The perfect host: a mouse host embryo facilitating more efficient germ line transmission of genetically modified embryonic stem cells

There is a continual need to improve efficiency in creating precise genetic modifications in mice using embryonic stem cells (ESCs). We describe a novel approach resulting in 100% germline transmission from competent injected ESCs. We developed an F1 mouse host embryo (Perfect Host, PH) that selecti...

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Veröffentlicht in:	PloS one 2013-07, Vol.8 (7), p.e67826-e67826
Hauptverfasser:	Taft, Robert A, Low, Benjamin E, Byers, Shannon L, Murray, Stephen A, Kutny, Peter, Wiles, Michael V
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biology Blastocyst - cytology Blastocyst - metabolism Blastocysts Breeding Cells, Cultured Chimeras Competition Consortia Diphtheria Diphtheria toxin Embryo cells Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Embryos Female Genetic aspects Genetic Engineering - methods Genetic modification Genetically modified animals Genomes Genotype Genotyping Germ cells Germ Cells - cytology Germ Cells - metabolism Laboratories Male Males Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Knockout Mice, Transgenic Offspring Physiological aspects Rodents Stem cell transplantation Stem cells Toxins
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