Intra-peritoneal hyperthermia combining α-galactosylceramide in the treatment of ovarian cancer
The purpose of this study was to investigate the anti-tumor effect and potential mechanisms of i.p. hyperthermia in combination with α-galactosylceramide (α-GalCer) for the treatment of ovarian cancer. In this study, immuno-competent tumor models were established using murine ovarian cancer cell lin...
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| Veröffentlicht in: | PloS one 2013-07, Vol.8 (7), p.e69336-e69336 |
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| creator | Wu, Chao-Chih Chuang, Yin-Ting Hsu, Yun-Ting Huang, Jung-Tang Wu, T-C Hung, Chien-Fu Yang, Yuh-Cheng Chang, Chih-Long |
| description | The purpose of this study was to investigate the anti-tumor effect and potential mechanisms of i.p. hyperthermia in combination with α-galactosylceramide (α-GalCer) for the treatment of ovarian cancer. In this study, immuno-competent tumor models were established using murine ovarian cancer cell lines and treated with i.p. hyperthermia combining α-GalCer. Th1/Th2 cytokine expression profiles in the serum, NK cell cytotoxicity and phagocytic activities of dendritic cells (DCs) were assayed. We also analyzed the number of CD8(+)/IFN-γ(+) tumor specific cytotoxic T cells, as well as the tumor growth based on depletion of lymphocyte sub-population. Therapeutic effect on those ovarian tumors was monitored by a non-invasive luminescent imaging system. Intra-peritoneal hyperthermia induced significant pro-inflammatory cytokines expression, and sustained the response of NK and DCs induced by α-GalCer treatment. The combination treatment enhanced the cytotoxic T lymphocyte (CTL) immune response in two mouse ovarian cancer models. This novel treatment modality by combination of hyperthermia and glycolipid provides a pronounced anti-tumor immune response and better survival. In conclusion, intra-peritoneal hyperthermia enhanced the pro-inflammatory cytokine secretion and phagocytic activity of DCs stimulated by α-GalCer. The subsequent CTL immune response induced by α-GalCer was further strengthened by combining with i.p. hyperthermia. Both innate and adaptive immunities were involved and resulted in a superior therapeutic effect in treating the ovarian cancer. |
| doi_str_mv | 10.1371/journal.pone.0069336 |
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In this study, immuno-competent tumor models were established using murine ovarian cancer cell lines and treated with i.p. hyperthermia combining α-GalCer. Th1/Th2 cytokine expression profiles in the serum, NK cell cytotoxicity and phagocytic activities of dendritic cells (DCs) were assayed. We also analyzed the number of CD8(+)/IFN-γ(+) tumor specific cytotoxic T cells, as well as the tumor growth based on depletion of lymphocyte sub-population. Therapeutic effect on those ovarian tumors was monitored by a non-invasive luminescent imaging system. Intra-peritoneal hyperthermia induced significant pro-inflammatory cytokines expression, and sustained the response of NK and DCs induced by α-GalCer treatment. The combination treatment enhanced the cytotoxic T lymphocyte (CTL) immune response in two mouse ovarian cancer models. This novel treatment modality by combination of hyperthermia and glycolipid provides a pronounced anti-tumor immune response and better survival. In conclusion, intra-peritoneal hyperthermia enhanced the pro-inflammatory cytokine secretion and phagocytic activity of DCs stimulated by α-GalCer. The subsequent CTL immune response induced by α-GalCer was further strengthened by combining with i.p. hyperthermia. Both innate and adaptive immunities were involved and resulted in a superior therapeutic effect in treating the ovarian cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0069336</identifier><identifier>PMID: 23935988</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal models ; Animals ; Anticancer properties ; Antigens ; Biology ; Cancer ; CD8 antigen ; CD8-Positive T-Lymphocytes - drug effects ; CD8-Positive T-Lymphocytes - metabolism ; Cell Death - drug effects ; Cell Line, Tumor ; Chemotherapy ; Cytokines ; Cytokines - blood ; Cytokines - secretion ; Cytotoxicity ; Dendritic cells ; Dendritic Cells - drug effects ; Dendritic Cells - metabolism ; Diabetes ; Disease Models, Animal ; Electrical engineering ; Female ; Fever ; Galactosylceramide ; Galactosylceramides - pharmacology ; Galactosylceramides - therapeutic use ; Gynecology ; Heat shock proteins ; Hospitals ; Hyperthermia ; Hyperthermia, Induced ; Immune response ; Immune system ; Immunology ; Inflammation ; Inflammation Mediators - metabolism ; Interferon ; Ligands ; Lymphocytes ; Lymphocytes T ; Medical research ; Medicine ; Metastasis ; Mice ; Natural killer cells ; Obstetrics ; Ovarian cancer ; Ovarian carcinoma ; Ovarian Neoplasms - drug therapy ; Ovarian Neoplasms - pathology ; Peritoneum ; Peritoneum - drug effects ; Peritoneum - pathology ; Phagocytes ; Phagocytosis - drug effects ; T cell receptors ; Th1 Cells - drug effects ; Th1 Cells - metabolism ; Th2 Cells - drug effects ; Th2 Cells - metabolism ; Toxicity ; Tumor cell lines ; Tumors ; γ-Interferon</subject><ispartof>PloS one, 2013-07, Vol.8 (7), p.e69336-e69336</ispartof><rights>2013 Wu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Wu et al 2013 Wu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-ecafe0160ff98355eea6a273a8a9ed48ab508650213e54cbc33aa85aceab71853</citedby><cites>FETCH-LOGICAL-c526t-ecafe0160ff98355eea6a273a8a9ed48ab508650213e54cbc33aa85aceab71853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720534/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720534/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23935988$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tsuji, Moriya</contributor><creatorcontrib>Wu, Chao-Chih</creatorcontrib><creatorcontrib>Chuang, Yin-Ting</creatorcontrib><creatorcontrib>Hsu, Yun-Ting</creatorcontrib><creatorcontrib>Huang, Jung-Tang</creatorcontrib><creatorcontrib>Wu, T-C</creatorcontrib><creatorcontrib>Hung, Chien-Fu</creatorcontrib><creatorcontrib>Yang, Yuh-Cheng</creatorcontrib><creatorcontrib>Chang, Chih-Long</creatorcontrib><title>Intra-peritoneal hyperthermia combining α-galactosylceramide in the treatment of ovarian cancer</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The purpose of this study was to investigate the anti-tumor effect and potential mechanisms of i.p. hyperthermia in combination with α-galactosylceramide (α-GalCer) for the treatment of ovarian cancer. In this study, immuno-competent tumor models were established using murine ovarian cancer cell lines and treated with i.p. hyperthermia combining α-GalCer. Th1/Th2 cytokine expression profiles in the serum, NK cell cytotoxicity and phagocytic activities of dendritic cells (DCs) were assayed. We also analyzed the number of CD8(+)/IFN-γ(+) tumor specific cytotoxic T cells, as well as the tumor growth based on depletion of lymphocyte sub-population. Therapeutic effect on those ovarian tumors was monitored by a non-invasive luminescent imaging system. Intra-peritoneal hyperthermia induced significant pro-inflammatory cytokines expression, and sustained the response of NK and DCs induced by α-GalCer treatment. The combination treatment enhanced the cytotoxic T lymphocyte (CTL) immune response in two mouse ovarian cancer models. This novel treatment modality by combination of hyperthermia and glycolipid provides a pronounced anti-tumor immune response and better survival. In conclusion, intra-peritoneal hyperthermia enhanced the pro-inflammatory cytokine secretion and phagocytic activity of DCs stimulated by α-GalCer. The subsequent CTL immune response induced by α-GalCer was further strengthened by combining with i.p. hyperthermia. Both innate and adaptive immunities were involved and resulted in a superior therapeutic effect in treating the ovarian cancer.</description><subject>Animal models</subject><subject>Animals</subject><subject>Anticancer properties</subject><subject>Antigens</subject><subject>Biology</subject><subject>Cancer</subject><subject>CD8 antigen</subject><subject>CD8-Positive T-Lymphocytes - drug effects</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cell Death - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Chemotherapy</subject><subject>Cytokines</subject><subject>Cytokines - blood</subject><subject>Cytokines - secretion</subject><subject>Cytotoxicity</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - metabolism</subject><subject>Diabetes</subject><subject>Disease Models, Animal</subject><subject>Electrical engineering</subject><subject>Female</subject><subject>Fever</subject><subject>Galactosylceramide</subject><subject>Galactosylceramides - pharmacology</subject><subject>Galactosylceramides - therapeutic use</subject><subject>Gynecology</subject><subject>Heat shock proteins</subject><subject>Hospitals</subject><subject>Hyperthermia</subject><subject>Hyperthermia, Induced</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Interferon</subject><subject>Ligands</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Natural killer cells</subject><subject>Obstetrics</subject><subject>Ovarian cancer</subject><subject>Ovarian carcinoma</subject><subject>Ovarian Neoplasms - drug therapy</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Peritoneum</subject><subject>Peritoneum - drug effects</subject><subject>Peritoneum - pathology</subject><subject>Phagocytes</subject><subject>Phagocytosis - drug effects</subject><subject>T cell receptors</subject><subject>Th1 Cells - drug effects</subject><subject>Th1 Cells - metabolism</subject><subject>Th2 Cells - drug effects</subject><subject>Th2 Cells - metabolism</subject><subject>Toxicity</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><subject>γ-Interferon</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUsuOEzEQHCEQuwT-AMFIXLhM8GPs8VyQ0IpHpJW4wNn0OD2JI48dbGelfBY_wjfhkNnVLuLkdru6urtcVfWSkiXlHX23C4fowS33weOSENlzLh9Vl7TnrJGM8Mf34ovqWUo7QgRXUj6tLhjvueiVuqx-rHyO0Owx2lyIwNXbY7nkLcbJQm3CNFhv_ab-_avZgAOTQzo6gxEmu8ba-rpA6xwR8oQ-12Gsww1EC7424AvuefVkBJfwxXwuqu-fPn67-tJcf_28uvpw3RjBZG7QwIiESjKOveJCIIIE1nFQ0OO6VTAIoqQgjHIUrRkM5wBKgEEYOqoEX1Svz7x7F5KexUma9l0rmVJFnkW1OiPWAXZ6H-0E8agDWP03EeJGQ8zWONQ9UMLEwJgipXdLeoZ8TZAMJVKE0ML1fu52GCZcGzyp6B6QPnzxdqs34UbzjpVfaAvB25kghp8HTFlPNhl0DjyGQ5m7ZUUN2tGuQN_8A_3_du0ZZWJIKeJ4Nwwl-mSY2yp9MoyeDVPKXt1f5K7o1iH8D3vLwNM</recordid><startdate>20130723</startdate><enddate>20130723</enddate><creator>Wu, Chao-Chih</creator><creator>Chuang, Yin-Ting</creator><creator>Hsu, Yun-Ting</creator><creator>Huang, Jung-Tang</creator><creator>Wu, T-C</creator><creator>Hung, Chien-Fu</creator><creator>Yang, Yuh-Cheng</creator><creator>Chang, Chih-Long</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130723</creationdate><title>Intra-peritoneal hyperthermia combining α-galactosylceramide in the treatment of ovarian cancer</title><author>Wu, Chao-Chih ; Chuang, Yin-Ting ; Hsu, Yun-Ting ; Huang, Jung-Tang ; Wu, T-C ; Hung, Chien-Fu ; Yang, Yuh-Cheng ; Chang, Chih-Long</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-ecafe0160ff98355eea6a273a8a9ed48ab508650213e54cbc33aa85aceab71853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Anticancer properties</topic><topic>Antigens</topic><topic>Biology</topic><topic>Cancer</topic><topic>CD8 antigen</topic><topic>CD8-Positive T-Lymphocytes - drug effects</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cell Death - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Chemotherapy</topic><topic>Cytokines</topic><topic>Cytokines - blood</topic><topic>Cytokines - secretion</topic><topic>Cytotoxicity</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - metabolism</topic><topic>Diabetes</topic><topic>Disease Models, Animal</topic><topic>Electrical engineering</topic><topic>Female</topic><topic>Fever</topic><topic>Galactosylceramide</topic><topic>Galactosylceramides - pharmacology</topic><topic>Galactosylceramides - therapeutic use</topic><topic>Gynecology</topic><topic>Heat shock proteins</topic><topic>Hospitals</topic><topic>Hyperthermia</topic><topic>Hyperthermia, Induced</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Interferon</topic><topic>Ligands</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Natural killer cells</topic><topic>Obstetrics</topic><topic>Ovarian cancer</topic><topic>Ovarian carcinoma</topic><topic>Ovarian Neoplasms - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Chao-Chih</au><au>Chuang, Yin-Ting</au><au>Hsu, Yun-Ting</au><au>Huang, Jung-Tang</au><au>Wu, T-C</au><au>Hung, Chien-Fu</au><au>Yang, Yuh-Cheng</au><au>Chang, Chih-Long</au><au>Tsuji, Moriya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intra-peritoneal hyperthermia combining α-galactosylceramide in the treatment of ovarian cancer</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-07-23</date><risdate>2013</risdate><volume>8</volume><issue>7</issue><spage>e69336</spage><epage>e69336</epage><pages>e69336-e69336</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The purpose of this study was to investigate the anti-tumor effect and potential mechanisms of i.p. hyperthermia in combination with α-galactosylceramide (α-GalCer) for the treatment of ovarian cancer. In this study, immuno-competent tumor models were established using murine ovarian cancer cell lines and treated with i.p. hyperthermia combining α-GalCer. Th1/Th2 cytokine expression profiles in the serum, NK cell cytotoxicity and phagocytic activities of dendritic cells (DCs) were assayed. We also analyzed the number of CD8(+)/IFN-γ(+) tumor specific cytotoxic T cells, as well as the tumor growth based on depletion of lymphocyte sub-population. Therapeutic effect on those ovarian tumors was monitored by a non-invasive luminescent imaging system. Intra-peritoneal hyperthermia induced significant pro-inflammatory cytokines expression, and sustained the response of NK and DCs induced by α-GalCer treatment. The combination treatment enhanced the cytotoxic T lymphocyte (CTL) immune response in two mouse ovarian cancer models. This novel treatment modality by combination of hyperthermia and glycolipid provides a pronounced anti-tumor immune response and better survival. In conclusion, intra-peritoneal hyperthermia enhanced the pro-inflammatory cytokine secretion and phagocytic activity of DCs stimulated by α-GalCer. The subsequent CTL immune response induced by α-GalCer was further strengthened by combining with i.p. hyperthermia. Both innate and adaptive immunities were involved and resulted in a superior therapeutic effect in treating the ovarian cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23935988</pmid><doi>10.1371/journal.pone.0069336</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1974628869 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animal models Animals Anticancer properties Antigens Biology Cancer CD8 antigen CD8-Positive T-Lymphocytes - drug effects CD8-Positive T-Lymphocytes - metabolism Cell Death - drug effects Cell Line, Tumor Chemotherapy Cytokines Cytokines - blood Cytokines - secretion Cytotoxicity Dendritic cells Dendritic Cells - drug effects Dendritic Cells - metabolism Diabetes Disease Models, Animal Electrical engineering Female Fever Galactosylceramide Galactosylceramides - pharmacology Galactosylceramides - therapeutic use Gynecology Heat shock proteins Hospitals Hyperthermia Hyperthermia, Induced Immune response Immune system Immunology Inflammation Inflammation Mediators - metabolism Interferon Ligands Lymphocytes Lymphocytes T Medical research Medicine Metastasis Mice Natural killer cells Obstetrics Ovarian cancer Ovarian carcinoma Ovarian Neoplasms - drug therapy Ovarian Neoplasms - pathology Peritoneum Peritoneum - drug effects Peritoneum - pathology Phagocytes Phagocytosis - drug effects T cell receptors Th1 Cells - drug effects Th1 Cells - metabolism Th2 Cells - drug effects Th2 Cells - metabolism Toxicity Tumor cell lines Tumors γ-Interferon |
| title | Intra-peritoneal hyperthermia combining α-galactosylceramide in the treatment of ovarian cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T20%3A13%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intra-peritoneal%20hyperthermia%20combining%20%CE%B1-galactosylceramide%20in%20the%20treatment%20of%20ovarian%20cancer&rft.jtitle=PloS%20one&rft.au=Wu,%20Chao-Chih&rft.date=2013-07-23&rft.volume=8&rft.issue=7&rft.spage=e69336&rft.epage=e69336&rft.pages=e69336-e69336&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0069336&rft_dat=%3Cproquest_plos_%3E1420161717%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1974628869&rft_id=info:pmid/23935988&rft_doaj_id=oai_doaj_org_article_9a1025b2280d484092e3d0e0b0928001&rfr_iscdi=true |