Identification of the maize gravitropism gene lazy plant1 by a transposon-tagging genome resequencing strategy

Since their initial discovery, transposons have been widely used as mutagens for forward and reverse genetic screens in a range of organisms. The problems of high copy number and sequence divergence among related transposons have often limited the efficiency at which tagged genes can be identified....

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Veröffentlicht in:	PloS one 2014-01, Vol.9 (1), p.e87053
Hauptverfasser:	Howard, 3rd, Thomas P, Hayward, Andrew P, Tordillos, Anthony, Fragoso, Christopher, Moreno, Maria A, Tohme, Joe, Kausch, Albert P, Mottinger, John P, Dellaporta, Stephen L
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Base Sequence Biology Cell Nucleus - genetics Cell Nucleus - metabolism Cloning Conserved sequence Copy number Corn Deoxyribonucleic acid Developmental biology Divergence DNA DNA methylation DNA Restriction Enzymes - metabolism DNA sequencing DNA Transposable Elements - genetics DNA, Plant - chemistry DNA, Plant - genetics DNA, Plant - metabolism Enzymes Fluorescence Fluorescence microscopy Gene sequencing Genes Genes, Plant - genetics Genetic research Genetic screening Genetic testing Genetics Genome, Plant - genetics Genomes Genomic Library Genomics Gravitation Gravitropism Gravitropism - genetics Gravity Identification Insertion Membrane proteins Membrane Proteins - genetics Membrane Proteins - metabolism Microscopy, Fluorescence Molecular biology Molecular Sequence Data Mutagenesis Mutagenesis, Insertional Mutagens Mutation Plant Proteins - genetics Plant Proteins - metabolism Plant Shoots - genetics Plant Shoots - physiology Plants (botany) Polymerase Chain Reaction Sequence Analysis, DNA - methods Sequence Homology, Nucleic Acid Shoots Transposons Zea mays Zea mays - genetics Zea mays - physiology
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