454 pyrosequencing to describe microbial eukaryotic community composition, diversity and relative abundance: a test for marine haptophytes

Next generation sequencing of ribosomal DNA is increasingly used to assess the diversity and structure of microbial communities. Here we test the ability of 454 pyrosequencing to detect the number of species present, and assess the relative abundance in terms of cell numbers and biomass of protists...

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Veröffentlicht in:	PloS one 2013-09, Vol.8 (9), p.e74371-e74371
Hauptverfasser:	Egge, Elianne, Bittner, Lucie, Andersen, Tom, Audic, Stéphane, de Vargas, Colomban, Edvardsen, Bente
Format:	Artikel
Sprache:	eng
Schlagworte:	Abundance Algorithms Analysis Biodiversity Biomass Chrysochromulina Cloning Communities Community composition Deoxyribonucleic acid DNA DNA sequencing DNA, Complementary DNA, Ribosomal Ecology Environmental Sciences Filtration Gene sequencing Genomes Growth rate Haptophyta Haptophyta - classification Haptophyta - genetics High-Throughput Nucleotide Sequencing - methods High-Throughput Nucleotide Sequencing - standards Life Sciences Marine biology Metagenome Methods Microbial activity Microorganisms Microscopy Morphology Multivariate analysis Noise reduction Nucleotide sequencing Organisms Phylogenetics Phylogeny Primers Rarefaction Relative abundance Ribonucleic acid RNA Species Taxonomy
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