Stability evaluation of reference genes for gene expression analysis by RT-qPCR in soybean under different conditions

Real-time quantitative reverse transcription PCR is a sensitive and widely used technique to quantify gene expression. To achieve a reliable result, appropriate reference genes are highly required for normalization of transcripts in different samples. In this study, 9 previously published reference...

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Veröffentlicht in:	PloS one 2017-12, Vol.12 (12), p.e0189405-e0189405
Hauptverfasser:	Wan, Qiao, Chen, Shuilian, Shan, Zhihui, Yang, Zhonglu, Chen, Limiao, Zhang, Chanjuan, Yuan, Songli, Hao, Qinnan, Zhang, Xiaojuan, Qiu, Dezhen, Chen, Haifeng, Zhou, Xinan
Format:	Artikel
Sprache:	eng
Schlagworte:	Abiotic stress Agriculture Algorithms Biology Biology and Life Sciences Cultivars Developmental stages Ecology and Environmental Sciences Enzymes Gene expression Gene Expression Profiling Genes Genes, Plant Genetic aspects Genomics Glucosephosphate dehydrogenase Glycine max Glycine max - genetics Glycine max - growth & development Health aspects Infections Laboratories Metabolism Methods Nitrogen Physical Sciences Physiological aspects Plant virus diseases Polymerase chain reaction Proteins Real-Time Polymerase Chain Reaction - methods Research and Analysis Methods Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription Soybeans Stability analysis Stress Stresses Studies Transcription (Genetics) Viruses
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