Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water
The ability of euryhaline Mozambique tilapia to tolerate extreme environmental salinities makes it an excellent model for investigating iono-regulation. This study aimed to characterize and fill important information gap of the expression levels of key ion transporters for Na(+) and Cl(-) in the gil...
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| description | The ability of euryhaline Mozambique tilapia to tolerate extreme environmental salinities makes it an excellent model for investigating iono-regulation. This study aimed to characterize and fill important information gap of the expression levels of key ion transporters for Na(+) and Cl(-) in the gill and esophageal-gastrointestinal tract of Mozambique tilapia acclimated to freshwater (0 ppt), seawater (30 ppt) and hypersaline (70 ppt) environments. Among the seven genes studied, it was found that nkcc2, nkcc1a, cftr, nka-α1 and nka-α3, were more responsive to salinity challenge than nkcc1b and ncc within the investigated tissues. The ncc expression was restricted to gills of freshwater-acclimated fish while nkcc2 expression was restricted to intestinal segments irrespective of salinity challenge. Among the tissues investigated, gill and posterior intestine were found to be highly responsive to salinity changes, followed by anterior and middle intestine. Both esophagus and stomach displayed significant up-regulation of nka-α1 and nka-α3, but not nkcc isoforms and cftr, in hypersaline-acclimated fish suggesting a response to hypersalinity challenge and involvement of other forms of transporters in iono-regulation. Changes in gene expression levels were partly corroborated by immunohistochemical localization of transport proteins. Apical expression of Ncc was found in Nka-immunoreactive cells in freshwater-acclimated gills while Nkcc co-localized with Nka-immunoreactive cells expressing Cftr apically in seawater- and hypersaline-acclimated gills. In the intestine, Nkcc-stained apical brush border was found in Nka-immunoreactive cells at greater levels under hypersaline conditions. These findings provided new insights into the responsiveness of these genes and tissues under hypersalinity challenge, specifically the posterior intestine being vital for salt absorption and iono-osmoregulation in the Mozambique tilapia; its ability to survive in hypersalinity may be in part related to its ability to up-regulate key ion transporters in the posterior intestine. The findings pave the way for future iono-regulatory studies on the Mozambique tilapia esophageal-gastrointestinal tract. |
| doi_str_mv | 10.1371/journal.pone.0087591 |
| format | Article |
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This study aimed to characterize and fill important information gap of the expression levels of key ion transporters for Na(+) and Cl(-) in the gill and esophageal-gastrointestinal tract of Mozambique tilapia acclimated to freshwater (0 ppt), seawater (30 ppt) and hypersaline (70 ppt) environments. Among the seven genes studied, it was found that nkcc2, nkcc1a, cftr, nka-α1 and nka-α3, were more responsive to salinity challenge than nkcc1b and ncc within the investigated tissues. The ncc expression was restricted to gills of freshwater-acclimated fish while nkcc2 expression was restricted to intestinal segments irrespective of salinity challenge. Among the tissues investigated, gill and posterior intestine were found to be highly responsive to salinity changes, followed by anterior and middle intestine. Both esophagus and stomach displayed significant up-regulation of nka-α1 and nka-α3, but not nkcc isoforms and cftr, in hypersaline-acclimated fish suggesting a response to hypersalinity challenge and involvement of other forms of transporters in iono-regulation. Changes in gene expression levels were partly corroborated by immunohistochemical localization of transport proteins. Apical expression of Ncc was found in Nka-immunoreactive cells in freshwater-acclimated gills while Nkcc co-localized with Nka-immunoreactive cells expressing Cftr apically in seawater- and hypersaline-acclimated gills. In the intestine, Nkcc-stained apical brush border was found in Nka-immunoreactive cells at greater levels under hypersaline conditions. These findings provided new insights into the responsiveness of these genes and tissues under hypersalinity challenge, specifically the posterior intestine being vital for salt absorption and iono-osmoregulation in the Mozambique tilapia; its ability to survive in hypersalinity may be in part related to its ability to up-regulate key ion transporters in the posterior intestine. The findings pave the way for future iono-regulatory studies on the Mozambique tilapia esophageal-gastrointestinal tract.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0087591</identifier><identifier>PMID: 24498146</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acclimatization - physiology ; Anguilla japonica ; Animal tissues ; Animals ; Antigens ; Biology ; Esophagus ; Esophagus - metabolism ; Fish ; Fish Proteins - biosynthesis ; Fish Proteins - genetics ; Fishes ; Fresh Water ; Freshwater ; Freshwater environments ; Freshwater fish ; Gastrointestinal system ; Gastrointestinal tract ; Gene expression ; Gene Expression Regulation - physiology ; Genes ; Gills ; Intestine ; Ion Channels - biosynthesis ; Ion Channels - genetics ; Ion Transport - physiology ; Isoforms ; Localization ; Oreochromis mossambicus ; Osmoregulation ; Physiology ; Proteins ; Rodents ; Salinity ; Salinity effects ; Salts ; Seawater ; Stomach ; Studies ; Tilapia ; Tilapia - physiology</subject><ispartof>PloS one, 2014-01, Vol.9 (1), p.e87591</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Li et al 2014 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ecec8a7b86a154246ba476f3b2dbfd13a04c2cde7dd3ebd478d8de286477e0d03</citedby><cites>FETCH-LOGICAL-c692t-ecec8a7b86a154246ba476f3b2dbfd13a04c2cde7dd3ebd478d8de286477e0d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909219/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909219/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24498146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Planas, Josep V.</contributor><creatorcontrib>Li, Zhengjun</creatorcontrib><creatorcontrib>Lui, Eei Yin</creatorcontrib><creatorcontrib>Wilson, Jonathan M</creatorcontrib><creatorcontrib>Ip, Yuen Kwong</creatorcontrib><creatorcontrib>Lin, Qingsong</creatorcontrib><creatorcontrib>Lam, Toong Jin</creatorcontrib><creatorcontrib>Lam, Siew Hong</creatorcontrib><title>Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The ability of euryhaline Mozambique tilapia to tolerate extreme environmental salinities makes it an excellent model for investigating iono-regulation. This study aimed to characterize and fill important information gap of the expression levels of key ion transporters for Na(+) and Cl(-) in the gill and esophageal-gastrointestinal tract of Mozambique tilapia acclimated to freshwater (0 ppt), seawater (30 ppt) and hypersaline (70 ppt) environments. Among the seven genes studied, it was found that nkcc2, nkcc1a, cftr, nka-α1 and nka-α3, were more responsive to salinity challenge than nkcc1b and ncc within the investigated tissues. The ncc expression was restricted to gills of freshwater-acclimated fish while nkcc2 expression was restricted to intestinal segments irrespective of salinity challenge. Among the tissues investigated, gill and posterior intestine were found to be highly responsive to salinity changes, followed by anterior and middle intestine. Both esophagus and stomach displayed significant up-regulation of nka-α1 and nka-α3, but not nkcc isoforms and cftr, in hypersaline-acclimated fish suggesting a response to hypersalinity challenge and involvement of other forms of transporters in iono-regulation. Changes in gene expression levels were partly corroborated by immunohistochemical localization of transport proteins. Apical expression of Ncc was found in Nka-immunoreactive cells in freshwater-acclimated gills while Nkcc co-localized with Nka-immunoreactive cells expressing Cftr apically in seawater- and hypersaline-acclimated gills. In the intestine, Nkcc-stained apical brush border was found in Nka-immunoreactive cells at greater levels under hypersaline conditions. These findings provided new insights into the responsiveness of these genes and tissues under hypersalinity challenge, specifically the posterior intestine being vital for salt absorption and iono-osmoregulation in the Mozambique tilapia; its ability to survive in hypersalinity may be in part related to its ability to up-regulate key ion transporters in the posterior intestine. The findings pave the way for future iono-regulatory studies on the Mozambique tilapia esophageal-gastrointestinal tract.</description><subject>Acclimatization - physiology</subject><subject>Anguilla japonica</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Antigens</subject><subject>Biology</subject><subject>Esophagus</subject><subject>Esophagus - metabolism</subject><subject>Fish</subject><subject>Fish Proteins - biosynthesis</subject><subject>Fish Proteins - genetics</subject><subject>Fishes</subject><subject>Fresh Water</subject><subject>Freshwater</subject><subject>Freshwater environments</subject><subject>Freshwater fish</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genes</subject><subject>Gills</subject><subject>Intestine</subject><subject>Ion Channels - biosynthesis</subject><subject>Ion Channels - genetics</subject><subject>Ion Transport - physiology</subject><subject>Isoforms</subject><subject>Localization</subject><subject>Oreochromis mossambicus</subject><subject>Osmoregulation</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Salinity</subject><subject>Salinity effects</subject><subject>Salts</subject><subject>Seawater</subject><subject>Stomach</subject><subject>Studies</subject><subject>Tilapia</subject><subject>Tilapia - physiology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNU12L1DAULaK46-o_EA0IguCMSZNJ2xdhWVYdWBnw6zWkyW2bsdN0k1R3_IP-LdOZ7jIDCtKHJjfnnpyce2-SPCV4TmhG3qzt4DrZznvbwRzjPFsU5F5ySgqazniK6f2D9UnyyPs1xguac_4wOUkZK3LC-Gny-_Kmd-C9sR2yFfoOWzQug5Od760L4Dwycd8Aqk3bItlpBN72jaxBtrNa-uCs6QL4YKKaMVGFkQkGt21kazpAH-0vuSnN9QAomFb2RqKVA6saZzfGo431fjxXg0dSqdZsZACNgkVVVNagn3HrXiMPcrfaSWi2fVS2p99FHycPKtl6eDL9z5Kv7y6_XHyYXa3eLy_Or2aKF2mYgQKVy6zMuSQLljJeSpbxipapLitNqMRMpUpDpjWFUrMs17mGNOcsywBrTM-S53vevrVeTDXwghRZxlhasCIilnuEtnItehef47bCSiN2AetqIV0wqgXBU55Fcqx0yVgVi1OparHgCtOqIJTyyPV2um0oN6AVdNHf9oj0-KQzjajtD0ELXKRkFPNiInA2-u_DPyRPqFpGVaar7FjGWBslzqMFOU0JGZ8-_wsqfho2RsUurEyMHyW8OkqImAA3oZaD92L5-dP_Y1ffjrEvD7BN7MPQeNsOIXauPwayPVC52GMOqjvnCBbjEN26IcYhEtMQxbRnh67fJd1ODf0D9sMeZg</recordid><startdate>20140131</startdate><enddate>20140131</enddate><creator>Li, Zhengjun</creator><creator>Lui, Eei Yin</creator><creator>Wilson, Jonathan M</creator><creator>Ip, Yuen Kwong</creator><creator>Lin, Qingsong</creator><creator>Lam, Toong Jin</creator><creator>Lam, Siew Hong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140131</creationdate><title>Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water</title><author>Li, Zhengjun ; Lui, Eei Yin ; Wilson, Jonathan M ; Ip, Yuen Kwong ; Lin, Qingsong ; Lam, Toong Jin ; Lam, Siew Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-ecec8a7b86a154246ba476f3b2dbfd13a04c2cde7dd3ebd478d8de286477e0d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acclimatization - 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This study aimed to characterize and fill important information gap of the expression levels of key ion transporters for Na(+) and Cl(-) in the gill and esophageal-gastrointestinal tract of Mozambique tilapia acclimated to freshwater (0 ppt), seawater (30 ppt) and hypersaline (70 ppt) environments. Among the seven genes studied, it was found that nkcc2, nkcc1a, cftr, nka-α1 and nka-α3, were more responsive to salinity challenge than nkcc1b and ncc within the investigated tissues. The ncc expression was restricted to gills of freshwater-acclimated fish while nkcc2 expression was restricted to intestinal segments irrespective of salinity challenge. Among the tissues investigated, gill and posterior intestine were found to be highly responsive to salinity changes, followed by anterior and middle intestine. Both esophagus and stomach displayed significant up-regulation of nka-α1 and nka-α3, but not nkcc isoforms and cftr, in hypersaline-acclimated fish suggesting a response to hypersalinity challenge and involvement of other forms of transporters in iono-regulation. Changes in gene expression levels were partly corroborated by immunohistochemical localization of transport proteins. Apical expression of Ncc was found in Nka-immunoreactive cells in freshwater-acclimated gills while Nkcc co-localized with Nka-immunoreactive cells expressing Cftr apically in seawater- and hypersaline-acclimated gills. In the intestine, Nkcc-stained apical brush border was found in Nka-immunoreactive cells at greater levels under hypersaline conditions. These findings provided new insights into the responsiveness of these genes and tissues under hypersalinity challenge, specifically the posterior intestine being vital for salt absorption and iono-osmoregulation in the Mozambique tilapia; its ability to survive in hypersalinity may be in part related to its ability to up-regulate key ion transporters in the posterior intestine. The findings pave the way for future iono-regulatory studies on the Mozambique tilapia esophageal-gastrointestinal tract.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24498146</pmid><doi>10.1371/journal.pone.0087591</doi><tpages>e87591</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1977442949 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acclimatization - physiology Anguilla japonica Animal tissues Animals Antigens Biology Esophagus Esophagus - metabolism Fish Fish Proteins - biosynthesis Fish Proteins - genetics Fishes Fresh Water Freshwater Freshwater environments Freshwater fish Gastrointestinal system Gastrointestinal tract Gene expression Gene Expression Regulation - physiology Genes Gills Intestine Ion Channels - biosynthesis Ion Channels - genetics Ion Transport - physiology Isoforms Localization Oreochromis mossambicus Osmoregulation Physiology Proteins Rodents Salinity Salinity effects Salts Seawater Stomach Studies Tilapia Tilapia - physiology |
| title | Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T22%3A51%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20key%20ion%20transporters%20in%20the%20gill%20and%20esophageal-gastrointestinal%20tract%20of%20euryhaline%20Mozambique%20tilapia%20Oreochromis%20mossambicus%20acclimated%20to%20fresh%20water,%20seawater%20and%20hypersaline%20water&rft.jtitle=PloS%20one&rft.au=Li,%20Zhengjun&rft.date=2014-01-31&rft.volume=9&rft.issue=1&rft.spage=e87591&rft.pages=e87591-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0087591&rft_dat=%3Cgale_plos_%3EA478832110%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1977442949&rft_id=info:pmid/24498146&rft_galeid=A478832110&rft_doaj_id=oai_doaj_org_article_6267e280cdb44f538fcf556c03f91336&rfr_iscdi=true |