Lysophosphatidic acid receptor mRNA levels in heart and white adipose tissue are associated with obesity in mice and humans
Lysophosphatidic acid (LPA) receptor signaling has been implicated in cardiovascular and obesity-related metabolic disease. However, the distribution and regulation of LPA receptors in the myocardium and adipose tissue remain unclear. This study aimed to characterize the mRNA expression of LPA recep...
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| Veröffentlicht in: | PloS one 2017-12, Vol.12 (12), p.e0189402-e0189402 |
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| creator | Brown, Amy Hossain, Intekhab Perez, Lester J Nzirorera, Carine Tozer, Kathleen D'Souza, Kenneth Trivedi, Purvi C Aguiar, Christie Yip, Alexandra M Shea, Jennifer Brunt, Keith R Legare, Jean-Francois Hassan, Ansar Pulinilkunnil, Thomas Kienesberger, Petra C |
| description | Lysophosphatidic acid (LPA) receptor signaling has been implicated in cardiovascular and obesity-related metabolic disease. However, the distribution and regulation of LPA receptors in the myocardium and adipose tissue remain unclear.
This study aimed to characterize the mRNA expression of LPA receptors (LPA1-6) in the murine and human myocardium and adipose tissue, and its regulation in response to obesity.
LPA receptor mRNA levels were determined by qPCR in i) heart ventricles, isolated cardiomyocytes, and perigonadal adipose tissue from chow or high fat-high sucrose (HFHS)-fed male C57BL/6 mice, ii) 3T3-L1 adipocytes and HL-1 cardiomyocytes under conditions mimicking gluco/lipotoxicity, and iii) human atrial and subcutaneous adipose tissue from non-obese, pre-obese, and obese cardiac surgery patients.
LPA1-6 were expressed in myocardium and white adipose tissue from mice and humans, except for LPA3, which was undetectable in murine adipocytes and human adipose tissue. Obesity was associated with increased LPA4, LPA5 and/or LPA6 levels in mice ventricles and cardiomyocytes, HL-1 cells exposed to high palmitate, and human atrial tissue. LPA4 and LPA5 mRNA levels in human atrial tissue correlated with measures of obesity. LPA5 mRNA levels were increased in HFHS-fed mice and insulin resistant adipocytes, yet were reduced in adipose tissue from obese patients. LPA4, LPA5, and LPA6 mRNA levels in human adipose tissue were negatively associated with measures of obesity and cardiac surgery outcomes. This study suggests that obesity leads to marked changes in LPA receptor expression in the murine and human heart and white adipose tissue that may alter LPA receptor signaling during obesity. |
| doi_str_mv | 10.1371/journal.pone.0189402 |
| format | Article |
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This study aimed to characterize the mRNA expression of LPA receptors (LPA1-6) in the murine and human myocardium and adipose tissue, and its regulation in response to obesity.
LPA receptor mRNA levels were determined by qPCR in i) heart ventricles, isolated cardiomyocytes, and perigonadal adipose tissue from chow or high fat-high sucrose (HFHS)-fed male C57BL/6 mice, ii) 3T3-L1 adipocytes and HL-1 cardiomyocytes under conditions mimicking gluco/lipotoxicity, and iii) human atrial and subcutaneous adipose tissue from non-obese, pre-obese, and obese cardiac surgery patients.
LPA1-6 were expressed in myocardium and white adipose tissue from mice and humans, except for LPA3, which was undetectable in murine adipocytes and human adipose tissue. Obesity was associated with increased LPA4, LPA5 and/or LPA6 levels in mice ventricles and cardiomyocytes, HL-1 cells exposed to high palmitate, and human atrial tissue. LPA4 and LPA5 mRNA levels in human atrial tissue correlated with measures of obesity. LPA5 mRNA levels were increased in HFHS-fed mice and insulin resistant adipocytes, yet were reduced in adipose tissue from obese patients. LPA4, LPA5, and LPA6 mRNA levels in human adipose tissue were negatively associated with measures of obesity and cardiac surgery outcomes. This study suggests that obesity leads to marked changes in LPA receptor expression in the murine and human heart and white adipose tissue that may alter LPA receptor signaling during obesity.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0189402</identifier><identifier>PMID: 29236751</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Adipocytes ; Adipose tissue ; Animal tissues ; Biochemistry ; Biology and Life Sciences ; Body fat ; Cardiomyocytes ; Correlation analysis ; Diagnosis ; Disease ; Gene expression ; Genetic aspects ; Glucose ; Heart ; Heart attacks ; Heart diseases ; Heart failure ; Heart surgery ; Homeostasis ; Hospitals ; Inflammation ; Insulin ; Insulin resistance ; Lipids ; Liver cancer ; Lysophosphatidic acid ; Measurement ; Medicine ; Medicine and Health Sciences ; Messenger RNA ; Metabolic disorders ; Mice ; Mimicry ; Molecular biology ; Myocardium ; Obesity ; Palmitic acid ; Patients ; Physiological aspects ; Receptors ; Risk factors ; Rodents ; Signaling ; Studies ; Sucrose ; Sugar ; Surgery</subject><ispartof>PloS one, 2017-12, Vol.12 (12), p.e0189402-e0189402</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Brown et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Brown et al 2017 Brown et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-576ab3da588057a3b389edc02b1cbea13bac6870c94b7004efb3676250406e823</citedby><cites>FETCH-LOGICAL-c692t-576ab3da588057a3b389edc02b1cbea13bac6870c94b7004efb3676250406e823</cites><orcidid>0000-0001-9643-8338</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728537/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728537/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29236751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Souza-Mello, Vanessa</contributor><creatorcontrib>Brown, Amy</creatorcontrib><creatorcontrib>Hossain, Intekhab</creatorcontrib><creatorcontrib>Perez, Lester J</creatorcontrib><creatorcontrib>Nzirorera, Carine</creatorcontrib><creatorcontrib>Tozer, Kathleen</creatorcontrib><creatorcontrib>D'Souza, Kenneth</creatorcontrib><creatorcontrib>Trivedi, Purvi C</creatorcontrib><creatorcontrib>Aguiar, Christie</creatorcontrib><creatorcontrib>Yip, Alexandra M</creatorcontrib><creatorcontrib>Shea, Jennifer</creatorcontrib><creatorcontrib>Brunt, Keith R</creatorcontrib><creatorcontrib>Legare, Jean-Francois</creatorcontrib><creatorcontrib>Hassan, Ansar</creatorcontrib><creatorcontrib>Pulinilkunnil, Thomas</creatorcontrib><creatorcontrib>Kienesberger, Petra C</creatorcontrib><title>Lysophosphatidic acid receptor mRNA levels in heart and white adipose tissue are associated with obesity in mice and humans</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Lysophosphatidic acid (LPA) receptor signaling has been implicated in cardiovascular and obesity-related metabolic disease. However, the distribution and regulation of LPA receptors in the myocardium and adipose tissue remain unclear.
This study aimed to characterize the mRNA expression of LPA receptors (LPA1-6) in the murine and human myocardium and adipose tissue, and its regulation in response to obesity.
LPA receptor mRNA levels were determined by qPCR in i) heart ventricles, isolated cardiomyocytes, and perigonadal adipose tissue from chow or high fat-high sucrose (HFHS)-fed male C57BL/6 mice, ii) 3T3-L1 adipocytes and HL-1 cardiomyocytes under conditions mimicking gluco/lipotoxicity, and iii) human atrial and subcutaneous adipose tissue from non-obese, pre-obese, and obese cardiac surgery patients.
LPA1-6 were expressed in myocardium and white adipose tissue from mice and humans, except for LPA3, which was undetectable in murine adipocytes and human adipose tissue. Obesity was associated with increased LPA4, LPA5 and/or LPA6 levels in mice ventricles and cardiomyocytes, HL-1 cells exposed to high palmitate, and human atrial tissue. LPA4 and LPA5 mRNA levels in human atrial tissue correlated with measures of obesity. LPA5 mRNA levels were increased in HFHS-fed mice and insulin resistant adipocytes, yet were reduced in adipose tissue from obese patients. LPA4, LPA5, and LPA6 mRNA levels in human adipose tissue were negatively associated with measures of obesity and cardiac surgery outcomes. This study suggests that obesity leads to marked changes in LPA receptor expression in the murine and human heart and white adipose tissue that may alter LPA receptor signaling during obesity.</description><subject>Acids</subject><subject>Adipocytes</subject><subject>Adipose tissue</subject><subject>Animal tissues</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Body fat</subject><subject>Cardiomyocytes</subject><subject>Correlation analysis</subject><subject>Diagnosis</subject><subject>Disease</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Glucose</subject><subject>Heart</subject><subject>Heart attacks</subject><subject>Heart diseases</subject><subject>Heart failure</subject><subject>Heart surgery</subject><subject>Homeostasis</subject><subject>Hospitals</subject><subject>Inflammation</subject><subject>Insulin</subject><subject>Insulin resistance</subject><subject>Lipids</subject><subject>Liver cancer</subject><subject>Lysophosphatidic acid</subject><subject>Measurement</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Messenger RNA</subject><subject>Metabolic disorders</subject><subject>Mice</subject><subject>Mimicry</subject><subject>Molecular biology</subject><subject>Myocardium</subject><subject>Obesity</subject><subject>Palmitic acid</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Receptors</subject><subject>Risk factors</subject><subject>Rodents</subject><subject>Signaling</subject><subject>Studies</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Surgery</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlokvZGGBY_BgYX1o_bkKZnphnapibp6uCfNzPTXaayFxJK2vR53-ScnJMkzzGaYyrwu60dXKeaeW87mCOcFxkiD5JzXFAy4wTRhyfvZ8kT77cIMZpz_jg5IwWhXDB8nvxZ7bzta-v7WgVTGZ0qbarUgYY-WJe2118WaQM30PjUdGkNyoVUdVX6qzYBUlWZ3npIg_F-iJ8uPt5bbVSAyJhQp7YEb8Jur26NhoO4HlrV-afJo7VqPDwb54vk-8cP3y4_z1ZXn5aXi9VM84KEGRNclbRSLM8RE4qWNC-g0oiUWJegMC2V5rlAushKgVAG6zIGxwlDGeKQE3qRvDz69o31csybl7gQPMOoEHkklkeismore2da5XbSKiMPC9ZtZIzb6AZkxgABZohzUWYYcF5qQaNRhhTLAOHo9X7cbSjbeE7oglPNxHT6pzO13NgbyQTJGRXR4M1o4OzPAXyQrfEamkZ1YIfDuQUhmFIU0Vf_oPdHN1IbFQMw3drGffXeVC4YznnBBKWRmt9DxVFBvLdYZGsT1yeCtxNBZAL8Dhs1eC-XX6__n736MWVfn7Cx4ppQe9sMwdjOT8HsCGpnvXewvksyRnLfI7fZkPsekWOPRNmL0wu6E902Bf0LM7UMFA</recordid><startdate>20171213</startdate><enddate>20171213</enddate><creator>Brown, 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acid receptor mRNA levels in heart and white adipose tissue are associated with obesity in mice and humans</title><author>Brown, Amy ; Hossain, Intekhab ; Perez, Lester J ; Nzirorera, Carine ; Tozer, Kathleen ; D'Souza, Kenneth ; Trivedi, Purvi C ; Aguiar, Christie ; Yip, Alexandra M ; Shea, Jennifer ; Brunt, Keith R ; Legare, Jean-Francois ; Hassan, Ansar ; Pulinilkunnil, Thomas ; Kienesberger, Petra C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-576ab3da588057a3b389edc02b1cbea13bac6870c94b7004efb3676250406e823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acids</topic><topic>Adipocytes</topic><topic>Adipose tissue</topic><topic>Animal tissues</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Body fat</topic><topic>Cardiomyocytes</topic><topic>Correlation analysis</topic><topic>Diagnosis</topic><topic>Disease</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Glucose</topic><topic>Heart</topic><topic>Heart attacks</topic><topic>Heart diseases</topic><topic>Heart failure</topic><topic>Heart surgery</topic><topic>Homeostasis</topic><topic>Hospitals</topic><topic>Inflammation</topic><topic>Insulin</topic><topic>Insulin resistance</topic><topic>Lipids</topic><topic>Liver cancer</topic><topic>Lysophosphatidic acid</topic><topic>Measurement</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Messenger RNA</topic><topic>Metabolic disorders</topic><topic>Mice</topic><topic>Mimicry</topic><topic>Molecular biology</topic><topic>Myocardium</topic><topic>Obesity</topic><topic>Palmitic acid</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Receptors</topic><topic>Risk 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Vanessa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lysophosphatidic acid receptor mRNA levels in heart and white adipose tissue are associated with obesity in mice and humans</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-12-13</date><risdate>2017</risdate><volume>12</volume><issue>12</issue><spage>e0189402</spage><epage>e0189402</epage><pages>e0189402-e0189402</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lysophosphatidic acid (LPA) receptor signaling has been implicated in cardiovascular and obesity-related metabolic disease. However, the distribution and regulation of LPA receptors in the myocardium and adipose tissue remain unclear.
This study aimed to characterize the mRNA expression of LPA receptors (LPA1-6) in the murine and human myocardium and adipose tissue, and its regulation in response to obesity.
LPA receptor mRNA levels were determined by qPCR in i) heart ventricles, isolated cardiomyocytes, and perigonadal adipose tissue from chow or high fat-high sucrose (HFHS)-fed male C57BL/6 mice, ii) 3T3-L1 adipocytes and HL-1 cardiomyocytes under conditions mimicking gluco/lipotoxicity, and iii) human atrial and subcutaneous adipose tissue from non-obese, pre-obese, and obese cardiac surgery patients.
LPA1-6 were expressed in myocardium and white adipose tissue from mice and humans, except for LPA3, which was undetectable in murine adipocytes and human adipose tissue. Obesity was associated with increased LPA4, LPA5 and/or LPA6 levels in mice ventricles and cardiomyocytes, HL-1 cells exposed to high palmitate, and human atrial tissue. LPA4 and LPA5 mRNA levels in human atrial tissue correlated with measures of obesity. LPA5 mRNA levels were increased in HFHS-fed mice and insulin resistant adipocytes, yet were reduced in adipose tissue from obese patients. LPA4, LPA5, and LPA6 mRNA levels in human adipose tissue were negatively associated with measures of obesity and cardiac surgery outcomes. This study suggests that obesity leads to marked changes in LPA receptor expression in the murine and human heart and white adipose tissue that may alter LPA receptor signaling during obesity.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29236751</pmid><doi>10.1371/journal.pone.0189402</doi><tpages>e0189402</tpages><orcidid>https://orcid.org/0000-0001-9643-8338</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-12, Vol.12 (12), p.e0189402-e0189402 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1976410978 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Adipocytes Adipose tissue Animal tissues Biochemistry Biology and Life Sciences Body fat Cardiomyocytes Correlation analysis Diagnosis Disease Gene expression Genetic aspects Glucose Heart Heart attacks Heart diseases Heart failure Heart surgery Homeostasis Hospitals Inflammation Insulin Insulin resistance Lipids Liver cancer Lysophosphatidic acid Measurement Medicine Medicine and Health Sciences Messenger RNA Metabolic disorders Mice Mimicry Molecular biology Myocardium Obesity Palmitic acid Patients Physiological aspects Receptors Risk factors Rodents Signaling Studies Sucrose Sugar Surgery |
| title | Lysophosphatidic acid receptor mRNA levels in heart and white adipose tissue are associated with obesity in mice and humans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T21%3A51%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lysophosphatidic%20acid%20receptor%20mRNA%20levels%20in%20heart%20and%20white%20adipose%20tissue%20are%20associated%20with%20obesity%20in%20mice%20and%20humans&rft.jtitle=PloS%20one&rft.au=Brown,%20Amy&rft.date=2017-12-13&rft.volume=12&rft.issue=12&rft.spage=e0189402&rft.epage=e0189402&rft.pages=e0189402-e0189402&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0189402&rft_dat=%3Cgale_plos_%3EA518695733%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1976410978&rft_id=info:pmid/29236751&rft_galeid=A518695733&rft_doaj_id=oai_doaj_org_article_45e0e150667b41e18bc7376440a54e01&rfr_iscdi=true |