla(IMP), 8 bla(KPC), 16 bla(NDM), 24 bla(OXA-23), 1 bla(OXA-24/40), 1 bla(OXA-48), 4 bla(OXA-58), and 6 blaVIM. The data shows that the BC-GN assay provides rapid detection of GNB and β-lactam resistance genes in positive blood cultures and has the potential to contributing to optimal patient management by earlier detection of major antimicrobial resistance genes.</description><subject>Acinetobacter</subject><subject>Acinetobacter baumannii</subject><subject>Amides</subject><subject>Analysis</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial resistance</subject><subject>Assaying</subject><subject>Automation</subject><subject>Bacteria</subject><subject>Bacterial genetics</subject><subject>Bacterial pneumonia</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood culture</subject><subject>Care and treatment</subject><subject>Citrobacter</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>Drug resistance</subject><subject>Drug resistance in microorganisms</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>E coli</subject><subject>Enterobacter cloacae</subject><subject>Epidemiology</subject><subject>Escherichia coli</subject><subject>FDA approval</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Gram-negative bacteria</subject><subject>Gram-Negative Bacteria - classification</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-Negative Bacteria - genetics</subject><subject>Gram-Negative Bacteria - isolation & purification</subject><subject>Gram-Negative Bacterial Infections - diagnosis</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Identification</subject><subject>Identification methods</subject><subject>Infectious diseases</subject><subject>Klebsiella</subject><subject>Klebsiella pneumoniae</subject><subject>Laboratories</subject><subject>Laboratory methods</subject><subject>Mass spectrometry</subject><subject>Medical diagnosis</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Multiplexing</subject><subject>Nanospheres</subject><subject>Physiological aspects</subject><subject>Pneumonia</subject><subject>Pseudomonas aeruginosa</subject><subject>Research and Analysis Methods</subject><subject>Risk factors</subject><subject>Scientific imaging</subject><subject>Sepsis</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Surveillance</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99qFDEUxgdRbK2-gWhAEL3YNZkkM5MboZRaC4WC_27DmeTMbJbZyZpkin0PH9isuy270gvJRULy-77knJxTFC8ZnTNesw9LP4URhvnajzinVAlaiUfFMVO8nFUl5Y_31kfFsxiXlEreVNXT4qgUNZVCqOPi9_kNDBMk50fiOwIjgSn5FSS0JMDaWWId9KOPyRkCMcIt6XwgFhOaO9FFgNVsxD673CBpwSQMDrKXJWmBLtNh6mcBo4sJRoOkxxEjcSNZ--i2osF7S8w0pClzz4snHQwRX-zmk-L7p_NvZ59nV9cXl2enVzNTqTLNsDR1wwVrrWprWUrLy0YiY6wSsubWcIUdNYIZqxjtrLWs7bqygYZ1KiuQnxSvt77rwUe9S2jUTNV1LRpaqUxcbgnrYanXwa0g3GoPTv_d8KHXEHJqBtSqtMBlC500VvDaQGWERGpF00qjGGSvj7vbpnaF1uCYAgwHpocno1vo3t9oruqKN3U2eLczCP7nhDHplYsGhwFG9FN-t2RCcCkZzeibf9CHo9tRPeQA3Nj5fK_ZmOpTXkvZ8FJtqPkDVB4WV87k6utc3j8QvD8QZCbhr9TDFKO-_Prl_9nrH4fs2z12gTCkRfTDtKnDeAiKLWiCjzFgd59kRvWmee6yoTfNo3fNk2Wv9j_oXnTXLfwPKckX9A</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Tojo, Masayoshi</creator><creator>Fujita, Takahiro</creator><creator>Ainoda, Yusuke</creator><creator>Nagamatsu, Maki</creator><creator>Hayakawa, Kayoko</creator><creator>Mezaki, Kazuhisa</creator><creator>Sakurai, Aki</creator><creator>Masui, Yoshinori</creator><creator>Yazaki, Hirohisa</creator><creator>Takahashi, Hiroshi</creator><creator>Miyoshi-Akiyama, Tohru</creator><creator>Totsuka, Kyoichi</creator><creator>Kirikae, Teruo</creator><creator>Ohmagari, Norio</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140401</creationdate><title>Evaluation of an automated rapid diagnostic assay for detection of Gram-negative bacteria and their drug-resistance genes in positive blood cultures</title><author>Tojo, Masayoshi ; Fujita, Takahiro ; Ainoda, Yusuke ; Nagamatsu, Maki ; Hayakawa, Kayoko ; Mezaki, Kazuhisa ; Sakurai, Aki ; Masui, Yoshinori ; Yazaki, Hirohisa ; Takahashi, Hiroshi ; Miyoshi-Akiyama, Tohru ; Totsuka, Kyoichi ; Kirikae, Teruo ; Ohmagari, Norio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-e2c78341bd9b7525d3285e11164573dc39ef0c41cd910fddd1bff28a81f99b7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acinetobacter</topic><topic>Acinetobacter baumannii</topic><topic>Amides</topic><topic>Analysis</topic><topic>Anti-Bacterial Agents - 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isolation & purification</topic><topic>Gram-Negative Bacterial Infections - diagnosis</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Identification</topic><topic>Identification methods</topic><topic>Infectious diseases</topic><topic>Klebsiella</topic><topic>Klebsiella pneumoniae</topic><topic>Laboratories</topic><topic>Laboratory methods</topic><topic>Mass spectrometry</topic><topic>Medical diagnosis</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Multiplexing</topic><topic>Nanospheres</topic><topic>Physiological aspects</topic><topic>Pneumonia</topic><topic>Pseudomonas aeruginosa</topic><topic>Research and Analysis Methods</topic><topic>Risk factors</topic><topic>Scientific imaging</topic><topic>Sepsis</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><topic>Surveillance</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tojo, Masayoshi</creatorcontrib><creatorcontrib>Fujita, Takahiro</creatorcontrib><creatorcontrib>Ainoda, Yusuke</creatorcontrib><creatorcontrib>Nagamatsu, Maki</creatorcontrib><creatorcontrib>Hayakawa, Kayoko</creatorcontrib><creatorcontrib>Mezaki, Kazuhisa</creatorcontrib><creatorcontrib>Sakurai, Aki</creatorcontrib><creatorcontrib>Masui, Yoshinori</creatorcontrib><creatorcontrib>Yazaki, Hirohisa</creatorcontrib><creatorcontrib>Takahashi, Hiroshi</creatorcontrib><creatorcontrib>Miyoshi-Akiyama, Tohru</creatorcontrib><creatorcontrib>Totsuka, Kyoichi</creatorcontrib><creatorcontrib>Kirikae, Teruo</creatorcontrib><creatorcontrib>Ohmagari, Norio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tojo, Masayoshi</au><au>Fujita, Takahiro</au><au>Ainoda, Yusuke</au><au>Nagamatsu, Maki</au><au>Hayakawa, Kayoko</au><au>Mezaki, Kazuhisa</au><au>Sakurai, Aki</au><au>Masui, Yoshinori</au><au>Yazaki, Hirohisa</au><au>Takahashi, Hiroshi</au><au>Miyoshi-Akiyama, Tohru</au><au>Totsuka, Kyoichi</au><au>Kirikae, Teruo</au><au>Ohmagari, Norio</au><au>Heimesaat, Markus M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of an automated rapid diagnostic assay for detection of Gram-negative bacteria and their drug-resistance genes in positive blood cultures</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e94064</spage><epage>e94064</epage><pages>e94064-e94064</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>We evaluated the performance of the Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN; Nanosphere, Northbrook, IL, USA), an automated multiplex assay for rapid identification of positive blood cultures caused by 9 Gram-negative bacteria (GNB) and for detection of 9 genes associated with β-lactam resistance. The BC-GN assay can be performed directly from positive blood cultures with 5 minutes of hands-on and 2 hours of run time per sample. A total of 397 GNB positive blood cultures were analyzed using the BC-GN assay. Of the 397 samples, 295 were simulated samples prepared by inoculating GNB into blood culture bottles, and the remaining were clinical samples from 102 patients with positive blood cultures. Aliquots of the positive blood cultures were tested by the BC-GN assay. The results of bacterial identification between the BC-GN assay and standard laboratory methods were as follows: Acinetobacter spp. (39 isolates for the BC-GN assay/39 for the standard methods), Citrobacter spp. (7/7), Escherichia coli (87/87), Klebsiella oxytoca (13/13), and Proteus spp. (11/11); Enterobacter spp. (29/30); Klebsiella pneumoniae (62/72); Pseudomonas aeruginosa (124/125); and Serratia marcescens (18/21); respectively. From the 102 clinical samples, 104 bacterial species were identified with the BC-GN assay, whereas 110 were identified with the standard methods. The BC-GN assay also detected all β-lactam resistance genes tested (233 genes), including 54 bla(CTX-M), 119 bla(IMP), 8 bla(KPC), 16 bla(NDM), 24 bla(OXA-23), 1 bla(OXA-24/40), 1 bla(OXA-48), 4 bla(OXA-58), and 6 blaVIM. The data shows that the BC-GN assay provides rapid detection of GNB and β-lactam resistance genes in positive blood cultures and has the potential to contributing to optimal patient management by earlier detection of major antimicrobial resistance genes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24705449</pmid><doi>10.1371/journal.pone.0094064</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-04, Vol.9 (4), p.e94064-e94064 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1977748069 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Acinetobacter Acinetobacter baumannii Amides Analysis Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial resistance Assaying Automation Bacteria Bacterial genetics Bacterial pneumonia Biology and Life Sciences Blood Blood culture Care and treatment Citrobacter Diagnosis Diagnostic systems Disease control Disease prevention Drug resistance Drug resistance in microorganisms Drug Resistance, Bacterial - genetics E coli Enterobacter cloacae Epidemiology Escherichia coli FDA approval Genes Genetic aspects Gram-negative bacteria Gram-Negative Bacteria - classification Gram-Negative Bacteria - drug effects Gram-Negative Bacteria - genetics Gram-Negative Bacteria - isolation & purification Gram-Negative Bacterial Infections - diagnosis Gram-Negative Bacterial Infections - microbiology Hospitals Humans Identification Identification methods Infectious diseases Klebsiella Klebsiella pneumoniae Laboratories Laboratory methods Mass spectrometry Medical diagnosis Medicine Medicine and Health Sciences Microbial Sensitivity Tests Molecular Diagnostic Techniques - methods Multiplexing Nanospheres Physiological aspects Pneumonia Pseudomonas aeruginosa Research and Analysis Methods Risk factors Scientific imaging Sepsis Staphylococcus aureus Staphylococcus infections Surveillance Womens health |
| title | Evaluation of an automated rapid diagnostic assay for detection of Gram-negative bacteria and their drug-resistance genes in positive blood cultures |
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