The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids
RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-02, Vol.9 (2), p.e89628-e89628 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e89628 |
|---|---|
| container_issue | 2 |
| container_start_page | e89628 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Qiu, Yang Wang, Zhaowei Liu, Yongxiang Han, Yajuan Miao, Meng Qi, Nan Yang, Jie Xia, Hongjie Li, Xiaofeng Qin, Cheng-Feng Hu, Yuanyang Zhou, Xi |
| description | RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of mitochondrial membrane lipids (MMLs) on WhNV protein A activity in vitro. Our investigations revealed the self-interaction of WhNV protein A is accomplished via two different patterns (i.e., homotypic and heterotypic self-interactions via different interfaces). MMLs stimulated the protein A self-interaction, and this stimulation exhibited selectivity for specific phospholipids. Moreover, we found that specific phospholipids differently favor the two self-interaction patterns. Furthermore, manipulating specific phospholipid metabolism affected protein A self-interaction and the activity of protein A to replicate RNA in cells. Taken together, our findings reveal the direct effects of membrane lipids on a nodaviral RNA replicase. |
| doi_str_mv | 10.1371/journal.pone.0089628 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1977558238</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478792739</galeid><doaj_id>oai_doaj_org_article_6e1ee54e0c5c45ef823173106c5f2041</doaj_id><sourcerecordid>A478792739</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-60fd1f7cf5b6d9cf3215ecb2a7050b9767b79e37a87084bbc1872b9930f51993</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlomvZGWJZVBxYWdPDCm5CmJ9MMaTIm7eL--8043WUqeyENJCTPeU_y9pwse43RElOOP239GJy0y513sESoqktSPclOcU3JoiSIPj1an2QvYtwixGhVls-zE1KwqqwJPs1-rTvII1i9MG6AINVgvMu9zmXufCtvTBhjHmBnjZIRchNzcJ10Ctq8uc17M3jVedcGI23eQ98E6SC3Zmfa-DJ7pqWN8Gqaz7L1l8v1xbfF1fXX1cX51UKlKwyLEukWa640a8q2VpoSzEA1RHLEUFPzkje8BsplxVFVNI3CFSdNXVOkGU7TWfb2ILuzPorJlShwzTljFaFVIlYHovVyK3bB9DLcCi-N-Lvhw0bIMBhlQZSAAVgBSDFVMNApHnOKUamYJqjASevzlG1semgVuCFIOxOdnzjTiY2_EbSmRRpJ4MMkEPzvEeIgehMVWJuM82O6N0tpGMIFSei7f9DHXzdRG5keYJz2Ka_ai4rzgle8JpzuXVo-QqWvhd6oVELapP1ZwMdZQGIG-DNs5BijWP34_v_s9c85-_6I7UDaoYvejvu6i3OwOIAq-BgD6AeTMRL7Drh3Q-w7QEwdkMLeHP-gh6D7kqd38cEAfg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1977558238</pqid></control><display><type>article</type><title>The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Qiu, Yang ; Wang, Zhaowei ; Liu, Yongxiang ; Han, Yajuan ; Miao, Meng ; Qi, Nan ; Yang, Jie ; Xia, Hongjie ; Li, Xiaofeng ; Qin, Cheng-Feng ; Hu, Yuanyang ; Zhou, Xi</creator><creatorcontrib>Qiu, Yang ; Wang, Zhaowei ; Liu, Yongxiang ; Han, Yajuan ; Miao, Meng ; Qi, Nan ; Yang, Jie ; Xia, Hongjie ; Li, Xiaofeng ; Qin, Cheng-Feng ; Hu, Yuanyang ; Zhou, Xi</creatorcontrib><description>RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of mitochondrial membrane lipids (MMLs) on WhNV protein A activity in vitro. Our investigations revealed the self-interaction of WhNV protein A is accomplished via two different patterns (i.e., homotypic and heterotypic self-interactions via different interfaces). MMLs stimulated the protein A self-interaction, and this stimulation exhibited selectivity for specific phospholipids. Moreover, we found that specific phospholipids differently favor the two self-interaction patterns. Furthermore, manipulating specific phospholipid metabolism affected protein A self-interaction and the activity of protein A to replicate RNA in cells. Taken together, our findings reveal the direct effects of membrane lipids on a nodaviral RNA replicase.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0089628</identifier><identifier>PMID: 24586921</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology ; Biosynthesis ; Drosophila ; Epidemiology ; Hepatitis ; Insects ; Interfaces ; Laboratories ; Life sciences ; Lipid metabolism ; Lipids ; Membrane Lipids - metabolism ; Membranes ; Metabolism ; Mitochondria ; Mitochondrial Membranes - metabolism ; Mutation ; Nodaviridae - physiology ; Pathogens ; Phospholipids ; Phospholipids - physiology ; Physiological aspects ; Plasmids ; Protein A ; Protein interaction ; Protein turnover ; Protein-protein interactions ; Proteins ; Replicase ; Replication ; Ribonucleic acid ; RNA ; RNA polymerase ; RNA Replicase - metabolism ; RNA viruses ; RNA-directed RNA polymerase ; Selectivity ; Viral Proteins - metabolism ; Virology ; Virus replication ; Virus Replication - physiology ; Viruses</subject><ispartof>PloS one, 2014-02, Vol.9 (2), p.e89628-e89628</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Qiu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Qiu et al 2014 Qiu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-60fd1f7cf5b6d9cf3215ecb2a7050b9767b79e37a87084bbc1872b9930f51993</citedby><cites>FETCH-LOGICAL-c692t-60fd1f7cf5b6d9cf3215ecb2a7050b9767b79e37a87084bbc1872b9930f51993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934934/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934934/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24586921$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Yang</creatorcontrib><creatorcontrib>Wang, Zhaowei</creatorcontrib><creatorcontrib>Liu, Yongxiang</creatorcontrib><creatorcontrib>Han, Yajuan</creatorcontrib><creatorcontrib>Miao, Meng</creatorcontrib><creatorcontrib>Qi, Nan</creatorcontrib><creatorcontrib>Yang, Jie</creatorcontrib><creatorcontrib>Xia, Hongjie</creatorcontrib><creatorcontrib>Li, Xiaofeng</creatorcontrib><creatorcontrib>Qin, Cheng-Feng</creatorcontrib><creatorcontrib>Hu, Yuanyang</creatorcontrib><creatorcontrib>Zhou, Xi</creatorcontrib><title>The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of mitochondrial membrane lipids (MMLs) on WhNV protein A activity in vitro. Our investigations revealed the self-interaction of WhNV protein A is accomplished via two different patterns (i.e., homotypic and heterotypic self-interactions via different interfaces). MMLs stimulated the protein A self-interaction, and this stimulation exhibited selectivity for specific phospholipids. Moreover, we found that specific phospholipids differently favor the two self-interaction patterns. Furthermore, manipulating specific phospholipid metabolism affected protein A self-interaction and the activity of protein A to replicate RNA in cells. Taken together, our findings reveal the direct effects of membrane lipids on a nodaviral RNA replicase.</description><subject>Biology</subject><subject>Biosynthesis</subject><subject>Drosophila</subject><subject>Epidemiology</subject><subject>Hepatitis</subject><subject>Insects</subject><subject>Interfaces</subject><subject>Laboratories</subject><subject>Life sciences</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Membrane Lipids - metabolism</subject><subject>Membranes</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>Mitochondrial Membranes - metabolism</subject><subject>Mutation</subject><subject>Nodaviridae - physiology</subject><subject>Pathogens</subject><subject>Phospholipids</subject><subject>Phospholipids - physiology</subject><subject>Physiological aspects</subject><subject>Plasmids</subject><subject>Protein A</subject><subject>Protein interaction</subject><subject>Protein turnover</subject><subject>Protein-protein interactions</subject><subject>Proteins</subject><subject>Replicase</subject><subject>Replication</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA Replicase - metabolism</subject><subject>RNA viruses</subject><subject>RNA-directed RNA polymerase</subject><subject>Selectivity</subject><subject>Viral Proteins - metabolism</subject><subject>Virology</subject><subject>Virus replication</subject><subject>Virus Replication - physiology</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlomvZGWJZVBxYWdPDCm5CmJ9MMaTIm7eL--8043WUqeyENJCTPeU_y9pwse43RElOOP239GJy0y513sESoqktSPclOcU3JoiSIPj1an2QvYtwixGhVls-zE1KwqqwJPs1-rTvII1i9MG6AINVgvMu9zmXufCtvTBhjHmBnjZIRchNzcJ10Ctq8uc17M3jVedcGI23eQ98E6SC3Zmfa-DJ7pqWN8Gqaz7L1l8v1xbfF1fXX1cX51UKlKwyLEukWa640a8q2VpoSzEA1RHLEUFPzkje8BsplxVFVNI3CFSdNXVOkGU7TWfb2ILuzPorJlShwzTljFaFVIlYHovVyK3bB9DLcCi-N-Lvhw0bIMBhlQZSAAVgBSDFVMNApHnOKUamYJqjASevzlG1semgVuCFIOxOdnzjTiY2_EbSmRRpJ4MMkEPzvEeIgehMVWJuM82O6N0tpGMIFSei7f9DHXzdRG5keYJz2Ka_ai4rzgle8JpzuXVo-QqWvhd6oVELapP1ZwMdZQGIG-DNs5BijWP34_v_s9c85-_6I7UDaoYvejvu6i3OwOIAq-BgD6AeTMRL7Drh3Q-w7QEwdkMLeHP-gh6D7kqd38cEAfg</recordid><startdate>20140225</startdate><enddate>20140225</enddate><creator>Qiu, Yang</creator><creator>Wang, Zhaowei</creator><creator>Liu, Yongxiang</creator><creator>Han, Yajuan</creator><creator>Miao, Meng</creator><creator>Qi, Nan</creator><creator>Yang, Jie</creator><creator>Xia, Hongjie</creator><creator>Li, Xiaofeng</creator><creator>Qin, Cheng-Feng</creator><creator>Hu, Yuanyang</creator><creator>Zhou, Xi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140225</creationdate><title>The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids</title><author>Qiu, Yang ; Wang, Zhaowei ; Liu, Yongxiang ; Han, Yajuan ; Miao, Meng ; Qi, Nan ; Yang, Jie ; Xia, Hongjie ; Li, Xiaofeng ; Qin, Cheng-Feng ; Hu, Yuanyang ; Zhou, Xi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-60fd1f7cf5b6d9cf3215ecb2a7050b9767b79e37a87084bbc1872b9930f51993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biology</topic><topic>Biosynthesis</topic><topic>Drosophila</topic><topic>Epidemiology</topic><topic>Hepatitis</topic><topic>Insects</topic><topic>Interfaces</topic><topic>Laboratories</topic><topic>Life sciences</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Membrane Lipids - metabolism</topic><topic>Membranes</topic><topic>Metabolism</topic><topic>Mitochondria</topic><topic>Mitochondrial Membranes - metabolism</topic><topic>Mutation</topic><topic>Nodaviridae - physiology</topic><topic>Pathogens</topic><topic>Phospholipids</topic><topic>Phospholipids - physiology</topic><topic>Physiological aspects</topic><topic>Plasmids</topic><topic>Protein A</topic><topic>Protein interaction</topic><topic>Protein turnover</topic><topic>Protein-protein interactions</topic><topic>Proteins</topic><topic>Replicase</topic><topic>Replication</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA polymerase</topic><topic>RNA Replicase - metabolism</topic><topic>RNA viruses</topic><topic>RNA-directed RNA polymerase</topic><topic>Selectivity</topic><topic>Viral Proteins - metabolism</topic><topic>Virology</topic><topic>Virus replication</topic><topic>Virus Replication - physiology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Yang</creatorcontrib><creatorcontrib>Wang, Zhaowei</creatorcontrib><creatorcontrib>Liu, Yongxiang</creatorcontrib><creatorcontrib>Han, Yajuan</creatorcontrib><creatorcontrib>Miao, Meng</creatorcontrib><creatorcontrib>Qi, Nan</creatorcontrib><creatorcontrib>Yang, Jie</creatorcontrib><creatorcontrib>Xia, Hongjie</creatorcontrib><creatorcontrib>Li, Xiaofeng</creatorcontrib><creatorcontrib>Qin, Cheng-Feng</creatorcontrib><creatorcontrib>Hu, Yuanyang</creatorcontrib><creatorcontrib>Zhou, Xi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Yang</au><au>Wang, Zhaowei</au><au>Liu, Yongxiang</au><au>Han, Yajuan</au><au>Miao, Meng</au><au>Qi, Nan</au><au>Yang, Jie</au><au>Xia, Hongjie</au><au>Li, Xiaofeng</au><au>Qin, Cheng-Feng</au><au>Hu, Yuanyang</au><au>Zhou, Xi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-02-25</date><risdate>2014</risdate><volume>9</volume><issue>2</issue><spage>e89628</spage><epage>e89628</epage><pages>e89628-e89628</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of mitochondrial membrane lipids (MMLs) on WhNV protein A activity in vitro. Our investigations revealed the self-interaction of WhNV protein A is accomplished via two different patterns (i.e., homotypic and heterotypic self-interactions via different interfaces). MMLs stimulated the protein A self-interaction, and this stimulation exhibited selectivity for specific phospholipids. Moreover, we found that specific phospholipids differently favor the two self-interaction patterns. Furthermore, manipulating specific phospholipid metabolism affected protein A self-interaction and the activity of protein A to replicate RNA in cells. Taken together, our findings reveal the direct effects of membrane lipids on a nodaviral RNA replicase.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24586921</pmid><doi>10.1371/journal.pone.0089628</doi><tpages>e89628</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-02, Vol.9 (2), p.e89628-e89628 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1977558238 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Biology Biosynthesis Drosophila Epidemiology Hepatitis Insects Interfaces Laboratories Life sciences Lipid metabolism Lipids Membrane Lipids - metabolism Membranes Metabolism Mitochondria Mitochondrial Membranes - metabolism Mutation Nodaviridae - physiology Pathogens Phospholipids Phospholipids - physiology Physiological aspects Plasmids Protein A Protein interaction Protein turnover Protein-protein interactions Proteins Replicase Replication Ribonucleic acid RNA RNA polymerase RNA Replicase - metabolism RNA viruses RNA-directed RNA polymerase Selectivity Viral Proteins - metabolism Virology Virus replication Virus Replication - physiology Viruses |
| title | The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T11%3A47%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20self-interaction%20of%20a%20nodavirus%20replicase%20is%20enhanced%20by%20mitochondrial%20membrane%20lipids&rft.jtitle=PloS%20one&rft.au=Qiu,%20Yang&rft.date=2014-02-25&rft.volume=9&rft.issue=2&rft.spage=e89628&rft.epage=e89628&rft.pages=e89628-e89628&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0089628&rft_dat=%3Cgale_plos_%3EA478792739%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1977558238&rft_id=info:pmid/24586921&rft_galeid=A478792739&rft_doaj_id=oai_doaj_org_article_6e1ee54e0c5c45ef823173106c5f2041&rfr_iscdi=true |